Development of a total Lagrangian SPH code for the simulation of solids under dynamic loading

This thesis makes use of an alternative SPH formulation, the Total Lagrangian formulation, to characterise dynamic events in solids and to achieve the proposed objectives outlined in Chapter 1. The structure is as follows: Chapter 1, Introduction, describes the motivation for this research and outlines the objectives and the structure of this thesis. Chapter 2, SPH fundamentals, supplies the standard procedure to generate particle equations and provides a comprehensive summary of gradient approximation formulae in SPH. The discretised SPH form of the conservation laws is included here. Chapter 3, SPH drawbacks: describes the limitations of SPH such as particle deficiency, consistency, zero energy modes, treatment of boundaries and the tensile instability problem. A rigorous stability analysis of continua and SPH particle equations is also presented in this chapter. Chapter 4, Total Lagrangian SPH. Continuum Mechanics considerations are discussed here; detailed derivations of SPH equations in a total Lagrangian framework are given together with potential corrections to the total Lagrangian SPH equations. Chapter 5, Total Lagrangian SPH algorithms and their implementation using FORTRAN. This chapter gives a brief introduction to explicit codes. It also provides flow charts describing the Total Lagrangian algorithms and their integration into the MCM code. Chapter 6, Total Lagrangian SPH code validation. This chapter includes problems of varying degrees of complexity. Examples are provided to illustrate how the Total Lagrangian SPH code compares to a conventional collocational SPH code. Cases are supplied for which the analytical solution is known, and the results compared with the SPH approximations in order to show the accuracy of the approximation. Some examples are supplied which provide a direct comparison between SPH and non linear FE results and SPH and experimental results. Chapter 7, Alternative formulation of SPH equations and improvements to the standard MCM code: Various modifications to the standard SPH code are presented. These modifications include the implementation of subroutines that make use of an alternative approach to ensure the conservation of mass law is met locally at every particle. The introduction of XSPH to achieve further stabilisation of the code was also carried out and some examples are provided. The theory behind an alternative form of the conservation of mass equation as proposed by Belytschko [4] is explained and its implementation into the SPH code is assessed through examples. Also, an alternative formulation of SPH equations based on the general theory of mixed Lagrangian-Eulerian formulations [35] is presented: these equations could serve as the foundation for future research in this field. Chapter 8, Conclusions are presented in this chapter. A brief literature review is provided at the beginning of each chapter as a means of introduction to the topic and a concise summary outlines the main points discussed.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Phagocytosis-Dependent Ketogenesis in Retinal Pigment Epithelium

Daily, the retinal pigment epithelium (RPE) ingests a bolus of lipid and protein in the form of phagocytized photoreceptor outer segments (OS). The RPE, like the liver, expresses enzymes required for fatty acid oxidation and ketogenesis. This suggests that these pathways play a role in the disposal of lipids from ingested OS, as well as providing a mechanism for recycling metabolic intermediates back to the outer retina. In this study, we examined whether OS phagocytosis was linked to ketogenesis. We found increased levels of β-hydroxybutyrate (β-HB) in the apical medium following ingestion of OS by human fetal RPE and ARPE19 cells cultured on Transwell inserts. No increase in ketogenesis was observed following ingestion of oxidized OS or latex beads. Our studies further defined the connection between OS phagocytosis and ketogenesis in wild-type mice and mice with defects in phagosome maturation using a mouse RPE explant model. In explant studies, the levels of β-HB released were temporally correlated with OS phagocytic burst after light onset. In the Mreg-/- mouse where phagosome maturation is delayed, there was a temporal shift in the release of β-HB. An even more pronounced shift in maximal β-HB production was observed in the Abca4-/- RPE, in which loss of the ATP-binding cassette A4 transporter results in defective phagosome processing and accumulation of lipid debris. These studies suggest that FAO and ketogenesis are key to supporting the metabolism of the RPE and preventing the accumulation of lipids that lead to oxidative stress and mitochondrial dysfunction. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc

Phagocytosis-Dependent Ketogenesis in Retinal Pigment Epithelium

Daily, the retinal pigment epithelium (RPE) ingests a bolus of lipid and protein in the form of phagocytized photoreceptor outer segments (OS). The RPE, like the liver, expresses enzymes required for fatty acid oxidation and ketogenesis. This suggests that these pathways play a role in the disposal of lipids from ingested OS, as well as providing a mechanism for recycling metabolic intermediates back to the outer retina. In this study, we examined whether OS phagocytosis was linked to ketogenesis. We found increased levels of β-hydroxybutyrate (β-HB) in the apical medium following ingestion of OS by human fetal RPE and ARPE19 cells cultured on Transwell inserts. No increase in ketogenesis was observed following ingestion of oxidized OS or latex beads. Our studies further defined the connection between OS phagocytosis and ketogenesis in wild-type mice and mice with defects in phagosome maturation using a mouse RPE explant model. In explant studies, the levels of β-HB released were temporally correlated with OS phagocytic burst after light onset. In the Mreg−/− mouse where phagosome maturation is delayed, there was a temporal shift in the release of β-HB. An even more pronounced shift in maximal β-HB production was observed in the Abca4−/− RPE, in which loss of the ATP-binding cassette A4 transporter results in defective phagosome processing and accumulation of lipid debris. These studies suggest that FAO and ketogenesis are key to supporting the metabolism of the RPE and preventing the accumulation of lipids that lead to oxidative stress and mitochondrial dysfunction

The Contribution of Melanoregulin to Microtubule-Associated Protein 1 Light Chain 3 (LC3) Associated Phagocytosis in Retinal Pigment Epithelium

A main requisite in the phagocytosis of ingested material is a coordinated series of maturation steps which lead to the degradation of ingested cargo. Photoreceptor outer segment (POS) renewal involves phagocytosis of the distal disk membranes by the retinal pigment epithelium (RPE). Previously, we identified melanoregulin (MREG) as an intracellular cargo-sorting protein required for the degradation of POS disks. Here, we provide evidence that MREG-dependent processing links both autophagic and phagocytic processes in LC3-associated phagocytosis (LAP). Ingested POS phagosomes are associated with endogenous LC3 and MREG. The LC3 association with POSs exhibited properties of LAP; it was independent of rapamycin pretreatment, but dependent on Atg5. Loss of MREG resulted in a decrease in the extent of LC3-POS association. Studies using DQ™-BSA suggest that loss of MREG does not compromise the association and fusion of LC3-positive phagosomes with lysosomes. Furthermore, the mechanism of MREG action is likely through a protein complex that includes LC3, as determined by colocalization and immunoprecipitation in both RPE cells and macrophages. We posit that MREG participates in coordinating the association of phagosomes with LC3 for content degradation with the loss of MREG leading to phagosome accumulation. © 2014, Springer Science+Business Media New York

The Contribution of Melanoregulin to Microtubule-Associated Protein 1 Light Chain 3 (LC3) Associated Phagocytosis in Retinal Pigment Epithelium

A main requisite in the phagocytosis of ingestedmaterial is a coordinated series of maturation steps which leadto the degradation of ingested cargo. Photoreceptor outersegment (POS) renewal involves phagocytosis of the distaldisk membranes by the retinal pigment epithelium (RPE).Previously, we identified melanoregulin (MREG) as an intra-cellular cargo-sorting protein required for the degradation ofPOS disks. Here, we provide evidence that MREG-dependentprocessing links both autophagic and phagocytic processes inLC3-associated phagocytosis (LAP). Ingested POSphagosomes are associated with endogenous LC3 andMREG. The LC3 association with POSs exhibited propertiesof LAP; it was independent of rapamycin pretreatment, butdependent on Atg5. Loss of MREG resulted in a decrease inthe extent of LC3-POS association. Studies using DQ™-BSAsuggest that loss of MREG does not compromise the associ-ation and fusion of LC3-positive phagosomes with lysosomes.Furthermore, the mechanism of MREG action is likelythrough a protein complex that includes LC3, as determinedby colocalization and immunoprecipitation in both RPE cellsand macrophages. We posit that MREG participates incoordinating the association of phagosomes with LC3 forcontent degradation with the loss of MREG leading tophagosome accumulation

Microtubule-Associated Protein 1 Light Chain 3B, (LC3B) is Necessary to Maintain Lipid-Mediated Homeostasis in the Retinal Pigment Epithelium

Like other neurons, retinal cells utilize autophagic pathways to maintain cell homeostasis. The mammalian retina relies on heterophagy and selective autophagy to efficiently degrade and metabolize ingested lipids with disruption in autophagy associated degradation contributing to age related retinal disorders. The retinal pigment epithelium (RPE) supports photoreceptor cell renewal by daily phagocytosis of shed photoreceptor outer segments (OS). The daily ingestion of these lipid-rich OS imposes a constant degradative burden on these terminally differentiated cells. These cells rely on Microtubule-Associated Protein 1 Light Chain 3 (LC3) family of proteins for phagocytic clearance of the ingested OS. The LC3 family comprises of three highly homologous members, MAP1LC3A (LC3A), MAP1LC3B (LC3B), and MAP1LC3C (LC3C). The purpose of this study was to determine whether the LC3B isoform plays a specific role in maintaining RPE lipid homeostasis. We examined the RPE and retina of the LC3B-/- mouse as a function of age using in vivo ocular imaging and electroretinography coupled with ex vivo, lipidomic analyses of lipid mediators, assessment of bisretinoids as well as imaging of lipid aggregates. Deletion of LC3B resulted in defects within the RPE including increased phagosome accumulation, decreased fatty acid oxidation and a subsequent increase in RPE and sub-RPE lipid deposits. Age-dependent RPE changes included elevated levels of oxidized cholesterol, deposition of 4-HNE lipid peroxidation products, bisretinoid lipofuscin accumulation, and subretinal migration of microglia, collectively likely contributing to loss of retinal function. These observations are consistent with a critical role for LC3B-dependent processes in the maintenance of normal lipid homeostasis in the aging RPE, and suggest that LC3 isoform specific disruption in autophagic processes contribute to AMD-like pathogenesis. © 2018 Dhingra, Bell, Peachey, Daniele, Reyes-Reveles, Sharp, Jun, Bazan, Sparrow, Kim, Philp and Boesze-Battaglia

El autoensamblaje en la escala macroscópica

We present a review of the state of the art of self-assembly on a macroscopic scale, covering ten years, from 2012 to 2022. The study begins by mentioning its discovery, definition, fundamental characteristics, and the two main types of self-assembly. Next, we propose the opportunity to implement the autonomous assembly process as a construction alternative on the macroscopic scale, especially in engineering. In a complementary way, we expose the advantages of self-assembly on a macroscopic scale and the challenges regarding its physical and virtual implementation. Finally, we cite relevant research to finish mentioning the opportunities for future work and conclusions about self-assembly.Se presenta una revisión del estado del arte del autoensamblaje en escala macroscópica, abarcando diez años, de 2012 a 2022. El estudio inicia mencionando su descubrimiento, definición, características fundamentales, y los dos tipos principales de autoensamblaje. En seguida se plantea la oportunidad de implementar el proceso de ensamblaje autónomo como alternativa de construcción en la escala macroscópica, especialmente en la ingeniería. De modo complementario, se exponen las ventajas del autoensamblaje en escala macroscópica y los desafíos respecto a su implementación física y virtual. Finalmente, se citan investigaciones relevantes para terminar mencionando las oportunidades de trabajo futuro y conclusiones acerca del autoensamblaje

Mechanical Design of Self-Reconfiguring 4D-Printed OrigamiSats: A New Concept for Solar Sailing

In this article, a self-reconfiguring OrigamiSat concept is presented. The reconfiguration of the proposed OrigamiSat is triggered by combining the effect of 4D material (i.e. origami’s edges) and changes in the local surface optical properties (i.e., origami’s facets) to harness the solar radiation pressure acceleration. The proposed OrigamiSat uses the principle of solar sailing to enhance the effect of the Sun radiation to generate momentum on the Aluminised Kapton (Al-Kapton) origami surface by transitioning from mirror-like to diffusely reflecting optical properties of each individual facet. Numerical simulations have demonstrated that local changes in the optical properties can trigger reconfiguration. A minimum of 1-m edge size facet is required for a thick-origami to generate enough forces from the Sun radiation. The thick-origami pattern is 3D-printed directly on a thin Al-Kapton film (the solar sail substrate which is highly reflective). An elastic filament (thermoplastic polyurethane TPU) showed best performance when printing directly on the Al-Kapton and the Acrylonitrile Butadiene Styrene with carbon fiber reinforcement (ABS/cc) is added to augment the origami mechanical properties. The 4D material (shape memory polymer) is integrated only at specific edges to achieve self-deployment by applying heat. Two different folding mechanisms were studied: 1) the cartilage-like, where the hinge is made combining the TPU and the 4D material which make the mounts or valleys fully stretchable, and 2) the mechanical hinge, where simple hinges are made solely of ABS/cc. Numerical simulations have demonstrated that the cartilage-like hinge is the most suitable design for light-weight reconfigurable OrigamiSat when using the solar radiation pressure acceleration. We have used build-in electric board to heat up the 4D material and trigger the folding. We envisage embedding the heat wire within the 4D hinge in the future.</jats:p

Microtubule-Associated Protein 1 Light Chain 3B, (LC3B) Is Necessary to Maintain Lipid-Mediated Homeostasis in the Retinal Pigment Epithelium

Like other neurons, retinal cells utilize autophagic pathways to maintain cell homeostasis. The mammalian retina relies on heterophagy and selective autophagy to efficiently degrade and metabolize ingested lipids with disruption in autophagy associated degradation contributing to age related retinal disorders. The retinal pigment epithelium (RPE) supports photoreceptor cell renewal by daily phagocytosis of shed photoreceptor outer segments (OS). The daily ingestion of these lipid-rich OS imposes a constant degradative burden on these terminally differentiated cells. These cells rely on Microtubule-Associated Protein 1 Light Chain 3 (LC3) family of proteins for phagocytic clearance of the ingested OS. The LC3 family comprises of three highly homologous members, MAP1LC3A (LC3A), MAP1LC3B (LC3B), and MAP1LC3C (LC3C). The purpose of this study was to determine whether the LC3B isoform plays a specific role in maintaining RPE lipid homeostasis. We examined the RPE and retina of the LC3B-/- mouse as a function of age using in vivo ocular imaging and electroretinography coupled with ex vivo, lipidomic analyses of lipid mediators, assessment of bisretinoids as well as imaging of lipid aggregates. Deletion of LC3B resulted in defects within the RPE including increased phagosome accumulation, decreased fatty acid oxidation and a subsequent increase in RPE and sub-RPE lipid deposits. Age-dependent RPE changes included elevated levels of oxidized cholesterol, deposition of 4-HNE lipid peroxidation products, bisretinoid lipofuscin accumulation, and subretinal migration of microglia, collectively likely contributing to loss of retinal function. These observations are consistent with a critical role for LC3B-dependent processes in the maintenance of normal lipid homeostasis in the aging RPE, and suggest that LC3 isoform specific disruption in autophagic processes contribute to AMD-like pathogenesis

IV Foro Internacional de Ganadería Sustentable: conectividad ecosistémica y articulación territorial hacia la Agenda 2030

En este espacio plural de análisis y reflexiones, buscamos articular conceptos y posibilidades para los territorios de montaña del centro de México, dando importancia a medios de vida vinculados a la ganadería y su interacción ecosistémica, fundamentado en innovaciones, casos de éxito e iniciativas emblemáticas nacionales e internacionales. La socialización de experiencias es uno de los pilares para transitar hacia la sostenibilidad de los sistemas productivos ganaderos: compartir logros e iniciativas, crear sinergias e identificar vulnerabilidades desde distintos enfoques.GIZ, Agencia de Cooperación Aleman