A Simple, Quick, and Precise Procedure for the Determination of Water in Organic Solvents

A procedure for the UV/VIS-spectroscopic determination of water by the use of a solvatochromic pyridiniumphenolate betaine is given. The water content of organic solvents is calculated by a two parameter equation from λmax of the dye. A typical, detection limit is of the order of 1 mg in 1 ml solvent for routine spectrometers. The parameters for the determination of water are given for a number of commonly used solvents

An Experimental Investigation in a Turbulent Channel Flow With a Thick Viscous Sublayer (Hot-Film Measurements in Oil)

In a turbulent channel-flow hot-film measurements have been made. To achieve a sublayer thickness of approximately 1 cm at y+ = 10, oil was used. The Reynolds numbers used for the investigations were 5,600 and 8,200 based on the channel-width of 22 cm and the channel center-line velocity. In the vicinity of the wall, y+ \u3c 0.1, the u\u27-fluctuations were found to be proportional to the wall distance, y+. The u\u27-values obtained with a hot-film probe for y+ \u3e 0.7 were all greater than those obtained with a hot-film wall probe, but extrapolation of the data from the movable hot-film probe to the wall gave good agreement with the data from the flush-mounted wall-film probe. The instantaneous values of the u\u27-fluctuations in the region 0 \u3c y+ \u3c 5 are very similar to the instantaneous values of the wall-gradient, but there is a time shift which is proportional to the wall distance, y+. Disturbances in the flow in this region were observed to be convected with a constant velocity toward the wall. The mean value of the convection velocity was found to be approximately equal to the friction velocity, uT. The Reynolds stress was found to be intermittent in the vicinity of the wall with high peak to mean ratios. It was found that the probability density of the instantaneous streamwise velocity is skewed for all y+ values except y+ ≈ 13. For y+ \u3c 13 the most probable instantaneous velocity is less than the mean velocity; for y+ \u3e 13 the opposite was found

On the recording of turbulent longitudinal and transverse fluctuations

A thorough understanding of the turbulent flow movements cannot be arrived at from investigations of the temporal mean values of the flows alone. Study of the fluctuation phenomena themselves is indispensable. Thus turbulence research entered a new promising stage when investigators started performing fluctuation measurements and basing theories on those measurements. The investigations of fluctuations carried out so far refer almost exclusively to the so-called isotropic turbulence. It represents a damping phenomenon; it is the simplest type of turbulence where merely the longitudinal fluctuations need to be measured. However, it is precisely nonisotropic turbulence which involves the most essential problem of the turbulent exchange movement and the turbulent apparent friction. Investigation of nonisotropic turbulence requires measurement of longitudinal and transverse fluctuations

The alpha 1/beta 1 and alpha 6/beta 1 integrin heterodimers mediate cell attachment to distinct sites on laminin.

This study was undertaken to determine the roles of individual alpha/beta 1 integrin heterodimers in promoting cellular interactions with the different attachment-promoting domains of laminin (LN). To do this, antibodies to the integrin beta 1 subunit or to specific integrin alpha subunits were tested for effects on cell attachment to LN, to elastase fragments E1-4 and E1, derived from the short arms and core of LN's cruciform structure, and to fragment E8 derived from the long arm of this structure. The human JAR choriocarcinoma cells used in this study attached to LN and to fragments E1 and E8. Attachment to E1-4 required a much higher substrate coating concentration, suggesting that it is a poor substrate for JAR cell attachment. The ability of cells to attach to different LN domains suggested the presence of more than one LN receptor. These multiple LN receptors were shown to be beta 1 integrin heterodimers because antibodies to the integrin beta 1 subunit inhibited attachment of JAR cells to LN and its three fragments. To identify the individual integrin alpha/beta 1 heterodimers that mediate interactions with these LN domains, mAbs specific for individual beta 1 heterodimers in human cells were used to study JAR cell interactions with LN and its fragments. An anti-alpha 6/beta 1-specific mAb, GoH3, virtually eliminated cell attachment to E8 and partially inhibited attachment to E1 and intact LN. Thus the major alpha 6/beta 1 attachment domain is present in fragment E8. An alpha 1/beta 1-specific mAb (S2G3) strongly inhibited cell attachment to collagen IV and partially inhibited JAR attachment to LN fragment E1. Thus, the alpha 1/beta 1 heterodimer is a dual receptor for collagen IV and LN, interacting with LN at a site in fragment E1. In combination, the anti-alpha 1- and anti-alpha 6-specific antibodies completely inhibited JAR cell attachment to LN and fragment E1. Thus, the alpha 1/beta 1 and alpha 6/beta 1 integrin heterodimers each function as LN receptors and act together to mediate the interactions of human JAR choriocarcinoma cells with LN

Oxygen Phonon Branches in Detwinned YBa2Cu3O7

We report results of inelastic neutron scattering measurements of phonon dispersions on a detwinned sample of YBaCu3O7 and compare them with model calculations. Plane oxygen bond stretching phonon branches disperse steeply downwards from the zone center in both the a and the b direction indicating a strong electron-phonon coupling. Half way to the zone boundary, the phonon peaks become ill-defined but we see no need to invoke unit cell doubling or charge stripe formation: lattice dynamical shell model calculations predict such behavior as a result of branch anticrossings. There were no observable superconductivity-related temperature effects on selected plane oxygen bond stretching modes measured on a twinned sample.Comment: 5 pages, 3 figures, To appear in Journal of Low Temperature Physics (Proceedings of MOS2002; Revised version (1) with many changes throughout the tex