The role of regulatory mechanisms for control of plant diseases and food security — case studies from potato production in Britain

Being aware of the potentially devastating impacts of plant diseases on food security, governments have designed and employ plant health legislation to prevent or inhibit the worst impacts. The development of such policies in Britain, and latterly in Europe, can be closely linked to disease events that have occurred in the potato sector. We analyse early and current examples of policies governing potato diseases in Britain to identify the decision processes leading to the implementation of such phytosanitary policies and how they have evolved over time and in response to different disease threats. Reasons for developing and implementing phytosanitary policies include the desire to prevent pathogens being introduced (entering and establishing in a new area), the protection of export markets, and the lack of effective control measures. Circumstances in which regulatory policies would not be appropriate could include situations where a disease is already widely distributed, unacceptable costs, lack of exclusion measures, or difficulties of disease diagnosis. We conclude that in general, government policies have worked well in protecting British potato growing over the last one hundred years, despite of the failures of some of the policies discussed here. They have also contributed much to the development of plant health policies for other crops. Voluntary grower initiatives are a new mechanism complementing existing formal policies with an additional level of security that allows individual growers to take on additional responsibility rather than relying entirely on government legislation

Jurassic Redbeds of the Connecticut Valley: (1) Brownstones of the Portland Formation; and (2) Playa-Playa Lake-Oligomictic Lake Model for Parts of the East Berlin, Shuttle Meadow, and Portland Formations

Guidebook for field trips in Connecticut and south central Massachusetts: New England Intercollegiate Geological Conference 74th annual meeting, University of Connecticut, Storrs Connecticut , October 2 and 3, 1982: Trip M-

How global biodiversity hotspots may go unrecognized: Lessons from the North American Coastal Plain

© 2014 John Wiley & Sons Ltd. Biodiversity hotspots are conservation priorities. We identify the North American Coastal Plain (NACP) as a global hotspot based on the classic definition, a region with \u3e 1500 endemic plant species and \u3e 70% habitat loss. This region has been bypassed in prior designations due to misconceptions and myths about its ecology and history. These fallacies include: (1) young age of the NACP, climatic instability over time and submergence during high sea-level stands; (2) climatic and environmental homogeneity; (3) closed forest as the climax vegetation; and (4) fire regimes that are mostly anthropogenic. We show that the NACP is older and more climatically stable than usually assumed, spatially heterogeneous and extremely rich in species and endemics for its range of latitude, especially within pine savannas and other mostly herbaceous and fire-dependent communities. We suspect systematic biases and misconceptions, in addition to missing information, obscure the existence of similarly biologically significant regions world-wide. Potential solutions to this problem include (1) increased field biological surveys and taxonomic determinations, especially within grassy biomes and regions with low soil fertility, which tend to have much overlooked biodiversity; (2) more research on the climatic refugium role of hotspots, given that regions of high endemism often coincide with regions with low velocity of climate change; (3) in low-lying coastal regions, consideration of the heterogeneity in land area generated by historically fluctuating sea levels, which likely enhanced opportunities for evolution of endemic species; and (4) immediate actions to establish new protected areas and implement science-based management to restore evolutionary environmental conditions in newly recognized hotspots

Spectroscopy and excited-state g factors in weakly collective Cd 111: Confronting collective and microscopic models

Background: The even cadmium isotopes near the neutron midshell have long been considered among the best examples of vibrational nuclei. However, the vibrational nature of these nuclei has been questioned based on E2 transition rates that are not consistent with vibrational excitations. In the neighboring odd-mass nuclei, the g factors of the low-excitation collective states have been shown to be more consistent with a deformed rotational core than a vibrational core. Moving beyond the comparison of vibrational versus rotational models, recent advances in computational power have made shell-model calculations feasible for Cd isotopes. These calculations may give insights into the emergence and nature of collectivity in the Cd isotopes.This research was supported in part by the Australian Research Council Grants No. DP120101417, No. DP130104176, No. DP140102986, No. DP140103317, No. DP170101673, and No. LE150100064. B.J.C., A.A., J.T.H.D., M.S.M.G., and T.J.G. acknowledge support of the Australian Government Research Training Program. Support for the ANU Heavy Ion Accelerator Facility operations through the Australian National Collaborative Research Infrastructure Strategy (NCRIS) program is acknowledged

On the Influence of Alloy Composition on the Additive Manufacturability of Ni-Based Superalloys

The susceptibility of nickel-based superalloys to processing-induced crack formation during laser powder-bed additive manufacturing is studied. Twelve different alloys—some of existing (heritage) type but also other newly-designed ones—are considered. A strong inter-dependence of alloy composition and processability is demonstrated. Stereological procedures are developed to enable the two dominant defect types found—solidification cracks and solid-state ductility dip cracks—to be distinguished and quantified. Differential scanning calorimetry, creep stress relaxation tests at 1000 °C and measurements of tensile ductility at 800 °C are used to interpret the effects of alloy composition. A model for solid-state cracking is proposed, based on an incapacity to relax the thermal stress arising from constrained differential thermal contraction; its development is supported by experimental measurements using a constrained bar cooling test. A modified solidification cracking criterion is proposed based upon solidification range but including also a contribution from the stress relaxation effect. This work provides fundamental insights into the role of composition on the additive manufacturability of these materials

E-cadherin can limit the transforming properties of activating β-catenin mutations

Wnt pathway deregulation is a common characteristic of many cancers. But only Colorectal Cancer predominantly harbours mutations in APC, whereas other cancer types (hepatocellular carcinoma, solid pseudopapillary tumours of pancreas) have activating mutations in β-catenin (CTNNB1). We have compared the dynamics and the potency of β-catenin mutations in vivo. Within the murine small intestine (SI), an activating mutation of β-catenin took much longer to achieve a Wnt deregulation and acquire a crypt-progenitor-cell (CPC) phenotype than Apc or Gsk3 loss. Within the colon, a single activating mutation of β-catenin was unable to drive Wnt deregulation or induce the CPC phenotype. This ability of β-catenin mutation to differentially transform the SI versus the colon correlated with significantly higher expression of the β-catenin binding partner E-cadherin. This increased expression is associated with a higher number of E-cadherin:β-catenin complexes at the membrane. Reduction of E-cadherin synergised with an activating mutation of β-catenin so there was now a rapid CPC phenotype within the colon and SI. Thus there is a threshold of β-catenin that is required to drive transformation and E-cadherin can act as a buffer to prevent β-catenin accumulation

A Compensatory Mutation Provides Resistance to Disparate HIV Fusion Inhibitor Peptides and Enhances Membrane Fusion

Fusion inhibitors are a class of antiretroviral drugs used to prevent entry of HIV into host cells. Many of the fusion inhibitors being developed, including the drug enfuvirtide, are peptides designed to competitively inhibit the viral fusion protein gp41. With the emergence of drug resistance, there is an increased need for effective and unique alternatives within this class of antivirals. One such alternative is a class of cyclic, cationic, antimicrobial peptides known as θ-defensins, which are produced by many non-human primates and exhibit broad-spectrum antiviral and antibacterial activity. Currently, the θ-defensin analog RC-101 is being developed as a microbicide due to its specific antiviral activity, lack of toxicity to cells and tissues, and safety in animals. Understanding potential RC-101 resistance, and how resistance to other fusion inhibitors affects RC-101 susceptibility, is critical for future development. In previous studies, we identified a mutant, R5-tropic virus that had evolved partial resistance to RC-101 during in vitro selection. Here, we report that a secondary mutation in gp41 was found to restore replicative fitness, membrane fusion, and the rate of viral entry, which were compromised by an initial mutation providing partial RC-101 resistance. Interestingly, we show that RC-101 is effective against two enfuvirtide-resistant mutants, demonstrating the clinical importance of RC-101 as a unique fusion inhibitor. These findings both expand our understanding of HIV drug-resistance to diverse peptide fusion inhibitors and emphasize the significance of compensatory gp41 mutations. © 2013 Wood et al

Pharmacologic and Clinical Considerations of Nalmefene, a Long Duration Opioid Antagonist, in Opioid Overdose

Opioid use disorder is a well-established and growing problem in the United States. It is responsible for both psychosocial and physical damage to the affected individuals with a significant mortality rate. Given both the medical and non-medical consequences of this epidemic, it is important to understand the current treatments and approaches to opioid use disorder and acute opioid overdose. Naloxone is a competitive mu-opioid receptor antagonist that is used for the reversal of opioid intoxication. When given intravenously, naloxone has an onset of action of approximately 2 min with a duration of action of 60–90 min. Related to its empirical dosing and short duration of action, frequent monitoring of the patient is required so that the effects of opioid toxicity, namely respiratory depression, do not return to wreak havoc. Nalmefene is a pure opioid antagonist structurally similar to naltrexone that can serve as an alternative antidote for reversing respiratory depression associated with acute opioid overdose. Nalmefene is also known as 6-methylene naltrexone. Its main features of interest are its prolonged duration of action that surpasses most opioids and its ability to serve as an antidote for acute opioid overdose. This can be pivotal in reducing healthcare costs, increasing patient satisfaction, and redistributing the time that healthcare staff spend monitoring opioid overdose patients given naloxone

A survey of statistics in three UK general practice journal

Background Many medical specialities have reviewed the statistical content of their journals. To our knowledge this has not been done in general practice. Given the main role of a general practitioner as a diagnostician we thought it would be of interest to see whether the statistical methods reported reflect the diagnostic process. Methods Hand search of three UK journals of general practice namely the British Medical Journal (general practice section), British Journal of General Practice and Family Practice over a one-year period (1 January to 31 December 2000). Results A wide variety of statistical techniques were used. The most common methods included t-tests and Chi-squared tests. There were few articles reporting likelihood ratios and other useful diagnostic methods. There was evidence that the journals with the more thorough statistical review process reported a more complex and wider variety of statistical techniques. Conclusions The BMJ had a wider range and greater diversity of statistical methods than the other two journals. However, in all three journals there was a dearth of papers reflecting the diagnostic process. Across all three journals there were relatively few papers describing randomised controlled trials thus recognising the difficulty of implementing this design in general practice