A bioassay technique for the pheromone emitted by Amblyomma hebraeum males

Attachment of Amblyomma hebraeum females, either around feeding males or in areas in which extracts of fed males had previously been placed, was studied. The percentage attachment of female ticks around feeding males was significantly higher than that inside extract-treated areas. It was also found that the percentage attachment around the males increases with time, but there is little or no increase in attachment with time inside the treated areas. The amount of pheromone released by 1 feeding male was enough to stimulate attachment of females.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format

The relative resistance of six cattle breeds to the tick Boophilus decoloratus in South Africa

Adult females of the tick Boophilus decoloratus were removed from 6 breeds of cattle on 2 farms in the Northern Transvaal. Highest numbers of female ticks were collected from Simmentaler, followed by Santa Gertrudis, Bonsmara, Afrikaner, Brahman and Nguni. Resistance levels of each breed to B. decoloratus was positively correlated with the amount of Bos indicus genes in the breed, with the exception of Nguni, which is a sanga type but not pure B. indicus.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.Department of Agricultural Development. Department of Development Aid. CSIR, Pretoria.mn201

On-site secretory vesicle delivery drives filamentous growth in the fungal pathogen Candida albicans

International audienceCandida albicans is an opportunistic fungal pathogen that colonises the skin as well as genital and intestinal mucosa of most healthy individuals. The ability of C. albicans to switch between different morphological states, for example, from an ellipsoid yeast form to a highly polarised, hyphal form, contributes to its success as a pathogen. In highly polarised tip-growing cells such as neurons, pollen tubes, and filamentous fungi, delivery of membrane and cargo to the filament apex is achieved by long-range delivery of secretory vesicles tethered to motors moving along cytoskeletal cables that extend towards the growing tip. To investigate whether such a mechanism is also critical for C. albicans filamentous growth, we studied the dynamics and organisation of the C. albicans secretory pathway using live cell imaging and three-dimensional electron microscopy. We demonstrate that the secretory pathway is organised in distinct domains, including endoplasmic reticulum membrane sheets that extend along the length of the hyphal filament, a sub-apical zone exhibiting distinct membrane structures and dynamics and a Spitzenkörper comprised of uniformly sized secretory vesicles. Our results indicate that the organisation of the secretory pathway in C. albicans likely facilitates short-range "on-site" secretory vesicle delivery, in contrast to filamentous fungi and many highly polarised cells

Structural studies demonstrating a bacteriophage-like replication cycle of the eukaryote-infecting \u3ci\u3eParamecium bursaria\u3c/i\u3e chlorella virus-1

A fundamental stage in viral infection is the internalization of viral genomes in host cells. Although extensively studied, the mechanisms and factors responsible for the genome internalization process remain poorly understood. Here we report our observations, derived from diverse imaging methods on genome internalization of the large dsDNA Paramecium bursaria chlorella virus-1 (PBCV-1). Our studies reveal that early infection stages of this eukaryotic- infecting virus occurs by a bacteriophage-like pathway, whereby PBCV-1 generates a hole in the host cell wall and ejects its dsDNA genome in a linear, base-pair-by-base-pair process, through a membrane tunnel generated by the fusion of the virus internal membrane with the host membrane. Furthermore, our results imply that PBCV-1 DNA condensation that occurs shortly after infection probably plays a role in genome internalization, as hypothesized for the infection of some bacteriophages. The subsequent perforation of the host photosynthetic membranes presumably enables trafficking of viral genomes towards host nuclei. Previous studies established that at late infection stages PBCV-1 generates cytoplasmic organelles, termed viral factories, where viral assembly takes place, a feature characteristic of many large dsDNA viruses that infect eukaryotic organisms. PBCV-1 thus appears to combine a bacteriophage-like mechanism during early infection stages with a eukaryotic-like infection pathway in its late replication cycle. Author summary -- Although extensively studied, the mechanisms responsible for internalization of viral genomes into their host cells remain unclear. A particularly interesting case of genome release and internalization is provided by the large Paramecium bursaria chlorella virus-1 (PBCV-1), which infects unicellular eukaryotic photosynthetic chlorella cells. In order to release its long dsDNA genome and to enable its translocation to the host nucleus, PBCV-1 must overcome multiple hurdles, including a thick host cell wall and multilayered chloroplast membranes that surround the host cytoplasm. Our observations indicate that these obstacles are dealt with perforations of the host wall, the host cellular membrane, and the host photosynthetic membranes by viral-encoded proteins. Furthermore, our results highlight a bacteriophage-like nature of early PBCV-1 infection stages, thus implying that this virus uniquely combines bacteriophage-like and eukaryotic-like pathways to accomplish its replication cycle

Resistance of Brahman and Simmentaler cattle to Southern African ticks

Field collections of ticks from two breeds of cattle in the North Western Transvaal showed the common tick species to be Rhipicephalus appendiculatus, Amblyomma hebraeum, Rhipicephalus evertsi evertsi and Hyalomma marginatum rufipes. The number of these ticks was higher on Simmentaler than on Brahman cattle. Correlation was found between the number of ticks on the cattle and their serum gamma globulin levels indicating an increase in the production of antibodies. Similar correlation was also found between the number of ticks on the cattle and the number of eosinophils in their blood. Resistance was probably acquired by the hosts and, it appears, to a higher degree in Brahman than in Simmentaler.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.Veterinary Research Institute, Onderstepoort. CSIR, Pretoria.mn201

Virus–host interactions: insights from the replication cycle of the large \u3ci\u3eParamecium bursaria\u3c/i\u3e chlorella virus

The increasing interest in cytoplasmic factories generated by eukaryotic-infecting viruses stems from the realization that these highly ordered assemblies may contribute fundamental novel insights to the functional significance of order in cellular biology. Here, we report the formation process and structural features of the cytoplasmic factories of the large dsDNA virus Paramecium bursaria chlorella virus 1 (PBCV-1). By combining diverse imaging techniques, including scanning transmission electron microscopy tomography and focused ion beam technologies, we show that the architecture and mode of formation of PBCV-1 factories are significantly different from those generated by their evolutionary relatives Vaccinia and Mimivirus. Specifically, PBCV-1 factories consist of a network of single membrane bilayers acting as capsid templates in the central region, and viral genomes spread throughout the host cytoplasm but excluded from the membranecontaining sites. In sharp contrast, factories generated by Mimivirus have viral genomes in their core, with membrane biogenesis region located at their periphery. Yet, all viral factories appear to share structural features that are essential for their function. In addition, our studies support the notion that PBCV-1 infection, which was recently reported to result in significant pathological outcomes in humans andmice, proceeds througha bacteriophage -like infection pathway

Statistical Mechanics of Structural Fluctuations

The theory of mesoscopic fluctuations is applied to inhomogeneous solids consisting of chaotically distributed regions with different crystalline structure. This approach makes it possible to describe statistical properties of such mixture by constructing a renormalized Hamiltonian. The relative volumes occupied by each of the coexisting structures define the corresponding geometric probabilities. In the case of a frozen heterophase system these probabilities should be given a priori. And in the case of a thermal heterophase mixture the structural probabilities are to be defined self-consistently by minimizing a thermodynamical potential. This permits to find the temperature behavior of the probabilities which is especially important near the points of structural phase transitions. The presense of these structural fluctuations yields a softening of a crystal and a decrease of the effective Debye temperature. These effects can be directly seen by nuclear gamma resonance since the occurrence of structural fluctuations is accompanied by a noticeable sagging of the M\"ossbauer factor at the point of structural phase transition. The structural fluctuations also lead to the attenuation of sound and increase of isothermic compressibility.Comment: 1 file, 18 pages, RevTex, no figure

Defect-Free Carbon Nanotube Coils

Carbon nanotubes are promising building blocks for various nanoelectronic components. A highly desirable geometry for such applications is a coil. However, coiled nanotube structures reported so far were inherently defective or had no free ends accessible for contacting. Here we demonstrate the spontaneous self-coiling of single-wall carbon nanotubes into defect-free coils of up to more than 70 turns with identical diameter and chirality, and free ends. We characterize the structure, formation mechanism, and electrical properties of these coils by different microscopies, molecular dynamics simulations, Raman spectroscopy, and electrical and magnetic measurements. The coils are highly conductive, as expected for defect-free carbon nanotubes, but adjacent nanotube segments in the coil are more highly coupled than in regular bundles of single-wall carbon nanotubes, owing to their perfect crystal momentum matching, which enables tunneling between the turns. Although this behavior does not yet enable the performance of these nanotube coils as inductive devices, it does point a clear path for their realization. Hence, this study represents a major step toward the production of many different nanotube coil devices, including inductors, electromagnets, transformers, and dynamos

Asymmetric Misfit Nanotubes: Chemical Affinity Outwits the Entropy at High-Temperature Solid-State Reactions

Asymmetric two-dimensional (2D) structures (often named Janus), like SeMoS and their nanotubes, have tremendous scope in material chemistry, nanophotonics, and nanoelectronics due to a lack of inversion symmetry and time-reversal symmetry. The synthesis of these structures is fundamentally difficult owing to the entropy-driven randomized distribution of chalcogens. Indeed, no Janus nanotubes were experimentally prepared, so far. Serendipitously, a family of asymmetric misfit layer superstructures (tubes and flakes), including LaX-TaX2 (where X = S/Se), were synthesized by high-temperature chemical vapor transport reaction in which the Se binds exclusively to the Ta atoms and La binds to S atoms rather than the anticipated random distribution.With increasing Se concentration, the LaS-TaX2 misfit structure gradually transformed into a new LaS-TaSe2-TaSe2 superstructure. No misfit structures were found for xSe = 1. These counterintuitive results shed light on the chemical selectivity and stability of misfit compounds and 2D alloys, in general. The lack of inversion symmetry in these asymmetric compounds induces very large local electrical dipoles. The loss of inversion and time-reversal symmetries in the chiral nanotubes offers intriguing physical observations and applications. © 2021 National Academy of Sciences. All rights reserved.ACKNOWLEDGMENTS. We thank Dr. Iddo Pinkas for the help with the Raman measurements. The support of the Israel Science Foundation (Grant 7130970101), Irving and Cherna Moskowitz Center for Nano and Bio-Nano Imaging, the Perlman Family Foundation, and the Kimmel Center for Nanoscale Science (Grant 43535000350000) are greatly acknowledged. Part of the TEM studies were conducted at the Laboratorio de Microscopias Avanzadas, Universidad de Zaragoza, Spain. S.H. and R.A. acknowledge funding by German Research Foundation (Deutsche Forschungsgemeinschaft project He 7675/1-1), by the Spanish Ministerio de Ciencia e Innovacion (PID2019-104739GB-100/AEI/ 10.13039/501100011033), and Government of Aragon (project DGA E13-20R [Fondo Europeo de Desarrollo Regional, European Union]). R.A. gratefully acknowledges the support from the European Union H2020 programs “ESTEEM3” (Grant 823717) and Graphene Flagship (881603)

Potential role of ticks as vectors of bluetongue virus

When the first outbreak of bluetongue virus serotype 8 (BTV8) was recorded in North-West Europe in August 2006 and renewed outbreaks occurred in the summer of 2007 and again in 2008, the question was raised how the virus survived the winter. Since most adult Culicoides vector midges are assumed not to survive the northern European winter, and transovarial transmission in Culicoides is not recorded, we examined the potential vector role of ixodid and argasid ticks for bluetongue virus. Four species of ixodid ticks (Ixodes ricinus, Ixodes hexagonus, Dermacentor reticulatus and Rhipicephalus bursa) and one soft tick species, Ornithodoros savignyi, ingested BTV8-containing blood either through capillary feeding or by feeding on artificial membranes. The virus was taken up by the ticks and was found to pass through the gut barrier and spread via the haemolymph into the salivary glands, ovaries and testes, as demonstrated by real-time reverse transcriptase PCR (PCR-test). BTV8 was detected in various tissues of ixodid ticks for up to 21 days post feeding and in Ornithodoros ticks for up to 26 days. It was found after moulting in adult Ixodes hexagonus and was also able to pass through the ovaries into the eggs of an Ornithodoros savignyi tick. This study demonstrates that ticks can become infected with bluetongue virus serotype 8. The transstadial passage in hard ticks and transovarial passage in soft ticks suggest that ticks have potential vectorial capacity for bluetongue virus. Further studies are required to investigate transmission from infected ticks to domestic livestock. This route of transmission could provide an additional clue in the unresolved mystery of the epidemiology of Bluetongue in Europe by considering ticks as a potential overwintering mechanism for bluetongue virus