9,629 research outputs found
Prediction of Uranium Transport in an Aquifer at a Proposed Uranium In Situ Recovery Site: Geochemical Modeling as a Decision-Making Tool
Roll fronts are some of the most important uranium deposits and are quite common in the United States. Generally, a roll front has an oxidized zone and a reduced zone, the latter being the zone of high mineralization and a target for in situ recovery (ISR) mining. The challenge remains the gathering of information to enable making informed decisions regarding post-mining groundwater quality. In this study, potential uncertainties in uranium sorption on iron oxyhydroxides or hydrous ferric oxides (HFO) following mining were assessed, as these oxidized zones create a greater risk for future uranium transport than fully reduced zones. Using two different geochemical databases, uncertainties in predicting uranium sorption on HFO based on a post-recovery restoration scenario were studied. The scenario was assessed using one-dimensional PHREEQC geochemical modeling simulations with respect to: uranium, oxygen, carbon dioxide, and iron hydroxide concentrations. The results of the simulations showed that uranium concentrations in solution are likely to be controlled by the amount of HFO available for sorption and the concentration of uranium-carbonate complexes formed in the solution. The presence of calcium, through the dissolution of calcite, was found to reduce the adsorption of uranium onto HFO as the resulting uranium-calcium-carbonate complexes are quite soluble. Overall, the simulations provide a procedure for predicting down-gradient uranium concentrations based on ultimate restoration levels at uranium ISR sites. This is important for risk assessment, regulatory enforcement, and decision making
Chiral Quantum Walks
Given its importance to many other areas of physics, from condensed matter
physics to thermodynamics, time-reversal symmetry has had relatively little
influence on quantum information science. Here we develop a network-based
picture of time-reversal theory, classifying Hamiltonians and quantum circuits
as time-symmetric or not in terms of the elements and geometries of their
underlying networks. Many of the typical circuits of quantum information
science are found to exhibit time-asymmetry. Moreover, we show that
time-asymmetry in circuits can be controlled using local gates only, and can
simulate time-asymmetry in Hamiltonian evolution. We experimentally implement a
fundamental example in which controlled time-reversal asymmetry in a
palindromic quantum circuit leads to near-perfect transport. Our results pave
the way for using time-symmetry breaking to control coherent transport, and
imply that time-asymmetry represents an omnipresent yet poorly understood
effect in quantum information science.Comment: 9 pages, 4 figures, REVTeX 4.1 - published versio
The structure of a resuscitation-promoting factor domain from Mycobacterium tuberculosis shows homology to lysozymes
Resuscitation-promoting factor (RPF) proteins reactivate stationary-phase cultures of (G+C)-rich Gram-positive bacteria including the causative agent of tuberculosis, Mycobacterium tuberculosis. We report the solution structure of the RPF domain from M. tuberculosis Rv1009 (RpfB) solved by heteronuclear multidimensional NMR. Structural homology with various glycoside hydrolases suggested that RpfB cleaved oligosaccharides. Biochemical studies indicate that a conserved active site glutamate is important for resuscitation activity. These data, as well as the presence of a clear binding pocket for a large molecule, indicate that oligosaccharide cleavage is probably the signal for revival from dormancy
The molecular characterisation of Escherichia coli K1 isolated from neonatal nasogastric feeding tubes
Background: The most common cause of Gram-negative bacterial neonatal meningitis is E. coli K1. It has a mortality rate of 10–15%, and neurological sequelae in 30– 50% of cases. Infections can be attributable to nosocomial sources, however the pre-colonisation of enteral feeding tubes has not been considered as a specific risk factor. Methods: Thirty E. coli strains, which had been isolated in an earlier study, from the residual lumen liquid and biofilms of neonatal nasogastric feeding tubes were genotyped using pulsed-field gel electrophoresis, and 7-loci multilocus sequence typing. Potential pathogenicity and biofilm associated traits were determined using specific PCR probes, genome analysis, and in vitro tissue culture assays. Results: The E. coli strains clustered into five pulsotypes, which were genotyped as sequence types (ST) 95, 73, 127, 394 and 2076 (Achman scheme). The extra-intestinal pathogenic E. coli (ExPEC) phylogenetic group B2 ST95 serotype O1:K1:NM strains had been isolated over a 2 week period from 11 neonates who were on different feeding regimes. The E. coli K1 ST95 strains encoded for various virulence traits associated with neonatal meningitis and extracellular matrix formation. These strains attached and invaded intestinal, and both human and rat brain cell lines, and persisted for 48 h in U937 macrophages. E. coli STs 73, 394 and 2076 also persisted in macrophages and invaded Caco-2 and human brain cells, but only ST394 invaded rat brain cells. E. coli ST127 was notable as it did not invade any cell lines. Conclusions: Routes by which E. coli K1 can be disseminated within a neonatal intensive care unit are uncertain, however the colonisation of neonatal enteral feeding tubes may be one reservoir source which could constitute a serious health risk to neonates following ingestion
Transition Region Emission from Solar Flares during the Impulsive Phase
There are relatively few observations of UV emission during the impulsive
phases of solar flares, so the nature of that emission is poorly known. Photons
produced by solar flares can resonantly scatter off atoms and ions in the
corona. Based on off-limb measurements by SOHO/UVCS, we derive the O VI
1032 luminosities for 29 flares during the impulsive phase and the
Ly luminosities of 5 flares, and we compare them with X-ray
luminosities from GOES measurements. The upper transition region and lower
transition region luminosities of the events observed are comparable. They are
also comparable to the luminosity of the X-ray emitting gas at the beginning of
the flare, but after 10-15 minutes the X-ray luminosity usually dominates. In
some cases we can use Doppler dimming to estimate flow speeds of the O VI
emitting gas, and 5 events show speeds in the 40 to 80 range.
The O VI emission could originate in gas evaporating to fill the X-ray flare
loops, in heated chromospheric gas at the footpoints, or in heated prominence
material in the coronal mass ejection. All three sources may contribute in
different events or even in a single event, and the relative timing of UV and
X-ray brightness peaks, the flow speeds, and the total O VI luminosity favor
each source in one or more events.Comment: 18 pages, 8 figures, 3 table
A One Health Framework for the Evaluation of Rabies Control Programmes: A Case Study from Colombo City, Sri Lanka
<div><p>Background</p><p>One Health addresses complex challenges to promote the health of all species and the environment by integrating relevant sciences at systems level. Its application to zoonotic diseases is recommended, but few coherent frameworks exist that combine approaches from multiple disciplines. Rabies requires an interdisciplinary approach for effective and efficient management.</p><p>Methodology/Principal Findings</p><p>A framework is proposed to assess the value of rabies interventions holistically. The economic assessment compares additional monetary and non-monetary costs and benefits of an intervention taking into account epidemiological, animal welfare, societal impact and cost data. It is complemented by an ethical assessment. The framework is applied to Colombo City, Sri Lanka, where modified dog rabies intervention measures were implemented in 2007. The two options included for analysis were the control measures in place until 2006 (“baseline scenario”) and the new comprehensive intervention measures (“intervention”) for a four-year duration. Differences in control cost; monetary human health costs after exposure; Disability-Adjusted Life Years (DALYs) lost due to human rabies deaths and the psychological burden following a bite; negative impact on animal welfare; epidemiological indicators; social acceptance of dogs; and ethical considerations were estimated using a mixed method approach including primary and secondary data. Over the four years analysed, the intervention cost US $1.03 million more than the baseline scenario in 2011 prices (adjusted for inflation) and caused a reduction in dog rabies cases; 738 DALYs averted; an increase in acceptability among non-dog owners; a perception of positive changes in society including a decrease in the number of roaming dogs; and a net reduction in the impact on animal welfare from intermediate-high to low-intermediate.</p><p>Conclusions</p><p>The findings illustrate the multiple outcomes relevant to stakeholders and allow greater understanding of the value of the implemented rabies control measures, thereby providing a solid foundation for informed decision-making and sustainable control.</p></div
Possible Role of Horizontal Gene Transfer in the Colonization of Sea Ice by Algae
Diatoms and other algae not only survive, but thrive in sea ice. Among sea ice diatoms, all species examined so far produce ice-binding proteins (IBPs), whereas no such proteins are found in non-ice-associated diatoms, which strongly suggests that IBPs are essential for survival in ice. The restricted occurrence also raises the question of how the IBP genes were acquired. Proteins with similar sequences and ice-binding activities are produced by ice-associated bacteria, and so it has previously been speculated that the genes were acquired by horizontal transfer (HGT) from bacteria. Here we report several new IBP sequences from three types of ice algae, which together with previously determined sequences reveal a phylogeny that is completely incongruent with algal phylogeny, and that can be most easily explained by HGT. HGT is also supported by the finding that the closest matches to the algal IBP genes are all bacterial genes and that the algal IBP genes lack introns. We also describe a highly freeze-tolerant bacterium from the bottom layer of Antarctic sea ice that produces an IBP with 47% amino acid identity to a diatom IBP from the same layer, demonstrating at least an opportunity for gene transfer. Together, these results suggest that the success of diatoms and other algae in sea ice can be at least partly attributed to their acquisition of prokaryotic IBP genes
Opportunity rover localization and topographic mapping at the landing site of Meridiani Planum, Mars
This paper presents the results of Mars topographic mapping and lander and rover localization for the Opportunity rover at Meridiani Planum during the Mars Exploration Rover (MER) 2003 mission. By Sol 458, the Opportunity rover traversed a distance of 5.20 km. We localized the lander using two-way Doppler radio positioning and cartographic triangulation of craters visible in both orbital and ground images. Additional high-resolution orbital images were taken to verify the determined lander position. Visual odometry and bundle adjustment techniques were applied to overcome wheel slippages, azimuthal angle drift, and other navigation errors (as large as 21% within Eagle crater). In addition, orbit-to-ground image-based adjustment was applied to correct rover location errors where bundle adjustment was not applicable. We generated timely topographic products, including orthoimages, digital terrain models (DTMs), three-dimensional (3-D) crater models, and rover traverse maps. In particular, detailed 3-D terrain models of major features, such as Endurance crater, have been generated using multisite panoramic stereo images based on bundle adjustment and wide baseline stereo technique
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