A simple device to collect, store and study samples of two-dimensional spider webs

Sin resumen.Fil: Ramirez, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales; ArgentinaFil: Ravelo, Alexandra M.. University of Alaska Fairbanks; Estados UnidosFil: Lopardo, Lara. University of Greifswald. Zoologisches Institut und Museum,; Alemani

Benthos

Currently, > 4,000 Arctic macro- and megabenthic species are known, representing the majority of Arctic marine faunal diversity. This estimate is expected to increase. • Benthic invertebrates are food to shes, marine mammals, seabirds and humans, and are commercially harvested. • Traditional Knowledge (TK) emphasizes the link between the benthic species and their predators, such as walrus, and their signi cance to culture. • Decadal changes in benthos biodiversity are observed in some well-studied regions, such as the Barents Sea and Chukchi Sea. • Drivers related to climate-change such as warming, ice decline and acidification are affecting the benthic community on a pan-Arctic scale, while drivers such as trawling, river/glacier discharge and invasive species have signficant impact on regional or local scales. • Increasing numbers of species are moving into, or shifting, their distributions in Arctic waters. These species will outcompete, prey on or offer less nutritious value as prey for Arctic species. • Current monitoring efforts have focused on macro- and megabenthic species, but have been confined to the Chukchi Sea and the Barents Sea. Efforts are increasing in waters of Greenland, Iceland, the Canadian Arctic, and in the Norwegian Sea. All other Arctic Marine Areas are lacking long-term benthic monitoring. • As a first step towards an international collaborative monitoring framework, we recommend to develop a time- and cost-effective, long-term and standardized monitoring of megabenthic communities in all Arctic regions with regular annual groundfish assessment surveys. Expanding monitoring on micro-, meio- and macrobenthic groups is encouraged

Biogeography of epibenthic assemblages in the central Beaufort Sea

Benthic communities change drastically in both biomass and community structure with increasing water depth on a global scale, attributed to a combination of food supply, environmental drivers, as well as physiological and competitive capacities. In the Arctic, benthic biogeographic patterns are additionally thought to be a result of the region’s glaciation history. Here, we investigate gross epibenthic biomass and assemblage structure turnover with water mass from coastal to bathyal depths from 136 beam trawl samples collected in the Beaufort Sea. We test whether Pacific Boreal Arctic species have their core distribution in shelf water masses while Atlantic Boreal Arctic species have wider depth ranges. Gross biomass estimates differed statistically among water masses, with high values mostly under the influences of the Polar Mixed Layer and Arctic Halocline (outer shelf and upper slope, respectively). Stations in the Coastal Zone and Canada Basin Deep Water had the lowest biomass. Epibenthic assemblages also differed significantly among water masses, with high taxon richness in shelf water masses that decreased considerably with depth. Biomass of benthic taxa with Pacific Boreal Arctic affinity was essentially limited to the shelf, while Atlantic Boreal Arctic taxa occurred across a broad depth range, though their biomass increased in deeper water masses for mollusks and echinoderms, but not for decapods/isopods. Our results confirm earlier evidence of a strong Atlantic-Arctic deep-water connectivity reaching into the Pacific Arctic region and suggest new arrivals of species from the boreal Pacific are likely to settle on Pacific Arctic shelves, but are unlikely to invade continental slope and basin waters in the foreseeable future

Growth and production of the brittle stars Ophiura sarsii and Ophiocten sericeum (Echinodermata: Ophiuroidea)

Dense brittle star assemblages dominate vast areas of the Arctic marine shelves, making them key components of Arctic ecosystem. This study is the first to determine the population dynamics of the dominant shelf brittle star species, Ophiura sarsii and Ophiocten sericeum, through age determination, individual production and total turnover rate (P:B). In the summer of 2013, O. sarsii were collected in the northeastern Chukchi Sea (depth 35–65 m), while O. sericeum were collected in the central Beaufort Sea (depth 37–200 m). Maximum age was higher for O. sarsii than for O. sericeum (27 and 20 years, respectively); however, both species live longer than temperate region congeners. Growth curves for both species had similar initial fast growth, with an inflection period followed by a second phase of fast growth. Predation avoidance in addition to changes in the allocation of energy may be the mechanisms responsible for the observed age dependent growth rates. Individual production was higher for O. sarsii than for O. sericeum by nearly an order of magnitude throughout the size spectra. The distinct distribution pattern of the two species in the Alaskan Arctic may be determined by environmental characteristics such as system productivity. Both species had equally low turnover rates (0.2 and 0.1, respectively), similar to Antarctic species, but lower than temperate species. Such characteristics suggest that the dense brittle star assemblages that characterize the Arctic shelf system could have a recovery time from disturbance on the order of decades

Evolution of Silk Anchor Structure as the Joint Effect of Spinning Behavior and Spinneret Morphology

Spider web anchors are attachment structures composed of the bi-phasic glue-fiber secretion from the piriform silk glands. The mechanical performance of the anchors strongly correlates with the structural assembly of the silk lines, which makes spider silk anchors an ideal system to study the biomechanical function of extended phenotypes and its evolution. It was proposed that silk anchor function guided the evolution of spider web architectures, but its finestructural variation and whether its evolution was rather determined by changes of the shape of the spinneret tip or in the innate spinning choreography remained unresolved. Here, we comparatively studied the micro-structure of silk anchors across the spider tree of life, and set it in relation to spinneret morphology, spinning behavior and the ecology of the spider. We identified a number of apomorphies in the structure of silk anchors that may positively affect anchor function: (1) bundled dragline, (2) dragline envelope, and (3) dragline suspension ("bridge"). All these characters were apomorphic and evolved repeatedly in multiple lineages, supporting the notion that they are adaptive. The occurrence of these structural features can be explained with changes in the shape and mobility of the spinneret tip, the spinning behavior, or both. Spinneret shapes generally varied less than their fine-tuned movements, indicating that changes in construction behavior play a more important role in the evolution of silk anchor assembly. However, the morphology of the spinning apparatus is also a major constraint to the evolution of the spinning choreography. These results highlight the changes in behavior as the proximate and in morphology as the ultimate causes of extended phenotype evolution. Further, this research provides a roadmap for future bioprospecting research to design high-performance instant line anchors.Fil: Wolff, Jonas O.. Macquarie University; AustraliaFil: Michalik, Peter. ERNST MORITZ ARNDT UNIVERSITÄT GREIFSWALD (UG);Fil: Ravelo, Alexandra M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Herberstein, Mariella E.. Macquarie University; AustraliaFil: Ramirez, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentin

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field