39 research outputs found
Novedades taxonĂłmicas en especies sudamericanas de Gutierrezia (Asteraceae, Astereae)
779-785Novedades taxonĂłmicas en especies sudamericanas de Gutierrezia (Asteraceae, Astereae). Durante la revisiĂłn de las especies sudamericanas de Gutierrezia (Asteraceae, Astereae) se establecieron novedades taxonĂłmicas: (1) Dos nuevas variedades fueron descriptas, Gutierrezia mandonii var. anomala del noroeste de Argentina y Gutierrezia isernii var. nivea del centro y oeste de Argentina; y (2) la especie Gutierrezia pulviniformis se incluyĂł como un nuevo sinĂłnimo de Gutierrezia spathulata
Novedades taxonĂłmicas en especies sudamericanas de Gutierrezia (Asteraceae, Astereae)
779-785Novedades taxonĂłmicas en especies sudamericanas de Gutierrezia (Asteraceae, Astereae). Durante la revisiĂłn de las especies sudamericanas de Gutierrezia (Asteraceae, Astereae) se establecieron novedades taxonĂłmicas: (1) Dos nuevas variedades fueron descriptas, Gutierrezia mandonii var. anomala del noroeste de Argentina y Gutierrezia isernii var. nivea del centro y oeste de Argentina; y (2) la especie Gutierrezia pulviniformis se incluyĂł como un nuevo sinĂłnimo de Gutierrezia spathulata
Una nueva especie de Gutierrezia (Asteraceae, Astereae) de Argentina
Ratto, Francisco. Universidad de Buenos Aires. Facultad de AgronomĂa. Departamento de Recursos Naturales y Ambiente. CĂĄtedra de BotĂĄnica SistemĂĄtica. Buenos Aires, Argentina.Bartoli, Adolfo. Universidad de Buenos Aires. Facultad de AgronomĂa. Departamento de Recursos Naturales y Ambiente. CĂĄtedra de BotĂĄnica SistemĂĄtica. Buenos Aires, Argentina.e004, 5 p.Gutierrezia tortosae Ratto & Adr. Bartoli, a new species from Argentina, is described and illustrated. The new species resembles Gutierrezia mandonii (Sch. Bip.) Solbrig, in having xylopodium, similar shaped leaves, and yellow ray florets, but differs by having erect and rigid stems, erect leaves and smaller heads. A key to differentiate it from the allied species which inhabit in the northwest of Argentina is provided
Arquitectura del género Gutierrezia (Asteraceae) : Astereae, Solidagininae)
Gambino, Silvana. Universidad de Buenos Aires. Facultad de AgronomĂa. Departamento de Recursos Naturales y Ambiente. CĂĄtedra de BotĂĄnica SistemĂĄtica. Buenos Aires, Argentina.Ratto, Francisco. Universidad de Buenos Aires. Facultad de AgronomĂa. Departamento de Recursos Naturales y Ambiente. CĂĄtedra de BotĂĄnica SistemĂĄtica. Buenos Aires, Argentina.Bartoli, Carmen MarĂa Adriana. Universidad de Buenos Aires. Facultad de AgronomĂa. Departamento de Recursos Naturales y Ambiente. CĂĄtedra de BotĂĄnica SistemĂĄtica. Buenos Aires, Argentina.657-663An architectural study of the genus Gutierrezia Lag. was carried out, and seven different models were established. Differences in architecture between North American and South American species are pointed out. A key to differentiate the models is provided
Arquitectura del género Gutierrezia (Asteraceae) : Astereae, Solidagininae)
Gambino, Silvana. Universidad de Buenos Aires. Facultad de AgronomĂa. Departamento de Recursos Naturales y Ambiente. CĂĄtedra de BotĂĄnica SistemĂĄtica. Buenos Aires, Argentina.Ratto, Francisco. Universidad de Buenos Aires. Facultad de AgronomĂa. Departamento de Recursos Naturales y Ambiente. CĂĄtedra de BotĂĄnica SistemĂĄtica. Buenos Aires, Argentina.Bartoli, Carmen MarĂa Adriana. Universidad de Buenos Aires. Facultad de AgronomĂa. Departamento de Recursos Naturales y Ambiente. CĂĄtedra de BotĂĄnica SistemĂĄtica. Buenos Aires, Argentina.657-663An architectural study of the genus Gutierrezia Lag. was carried out, and seven different models were established. Differences in architecture between North American and South American species are pointed out. A key to differentiate the models is provided
ÎČ-NGF Stimulates Steroidogenic Enzyme and VEGFA Gene Expression, and Progesterone Secretion via ERK 1/2 Pathway in Primary Culture of Llama Granulosa Cells
The beta-nerve growth factor (ÎČ-NGF) from llama seminal plasma exerts ovulatory and luteotrophic effects following intramuscular or intrauterine infusion in llamas and alpacas. In this study, we investigate the in vitro effect of llama ÎČ-NGF on the expression of genes involved in angiogenesis and progesterone synthesis as well as progesterone release in preovulatory llama granulosa cells; we also determine whether these changes are mediated via the ERK1/2 signaling pathway. From adult female llamas, we collected granulosa cells from preovulatory follicles by transvaginal ultrasound-guided follicle aspiration; these cells were pooled and incubated. After 80% confluence, the cultured granulosa cells were treated with ÎČ-NGF,ÎČ-NGF plus the MAPK inhibitor U0126, or luteinizing hormone, and the abundance of angiogenic and steroidogenic enzyme mRNA transcripts were quantified after 10 and 20 h by RT-qPCR. We also quantified the progesterone concentration in the media after 48 h by radioimmunoassay. We found that application of ÎČ-NGF increases the abundance of mRNA transcripts of the vascular endothelial growth factor (VEGFA) and the steroidogenic enzymes cytochrome P450 side-chain cleavage (P450scc/CYP11A1), steroidogenic acute regulatory protein (STAR), and 3ÎČ-hydroxysteroid dehydrogenase (HSD3B1) at 10 and 20 h of treatment. Application of the MAPK inhibitor U0126 resulted in downregulation of the genes encoding these enzymes. ÎČ-NGF also enhanced progesterone synthesis, which was prevented by the prior application of the MAPK inhibitor U0126. Finally, western blot analysis confirmed that ÎČ-NGF activates the ERK1/2 signaling pathway. In conclusion, our results indicate that ÎČ-NGF exerts direct luteotropic effects on llama ovarian tissue via the ERK 1/2 pathway.Fil: Valderrama, Ximena. Instituto de Investigaciones Agropecuarias.; ChileFil: Ullo Leal, Cesar. Universidad Austral de Chile; ChileFil: Silva, Mauricio Erciario. Universidad Catolica de Temuco.; ChileFil: Goicochea, Jose. Universidad Nacional Valdizan; PerĂșFil: Apichela, Silvana Andrea. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - TucumĂĄn. Instituto Superior de Investigaciones BiolĂłgicas. Universidad Nacional de TucumĂĄn. Instituto Superior de Investigaciones BiolĂłgicas; ArgentinaFil: Argañaraz, Martin Eduardo. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - TucumĂĄn. Instituto Superior de Investigaciones BiolĂłgicas. Universidad Nacional de TucumĂĄn. Instituto Superior de Investigaciones BiolĂłgicas; ArgentinaFil: Sari, Luciana MarĂa. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - TucumĂĄn. Instituto Superior de Investigaciones BiolĂłgicas. Universidad Nacional de TucumĂĄn. Instituto Superior de Investigaciones BiolĂłgicas; ArgentinaFil: Paiva, Luis Ernesto. Universidad Austral de Chile; ChileFil: Ratto, Vicente Francisco. Universidad Austral de Chile; ChileFil: Ratto, Marcelo Hector. Universidad Austral de Chile; Chil
Effects of NGF Addition on Llama (Lama glama) Sperm Traits After Cooling
To provide new insights into the mechanisms through which seminal plasma proteins can protect sperm from damage caused during refrigeration, we evaluate the possibility that ÎČ-NGF can contribute to the improvement of sperm quality after cooling. First, ÎČ-NGF was detected in refrigerated sperm and compared with unrefrigerated sperm by western blotting of the proteins adsorbed by sperm, showing that native ÎČ-NGF is still present even 24 h after cooling only as an active form. Then, the effect of exogenous ÎČ-NGF on sperm quality after cooling was evaluated. A total of 12 ejaculates from male llamas (three ejaculates per male), were obtained by electro-ejaculation, diluted 4:1 with buffer Hepes-balanced salt solution and centrifuged at 800 Ă g for 8 min to remove the seminal plasma. Sperm were suspended in Tris-citrate-fructose-egg yolk diluent for a final concentration of 30 Ă106/ml and cooled at 5°C for 24 h. After refrigeration, the extended sperm were equilibrated for 5 min at 37°C and divided into the following subgroups: sperm samples without treatment (control) and sperm samples supplemented with exogenous human ÎČ-NGF (10, 100, and 500 ng/ml). At 5, 30, and 60 min of incubation sperm were evaluated for sperm viability (using eosin/nigrosin stain), sperm motility and vigor (observed under light microscopy), and mitochondrial activity (using the JC-1 fluorescent marker). Vigor data were analyzed with the nonparametric Kruskal-Wallis test. The rest of the variables were analyzed with a mixed models approach. Mean comparisons were performed using Fisher's LSD test with a confidence level of 95%. A principal components analysis was performed to analyze the relationships between variables. Treatment of 24 h cooled sperm with 10 or 100 ng/ml of human ÎČ-NGF increased the percentage of total motility and vigor (p < 0.05). Besides, an incubation time of 60 min would be adequate to improve sperm quality, since all variables are positively related. The significant improvement observed in the motility and vigor of post-refrigerated sperm suggests that supplementation with exogenous ÎČ-NGF may be profitable for the improvement of cooled llama sperm.Fil: Sari, Luciana MarĂa. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - TucumĂĄn. Instituto Superior de Investigaciones BiolĂłgicas. Universidad Nacional de TucumĂĄn. Instituto Superior de Investigaciones BiolĂłgicas; ArgentinaFil: Zampini, Renato. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - TucumĂĄn. Instituto Superior de Investigaciones BiolĂłgicas. Universidad Nacional de TucumĂĄn. Instituto Superior de Investigaciones BiolĂłgicas; ArgentinaFil: Gonzalez del Pino, Francisco. Universidad Nacional de TucumĂĄn; ArgentinaFil: Argañaraz, Martin Eduardo. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - TucumĂĄn. Instituto Superior de Investigaciones BiolĂłgicas. Universidad Nacional de TucumĂĄn. Instituto Superior de Investigaciones BiolĂłgicas; ArgentinaFil: Ratto, Marcelo Hector. Universidad Austral de Chile; ChileFil: Apichela, Silvana Andrea. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - TucumĂĄn. Instituto Superior de Investigaciones BiolĂłgicas. Universidad Nacional de TucumĂĄn. Instituto Superior de Investigaciones BiolĂłgicas; Argentin
Rock art and volcanology: input for a relative chronology of Las Papas engravings (dpto. Tinogasta, Catamarca)
La quebrada de Las Papas fue colmatada por flujos piroclĂĄsticos producto de la Ășltima erupciĂłn del Complejo VolcĂĄnico de Cerro Blanco (ca.4200 años AP) localizado en la Cordillera de San Buenaventura (4000 msnm). Cerca del pueblo de Las Papas se emplazan tres conjuntos de rocas grabadas a 2700 msnm y 20 km al sur del foco de la erupciĂłn. En los 23 bloques se identificaron 184 grabados fundamentalmente no-figurativos. Como presentan tanto pĂĄtinas claras como oscuras, no fue posible establecer una asociaciĂłn exclusiva entre una categorĂa de motivo y un tipo de pĂĄtina. En este trabajo presentamos un primer modelo de cĂĄlculos volumĂ©tricos para estimar la tasa de incisiĂłn en funciĂłn del desalojo del material piroclĂĄstico de la quebrada de Las Papas a lo largo de 4200 años, y estimar cuĂĄndo estuvieron los bloques disponibles para su intervenciĂłn. Para ello, articulamos el registro de campo, el anĂĄlisis de imĂĄgenes satelitales, el modelado y elaboraciĂłn de una paleo-topografĂa de curvas de nivel del terreno inmediatamente despuĂ©s de la erupciĂłn y la dataciĂłn de un entierro excavado en una terraza fluvial del rĂo Las Papas. De esta manera, pudimos estimar una cronologĂa relativa y establecer que las rocas soportes estuvieron disponibles para su intervenciĂłn visual a partir del año 900-1000 a.C. AdemĂĄs, el anĂĄlisis de las diferencias de pĂĄtina y la comparaciĂłn con el registro rupestre regional sugiere que la producciĂłn de los grabados fue discontinua en el tiempo y que un conjunto de imĂĄgenes no figurativas son las mĂĄs antiguas.Las Papas ravine was silted by pyroclastic flows the last eruption of the Cerro Blanco Volcanic Complex (ca.4200 years BP), located in the Cordillera de San Buenaventura (4000 masl). Three sets of engraved rocks are located near the town of Las Papas at 2700 masl and about 20 km south of the focus of eruption. In 23 blocks were identified 184 images with broad domain of non-figurative motifs. Since they have both light and dark patinas, it was not possible to establish an exclusive partnership between one category of motif and a kind of patina. This work presents a first model of volumetric calculations to estimate the rate of incision depending on the erosion of pyroclastic material of Las Papas ravine over 4200 years. To do this, we articulate field work, analysis of satellite images, modeling and development of the contour lines of paleo-topography immediately after the eruption, and dating of a burial excavated in a terrace of Las Papas river. We evaluate when each block was available for visual intervention, depending on their spatial location and altitude to estimate a relative chronology for the engraved media. In this way it was possible to estimate a relative chronology for the engraved blocks and to establish that they were available for their visual intervention from 900-1000 B.C. In addition, the analysis of the differences of patina and the comparison with the regional register suggests that the production of the engravings was discontinuous in time and that a set of non-figurative images are the ancient ones.Fil: Ratto, Norma Rosa. Universidad de Buenos Aires. Facultad de FilosofĂa y Letras. Museo EtnogrĂĄfico "Juan B. Ambrosetti"; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Rodriguez Gonzalez, Alejandro. Universidad de Las Palmas de Gran Canaria; EspañaFil: Basile, Mara Valeria. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Saavedra 15. Instituto de las Culturas. Universidad de Buenos Aires. Instituto de las Culturas; ArgentinaFil: Perez Torrado, Francisco. Universidad de Las Palmas de Gran Canaria; EspañaFil: Fernande Turiel, Jose Luis. Consejo Superior de Investigaciones CientĂficas; Españ
Outcomes from elective colorectal cancer surgery during the SARS-CoV-2 pandemic
This study aimed to describe the change in surgical practice and the impact of SARS-CoV-2 on mortality after surgical resection of colorectal cancer during the initial phases of the SARS-CoV-2 pandemic