Endocytosis and early endosome motility in filamentous fungi.

types: REVIEWOpen Access funded by Biotechnology and Biological Sciences Research CouncilHyphal growth of filamentous fungi requires microtubule-based long-distance motility of early endosomes. Since the discovery of this process in Ustilago maydis, our understanding of its molecular basis and biological function has greatly advanced. Studies in U. maydis and Aspergillus nidulans reveal a complex interplay of the motor proteins kinesin-3 and dynein, which co-operate to support bi-directional motion of early endosomes. Genetic screening has shed light on the molecular mechanisms underpinning motor regulation, revealing Hook protein as general motor adapters on early endosomes. Recently, fascinating insight into unexpected roles for endosome motility has emerged. This includes septin filament formation and cellular distribution of the machinery for protein translation.BBSR

Growth, domain structure, and atomic adsorption sites of hBN on the Ni(111) surface

One of the most important functionalities of the atomically thin insulator hexagonal boron nitride (hBN) is its ability to chemically and electronically decouple functional materials from highly reactive surfaces. It is therefore of utmost importance to uncover its structural properties on surfaces on an atomic and mesoscopic length scale. In this paper, we quantify the relative coverages of structurally different domains of a hBN layer on the Ni(111) surface using low-energy electron microscopy and the normal incidence x-ray standing wave technique. We find that hBN nucleates on defect sites of the Ni(111) surface and predominantly grows in two epitaxial domains that are rotated by 60∘ with respect to each other. The two domains reveal identical adsorption heights, indicating a similar chemical interaction strength with the Ni(111) surface. The different azimuthal orientations of these domains originate from different adsorption sites of N and B. We demonstrate that the majority (≈70%) of hBN domains exhibit a (N,B)=(top,fcc) adsorption site configuration while the minority (≈30%) show a (N,B)=(top,hcp) configuration. Our study hence underlines the crucial role of the atomic adsorption configuration in the mesoscopic domain structures of in situ fabricated two-dimensional materials on highly reactive surfac

An option for stranded renewables: electrolytic-hydrogen in future energy systems

Future energy systems will likely be challenged by large quantities of stranded renewable electricity that cannot be used in the conventional electrical grid. Using surplus electricity for electrolysis and thereby producing hydrogen is seen as a valuable solution functioning as an energy storage and transport medium and providing other sectors, such as transport or industry, with required feedstocks at the same time. In this study, we suggest using a set of assessment tools to highlight the quantitative potential, cost and environmental performance of electrolytic hydrogen production, transmission and storage. Our approach employs power sector modeling for Germany with three sequential elements: (i) a market model, (ii) power flow modeling, and (iii) re-dispatch modeling. The results were then used to identify suitable locations for large scale electrolysis plants. Electrolysis, large-scale gas storage, a transmission pipeline and other system components were scaled-up and the total cost was calculated. In a final step, we looked at greenhouse gas emissions as one of the major aspects regarding the environmental performance of the hydrogen delivered. Based on our analysis, annual hydrogen production rates of up to 189 kilotons have been determined for the state of Schleswig-Holstein, which exhibits the largest potential for utilizing surplus power from renewables. The economic analysis reveals a hydrogen cost of 3.63–5.81€ kg−1, including installations, for large-scale storage and transmission. If surplus power from renewables is used for hydrogen production, the total greenhouse gas emissions of hydrogen provision were determined to be up to 435 gCO2-eq. kg−1. Using grid electricity, this value increased to some 17 000 gCO2-eq. kg−1

Intramyocardially Transplanted Neonatal Cardiomyocytes (NCMs) Show Structural and Electrophysiological Maturation and Integration and Dose-Dependently Stabilize Function of Infarcted Rat Hearts

Cardiac cell replacement therapy is a promising therapy to improve cardiac function in heart failure. Persistence, structural and functional maturation, and integration of transplanted cardiomyocytes into recipients' hearts are crucial for a safe and efficient replacement of lost cells. We studied histology, electrophysiology, and quantity of intramyocardially transplanted rat neonatal cardiomyocytes (NCMs) and performed a detailed functional study with repeated invasive (pressure volume catheter) and noninvasive (echocardiography) analyses of infarcted female rat hearts including pharmacological stress before and 3 weeks after intramyocardial injection of 5 x 10(6) (low NCM) or 25 x 10(6) (high NCM) syngeneic male NCMs or medium as placebo (Ctrl). Quantitative real-time polymerase chain reaction (PCR) for Y-chromosome confirmed a fivefold higher persisting male cell number in high NCM versus low NCM after 3 weeks. Sharp electrode measurements within viable slices of recipient hearts demonstrated that transplanted NCMs integrate into host myocardium and mature to an almost adult phenotype, which might be facilitated through gap junctions between host myocardium and transplanted NCMs as indicated by connexin43 in histology. Ejection fraction of recipient hearts was severely impaired after ligation of left anterior descending (LAD; pressure volume catheter: 39.2 +/- 3.6%, echocardiography: 39.9 +/- 1.4%). Repeated analyses revealed a significant further decline within 3 weeks in Ctrl and a dose-dependent stabilization in cell-treated groups. Consistently, stabilized cardiac function/morphology in cell-treated groups was seen in stroke volume, cardiac output, ventricle length, and wall thickness. Our findings confirm that cardiac cell replacement is a promising therapy for ischemic heart disease since immature cardiomyocytes persist, integrate, and mature after intramyocardial transplantation, and they dose-dependently stabilize cardiac function after myocardial infarction