Structural characterization, antioxidant and anti-uropathogenic potential of biogenic silver nanoparticles using brown seaweed Turbinaria ornata

Alternative treatment strategies for urinary tract infections (UTIs) are becoming more necessary due to increasing drug resistance patterns in uropathogens. Nanoparticle-based therapeutics is emerging as a way to treat UTIs. In the present study, using Turbinaria ornata extract, silver nanoparticles (AgNPs) were synthesized, characterized, and their anti-uropathogenic activity was evaluated. The stability and formation of synthesized To-AgNPs were confirmed by UV-visible spectroscopy, FTIR, XRD, SEM, and DLS. An FTIR spectrum confirmed the presence of seaweed functional groups in To-AgNPs, a XRD analysis confirmed their crystalline nature, and SEM imaging confirmed their spherical nature with an average size of 73.98 nm with diameters ranging from 64.67 to 81.28 nm. This was confirmed by TEM results. DLS determined that the cumulant hydrodynamic diameter of To-AgNPs was 128.3 nm with a PdI of 0.313 and the zeta potential value were found to be –63.3 mV which indicates the To-AgNPs are negatively charged and more stable. DPPH assays were used to assess the antioxidant activity of biosynthesized To-AgNPs, while an agar well diffusion method was used to test the antibacterial activity against uropathogens, including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis, and Klebsiella pneumoniae. The To-AgNPs showed the highest susceptibility to S. aureus (15.75 ± 0.35 mm) and E. coli (15 ± 0.7 mm) with MIC values of 0.0625 and 0.125 mg/ml, respectively in macro broth dilution method and observed considerable membrane damage under CLSM and SEM. To-AgNPs displayed stronger antioxidant and antimicrobial activity, suggesting they may be developed as a new class of antimicrobial agents for treating UTIs

COVID-19: molecular pathophysiology, genetic evolution and prospective therapeutics—a review

The Covid-19 pandemic is highly contagious and has spread rapidly across the globe. To date there have been no specific treatment options available for this life-threatening disease. During this medical emergency, target-based drug repositioning/repurposing with a continuous monitoring and recording of results is an effective method for the treatment and drug discovery. This review summarizes the recent findings on COVID-19, its genomic organization, molecular evolution through phylogenetic analysis and has recapitulated the drug targets by analyzing the viral molecular machinery as drug targets and repurposing of most frequently used drugs worldwide and their therapeutic applications in COVID-19. Data from solidarity trials have shown that the treatment with Chloroquine, hydroxychloroquine and lopinavir-ritonavir had no effect in reducing the mortality rate and also had adverse side effects. Remdesivir, Favipiravir and Ribavirin might be a safer therapeutic option for COVID-19. Recent clinical trial has revealed that dexamethasone and convalescent plasma treatment can reduce mortality in patients with severe forms of COVID-19

Shallow Hydrothermal Vent Bacteria and Their Secondary Metabolites with a Particular Focus on <i>Bacillus</i>

Extreme environments are hostile for most organisms, but such habitats represent suitable settings to be inhabited by specialized microorganisms. A marine shallow-water hydrothermal vent field is located offshore in northeast Taiwan, near the shallow shore of the southeast of Kueishantao Island (121°55′ E, 24°50′ N). Research on extremophilic microorganisms makes use of the biotechnological potential associated with such microorganisms and their cellular products. With the notion that extremophiles are capable of surviving in extreme environments, it is assumed that their metabolites are adapted to function optimally under such conditions. As extremophiles, they need specific culture conditions, and only a fraction of species from the original samples are recovered in culture. We used different non-selective and selective media to isolate bacterial species associated with the hydrothermal vent crab Xenograpsus testudinatus and the sediments of its habitat. The highest number of colonies was obtained from Zobell marine agar plates with an overall number of 29 genetically distinct isolates. 16sRNA gene sequencing using the Sanger sequencing method revealed that most of the bacterial species belonged to the phylum Firmicutes and the class Bacilli. The present study indicates that hydrothermal vent bacteria and their secondary metabolites may play an important role for the reconstruction of the evolutionary history of the phylum Procaryota

ENVIRONMENT EDUCATION IN INDIA: CHALLENGES AND OPPORTUNITIES

It is now widely acknowledged that the impact of human activities on environment is significant and will have serious consequences for future generations. Environment education has a significant role in making the youth conscious about their environment, adopting green social behaviour and thus responding to the environment crises. Recognizing the importance and need of environment education, India has initiated several efforts including making the environment education compulsory at all level of education. Today when environmental conditions are changing adversely and all living beings are suffering from the negative consequences of environmental pollution and climate changes, there is a need to reorient the curriculum of environment education for making it more attractive and responsive to the local environmental issues. Moreover, related policies are also needed to be restructured for sensitizing the people, especially school and college level students about the issues related to environment management

L’action en force, les forces en action: Sociologie pragmatique des forces

Texte remanié de Thèse de doctorat : Sociologie : Metz : 2011. Titre de soutenance : L'action en force et les forces en action : sociologie de la force au travailInternational audienc

Whole genome sequence analysis and in-vitro probiotic characterization of Bacillus velezensis FCW2 from spontaneously fermented coconut water

In this study, the probiotic potential of B. velezensis FCW2, isolated from naturally fermented coconut water, was investigated by in vitro and genomic characterization. Our findings highlight key features of the bacterium which includes, antibacterial activity, high adhesive potential, aggregation capacity, production of nutrient secondary metabolites. In vivo safety assessment revealed no adverse effects on zebrafish. WGS data of B. velezensis FCW2 revealed a complete circular genome of 4,147,426 nucleotides and a GC content of 45.87%. We have identified 4059 coding sequence (CDS) genes that encode proteins involved in stress resistance, adhesion and micronutrient production. The genes responsible for producing secondary metabolites, exopolysaccharides, and other beneficial nutrients were identified. The KEGG and COG databases revealed that genes mainly involved amino acid metabolism, carbohydrate utilization, vitamin and cofactor metabolism, and biological adhesion. These findings suggest that B. velezensis FCW2 could be a putative probiotic in the development of fermented foods

Redistribution of riverine and rainfall freshwater by the Bay of Bengal circulation

International audienceWe use satellite-derived currents and a Lagrangian approach to investigate the redistribution of the precipitation minus evaporation (P-E) and river freshwater inputs into Bay of Bengal (BoB) by the oceanic circulation. We find a key role of Ekman transport in shaping the BoB freshwater distribution. Until September, the summer monsoon winds induce eastward Ekman transport, which maintains freshwater near its major rivers and rain sources in the northeastern BoB. The winter monsoon Ekman transport strongly contributes to the surface flow in many areas of the interior BoB. This ~ 0.15 m s−1 westward transport overcomes the weaker offshore transport by mesoscale motions and pushes a ~ 40/45% mixture of P-E and Ganges–Brahmaputra freshwater into the East Indian Coastal Current (EICC). In agreement with previous studies, we find that the EICC then transports Ganges–Brahmaputra freshwater southward, allowing the formation of a narrow freshwater tongue or “river in the sea” along the coast east of India in November. Ekman transport thus operates jointly with the EICC to allow the “river in the sea” formation. The EICC is nonetheless a “leaky pipe” as only ~ 22% of the Ganges–Brahmaputra, and ~ 9% of the P-E monsoonal freshwater inputs exit the BoB near Sri Lanka. The winter monsoon anticyclonic circulation in fact brings more rain freshwater from the equatorial Indian Ocean into the southeastern BoB than it exports freshwater through the EICC. As a result, the BoB circulation contributes to a net freshwater gain that amounts to 11% of the local rain and freshwater inputs

Genomic and metabolic properties of Staphylococcus gallinarum FCW1 MCC4687 isolated from naturally fermented coconut water towards GRAS assessment

Staphylococcus gallinarum FCW1 was isolated from naturally fermented coconut-water and identified by biochemical and molecular methods. Probiotic characterization and safety assessment were conducted through a series of in vitro tests. A high survival rate was observed when the strain was tested for resistance to bile, lysozyme, simulated gastric and intestinal fluid, phenol, and different temperature and salt concentrations. The strain showed antagonism against some pathogens, was susceptible to all antibiotics tested except penicillin, and showed no hemolytic and DNase activity. Hydrophobicity, autoaggregation, biofilm formation, and antioxidation tests indicated that the strain possessed a high adhesive and antioxidant ability. Enzymatic activity was used to evaluate the metabolic capacities of the strain. In-vivo experiment on zebrafish was performed to check its safety status. The whole-genome sequencing indicated that the genome contained 2,880,305 bp with a GC content of 33.23%. The genome annotation confirmed the presence of probiotic-associated genes and genes for oxalate degradation, sulfate reduction, acetate metabolism, and ammonium transport in the FCW1 strain, adding to the theory that this strain may be helpful in treating kidney stones. This study revealed that the strain FCW1 might be an excellent potential probiotic in developing fermented coconut beverages and treating and preventing kidney stone disease. [Abstract copyright: Copyright © 2023. Published by Elsevier B.V.