Potential Effects of Industrial Air Pollution and Wood-Product Supply and Demand, and Structure of the Wood-Products Industry, in Poland

This study aimed to determine potential changes in the production structure of the wood-processing industry up to 2020, resulting from unfavorable impact of industrial pollutants upon forests in Poland. The paper consists of four chapters. In the first section, forecasts of consumer demand for forest products, based on patterns of actual demand, are presented. The structure of industrial demand for wood assortments, and the degree to which it is met, are the topics of the second chapter. In the third chapter, we present forecasts of the possibilities of wood-raw-material consumption by industry with regard to the unfavorable impact of industrial pollution. The last chapter contains forecasts of production regarding foreseen changes in the structure of the wood-processing industry, taking into account qualitative changes in wood raw-material and expected changes in techniques and technology. Our results show that, up to 2020, negative effects of industrial pollutants on forests will have a significant influence on the degree of meeting consumer demands for wood products

Orbital and physical parameters of eclipsing binaries from the ASAS catalogue -- III. Two new low-mass systems with rapidly evolving spots

We present the results of our spectroscopic and photometric analysis of two newly discovered low-mass detached eclipsing binaries found in the All-Sky Automated Survey (ASAS) catalogue: ASAS J093814-0104.4 and ASAS J212954-5620.1. Using the GIRAFFE instrument on the 1.9-m Radcliffe telescope at SAAO and the UCLES spectrograph on the 3.9-m Anglo-Australian Telescope, we obtained high-resolution spectra of both objects and derived their radial velocities (RVs) at various orbital phases. The RVs of both objects were measured with the TODCOR technique using synthetic template spectra as references. We also obtained V and I band photometry using the 1.0-m Elizabeth telescope at SAAO and the 0.4-m PROMPT instruments located at the CTIO. The orbital and physical parameters of the systems were derived with PHOEBE and JKTEBOP codes. We compared our results with several sets of widely-used isochrones. Our multi-epoch photometric observations demonstrate that both objects show significant out-of-eclipse modulations, which vary in time. We believe that this effect is caused by stellar spots, which evolve on time scales of tens of days. For this reason, we constructed our models on the basis of photometric observations spanning short time scales (less than a month). Our modeling indicates that (1) ASAS-09 is a main sequence active system with nearly-twin components with masses of M1 = 0.771(33) Msun, M2 = 0.768(21) Msun and radii of R1 = 0.772(12) Rsun and R2 = 0.769(13) Rsun. (2) ASAS-21 is a main sequence active binary with component masses of M1 = 0.833(17) Msun, M2 = 0.703(13) Msun and radii of R1 = 0.845(12) Rsun and R2 = 0.718(17) Rsun. Both systems confirm the characteristic of active low-mass stars, for which the observed radii are larger and the temperatures lower than predicted by evolutionary models. Other parameters agree within errors with the models of main sequence stars.Comment: 15 pages, 7 figures, 7 tables, to appear in A&

TIMASSS : The IRAS16293-2422 Millimeter And Submillimeter Spectral Survey: Tentative Detection of Deuterated Methyl Formate (DCOOCH3)

High deuterium fractionation is observed in various types of environment such as prestellar cores, hot cores and hot corinos. It has proven to be an efficient probe to study the physical and chemical conditions of these environments. The study of the deuteration of different molecules helps us to understand their formation. This is especially interesting for complex molecules such as methanol and bigger molecules for which it may allow to differentiate between gas-phase and solid-state formation pathways. Methanol exhibits a high deuterium fractionation in hot corinos. Since CH3OH is thought to be a precursor of methyl formate we expect that deuterated methyl formate is produced in such environments. We have searched for the singly-deuterated isotopologue of methyl formate, DCOOCH3, in IRAS 16293-2422, a hot corino well-known for its high degree of methanol deuteration. We have used the IRAM/JCMT unbiased spectral survey of IRAS 16293-2422 which allows us to search for the DCOOCH3 rotational transitions within the survey spectral range (80-280 GHz, 328-366 GHz). The expected emission of deuterated methyl formate is modelled at LTE and compared with the observations.} We have tentatively detected DCOOCH3 in the protostar IRAS 16293-2422. We assign eight lines detected in the IRAM survey to DCOOCH3. Three of these lines are affected by blending problems and one line is affected by calibration uncertainties, nevertheless the LTE emission model is compatible with the observations. A simple LTE modelling of the two cores in IRAS 16293-2422, based on a previous interferometric study of HCOOCH3, allows us to estimate the amount of DCOOCH3 in IRAS 16293-2422. Adopting an excitation temperature of 100 K and a source size of 2\arcsec and 1\farcs5 for the A and B cores, respectively, we find that N(A,DCOOCH3) = N(B,DCOOCH3) ~ 6.10^14 /cm2. The derived deuterium fractionation is ~ 15%, consistent with values for other deuterated species in this source and much greater than that expected from the deuterium cosmic abundance. DCOOCH3, if its tentative detection is confirmed, should now be considered in theoretical models that study complex molecule formation and their deuteration mechanisms. Experimental work is also needed to investigate the different chemical routes leading to the formation of deuterated methyl formate

Evidence That a Lipolytic Enzyme—Hematopoietic-Specific Phospholipase C-β2—Promotes Mobilization of Hematopoietic Stem Cells by Decreasing Their Lipid Raft-Mediated Bone Marrow Retention and Increasing the Promobilizing Effects of Granulocytes

Hematopoietic stem/progenitor cells (HSPCs) reside in the bone marrow (BM) microenvironment and are retained there by the interaction of membrane lipid raft-associated receptors, such as the α-chemokine receptor CXCR4 and the α4β1-integrin (VLA-4, very late antigen 4 receptor) receptor, with their respective specific ligands, stromal-derived factor 1 and vascular cell adhesion molecule 1, expressed in BM stem cell niches. The integrity of the lipid rafts containing these receptors is maintained by the glycolipid glycosylphosphatidylinositol anchor (GPI-A). It has been reported that a cleavage fragment of the fifth component of the activated complement cascade, C5a, has an important role in mobilizing HSPCs into the peripheral blood (PB) by (i) inducing degranulation of BM-residing granulocytes and (ii) promoting their egress from the BM into the PB so that they permeabilize the endothelial barrier for subsequent egress of HSPCs. We report here that hematopoietic cell-specific phospholipase C-β2 (PLC-β2) has a crucial role in pharmacological mobilization of HSPCs. On the one hand, when released during degranulation of granulocytes, it digests GPI-A, thereby disrupting membrane lipid rafts and impairing retention of HSPCs in BM niches. On the other hand, it is an intracellular enzyme required for degranulation of granulocytes and their egress from BM. In support of this dual role, we demonstrate that PLC-β2-knockout mice are poor mobilizers and provide, for the first time, evidence for the involvement of this lipolytic enzyme in the mobilization of HSPCs

Very small embryonic-like stem cells (VSELs) represent a real challenge in stem cell biology : recent pros and cons in the midst of a lively debate

The concept that adult tissue, including bone marrow (BM), contains early-development cells with broader differentiation potential has again been recently challenged. In response, we would like to review the accumulated evidence from several independent laboratories that adult tissues, including BM, harbor a population of very rare stem cells that may cross germ layers in their differentiation potential. Thus, the BM stem cell compartment hierarchy needs to be revisited. These dormant, early-development cells that our group described as very small embryonic-like stem cells (VSELs) most likely overlap with similar populations of stem cells that have been identified in adult tissues by other investigators as the result of various experimental strategies and have been given various names. As reported, murine VSELs have some pluripotent stem cell characteristics. Moreover, they display several epiblast/germline markers that suggest their embryonic origin and developmental deposition in adult BM. Moreover, at the molecular level, changes in expression of parentally imprinted genes (for example, Igf2–H19) and resistance to insulin/insulin-like growth factor signaling (IIS) regulates their quiescent state in adult tissues. In several emergency situations related to organ damage, VSELs can be activated and mobilized into peripheral blood, and in appropriate animal models they contribute to tissue organ/regeneration. Interestingly, their number correlates with lifespan in mice, and they may also be involved in some malignancies. VSELs have been successfully isolated in several laboratories; however, some investigators experience problems with their isolation

Distinct RNA profiles in subpopulations of extracellular vesicles: apoptotic bodies, microvesicles and exosomes

Introduction: In recent years, there has been an exponential increase in the number of studies aiming to understand the biology of exosomes, as well as other extracellular vesicles. However, classification of membrane vesicles and the appropriate protocols for their isolation are still under intense discussion and investigation. When isolating vesicles, it is crucial to use systems that are able to separate them, to avoid cross-contamination. Method: EVs released from three different kinds of cell lines: HMC-1, TF-1 and BV-2 were isolated using two centrifugation-based protocols. In protocol 1, apoptotic bodies were collected at 2,000×g, followed by filtering the supernatant through 0.8 µm pores and pelleting of microvesicles at 12,200×g. In protocol 2, apoptotic bodies and microvesicles were collected together at 16,500×g, followed by filtering of the supernatant through 0.2 µm pores and pelleting of exosomes at 120,000×g. Extracellular vesicles were analyzed by transmission electron microscopy, flow cytometry and the RNA profiles were investigated using a Bioanalyzer®. Results: RNA profiles showed that ribosomal RNA was primary detectable in apoptotic bodies and smaller RNAs without prominent ribosomal RNA peaks in exosomes. In contrast, microvesicles contained little or no RNA except for microvesicles collected from TF-1 cell cultures. The different vesicle pellets showed highly different distribution of size, shape and electron density with typical apoptotic body, microvesicle and exosome characteristics when analyzed by transmission electron microscopy. Flow cytometry revealed the presence of CD63 and CD81 in all vesicles investigated, as well as CD9 except in the TF-1-derived vesicles, as these cells do not express CD9. Conclusions: Our results demonstrate that centrifugation-based protocols are simple and fast systems to distinguish subpopulations of extracellular vesicles. Different vesicles show different RNA profiles and morphological characteristics, but they are indistinguishable using CD63-coated beads for flow cytometry analysis

Orbital and physical parameters of eclipsing binaries from the ASAS catalogue - IX. Spotted pairs with red giants

We present spectroscopic and photometric solutions for three spotted systems with red giant components. Absolute physical and orbital parameters for these double-lined detached eclipsing binary stars are presented for the first time. These were derived from the V-, and I-band ASAS and WASP photometry, and new radial velocities calculated from high quality optical spectra we obtained with a wide range of spectrographs and using the two-dimensional cross-correlation technique (TODCOR). All of the investigated systems (ASAS J184949-1518.7, BQ Aqr, and V1207 Cen) show the differential evolutionary phase of their components consisting of a main-sequence star or a subgiant and a red giant, and thus constitute very informative objects in terms of testing stellar evolution models. Additionally, the systems show significant chromospheric activity of both components. They can be also classified as classical RS CVn-type stars. Besides the standard analysis of radial velocities and photometry, we applied spectral disentangling to obtain separate spectra for both components of each analysed system which allowed for a more detailed spectroscopic study. We also compared the properties of red giant stars in binaries that show spots, with those that do not, and found that the activity phenomenon is substantially suppressed for stars with Rossby number higher than ∼1 and radii larger than ∼20 R⊙

Collision Dynamics and Solvation of Water Molecules in a Liquid Methanol Film

Environmental molecular beam experiments are used to examine water interactions with liquid methanol films at temperatures from 170 K to 190 K. We find that water molecules with 0.32 eV incident kinetic energy are efficiently trapped by the liquid methanol. The scattering process is characterized by an efficient loss of energy to surface modes with a minor component of the incident beam that is inelastically scattered. Thermal desorption of water molecules has a well characterized Arrhenius form with an activation energy of 0.47{\pm}0.11 eV and pre-exponential factor of 4.6 {\times} 10^(15{\pm}3) s^(-1). We also observe a temperature dependent incorporation of incident water into the methanol layer. The implication for fundamental studies and environmental applications is that even an alcohol as simple as methanol can exhibit complex and temperature dependent surfactant behavior.Comment: 8 pages, 5 figure

Orbital and physical parameters of eclipsing binaries from the ASAS catalogue - IV. A 0.61 + 0.45 M_sun binary in a multiple system

We present the orbital and physical parameters of a newly discovered low-mass detached eclipsing binary from the All-Sky Automated Survey (ASAS) database: ASAS J011328-3821.1 A - a member of a visual binary system with the secondary component separated by about 1.4 seconds of arc. The radial velocities were calculated from the high-resolution spectra obtained with the 1.9-m Radcliffe/GIRAFFE, 3.9-m AAT/UCLES and 3.0-m Shane/HamSpec telescopes/spectrographs on the basis of the TODCOR technique and positions of H_alpha emission lines. For the analysis we used V and I band photometry obtained with the 1.0-m Elizabeth and robotic 0.41-m PROMPT telescopes, supplemented with the publicly available ASAS light curve of the system. We found that ASAS J011328-3821.1 A is composed of two late-type dwarfs having masses of M_1 = 0.612 +/- 0.030 M_sun, M_2 = 0.445 +/- 0.019 M_sun and radii of R_1 = 0.596 +/- 0.020 R_sun, R_2 = 0.445 +/- 0.024 R_sun, both show a substantial level of activity, which manifests in strong H_alpha and H_beta emission and the presence of cool spots. The influence of the third light on the eclipsing pair properties was also evaluated and the photometric properties of the component B were derived. Comparison with several popular stellar evolution models shows that the system is on its main sequence evolution stage and probably is more metal rich than the Sun. We also found several clues which suggest that the component B itself is a binary composed of two nearly identical ~0.5 M_sun stars.Comment: 12 pages, 7 figures, 7 tables, to appear in MNRA