An integrated view

Funding Information: This work was supported by “Fundação para a Ciência e a Tecnologia”—FCT MJM (PD/BD/114256/2016), MPM (PTDC/BIM-MET/4265/2014 and PTDC/MEC-MET/29314/2017), MGA (PTDC/BIM-MET/4712/2014), iNOVA4Health (UIDB/Multi/04462/2020), by the European Commission Marie Skłodowska-Curie Action H2020 (mtFOIE GRAS, grant agreement n. 734719), by the Sociedade Portuguesa de Diabetologia, and by the research infrastructure CONGENTO, project LISBOA-01-0145-FEDER-022170, co-financed by Lisboa Regional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund and by Fundação para a Ciência e Tecnologia (Portugal). NMR data were collected at the UC-NMR facility which is supported in part by FEDER – European Regional Development Fund through the COMPETE Programme (Operational Programme for Competitiveness) and by National Funds through FCT – Fundação para a Ciência e a Tecnologia (Portuguese Foundation for Science and Technology) through grants REEQ/481/QUI/2006, RECI/QEQ-QFI/0168/2012, CENTRO-07-CT62-FEDER-002012, and Rede Nacional de Ressonância Magnética Nuclear (RNRMN). Publisher Copyright: Copyright © 2022 Meneses, Sousa-Lima, Jarak, Raposo, Alves and Macedo.Objective: In the last years, changes in dietary habits have contributed to the increasing prevalence of metabolic disorders, such as non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM). The differential burden of lipids and fructose on distinct organs needs to be unveiled. Herein, we hypothesized that high-fat and high-fructose diets differentially affect the metabolome of insulin-sensitive organs such as the liver, muscle, and different adipose tissue depots. Methods: We have studied the impact of 12 weeks of a control (11.50% calories from fat, 26.93% from protein, and 61.57% from carbohydrates), high-fat/sucrose (HFat), or high-fructose (HFruct) feeding on C57Bl/6J male mice. Besides glucose homeostasis, we analyzed the hepatic levels of glucose and lipid-metabolism-related genes and the metabolome of the liver, the muscle, and white (WAT) and brown adipose tissue (BAT) depots. Results: HFat diet led to a more profound impact on hepatic glucose and lipid metabolism than HFruct, with mice presenting glucose intolerance, increased saturated fatty acids, and no glycogen pool, yet both HFat and HFruct presented hepatic insulin resistance. HFat diet promoted a decrease in glucose and lactate pools in the muscle and an increase in glutamate levels. While HFat had alterations in BAT metabolites that indicate increased thermogenesis, HFruct led to an increase in betaine, a protective metabolite against fructose-induced inflammation. Conclusions: Our data illustrate that HFat and HFruct have a negative but distinct impact on the metabolome of the liver, muscle, WAT, and BAT.publishersversionpublishe

Lifestyle, metabolic disorders and male hypogonadism - a one-way ticket?

Hypogonadism is more frequent among men with common metabolic diseases, notably obesity and type 2 diabetes. Indeed, endocrine disruption caused by metabolic diseases can trigger the onset of hypogonadism, although the underlying molecular mechanisms are not entirely understood. Metabolic diseases are closely related to unhealthy lifestyle choices, such as dietary habits and sedentarism. Therefore, hypogonadism is part of a pathological triad gathering unhealthy lifestyle, metabolic disease and genetic background. Additionally, hypogonadism harbors the potential to aggravate underlying metabolic disorders, further sustaining the mechanisms leading to disease. To what extent does lifestyle intervention in men suffering from these metabolic disorders can prevent, improve or reverse hypogonadism, is still controversial. Moreover, recent evidence suggests that the metabolic status of the father is related to the risk of inter and transgenerational inheritance of hypogonadism. In this review, we will address the proposed mechanisms of disease, as well as currently available interventions for hypogonadism.publishe

Atas das 8as Jornadas de Segurança aos Incêndios Urbanos e as 3as Jornadas de Proteção Civil (8JORNINC-3JORPROCIV)

Este livro de ATAS contém os artigos apresentados às 8as Jornadas de Segurança aos Incêndios Urbanos e às 3as Jornadas de Proteção Civil (8JORNINC-3JORPROCIV), que decorreram no Porto, Portugal. Na presente edição das 8JORNINC-3JORPROCIV foram submetidos 50 trabalhos, tendo sido aceites 42. Os trabalhos foram distribuídos em 8 sessões paralelas temáticas, em adição a 2 sessões plenárias, apresentados no dia 2 de junho de 2023. O evento foi iniciado e promovido em Portugal, sob a organização do professor Doutor João Paulo Rodrigues, com as 1as Jornadas de Segurança aos Incêndios Urbanos, em 2005, na Universidade de Coimbra, bem como nos anos seguintes, as 2as Jornadas de Segurança aos Incêndios Urbanos em 2011 e as 3as Jornadas de Segurança aos Incêndios Urbanos em 2013, também na Universidade de Coimbra. As edições seguintes aconteceram em diferentes locais: as 4as Jornadas de Segurança aos Incêndios Urbanos em 2014, no Instituto Politécnico de Bragança; as 5as Jornadas de Segurança aos Incêndios Urbanos em 2016, no Laboratório Nacional de Engenharia Civil em Lisboa; as 6as Jornadas de Segurança aos Incêndios Urbanos e 1as Jornadas de Proteção Civil em 2018, na Universidade de Coimbra; e as 7as Jornadas de Segurança aos Incêndios Urbanos e 2as Jornadas de Proteção Civil, em 2021, no Instituto Politécnico de Castelo Branco. As 8JORNINC apresentam grande importância num contexto atual de vários e graves incêndios urbanos, florestais e de interface em Portugal. A pertinência do tema da segurança na prevenção e no combate a incêndios, quer pelas consequências emergentes deste tipo de acidentes, quer pela necessidade de redução das ocorrências, do número de vítimas mortais, feridos, prejuízos materiais, patrimoniais, ambientais e sociais, leva a que as Jornadas se destinem a um leque alargado de profissionais e público em geral. As 3JORPROCIV têm como objetivo promover conhecimentos nas áreas da prevenção civil, de riscos e planos de emergência. Pretendem assim, contribuir para a atualização dos conhecimentos técnicos e científicos da segurança e proteção civil, no âmbito do planeamento e prevenção perante cenários de crise e emergência. As Jornadas de Proteção Civil permitem partilhar um leque de conhecimentos multidisciplinares suscetíveis de impulsionar uma intervenção por parte de técnicos, especialistas e dos agentes da proteção civil. A visão interdisciplinar e integradora dos problemas e desafios que a proteção civil apresenta será refletida na prevenção e mitigação dos riscos inerentes a uma situação de acidente. Os trabalhos apresentados nesta edição, permitirão o avanço das 8JORNINC-3JORPROCIV, através da divulgação dos recentes desenvolvimentos e do conhecimento nos domínios da segurança ao incêndio e proteção civil. Por fim, a comissão organizadora das 8JORNINC-3JORPROCIV gostaria de agradecer: - o apoio dos patrocinadores e das instituições do Sistema Científico e Tecnológico; - a todos os autores que partilharam os seus excelentes trabalhos; - e aos elementos da Comissão Científica que auxiliaram no processo de revisão. Todos em conjunto, tornaram possível a realização destas Jornadas.info:eu-repo/semantics/publishedVersio

5´-alkoxy-2,2´-bithiophene azo dyes: a novel promising series of NLO chromophores

A series of bithienyl azo dyes has been prepared from their corresponding coupling components, 5-alkoxy-2,2´-bithiophenes. The solvatochromic behavior of the compounds was investigated. The hyperpolarizabilities β of derivatives 3-5 were measured using hyper-Rayleigh scattering and thermogravimetric analysis (TGA) was used to evaluate their thermal stability. The experimental results indicate that, good nonlinearity-thermal stability is well balanced for azo-bithiophene NLO-chromophores 3-5 making them good candidates for NLO applications.Fundação para a Ciência e Tecnologia

Inheritable testicular metabolic memory of high-fat diet causes transgenerational sperm defects in mice

Funding Information: This work was supported by the Portuguese Foundation for Science and Technology: L. Crisóstomo (SFRH/ BD/128584/2017), M.G. Alves (IFCT2015 and PTDC/MEC-AND/28691/2017), P.F. Oliveira (IFCT2015), UMIB (UIDB/00215/2020 and UIDP/00215/2020) and QOPNA (UID/QUI/00062/2019) co-funded by FEDER funds (POCI/COMPETE 2020); by the Portuguese Society of Diabetology: L. Crisóstomo and M.G. Alves (“Nuno Castel-Branco” research grant and Group of Fundamental and Translational Research); and by the European Foundation for the Study of Diabetes: L. Crisóstomo (Albert Renold Travel Grant). NMR data was collected at the UC-NMR facility which is supported in part by FEDER—European Regional Development Fund through the COMPETE Programme (Operational Programme for Competitiveness) and by National Funds through FCT— Fundação para a Ciência e a Tecnologia (Portuguese Foundation for Science and Technology) through grants REEQ/481/QUI/2006, RECI/QEQ-QFI/0168/2012, CENTRO-07-CT62-FEDER-002012, and Rede Nacional de Ressonância Magnética Nuclear (RNRMN). We thank Prof. Pedro N. Oliveira (University of Porto, Portugal) and Matthieu Bourgery (University of Turku, Finland) for their advice in Statistical Analysis. Publisher Copyright: © 2021, The Author(s). Copyright: Copyright 2021 Elsevier B.V., All rights reserved.The consumption of energy-dense diets has contributed to an increase in the prevalence of obesity and its comorbidities worldwide. The adoption of unhealthy feeding habits often occurs at early age, prompting the early onset of metabolic disease with unknown consequences for reproductive function later in life. Recently, evidence has emerged regarding the intergenerational and transgenerational effects of high-fat diets (HFD) on sperm parameters and testicular metabolism. Hereby, we study the impact of high-fat feeding male mice (F0) on the testicular metabolome and function of their sons (F1) and grandsons (F2). Testicular content of metabolites related to insulin resistance, cell membrane remodeling, nutritional support and antioxidative stress (leucine, acetate, glycine, glutamine, inosine) were altered in sons and grandsons of mice fed with HFD, comparing to descendants of chow-fed mice. Sperm counts were lower in the grandsons of mice fed with HFD, even if transient. Sperm quality was correlated to testicular metabolite content in all generations. Principal Component Analysis of sperm parameters and testicular metabolites revealed an HFD-related phenotype, especially in the diet-challenged generation and their grandsons. Ancestral HFD, even if transient, causes transgenerational “inherited metabolic memory” in the testicular tissue, characterized by changes in testicular metabolome and function.publishersversionpublishe

Impact of combined training with different exercise intensities on inflammatory and lipid markers in type 2 diabetes : a secondary analysis from a 1-year randomized controlled trial

© The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creat ivecommons .org/licen ses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creat iveco mmons .org/publi cdoma in/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.Background: Exercise is a well-accepted strategy to improve lipid and infammatory profle in individuals with type 2 diabetes (T2DM). However, the exercise intensity having the most benefts on lipids and infammatory markers in patients with T2DM remains unclear. We aimed to analyse the impact of a 1-year combined high-intensity interval training (HIIT) with resistance training (RT), and a moderate continuous training (MCT) with RT on infammatory and lipid profle in individuals with T2DM. Methods: Individuals with T2DM (n=80, aged 59 years) performed a 1-year randomized controlled trial and were randomized into three groups (control, n=27; HIIT with RT, n=25; MCT with RT, n=28). Exercise sessions were super‑ vised with a frequency of 3 days per week. Infammatory and lipid profles were measured at baseline and at 1-year follow-up. Changes in infammatory and lipid markers were assessed using generalized estimating equations. Results: After adjusting for sex, age and baseline moderate-to-vigorous physical activity (MVPA), we observed a time-by-group interaction for Interleukin-6 (IL-6) in both the MCT with RT (β=−0.70, p=0.034) and HIIT with RT (β=−0.62, p=0.049) groups, whereas, only the HIIT with RT group improved total cholesterol (β=−0.03, p=0.045) and LDL-C (β=−0.03, p=0.034), when compared to control. No efect was observed for C-reactive protein (CRP), cortisol, tumour necrosis factor-α (TNF-α), soluble form of the haptoglobin-hemoglobin receptor CD163 (sCD163), triglycerides and HDL-C in both groups (p>0.05). Conclusions: Favorable adaptations on IL-6 were observed in both the HIIT and MCT combined with RT groups fol‑ lowing a long-term 1-year exercise intervention in individuals with T2DM. However, only the HIIT with RT prevented further derangement of total cholesterol and LDL-C, when compared to the control group. Therefore, in order to encourage exercise participation and improve infammatory profle, either exercise protocols may be prescribed, however, HIIT with RT may have further benefts on the lipid profle.This work was supported by fellowships from the Portuguese Foundation for Science and Technology (JM grant: SFRH/BD/85742/2012; IRC grant: IRC: SFRH/BD/149394/2019). This work is also fnanced by a national grant through the FCT–Fundação para a Ciência e Tecnologia within the unit I&D 447 (UIDB/00447/2020).info:eu-repo/semantics/publishedVersio

A synthesis of 8,10-dimethoxyellipticine via a diphenylamine

8,10-Dimethoxyellipticine has been synthesised from readily available benzene derivatives via palladium acetate or photochemical cyclisation of intermediate diphenylamine derivatives. The route has advantages over indole based syntheses.We thank the British Council for awards under the Treaty of Windsor Programme and JNICT (IBQF - UM) Portugal for financial support.info:eu-repo/semantics/publishedVersio