Cyst nematodes of the genus Heterodera and Globodera in Sicily

A survey was conducted in Sicily, from 2007 to 2009, to obtain insights on specific composition, frequency and geographical distribution of cyst nematodes associated to crops of agricultural economic importance. Soil and root samples were collected mainly from crops showing chlorosis, poor growth and reduced yield. Cysts and larvae were extracted and identified to species level by comparative examination of morphological and morphometric characters. Confirmation of the species identity was also made by molecular analysis for some species. Seven species of the genus Heterodera Schmidt, 1871 and two of the genus Globodera Skarbilovic, 1959 were found. They were H. avenae Wollenweber, 1924, H. bifenestra Cooper, 1955, H. carotae Jones, 1950, H. goettingiana Liebscher, 1892, H. hordecalis Andersson, 1974, H. latipons Franklin, 1969 and H. schachtii Schmidt, G. rostochiensis (Wollenweber, 1923) Behrens, 1975 and G. pallida (Stone, 1973) Behrens, 1975. All species dectected are known to occur in Europe and the Mediterranean basin, but H. avenae, H. hordecalis, and H. latipons had not been reported from Sicily and H. bifenestra is a new report for Italy

Severe damage caused by Meloidogyne artiellia on cereals and leguminous in Sicily

The root-knot nematode Meloidogyne artiellia Franklin has been reported in various Italian regions on host plants belonging to the families Graminaceae, Leguminosae and Cruciferae, on which it is responsible for severe yield losses. It had never been detected in Sicily. In spring 2008, the nematode was found in different legume and cereal fields in the area of Caltagirone (province of Catania, Sicily). Fields infested with the nematode showed patches of stunted, chlorotic, withered and dwarfed plants. Moreover, ears of wheat were small and kernels appeared dried; finally, roots were scrubby. Infected plants were durum wheat (Triticum durum Desf.), barley (Hordeum vulgare L.), chickpea (Cicer arietinum L.), broad bean (Vicia faba L.), lentil (Lens culinaris Medik.) and vetch (Vicia sativa L.) Root observations showed brownings, small galls and mature females bearing egg masses and larvae at root bifurcations

A Quantitative Morphological Analysis of Some Hypericum Species

Hypericum perforatum L. (Hypericaceae) is a medicinal plant of considerable interest for the therapeutic potentialities of its biologically active compounds. Due to the presence of hybrids and frequent adulterants from other species of Hypericum, the identification of the drug obtained of this species is difficult. Therefore, a quantitative morphological analysis of the leaf epidermises of H. hircinum L. and H. perfoliatum L. compared with H. perforatum L., carried out by means of scanning electron microscopy and image analysis, was performed to identify phytognostic markers useful for the characterization of these different Hypericum species. Size and shape parameters of the leaf surface cells have permitted a comparative study of the cogeneric species examined, providing a key factor in their recognition and/or selection. Unlike the methods employed so far, the results obtained by means of this innovative kind of analysis supply a valid criterion, not only for the morphological differentiation of the Italian Hypericum species studied, but also for an accurate and reproducible quality control of the commercial samples, often made up of drugs obtained from different species, subspecies and varieties

Clinical Aspects of Manic Episodes After SARS-CoV-2 Contagion or COVID-19

As COVID-19 pandemic spread all over the world, it brought serious health consequences in every medical field, including mental health. Not only healthcare professionals were more prone to develop anxiety, depression, and stress, but the general population suffered as well. Some of those who had no prior history of a psychiatric disease developed peculiar symptoms following infection with SARS-CoV-2, mostly because of psychological and social issues triggered by the pandemic. People developed traumatic memories, and hypochondria, probably triggered by social isolation and stress. Infection with SARS-CoV-2 has influenced the mental health of psychiatric patients as well, exacerbating prior psychiatric conditions. In this review, we focus on analyzing those cases of mania in the context of bipolar disorder (BD) reported after COVID-19 disease, both in people with no prior psychiatric history and in psychiatric patients who suffered an exacerbation of the disease. Results have shown that COVID-19 may trigger a pre-existing BD or unmask an unknown BD, due to social and psychological influences (decreased social interaction, change in sleep patterns) and through biological pathways both (neuroinflammation and neuroinvasion through ACE-2 receptors expressed in the peripheral and central nervous systems (PNS and CNS respectively). No direct correlation was found between the severity of COVID-19 disease and manic symptoms. All cases presenting severe symptoms of both diseases needed specific medical treatment, meaning that they concur but are separate in the treatment strategy needed. This review highlights the importance of a now widespread viral disease as a potential agent unmasking and exacerbating bipolar mood disorder, and it can hopefully help physicians in establishing a rapid diagnosis and treatment, and pave the road for future research on neuroinflammation triggered by SARS-CoV-2

Short-term use of dexamethasone/netilmicin fixed combination in controlling ocular inflammation after uncomplicated cataract surgery

Purpose: To evaluate the short-term anti-inflammatory effect of dexamethasone/netilmicin fixed combination in the management of ocular inflammation after cataract surgery. Patients and methods: Open-label, randomized, active-controlled, clinical study conducted in 6 sites in Italy; 238 patients were randomized 2:1 to dexamethasone/netilmicin (dexa/net, n=158) or betamethasone/chloramphenicol (beta/chl, n=80). Treatment started the day of surgery and continued 4 times daily for 7 days. The primary efficacy parameter was the anterior chamber (AC) flare. The percentage of patients displaying none or mild (ie, only barely detectable) AC flare was defined as "efficacy rate", whereas the percentage of patients showing a decrease of AC flare score from baseline was defined as "percentage of responders". Additional parameters evaluated were AC cells, conjunctival hyperaemia, corneal and lid oedema, symptoms of ocular discomfort, visual acuity, and intraocular pressure. Dexa/net was considered effective if the efficacy rate was not inferior (by means of 97.5% confidence interval) to that of beta/chl. Results: After 7 days of treatment, no AC flare was observed in 92.8% (dexa/net) and 92.3% (beta/chl) of patients, whereas no AC cells were observed in 91.5% (dexa/net) and 93.6% (beta/chl) of patients, respectively. The "efficacy rate" was 100% in both groups, whereas the "percentage of responders" was 94.1% in the dexa/net and 93.6% in the beta/chl group. The p-value to reject the null hypothesis of inferiority was <0.001. Other efficacy parameters confirmed both treatments as highly effective, despite their difference in steroid content (2 mg/mL for beta/chl vs 1 mg/mL for dexa/net). IOP and visual acuity at the end of the study were comparable. Two cases of allergic conjunctivitis were considered adverse events and were both related to dexa/net. Conclusion: Short-term use of dexa/net fixed combination is safe and effective in the control of post-operative inflammation following uncomplicated cataract surgery

Work-related stress in the italian banking population and it's association with recovery experience

Objectives: Over the past decade, there has been a markedly growing interest in issues involving work-related stress in Europe. In Italy, according to Eurofound, research has demonstrated high levels of stress in the banking sector. With this in mind, a study was conducted to evaluate the peculiarities of hindrance and challenge stressors, and their links with recovery in the Italian banking population. Material and Methods: The health and safety managers of a major Italian banking group were contacted and invited to participate in a stress assessment procedure, not only for research purposes but also to help them fulfill their obligations with regard to work-related stress. In total, 6588 bank workers took part in the study (57.5% males and 42.5% females) in 2012–2018. Work-related stress was measured with the Stress Questionnaire (SQ) that assesses several psychosocial working variables. Recovery was measured using a scale based on the SQ. The analysis and tabulation of the study results were performed using the Statistical Package for Social Sciences (SPSS). Results: The results have demonstrated that female respondents lack more job control and colleagues’ support as compared to male respondents. Employees aged > 50 lack their supervisors’ support while employees with the shortest job seniority experience the greatest role ambiguity, as well as the lack of job control and colleagues’ support. The results of hierarchical regression analysis have demonstrated that the lack of colleagues and supervisors’ support, as well as job demands and job control, contribute to explaining the recovery experience. The greatest contribution to the explained variance could be attributed to job demands and the lack of colleagues’ support. Conclusions: This study has demonstrated an important contribution of the so-called traditional stressors to predicting recovery for the banking population in Italy. These findings suggest that banks should adopt corporate policies containing activities for the prevention of and protection against stress, with a more general objective of improving the mental health of their workers

Association between polyphenol intake and adherence to the Mediterranean diet in Sicily, southern Italy

Abstract Background Mediterranean diet has been demonstrated to exert beneficial effects toward various health outcomes. Among the compounds that may be responsible for such benefits, polyphenols have been proposed as potential candidates. The aim of this study was to evaluate whether dietary polyphenols were associated with adherence to the Mediterranean diet in a Sicilian cohort. Methods A total of 1937 adults were recruited in the urban area of Catania, southern Italy. Background characteristics and dietary habits were collected through validated questionnaires. Adherence to the Mediterranean diet was evaluated through application of a validated score (MEDI-LITE score). Dietary intake of polyphenols was estimated through the Phenol-explorer database. Differences in mean intake between quartiles of the MEDI-LITE score and association between quartiles of polyphenol intake and high adherence to the Mediterranean diet (highest quartile of the score) were calculated though logistic regression analyses. Results Mean intake of most polyphenols was significantly different between quartiles of the MEDI-LITE score, being generally higher in individuals more adherent to the Mediterranean diet. Only few compounds, such as lignans, anthocyanins, and flavanones, showed a linear positive association with high adherence to the Mediterranean diet, while other polyphenol classes were associated in a non-linear manner. Among individual polyphenols, apigenin, hesperetin, naringenin, lariciresinol, matairesinol, pinoresinol, secoisolariciresinol, and ferulic acid were associated with high adherence to Mediterranean diet in a linear manner, while all the others (except for myricetin) were associated in a non-linear way. Conclusions Mean polyphenol intake was higher in individuals more adherent to the Mediterranean diet compared to less adherent. However, dietary sources of polyphenols not included in the traditional foods comprised in the Mediterranean diet may contribute to total and specific classes of polyphenols irrespectively of their inclusion within the context of the Mediterranean diet

Structural compatibility between the putative voltage sensor of voltage-gated K+ channels and the prokaryotic KcsA channel.

Sequence similarity among and electrophysiological studies of known potassium channels, along with the three-dimensional structure of the Streptomyces lividans K(+) channel (KcsA), support the tenet that voltage-gated K(+) channels (Kv channels) consist of two distinct modules: the "voltage sensor" module comprising the N-terminal portion of the channel up to and including the S4 transmembrane segment and the "pore" module encompassing the C-terminal portion from the S5 transmembrane segment onward. To substantiate this modular design, we investigated whether the pore module of Kv channels may be replaced with the pore module of the prokaryotic KcsA channel. Biochemical and immunocytochemical studies showed that chimeric channels were expressed on the cell surface of Xenopus oocytes, demonstrating that they were properly synthesized, glycosylated, folded, assembled, and delivered to the plasma membrane. Unexpectedly, surface-expressed homomeric chimeras did not exhibit detectable voltage-dependent channel activity upon both hyperpolarization and depolarization regardless of the expression system used. Chimeras were, however, strongly dominant-negative when coexpressed with wild-type Kv channels, as evidenced by the complete suppression of wild-type channel activity. Notably, the dominant-negative phenotype correlated well with the formation of stable, glycosylated, nonfunctional, heteromeric channels. Collectively, these findings imply a structural compatibility between the prokaryotic pore module and the eukaryotic voltage sensor domain that leads to the biogenesis of non-responsive channels. Our results lend support to the notion that voltage-dependent channel gating depends on the precise coupling between both protein domains, probably through a localized interaction surface

Rapid identification of Trialeurodes vaporariorum, Bemisia tabaci (MEAM1 and MED) and tomato-infecting criniviruses in whiteflies and in tomato leaves by real-time reverse transcription-PCR assay

Abstract The whiteflies Bemisia tabaci and Trialeurodes vaporariorum (Hemiptera Aleyrodidae) are harmful pests of vegetable and ornamental crops in many countries. Also, they are vectors of emergent viruses on tomato including the criniviruses (Closteroviridae genus Crinivirus) Tomato chlorosis virus (ToCV) and Tomato infectious chlorosis virus (TICV). Since different vectors are involved in the transmission of both viruses (ToCV is transmitted by B. tabaci, Trialeurodes abutiloneus and T. vaporariorum while TICV is transmitted only by T. vaporariorum), and they induce similar symptoms on tomato plants, a sensitive and specific diagnosis method is desirable. In addition, a rapid discriminating method of the vectors is essential for monitoring and control activities and epidemiological studies. For these reasons, a combined protocol based on one-step multiplex real-time reverse transcription (RT)-PCR has been developed for the identification of T. vaporariorum, two invasive species of the complex B. tabaci (MEAM1 and MED) and for the specific detection of ToCV and TICV in whiteflies and plants