1,122 research outputs found
Assessment of eosinophils in gastrointestinal inflammatory disease of dogs
Background
Accurate identification of eosinophils in the gastrointestinal (GI) tract of dogs with eosinophilic GI disease (EGID) by histological evaluation is challenging. The currently used hematoxylin and eosin (H&E) staining method detects intact eosinophils but does not detect degranulated eosinophils, thus potentially underrepresenting the number of infiltrating eosinophils.
Objective
To develop a more sensitive method for identifying and quantifying both intact and degranulated eosinophils to diagnose EGID more accurately.
Methods
Endoscopically obtained paraffinâembedded intestinal biopsy specimens from dogs with GI signs were examined. The study groups were dogs with eosinophilic enteritis (EE), lymphoplasmacytic and mixed enteritis, and control dogs with GI signs but no histologic changes on tissue sections. Consecutive sections were immunolabeled with monoclonal antibodies (mAbs) against the eosinophil granule protein eosinophil peroxidase (Epx) and stained by H&E, respectively. The number of eosinophils was manually quantified and classified as intact or degranulated.
Results
The number of intact eosinophils detected in Epx mAbâlabeled duodenal sections was significantly higher compared with that in H&Eâstained sections, with a similar relationship noted in the colon and stomach. The Epx mAb allowed the unique assessment of eosinophil degranulation. The number of intact and degranulated eosinophils was significantly higher in duodenal lamina propria of the EE and mixed group compared to the control group.
Conclusion
Immunohistochemical detection of Epx provides a more precise method to detect GI tract eosinophils compared to H&E staining and could be used as an alternative and reliable diagnostic tool for assessment of biopsy tissues from dogs with EGID
Interfering With Contextual Fear Memories by Post-reactivation Administration of Propranolol in Mice: A Series of Null Findings
An Integrated Declarative Approach to Web Services Composition and Monitoring
International audienceIn this paper we propose a constraint based declarative approach for Web services composition and monitoring problem. Our approach allows user to build the abstract composition by identifying the participating entities and by providing a set of constraints that mark the boundary of the solution. Different types of constraints have been proposed to handle the composition modeling and monitoring. Abstract composition is then used for instantiating the concrete composition, which both finds and executes an instantiation respecting constraints, and also handles the process run-time monitoring. When compared to the traditional approaches, our approach is declarative and allows for the same set of constraints to be used for composition modeling and monitoring and thus allows for refining the abstract composition as a result of run-time violations, such as service failure or response time delays
Nonintegrable Interaction of Ion-Acoustic and Electromagnetic Waves in a Plasma
In this paper we re-examine the one-dimensional interaction of
electromagnetic and ion acoustic waves in a plasma. Our model is similar to one
solved by Rao et al. (Phys. Fluids, vol. 26, 2488 (1983)) under a number of
analytical approximations. Here we perform a numerical investigation to examine
the stability of the model. We find that for slightly over dense plasmas, the
propagation of stable solitary modes can occur in an adiabatic regime where the
ion acoustic electric field potential is enslaved to the electromagnetic field
of a laser. But if the laser intensity or plasma density increases or the laser
frequency decreases, the adiabatic regime loses stability via a transition to
chaos. New asymptotic states are attained when the adiabatic regime no longer
exists. In these new states, the plasma becomes rarefied, and the laser field
tends to behave like a vacuum field.Comment: 19 pages, REVTeX, 6 ps figures, accepted for publication in Phys.
Rev.
A Honeycomb Proportional Counter for Photon Multiplicity Measurement in the ALICE Experiment
A honeycomb detector consisting of a matrix of 96 closely packed hexagonal
cells, each working as a proportional counter with a wire readout, was
fabricated and tested at the CERN PS. The cell depth and the radial dimensions
of the cell were small, in the range of 5-10 mm. The appropriate cell design
was arrived at using GARFIELD simulations. Two geometries are described
illustrating the effect of field shaping. The charged particle detection
efficiency and the preshower characteristics have been studied using pion and
electron beams. Average charged particle detection efficiency was found to be
98%, which is almost uniform within the cell volume and also within the array.
The preshower data show that the transverse size of the shower is in close
agreement with the results of simulations for a range of energies and converter
thicknesses.Comment: To be published in NIM
Photoconductance Quantization in a Single-Photon Detector
We have made a single-photon detector that relies on photoconductive gain in
a narrow electron channel in an AlGaAs/GaAs 2-dimensional electron gas. Given
that the electron channel is 1-dimensional, the photo-induced conductance has
plateaus at multiples of the quantum conductance 2e/h. Super-imposed on
these broad conductance plateaus are many sharp, small, conductance steps
associated with single-photon absorption events that produce individual
photo-carriers. This type of photoconductive detector could measure a single
photon, while safely storing and protecting the spin degree of freedom of its
photo-carrier. This function is valuable for a quantum repeater that would
allow very long distance teleportation of quantum information.Comment: 4 pages, 4 figure
Stationary State Solutions of a Bond Diluted Kinetic Ising Model: An Effective-Field Theory Analysis
We have examined the stationary state solutions of a bond diluted kinetic
Ising model under a time dependent oscillating magnetic field within the
effective-field theory (EFT) for a honeycomb lattice . Time evolution of
the system has been modeled with a formalism of master equation. The effects of
the bond dilution, as well as the frequency and amplitude of
the external field on the dynamic phase diagrams have been discussed in detail.
We have found that the system exhibits the first order phase transition with a
dynamic tricritical point (DTCP) at low temperature and high amplitude regions,
in contrast to the previously published results for the pure case \cite{Ling}.
Bond dilution process on the kinetic Ising model gives rise to a number of
interesting and unusual phenomena such as reentrant phenomena and has a
tendency to destruct the first-order transitions and the DTCP. Moreover, we
have investigated the variation of the bond percolation threshold as functions
of the amplitude and frequency of the oscillating field.Comment: 8 pages, 4 figure
Non-linear electrical response in a charge/orbital ordered CaMnO crystal : the charge density wave analogy
Non-linear conduction in a charge-ordered manganese oxide
PrCaMnO is reported. To interpret such a feature, it is
usually proposed that a breakdown of the charge or orbitally ordered state is
induced by the current. The system behaves in such a way that the bias current
may generate metallic paths giving rise to resistivity drop. One can describe
this feature by considering the coexistence of localized and delocalized
electron states with independent paths of conduction. This situation is
reminiscent of what occurs in charge density wave systems where a similar
non-linear conduction is also observed. In the light of recent experimental
results suggesting the development of charge density waves in charge and
orbitally ordered manganese oxides, a phenomenological model for charge density
waves motion is used to describe the non-linear conduction in
PrCaMnO. In such a framework, the non-linear conduction
arises from the motion of the charge density waves condensate which carries a
net electrical current.Comment: 13 pages, 6 figure
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