Plant and soil metal concentrations in serpentine soils and their influence on the diet of extensive livestock animals

Background: Grazing circuits and resources consumed differ strongly throughout the year and within a territory. For this reason, animals’ diet composition, as well as their exposure to metals, is variable. No studies have been performed on how habitat use affects the metal concentrations to which sheep and goats reared in serpentine soil areas are exposed. Objective: The aim of the present study was to investigate the metal exposure of grazing animals raised in a serpentine soil area of the north-east of Portugal, taking into account the spatial distribution of metal concentrations in soils and plants. Methods: The habitat use and foraging behaviour of six flocks of sheep and goats were studied. The concentrations of Ca, Mg, Mn, Cr and Ni were determined in the soils and plant species most consumed by those animals. Results: The highest Mg, Mn, Cr and Ni concentrations were found in the soils of the ultramafic complex. Ni concentrations above the recommended threshold for agricultural soils (30 μg/g) were found in some sites. A positive correlation between Ni concentration in soils and plants was found (0.634). Ni concentrations higher than 10 μg/g were found in some samples of the following plant species: Sorghum × drummondii (Steud.) Millsp. & Chase,Quercus rotundifolia Lam., Cytisus multiflorus (L’Hér.) Sweet, Cistus ladanifer L. and Erica scoparia L. Significant differences (p ≤ 0.05) in metal concentrations of the plants most consumed by each flock were observed. Conclusion: Grazing circuits have an important role in the metal exposure of animals raised in this serpentine soil area.info:eu-repo/semantics/publishedVersio

Life-cycle greenhouse gas emissions of portuguese olive oil

The main goal of this paper was to assess the greenhouse gas (GHG) intensity of olive oil production in Portugal. A life-cycle model and inventory were implemented for the entire production process, including a comprehensive analysis of olive cultivation, olive oil extraction, packaging, and distribution. Data originates from five differently-sized Portuguese olive growers and from a total of six olive oil mills, representing the three extraction processes in use: three-phase extraction, two-phase extraction, and traditional pressing. The results show that the GHG intensity lies in the range 1.8-8.2 kg CO2eq/liter and that the main contributors were fertilizers (production and field emissions). Efficient use of fertilizers thus seems to be a key factor for mitigating the GHG intensity of olive oil production.This research was supported by project ECODEEP (Eco-efficiency and Eco-management in the Agro Industrial sector, FCOMP–05–0128–FEDER–018643) and the Portuguese Scienc

Diversity and patterns of marine non‐native species in the archipelagos of Macaronesia

Aims The present study is the first attempt to grasp the scale and richness of marine biological invasions in Macaronesia. We pioneered a comprehensive non-native species (NNS), inventory in the region to determine their diversity patterns and native distribution origins. NNS were defined here as the result of both introductions and range expansions. We also used statistical modelling to examine relationships among NNS richness, anthropogenic activities, demographic and geographical variables across Macaronesia. Location Macaronesia. Methods A comprehensive literature search was conducted for marine NNS records in Macaronesia, registering the first record's location and year from 1884 to 2020. We used univariate and multivariate analyses to evaluate differences and similarities in community composition. By applying a Generalized Linear Model (GLM), we tested hypotheses regarding NNS richness as a function of anthropogenic activities, demographic and geographical variables. Results A total of 144 marine non-native species (NNS) were recorded for the whole of Macaronesia. The highest NNS richness was registered in the Canary Islands (76 NNS), followed by the Azores (66 NNS), Madeira (59 NNS) and finally Cabo Verde (18 NNS). Some differences amongst archipelagos were observed, such as the high number of non-native macroalgae in the Azores, fishes in the Canary Islands and tunicates in Cabo Verde. Overall, macroalgae, tunicates and bryozoans were the predominant taxonomic groups in the Macaronesian archipelagos. Madeira and Canary Islands were the archipelagos with more similarity in marine NNS, and Cabo Verde the most divergent. Finally, GLM suggested that non-native richness patterns across Macaronesia were dependent on the considered archipelago and strongly affected by (1) minimum distance to the mainland, (2) the total number of ports and marinas and (3) total marinas area (km2). Conclusions The model results and NNS listing in the present study will likely raise the awareness and response regarding marine NNS in the whole Macaronesia region, serving as a baseline for future research as well as implementing and enforcing regulations related to the introduction of marine NNS in oceanic islands

Gastrointestinal dysfunction in patients and mice expressing the autism-associated R451C mutation in neuroligin-3

Gastrointestinal (GI) problems constitute an important comorbidity in many patients with autism. Multiple mutations in the neuroligin family of synaptic adhesion molecules are implicated in autism, however whether they are expressed and impact GI function via changes in the enteric nervous system is unknown. We report the GI symptoms of two brothers with autism and an R451C mutation in Nlgn3 encoding the synaptic adhesion protein, neuroligin-3. We confirm the presence of an array of synaptic genes in the murine GI tract and investigate the impact of impaired synaptic protein expression in mice carrying the human neuroligin-3 R451C missense mutation (NL3R451C ). Assessing in vivo gut dysfunction, we report faster small intestinal transit in NL3R451C compared to wild-type mice. Using an ex vivo colonic motility assay, we show increased sensitivity to GABAA receptor modulation in NL3R451C mice, a well-established Central Nervous System (CNS) feature associated with this mutation. We further show increased numbers of small intestine myenteric neurons in NL3R451C mice. Although we observed altered sensitivity to GABAA receptor modulators in the colon, there was no change in colonic neuronal numbers including the number of GABA-immunoreactive myenteric neurons. We further identified altered fecal microbial communities in NL3R451C mice. These results suggest that the R451C mutation affects small intestinal and colonic function and alter neuronal numbers in the small intestine as well as impact fecal microbes. Our findings identify a novel GI phenotype associated with the R451C mutation and highlight NL3R451C mice as a useful preclinical model of GI dysfunction in autism. LAY SUMMARY: People with autism commonly experience gastrointestinal problems, however the cause is unknown. We report gut symptoms in patients with the autism-associated R451C mutation encoding the neuroligin-3 protein. We show that many of the genes implicated in autism are expressed in mouse gut. The neuroligin-3 R451C mutation alters the enteric nervous system, causes gastrointestinal dysfunction, and disrupts gut microbe populations in mice. Gut dysfunction in autism could be due to mutations that affect neuronal communication.This work was supported by an Idea Development Award from the United States Department of Defense’s Congressionally Directed Medical Research Programs (CDMRP) Autism Research Program (AR110134) to E.L.H.-Y. and J.C.B.; the Victorian Government through the Operational Infrastructure Scheme, National Health and Medical Research Council (NHMRC) project grants (APP566642 to J.C.B. and APP1047674 to E.L.H.-Y.) and the Royal Melbourne Hospital Neuroscience Foundation. E.L.H.-Y. also received an ARC Future Fellowship (FT160100126) and an RMIT Vice Chancellor’s Senior Research Fellowship which supported G.K.B. and S.H. T.S., P.U., and N.Y. were funded by grants RO1AI100914, P30-DK56338, and U01-AI24290 awards to Baylor College of Medicine funded from the National Institute of Allergy and Infectious Diseases and National Institute of Diabetes and Digestive and Kidney Diseases at the National Institutes of Health (T.C.S.)

Avaliação do "shrinkage" ao longo da secagem por convecção de castanha (Castanea sativa)

info:eu-repo/semantics/publishedVersio

Use of response surface methodology (Rsm) for the identification of the best extraction conditions for headspace solid-phase micro extraction (hs-spme) of the volatile profile of cv. arbequina extra-virgin olive oil

The effect of the experimental conditions on assessing the global volatile profile of extra virgin olive oils (EVOO) by headspace solid-phase micro-extraction/gas chromatography-mass spectrometry (HS-SPME/GC–MS) is studied to obtain maximization of the total peak areas of the compounds. Response Surface Methodology (RSM) is applied to Arbequina EVOO, and the influence of oil quantity, extraction time, and extraction temperature on the total area and extraction of the major desirable compounds is analyzed. The experimental data are adequately fitted into second-order polynomial models with non-significant lack of fit (p > 0.05) and coefficients of determination (R and R -adjusted) higher than 0.88 and 0.78, respectively. A strong similarity is found between the predicted and experimental values. Furthermore, the surface plots show that the extraction of the volatile compounds is favored with increasing extraction temperature, time, and oil quantity. The extraction conditions to obtain the maximum response of “green” volatile compounds from Arbequina olive oil by HS-SPME placed in 50 mL vials are 4.6 g of oil, 43 min, and 59 C. Practical Applications: The findings of the present work show that specific conditions of HS-SPME/GC–MS affect the extraction of volatile compounds from cv. Arbequina olive oil and establish the optimum extraction parameters to more efficiently determine the global profile of this fraction, taking into account the organoleptic characteristics of the cultivar. The results also contribute to the knowledge of the volatile profile of the monovarietal olive oil cv. Arbequina, which is one of most cultivated and consumed types of olive oil worldwide.The authors are grateful to the CAPES Foundation − Ministry of Education, Brazil for the scholarship support provided to the first author. This work is part of the doctoral thesis of T.H.B., Ph.D. student from the Official Doctoral Program “Nutrition and Food Sciences” of the Granada University. The authors are also grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Program PT2020 for financial support to CIMO (UID/AGR/00690/2013). The authors dedicate this work to the memory of Carmen Cabrera Vique.Peer Reviewe

Effect of moisture on the characteristics of hazelnut kernel during storage.

The organoleptic and nutritional quality of hazelnuts during storage can be greatly influenced by environment conditions. The aim of this study was to evaluate the effect of moisture on the physicochemical characteristics of hazelnuts kernel when stored under controlled conditions of temperature (25 °C) and different relative humidity (57.6, 68.9, and 78.6%). To obtain these relative humidities (RH) in the hazelnut storage, the respective salts NaBr, KI, and NH4Cl were used. The following properties were evaluated: moisture, water activity (aw), color, texture, and fat oxidative stability, both in the initial sample and after 1, 2, 4 and 6 months of storage. The results showed that there was a progressive increase over time in moisture and water activity, with growth being most pronounced from 4 months onwards. Moreover, hazelnuts stored at a RH of 78.6%, at 6 months of storage were completely spoiled, making the evaluation of these hazelnuts impossible. The hardness and friability of the hazelnuts decreased over the storage time, with the differences being more pronounced for the samples subjected to higher RH. In general, the color of the skin and core of the fruits varied little over the storage time for all parameters evaluated (L*, a*, b*) and for all RH conditions tested. The hazelnuts stored at a RH of 78,6% can only be preserved until the 4th month, but presenting low oxidative stability, being comparable to the hazelnuts with 6 months at RH of 57,6%, meaning that with a reduction of about 10% of RH it is possible to increase the oxidative stability about 2 months. Thus, it can be concluded that high relative humidities lead to a lower conservation of the quality characteristics of hazelnuts. Moreover, during the 6 months of storage the color of the fruits was little affected for the different RH tested, however all the other evaluated characteristics presented significant alterations, namely the texture and the oxidative stability of the fats.info:eu-repo/semantics/publishedVersio

Influence of different parameters on the characteristics of hazelnut (var. Grada de Viseu) grown in Portugal

"Grada de Viseu” is an indigenous hazelnut variety from Portugal, and it is considered by the producers as the most important variety in terms of production and productivity. Therefore, the aim of this study was to assess the in fl uence of harvest year ( 2017/2018/2019 ) and location of production ( Viseu/Faia ) on some physical and chemical properties of the variety “ Grada de Viseu, ”namely biometric parameters, colour, true and bulk densities, texture parameters ( hardness and friability ) , and chemical composition. The results showed that the fruitsof the samples harvested in 2019 had higher values of height, width, and thickness. Hazelnuts from 2018 had a clearer shell, independently of the location of production, but only the samples from Faia harvested in 2018 had a clearer kernel. As for the texture, the fruits grown in Viseu harvested in 2018 had a harder shell and was more resistant to fracture, whereas the sample from 2019 had a harder kernel. In all cases, fat was the major chemical component. The sample from 2019 had a water activity greater than 0.62, meaning that its stability was not guaranteed. “ Grada de Viseu ” from Faia in 2018 presented a higher induction period and, therefore, was the one with the highest oxidation stability. The year of production showed to be the best predictor for almost every chemical and biometric characteristics. In general, it was possible to verify that harvesting year and geographical location in fl uence hazelnut characteristics.info:eu-repo/semantics/publishedVersio