Leflunomide derivative FK778 inhibits production of antibodies in an experimental model of alloreactive T-B cell interaction

El pdf es el artículo en full text.-- et al.[Objectives]: The contribution of humoral immune response in allograft and xenograft rejection has been clearly demonstrated in recent years. For this reason, inhibition of alloantibody production has become essential in managing transplanted patients. Here, we assessed the effects of the leflunomide derivative FK778 (FK778) in the control of antibody production resulting from semiallogeneic cognate T-B-cell interactions. [Materials and Methods]: BALB/c mice were tolerized at birth with semiallogeneic spleen cells from (BALB/c × C57BL/6) F1 mice, with or without overexpression of human bcl-2 transgene in B cells. These tolerized mice were treated with different dosages of FK778, either from birth, or from the third week of age, when autoantibody production was detected. The production of autoantibodies, used as markers of semiallogeneic cognate T-B-cell interactions, was evaluated at different time points during drug administration or after the interruption of treatment. [Results]: FK778 treatment started at birth inhibited the production of semiallogeneic-driven antibodies in a dose-dependent manner. In addition, FK778 also reduced the levels of preformed circulating autoantibodies in adult mice, although the dosage required was 4 times higher than that used in neonates. However, the levels of IgG antibodies in these tolerized mice increased after FK778 withdrawal, indicating that FK778 failed to induce tolerance to semiallogeneic host CD4+ Th2 and/or donor B cells. [Conclusions]: Our results demonstrate the efficacy of FK778 in the control of antibody production resulting from semiallogeneic cognate T-B-cell interactions. © Başkent University 2009. All Rights Reserved.This work was supported by REDINREN RD06/0016 and PI070683 grants from the “Instituto de Salud Carlos III, Ministerio de Sanidad y Consumo” (Spain) to MA, by grant No. SAF2005-00811 from the “Ministerio de Educación y Ciencia,” and a grant from the “Fundación Ramón Areces” (Spain) to RM, by grants No. SAF2006-012520-C02-02 from the “Ministerio de Educación y Ciencia,” and grant No. API07/08 from the “Fundación Marqués de Valdecilla” (Spain) to JM, and MAR-B is the recipient of a postdoctoral fellowship from the “Instituto Reina Sofía de la Fundación Renal Iñigo Alvarez de Toledo,” Spain. IS is funded by the “Fundación Marqués de Valdecilla, API06/07” (Spain).Peer Reviewe

A DSP-BASED active contour model

In this paper a DSP-based active contour model for tracking of the endocardium in a sequence of echocardiographic images is presented. If a contour is available in the first frame of a sequence, the contours in the subsequent frames are segmented. Deformable active contours is a technique that combine geometry, physics and approximation theory in order to solve problems of fundamental importance to medical image analysis; such as segmentation, representation and matching of shapes, and the tracking of objects in movement. The procedure has been developed on a DSP processor using its hardware features. The results are illustrated using a sequence of four-chambers apical echocardiographic images

Reduction of the speckle noise in echocardiographic images by a cubic spline filter

One of the main problems to resolve in the processing of biomedical images is the reduction of noise. The problem is specially important if the noise has a multiplicative nature (speckle noise), for instance if the object of analysis is an ultrasonic image. In this report we carry out a review of techniques which can be used to reduce this type of noise on four-chamber view B-mode echocardiographic images in an appropriated way. Different ways of nonlinear filtering, adaptive techniques based on the statistical ordering and a cubic spline interpolation will be shown as suitable techniques for this objective but regarding quantitative and qualitative results we have obtained, we can confirm that a cubic spline filter is the most suitable filter that we have reviewed.This work has been supported by Fundación Séneca of Región de Murcia and Ministerio de Ciencia y Tecnología of Spain, under grants PB/63/FS/02 and TIC2003-09400- C04-02, respectively

Lo llaman Democracia, pero ¿a qué? Tres perspectivas sobre el significado de la Democracia en tiempos de crisis y protesta

Review-Essay sobre las siguientes obras: • COLECTIVO NOVECENTO (VV. AA), Lo llamaban democracia. De la crisis económica al cuestionamiento de un régimen político, Icaria, Barcelona, 2013, ps. 96 • MONEREO, Manuel, De la crisis a la Revolución Democrática, El Viejo Topo, Barcelona, 2013, ps. 175 • RODRÍGUEZ, Emmanuel, Hipótesis Democracia. Quince tesis para la revolución anunciada, Traficantes de sueños, Madrid, 2013, ps. 37

Efecto de una exposición aguda a una estimulación neuromuscular mecánica en el tiempo de respuesta electiva a pie parado en tiradores de esgrima

En este trabajo se aplicó un protocolo de exposición aguda a las vibraciones en competidores de esgrima de nivel nacional, para analizar el efecto producido sobre el sistema neuromuscular en el tiempo de respuesta electiva

Extracción de Cu(II) y Zn(II) con ácidos resínicos

Tesis-Universidad Complutense de Madrid, 1.985.Fac. de Ciencias QuímicasTRUEProQuestpu

FUSULINOIDEANS FROM THE PUENTELLÉS FORMATION (UPPER CARBONIFEROUS, NW SPAIN): DISCUSSION ON PHYLOGENY, PALEOECOLOGY AND PALEOBIOGEOGRAPHY

The Cantabrian Zone is the only area in Western Europe that contains marine successions of Kasimovian and Gzhelian (late Late Carboniferous, late Pennsylvanian) age. These successions yield fusulinoideans that are presently the subject of an in depth-study by members of a IUGS SCCS working group intending to find stratigraphic markers for the subdivision of the Carboniferous System. The youngest fusulinoidean faunas of the Cantabrian Zone are recorded in the Puentellés Formation, a succession of calcareous strata consisting of two members. The lower member is made up of reworked and re-sedimented carbonates, which form shallow turbidite deposits showing frequent lateral changes of facies, whereas the upper member corresponds to autochthonous limestones, mainly consisting of dark mudstones, skeletal wackestones and thick-bedded boundstones. Generally speaking, the lower member is late Kasimovian in age while the upper one is early Gzhelian. However, there are also a few successions of late Kasimovian age that show sedimentary facies similar to those of the upper member. The fusulinoidean assemblages from the lower member are dominated by elongated species of the genus Ferganites, which sometimes occur with Schubertella and Staffella species. Rauserites, Tumefactus, Jigulites, Quasifusulina, as well as certain Ferganites species showing obese shell, mainly appear in strata belonging to the upper member.Detailed analyses of the fusulinoideans from the Cantabrian Zone have allowed several questions related to their paleoecology, biostratigraphy, and paleobiogeography to be ascertained. First, it has been shown that the Ferganites accumulated in the strata of the lower member could have lived in near-shore and high-energy environments. The presence of some relevant forms (e. g. Rauserites cf. rossicus, and Jigulites sp.) allow a correlation between the Cantabrian successions and the standard stratigraphic units of the Russian Platform. Moreover, the composition of the fusulinoidean assemblages clearly shows the biogeographic affinities of the Cantabrian Zone with the Carnic Alps and the Central Asian regions. On the contrary, these assemblages differ greatly from those in other western Eurasian areas, such as the Russian Platform and the Donets Basin, suggesting that these areas were not well-connected with the Paleo-Tethys.   Some observations give rise to relevant questions on more general aspects dealing with the latest Carboniferous fusulinoideans while yielding, at the same time, preliminary data for solving them. This is the case of the phrenotheca, an inner partition existing in some fusulinoidean shells whose functional role is still uncertain. The abundance in the Cantabrian Zone strata of Tumefactus specimens showing phrenotheca provides information for reconstructing its geometry as well as enabling speculation on its likely function. Another problematic aspect concerns the origin and distribution of the genus Triticites. It is noteworthy that this genus seems to be absent from both the Cantabrian Zone and most Eurasian areas, bringing up the question of whether the American and the Eurasian Triticites really had a common ancestor. The possibilities involved are discussed here.&nbsp

Análisis de la influencia de la estimulación con vibraciones en la respuesta neuromuscular en competidores de esgrima

La esgrima es un deporte de combate en el que todas las acciones están dirigidas a conseguir el objetivo de la competición, que es el de tocar al contrario sin ser tocado, y para ello los tiradores/as se sirven de todas las herramientas, técnicas, tácticas y de condición física posibles a su alcance. La calidad de las acciones de los esgrimistas en competición dependerá fundamentalmente de dos de los sistemas involucrados en el proceso. Por un lado, la activación del sistema nervioso, y por otro lado la respuesta del sistema muscular. Una de las herramientas utilizadas en los últimos años para estimular el sistema neuromuscular, y que ha reportado en muchas investigaciones resultados positivos, ha sido la plataforma de vibraciones vertical. Por esto, decidimos llevar a cabo un estudio con deportistas de esgrima de competición con un protocolo de exposición aguda a las vibraciones, y medir el efecto producido por esta herramienta sobre el sistema neuromuscular a través del estudio de los cambios experimentados en los tiempos de reacción simple, tiempos de respuesta electiva y tiempos de movimiento, así como los efectos en la eficacia del tocado de esgrima analizados antes y después de recibir el estímulo vibratorio. El estudio se desarrolló con tiradores/as de competición de nivel nacional y pertenecientes al Centro de Tecnificación de la Federación de Esgrima de Castilla y León (n=38; Edad: 22 ±9,08). La muestra estaba compuesta por 12 mujeres y 26 hombres, de categorías, cadetes (13), júnior (12), y absolutos (13). El protocolo elegido fue realizado por cada participante en tres ocasiones, una inicial, otra tras pasar por una carga de estimulación en la plataforma de vibraciones, y una fase final, pasado un tiempo de recuperación de 10 minutos para comprobar el grado de disipación del efecto vibratorio. El estímulo elegido para la estimulación sobre la plataforma de vibraciones fue de una frecuencia de 50 Hz, durante un periodo de 60 segundos y con una amplitud de 4 mm. Los resultados se analizaron en función de las variables dependientes tiempo de reacción, tiempo de respuesta electiva, tiempo de movimiento, y precisión y eficacia. Estos datos se cruzaron con las variables independientes sexo, categoría, nivel deportivo y años de experiencia. El propósito del presente estudio fue el de analizar los efectos producidos por una intervención de estimulación neuromuscular mecánica en fase aguda en un grupo de tiradores/as de esgrima de competición. Los resultados mostraron que la carga de estimulación neuromuscular mecánica (ENM) aplicada en nuestro estudio provocó un discreto efecto en la respuesta neuromuscular de los tiradores participantes en la investigación. Se encontraron efectos significativos provocados por el estímulo vibratorio en las siguientes variables: en el tiempo de reacción simple se registró una mejora del 8,1%, en el tiempo de respuesta electiva a pie parado un 10%, en la precisión a pie parado un 7%, y en la eficacia a pie parado un 18,5%. Igualmente se observaron ligeras diferencias por sexos, encontrando un efecto de mayor tamaño en el grupo femenino. Es necesario resaltar que las características particulares de la muestra parecen haber influido en los resultados encontrados de forma importante. Por último, debemos destacar que el efecto residual producido por la estimulación aplicada en nuestra investigación en algún caso superó los diez minutos, ya que se hallaron efectos positivos de la estimulación neuromuscular mecánica en varios de los registros finales como hemos visto. ABSTRACT Fencing is a combat sport in which all actions are aimed at achieving the objective of the competition, which is that of touching the opponent without being touched and for this the fencers make use of all the tools, techniques, tactics and physical condition possible at their disposal. The quality of the fencers´ actions in competition will depend primarily on two of the systems involved in the process: on the one hand, the activation of the nervous system and, on the other, the response of the muscular system. One of the tools recently used to stimulate the neuromuscular system, and which has produced positive results in many research studies, has been the vertical vibration platform. Therefore, we decided to conduct a study with competition fencers with a protocol of acute exposure to vibration and to measure the effect produced by this tool on the neuromuscular system by means of the study of changes experienced in simple reaction times, the elective response times and movement times, as well as the effects on the efficacy of the fencing touch analyzed before and after receiving the vibratory stimulus. The study was conducted with fencers in national competitions and belonging to the Technification Center of the Fencing Federation of Castilla y León (n = 38), with a mean age of 22 years (SD 9.08). The sample was composed of 12 women and 26 men, by categories cadets (13), juniors (12), and seniors (13). The protocol chosen was performed by each participant on three occasions: an initial one, another after going through a stimulation load on the vibration platform, and a final phase, after a 10 minute recovery time to assess the degree of dissipation of the vibratory effect. The stimulus chosen on the vibration platform was a frequency of 50 Hz, for a period of 60 seconds and an amplitude of 4 mm. The results were analyzed according to the following dependent variables: the reaction time, the response time, the accuracy and the efficiency. This data was crossed with the independent variables: gender, category, sporting level and the years of experience. The purpose of this study was analyze the effects of neuromuscular stimulation intervention in a group of fencing competition shooters. The results showed that the mechanical neuromuscular stimulation (MNS) load applied in our study led to a modest effect on the neuromuscular response of the fencers involved in the research. Significant effects were found caused by the vibratory stimulus on the simple reaction time 8,1%, elective response time 10%, as well as the accuracy 7%, and efficiency 18,5%. We should also point out that slight differences by gender were observed, finding a greater effect in females. It should be emphasized that the specific characteristics of the sample appear to have significantly influenced the results. Finally, we should note that the residual effect produced by the stimulation applied in our research in some cases exceeded ten minutes, given that positive effects of the mechanical neuromuscular stimulation were found in several of the final scores

An automatic welding defects classifier system

Radiographic inspection is a well-established testing method to detect weld defects. However, interpretation of radiographic films is a difficult task. The reliability of such interpretation and the expense of training suitable experts have allowed that the efforts being made towards automation in this field. In this paper, we describe an automatic detection system to recognise welding defects in radiographic images. In a first stage, image processing techniques, including noise reduction, contrast enhancement, thresholding and labelling were implemented to help in the recognition of weld regions and the detection of weld defects. In a second stage, a set of geometrical features was proposed and extracted between defect candidates. In a third stage, an artificial neural network for weld defect classification was used under three regularisation process with different architectures. For the input layer, the principal component analysis technique was used in order to reduce the number of feature variables; and, for the hidden layer, a different number of neurons was used in the aim to give better performance for defect classification in both cases