Carp edema virus in ornamental fish farming in India: A potential threat to koi carps but not to co-cultured Indian major carp or goldfish

254-262Koi sleepy disease caused by carp edema virus (CEV) of pox virus is a potential carp killer and poses a constant threat to co-cultured fish. Only limited studies are available on its occurrence from the eastern part of India. Here, we report a large-scale mortality of koi carps (Cyprinuscarpio koi)in ornamental fish farm of Choudwar, Cuttack, Odisha. The fish showed clinical signs of ulcers on body, patchy haemorrhages, massive necrosis of gills, and observed sleeping at the bottom of tanks before death. However, the goldfish maintained in the same tanks did not show any clinical signs or mortality. We noticed Trichodina infestation in the gills of affected animals and secondary bacterium Aeromonashydrophila in kidney tissue. The gills, liver and kidneys of koi carp were found positive for carp edema virus (CEV) (Poxviridae) in PCR followed by sequencing and blast search, and phylogenetic analysis. The damages to the gills and kidney along with the presence of intracytoplasmic eosinophilic inclusions in gill epithelial cells upon histopathology suggested viral association. Experimental challenge with CEV failed to reproduce the disease in Indian major carp and goldfish, the commonly co-cultured fish species with koi carp, though, the disease was reproduced in koi carp. To our best knowledge, it is the first report of CEV infection from the farm of the eastern part of the country. Another mortality incidence in ornamental shop retailer in the same locality also revealed the presence of CEV in koi carp and pointed possibly towards its wide spread. Observations from this study suggest the need for CEV disease surveillance in the country

Fragility curves for special truss moment frame with single and multiple vierendeel special segment

[EN] Special Truss Moment frame (STMF) is an open web truss moment frame, which dissipates the input seismic energy through a well-defined ductile special segment located near the mid-span of truss while other members of truss outside the special segment and columns are designed to remain elastic. In this paper, the performance and the fragility curve of STMFs consisting single and multiple vierendeel panels in the special segment are investigated. The seismic response of nine-story having the length to depth ratio of special segment 2.5 is considered to develop the fragility curve. The seismic response of each building was recorded by performing nonlinear incremental dynamic analyses. Each archetype modelled in nonlinear analysis program PERFORM-3D to carry out IDA under a suit of forty-four real Far Field ground motion records. Fragility curves were developed for these structures and the probability of exceedance at immediate occupancy (IO) level, Life safety (LS) level and Collapse performance (CP) level was assessed for two level of hazards, DBE level (10% probability of exceedance in 50 years) and MCE level (2% probability of exceedance in 50 years). For DBE level earthquake intensity, the probability of exceedance for the CP performance level of STMF building for both structure is marginal while at MCE level the probability of exceedance at CP performance level is 71% and 45% for single and multiple panels respectively.Kumar, R.; Sahoo, D.; Gupta, A. (2018). Fragility curves for special truss moment frame with single and multiple vierendeel special segment. En Proceedings of the 12th International Conference on Advances in Steel-Concrete Composite Structures. ASCCS 2018. Editorial Universitat Politècnica de València. 739-746. https://doi.org/10.4995/ASCCS2018.2018.7248OCS73974

Carp edema virus in ornamental fish farming in India: A potential threat to koi carps but not to co-cultured Indian major carp or goldfish

254-262Koi sleepy disease caused by carp edema virus (CEV) of pox virus is a potential carp killer and poses a constant threat to co-cultured fish. Only limited studies are available on its occurrence from the eastern part of India. Here, we report a large-scale mortality of koi carps (Cyprinuscarpio koi)in ornamental fish farm of Choudwar, Cuttack, Odisha. The fish showed clinical signs of ulcers on body, patchy haemorrhages, massive necrosis of gills, and observed sleeping at the bottom of tanks before death. However, the goldfish maintained in the same tanks did not show any clinical signs or mortality. We noticed Trichodina infestation in the gills of affected animals and secondary bacterium Aeromonashydrophila in kidney tissue. The gills, liver and kidneys of koi carp were found positive for carp edema virus (CEV) (Poxviridae) in PCR followed by sequencing and blast search, and phylogenetic analysis. The damages to the gills and kidney along with the presence of intracytoplasmic eosinophilic inclusions in gill epithelial cells upon histopathology suggested viral association. Experimental challenge with CEV failed to reproduce the disease in Indian major carp and goldfish, the commonly co-cultured fish species with koi carp, though, the disease was reproduced in koi carp. To our best knowledge, it is the first report of CEV infection from the farm of the eastern part of the country. Another mortality incidence in ornamental shop retailer in the same locality also revealed the presence of CEV in koi carp and pointed possibly towards its wide spread. Observations from this study suggest the need for CEV disease surveillance in the country

Artificial Neural Network based Body Posture Classification from EMG signal analysis

 This paper deals with the body posture Classification from EMG signal analysis using artificial neural network (ANN). The various statistical features extracted from each EMG signal corresponding to different muscles associated with the different body postures are framed using LABVIEW software. Further-more, these features are taken as the input towards the ANN classifier and thus the corresponding output for the respective classifier predicts the postures like Bowing, Handshaking, and Hugging. The performance of the classifier is determined by the classification rate (CR). The outcome of result indicates that the CR of Multilayer Feed Forward Neural Network (MFNN) type of ANN is rounded up to a percentage of 71.02%

Statistical optimization for lipase production from solid waste of vegetable oil industry

<p>The production of biofuel using thermostable bacterial lipase from hot spring bacteria out of low-cost agricultural residue olive oil cake is reported in the present paper. Using a lipase enzyme from <i>Bacillus licheniformis</i>, a 66.5% yield of methyl esters was obtained. Optimum parameters were determined, with maximum production of lipase at a pH of 8.2, temperature 50.8°C, moisture content of 55.7%, and biosurfactant content of 1.693 mg. The contour plots and 3D surface responses depict the significant interaction of pH and moisture content with biosurfactant during lipase production. Chromatographic analysis of the lipase transesterification product was methyl esters, from kitchen waste oil under optimized conditions, generated methyl palmitate, methyl stearate, methyl oleate, and methyl linoleate.</p