Identification and characterization of a PKC-like kinase in potato and its role in the activation of the defense response gene PR-10a

Thèse numérisée par la Direction des bibliothèques de l'Université de Montréal

The Role of Supply Chain Integration on Green Practices and Performance in a Supply Chain Context. A Conceptual Approach to Future Research

The purpose of this paper is to investigate the current research relationship between green supply chain management practices, supply chain integration and the manufacturing firms performances in Malaysia. Also, to improves the conceptual knowledge by compare and contrast the relationship between each dimension. The study thoroughly analyzes, reviews and explains each dimension in detail and their relationships from various previous literature perspectives. The paper is one of the first to identify and discuss conceptually the use of green supply chain management practices and supply chain integration in measuring the manufacturing performance in a single setting in a developing country

Making sense of methods – a conversation about qualitative research in library and information studies

Currently there is an embarrassment of riches with regards to the range of research methods appropriate for library and information studies [LIS]; including qualitative and quantitative methods as well as ‘mixed methods’. All of this provides a rich body of resources for researchers, but this abundance also has a downside since it can also result in confusion and perplexity amongst researchers as they plan their investigative studies. Contributions such as this special issue are welcome opportunities to resolve and ameliorate this situation, and so in our contribution we seek to address some of these issues in the form of an interchange between two researchers with interests that include, but are not limited to, research in LIS. Between us we have a wide range of publications, as well as 80 plus PhD completions, many of which fall under the heading of LIS – broadly conceived. In particular we would claim specific expertise in Grounded Theory [Bryant] and Action Research [Abbott-Halpin]. Our aim is to seek clarification of some of the key methodological issues; although we realize that this is unlikely to provide any definitive outcome, it may assist those seeking guidance on these matters

Static Pipeline Network Performance Optimisation Using Dual Interleave Routing Algorithm

In the recent years, there is an increasing demand on multi-hop wireless sensor networks (WSN) especially for remote condition and integrity monitoring of oil and gas pipelines. The sensing points are connected through WSN points, known as a wireless communication medium, between the remotely measured locations on a pipeline and a centralised monitoring station, located some distance away. Generally, WSN deployment on a multi-hop linear topology has critical factors that contribute towards overall degrading of network performance proportional to the number of nodes. This is especially true in highly dense networks. In general, such a drawback contributes towards poor network reliability, low network capacity, high latency, and inequality with snowballing effect, increasing in the direction of the destination node. This paper introduces the Dual Interleaving Linear Static Routing (DI-LSR) for a multi-hop linear network with high reliability and efficiency to significantly enhance the overall network performance of a pipeline network. The DI-LSR was tested and analysed according to IEEE 802.11 standard in a various simulation environment for future real-time deployment in a pipeline network

TCP Performance And Throughput Fairness Optimization In A Multi-Hop Pipeline Network

Node starvation wireless sensor network (WSN) is a critical factor that affects the overall performance in a typical multi-hop linear network especially in an extensive scale network. The unfairness of sharing network resources with all source nodes in a multi-hop linear network amplifies the node starvation that often results in passive nodes in a network. This factor becomes critical with the increasing network density, aggressive data transfer, single destination node and inadequate data scheduling. This paper highlights the Delayed acknowledgement timeout for flat one-tier throughput critical application model (DAT-FTCAM) a mathematical fairness model that ensure maximum throughput fairness for pipeline network scenario. The DAT-FTCAM enables the users to calculate the maximum delayed acknowledgement timeout for transmission control protocol (TCP) proportional to the travel time or difference between a source and a destination node. The implementation of DAT-FTCAM technique with modified TCP parameters on NS2 has revealed a network fairness index of above 0.99 with optimum network performance in a scalable pipeline network. The DAT-FTCAM decreases data packet collision and eliminates passive nodes in a pipeline network with optimum throughput fairnes

Tri6 Is a Global Transcription Regulator in the Phytopathogen Fusarium graminearum

In F. graminearum, the transcriptional regulator Tri6 is encoded within the trichothecene gene cluster and regulates genes involved in the biosynthesis of the secondary metabolite deoxynivalenol (DON). The Tri6 protein with its Cys2His2 zinc-finger may also conform to the class of global transcription regulators. This class of global transcriptional regulators mediate various environmental cues and generally responds to the demands of cellular metabolism. To address this issue directly, we sought to find gene targets of Tri6 in F. graminearum grown in optimal nutrient conditions. Chromatin immunoprecipitation followed by Illumina sequencing (ChIP-Seq) revealed that in addition to identifying six genes within the trichothecene gene cluster, Tri1, Tri3, Tri6, Tri7, Tri12 and Tri14, the ChIP-Seq also identified 192 additional targets potentially regulated by Tri6. Functional classification revealed that, among the annotated genes, ∼40% are associated with cellular metabolism and transport and the rest of the target genes fall into the category of signal transduction and gene expression regulation. ChIP-Seq data also revealed Tri6 has the highest affinity toward its own promoter, suggesting that this gene could be subject to self-regulation. Electro mobility shift assays (EMSA) performed on the promoter of Tri6 with purified Tri6 protein identified a minimum binding motif of GTGA repeats as a consensus sequence. Finally, expression profiling of F. graminearum grown under nitrogen-limiting conditions revealed that 49 out of 198 target genes are differentially regulated by Tri6. The identification of potential new targets together with deciphering novel binding sites for Tri6, casts new light into the role of this transcriptional regulator in the overall growth and development of F. graminearum

On the stability and uniqueness of the flow of a fluid through a porous medium

© 2016, The Author(s). In this short note, we study the stability of flows of a fluid through porous media that satisfies a generalization of Brinkman’s equation to include inertial effects. Such flows could have relevance to enhanced oil recovery and also to the flow of dense liquids through porous media. In any event, one cannot ignore the fact that flows through porous media are inherently unsteady, and thus, at least a part of the inertial term needs to be retained in many situations. We study the stability of the rest state and find it to be asymptotically stable. Next, we study the stability of a base flow and find that the flow is asymptotically stable, provided the base flow is sufficiently slow. Finally, we establish results concerning the uniqueness of the flow under appropriate conditions, and present some corresponding numerical results

Genomic Identification of the TOR Signaling Pathway as a Target of the Plant Alkaloid Antofine in the Phytopathogen Fusarium graminearum

Antofine, a phenanthroindolizidine alkaloid, is a bioactive natural product isolated from milkweeds that exhibits numerous biological activities, including anticancer, antimicrobial, antiviral, and anti-inflammatory properties. However, the direct targets and mode of action of antofine have not been determined. In this report, we show that antofine displays antifungal properties against the phytopathogen Fusarium graminearum, the cause of Fusarium head blight disease (FHB). FHB does devastating damage to agriculture, causing billions of dollars in economic losses annually. We therefore sought to understand the mode of action of antofine in F. graminearum using insights from yeast chemical genomic screens. We used haploinsufficiency profiling (HIP) to identify putative targets of antofine in yeast and identified three candidate targets, two of which had homologs in F. graminearum The Fusarium homologues of two targets, glutamate dehydrogenase (FgGDH) and resistance to rapamycin deletion 2 (FgRRD2), can bind antofine. Of the two genes, only the Fgrrd2 knockout displayed a los

MUM ENHANCERS are important for seed coat mucilage production and mucilage secretory cell differentiation in Arabidopsis thaliana

Pollination triggers not only embryo development but also the differentiation of the ovule integuments to form a specialized seed coat. The mucilage secretory cells of the Arabidopsis thaliana seed coat undergo a complex differentiation process in which cell growth is followed by the synthesis and secretion of pectinaceous mucilage. A number of genes have been identified affecting mucilage secretory cell differentiation, including MUCILAGE-MODIFIED4 (MUM4). mum4 mutants produce a reduced amount of mucilage and cloning of MUM4 revealed that it encodes a UDP-L-rhamnose synthase that is developmentally up-regulated to provide rhamnose for mucilage pectin synthesis. To identify additional genes acting in mucilage synthesis and secretion, a screen for enhancers of the mum4 phenotype was performed. Eight mum enhancers (men) have been identified, two of which result from defects in known mucilage secretory cell genes (MUM2 and MYB61). Our results show that, in a mum4 background, mutations in MEN1, MEN4, and MEN5 lead to further reductions in mucilage compared to mum4 single mutants, suggesting that they are involved in mucilage synthesis or secretion. Conversely, mutations in MEN2 and MEN6 appear to affect mucilage release rather than quantity. With the exception of men4, whose single mutant exhibits reduced mucilage, none of these genes have a single mutant phenotype, suggesting that they would not have been identified outside the compromised mum4 background