Assessment and comparison of the Marennes-Oléron Bay (France) and Carlingford Lough (Ireland) carrying capacity with ecosystem models

Based on the individual growth, food limitation, population renewal through seeding, and individual marketable size, a theoretical model of the cultured species population dynamics was used to assess the carrying capacity of an ecosystem. It gave a domeshape curve relating the annual production and the standing stock under the assumption of individual growth limited by the available food in an ecosystem. It also showed the influence of mortality rate and marketable size on this curve and was introduced as a means to explore the global properties resulting from the interactions between the ecophysiology of the reared species and the environment at the ecosystem level. In a second step, an ecosystem model was built to assess the carrying capacity of MarennesOl ´eron bay, the most important shellfish culture site in France, with a standing stock of Crassostrea gigas around 100 000 tonnes fresh weight (FW) and an annual production of 30 000 tonnes FW. The ecosystem model focused on the oyster growth rate and considered the interaction between food availability, residence time of the water, oyster ecophysiology and number of individuals. It included a spatial discretization of the bay (box design) based on a hydrodynamicmodel, and the nitrogen or carbon cycling between phytoplankton, cultured oysters, and detritus. From simulations of the oyster growth with different seeding values, a curve relating the total annual production and the standing stock was obtained. This curve exhibited a dome shape with a maximum production corresponding to an optimum standing stock. The model predicted amaximum annual production of 45 000 tonnes FWfor a standing stock around 115 000 tonnes FW. The prediction confirmed some results obtained empirically in the case of MarennesOl ´eron bay and the results of the theoretical model. Results were compared with those obtained in Carlingford Lough (Ireland) using a similar ecosystem model. Carlingford Lough is a small intertidal bay where the same species is cultured at a reduced scale, with current biomass less than 500 tonnes FW. The model showed that the standing stock can be increased from 200 tonnes FW to approximately 1500 tonnes FW before any decrease of the production

Measuring the Surface Thermal Structure of the Sea off Brittany by Ship and Aircraft

During the months of September and October 1975 the “Laboratoire d ’Océanographie Physique de l’Université de Bretagne Occidentale” (LOP) and the “Laboratoire de Météorologie Dynamique du C.N.R.S.” (LMD) made a joint study of surface temperatures and their variability over the continental plateau off the westernmost point of Brittany. Between 18 September and 28 October scientists from the LOP made several trips aboard the oceanographic vessel Capricorne operating a sonde for in-situ T.S.D. measurements. The LMD utilized an Aries radiometer which they themselves had designed and perfected. This was installed aboard a DC-3 aircraft that also carried pressure, humidity and temperature sensors. Our two objectives were as follows : 1. To compare the results obtained by two different techniques; 2. To obtain details of certain aspects of the microstructure of the sea surface, in particular over a frontal region. This paper thus proposes : a) To compare the results of the bathysonde measurements carried out by the LOP on Capricorne with those of a team from the “ Centre Océanologique de Bretagne" (COB) embarked in the oceanographic vessel Cry os from 16 to 29 September 1975. b) To present charts derived from radiometric measurements and to compare these charts with previous ones. c) To present aspects of the fine thermal structure at the surface from a certain number of radial passes with the DC-3

Assessment and comparison of the Marennes-Oléron Bay (France) and Carlingford Lough (Ireland) carrying capacity with ecosystem models

Based on the individual growth, food limitation, population renewal through seeding, and individual marketable size, a theoretical model of the cultured species population dynamics was used to assess the carrying capacity of an ecosystem. It gave a domeshape curve relating the annual production and the standing stock under the assumption of individual growth limited by the available food in an ecosystem. It also showed the influence of mortality rate and marketable size on this curve and was introduced as a means to explore the global properties resulting from the interactions between the ecophysiology of the reared species and the environment at the ecosystem level. In a second step, an ecosystem model was built to assess the carrying capacity of MarennesOl ´eron bay, the most important shellfish culture site in France, with a standing stock of Crassostrea gigas around 100 000 tonnes fresh weight (FW) and an annual production of 30 000 tonnes FW. The ecosystem model focused on the oyster growth rate and considered the interaction between food availability, residence time of the water, oyster ecophysiology and number of individuals. It included a spatial discretization of the bay (box design) based on a hydrodynamicmodel, and the nitrogen or carbon cycling between phytoplankton, cultured oysters, and detritus. From simulations of the oyster growth with different seeding values, a curve relating the total annual production and the standing stock was obtained. This curve exhibited a dome shape with a maximum production corresponding to an optimum standing stock. The model predicted amaximum annual production of 45 000 tonnes FWfor a standing stock around 115 000 tonnes FW. The prediction confirmed some results obtained empirically in the case of MarennesOl ´eron bay and the results of the theoretical model. Results were compared with those obtained in Carlingford Lough (Ireland) using a similar ecosystem model. Carlingford Lough is a small intertidal bay where the same species is cultured at a reduced scale, with current biomass less than 500 tonnes FW. The model showed that the standing stock can be increased from 200 tonnes FW to approximately 1500 tonnes FW before any decrease of the production

Enantiospecific pharmacokinetics of intravenous dexmedetomidine in beagles

The goal of this study was to investigate the pharmacokinetic (PK) behaviour of dexmedetomidine in dogs administered as a pure enantiomer versus as part of a racemic mixture. Eight unmedicated intact purpose-bread beagles were included. Two intravenous treatments of either medetomidine or dexmedetomidine were administered at 10- to 14-day intervals. Atipamezole or saline solution was administered intramuscularly 45 min later. Venous blood samples were collected into EDTA collection tubes, and the quantification of dexmedetomidine and levomedetomidine was performed by chiral LC–MS/MS. All dogs appeared sedated after each treatment without complication. Plasma concentrations of levomedetomidine were measured only in the racemic group and were 51.4% (51.4%–56.1%) lower than dexmedetomidine. Non-compartmental analysis (NCA) was performed for both drugs, while dexmedetomidine data were further described using a population pharmacokinetic approach. A standard two-compartment mammillary model with linear elimination with combined additive and multiplicative error model for residual unexplained variability was established for dexmedetomidine. An exponential model was finally retained to describe inter-individual variability on parameters of clearance (Cl1) and central and peripheral volumes of distribution (V1, V2). No effect of occurrence, levomedetomidine or atipamezole could be observed on dexmedetomidine PK parameters. Dexmedetomidine did not undergo significantly different PK when administered alone or as part of the racemic mixture in otherwise unmedicated dogs

A Study of Nuclear Transcription Factor-Kappa B in Childhood Autism

BACKGROUND: Several children with autism show regression in language and social development while maintaining normal motor milestones. A clear period of normal development followed by regression and subsequent improvement with treatment, suggests a multifactorial etiology. The role of inflammation in autism is now a major area of study. Viral and bacterial infections, hypoxia, or medication could affect both foetus and infant. These stressors could upregulate transcription factors like nuclear factor kappa B (NF-κB), a master switch for many genes including some implicated in autism like tumor necrosis factor (TNF). On this hypothesis, it was proposed to determine NF-κB in children with autism. METHODS: Peripheral blood samples of 67 children with autism and 29 control children were evaluated for NF-κB using electrophoretic mobility shift assay (EMSA). A phosphor imaging technique was used to quantify values. The fold increase over the control sample was calculated and statistical analysis was carried out using SPSS 15. RESULTS: We have noted significant increase in NF-κB DNA binding activity in peripheral blood samples of children with autism. When the fold increase of NF-κB in cases (n = 67) was compared with that of controls (n = 29), there was a significant difference (3.14 vs. 1.40, respectively; p<0.02). CONCLUSION: This finding has immense value in understanding many of the known biochemical changes reported in autism. As NF-κB is a response to stressors of several kinds and a master switch for many genes, autism may then arise at least in part from an NF-κB pathway gone awry

Intramolecular Epistasis and the Evolution of a New Enzymatic Function

Atrazine chlorohydrolase (AtzA) and its close relative melamine deaminase (TriA) differ by just nine amino acid substitutions but have distinct catalytic activities. Together, they offer an informative model system to study the molecular processes that underpin the emergence of new enzymatic function. Here we have constructed the potential evolutionary trajectories between AtzA and TriA, and characterized the catalytic activities and biophysical properties of the intermediates along those trajectories. The order in which the nine amino acid substitutions that separate the enzymes could be introduced to either enzyme, while maintaining significant catalytic activity, was dictated by epistatic interactions, principally between three amino acids within the active site: namely, S331C, N328D and F84L. The mechanistic basis for the epistatic relationships is consistent with a model for the catalytic mechanisms in which protonation is required for hydrolysis of melamine, but not atrazine

Influence of Aqueous Solubility of Various VOCs on Their Photocatalytic Degradation

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