233 research outputs found
The effects of stretching with cryotherapy, stretching with heat and stretching alone on hamstring flexibility in physically active females
Revealing the evolutionary history and epidemiological dynamics of emerging RNA viral pathogens
Fast-evolving RNA viruses are a leading cause of morbidity and mortality among human
and animal populations, contributing significantly to both global health and economic
burden. The advent and revolution of high-throughput sequencing has empowered
phylogenetic analyses with increasing amounts of temporally and spatially sampled
viral data. Moreover, the parallel advancement in molecular evolution and phylogenetic
methods has provided investigators with a unique opportunity to gain detailed
insight into the evolutionary and epidemiological dynamics of emerging viral pathogens.
Using state-of-the-art statistical approaches, this thesis addresses some of the important
but controversial questions in viral emergence. Chapter 2 introduces a new
framework to quantify and investigate reassortment events in influenza A viruses. By
developing a computationally efficient algorithm to calculate the largest common subtree
for a pair of tree sets, which are estimated from diffe rent parts of the genome for
the same taxa set, the level of phylogenetic incongruency due to reassortment can be
appropriately ascertained. Chapters 3, 4 and 5 investigate the evolutionary origins of
three diff erent viruses: the novel emergence and cross-species transmission of SARSCoV,
the genesis and dissemination of the unique HCV circulating recombinant form,
and the ancient divergence of all influenza viruses, respectively. Moreover, Chapter 4
presents an improved statistical framework, which provides more precise evolutionary
estimates, by utilizing the hierarchical bayes approach to investigate recombination
events in emerging RNA viruses. The last empirical study, presented in Chapter 6, applies
the recently developed Bayesian phylogeography models to a large viral sequence
dataset sampled from southern Viet Nam to examine the fine-scale spatiotemporal dynamics
of endemic dengue in Southeast Asia.
The work presented here reflects both the advancements made in sequencing technology
and statistical phylogenetics, along with some of the challenges that remain in
studying the emergence of fast-evolving RNA viruses. This thesis proposes new and
improved solutions to these evolutionary problems, such as incorporating non-vertical
evolution (i.e. homologous recombination and reassortment) into the phylodynamic
framework, with the aim of facilitating future investigations of emerging viral diseases
A de novo approach to inferring within-host fitness effects during untreated HIV-1 infection
Funder: Isaac Newton Trust; funder-id: http://dx.doi.org/10.13039/501100004815Funder: Li Ka Shing Foundation; funder-id: http://dx.doi.org/10.13039/100007421Funder: Division of Intramural Research, National Institute of Allergy and Infectious Diseases; funder-id: http://dx.doi.org/10.13039/100006492Funder: Helsingin Yliopisto; funder-id: http://dx.doi.org/10.13039/100007797In the absence of effective antiviral therapy, HIV-1 evolves in response to the within-host environment, of which the immune system is an important aspect. During the earliest stages of infection, this process of evolution is very rapid, driven by a small number of CTL escape mutations. As the infection progresses, immune escape variants evolve under reduced magnitudes of selection, while competition between an increasing number of polymorphic alleles (i.e., clonal interference) makes it difficult to quantify the magnitude of selection acting upon specific variant alleles. To tackle this complex problem, we developed a novel multi-locus inference method to evaluate the role of selection during the chronic stage of within-host infection. We applied this method to targeted sequence data from the p24 and gp41 regions of HIV-1 collected from 34 patients with long-term untreated HIV-1 infection. We identify a broad distribution of beneficial fitness effects during infection, with a small number of variants evolving under strong selection and very many variants evolving under weaker selection. The uniquely large number of infections analysed granted a previously unparalleled statistical power to identify loci at which selection could be inferred to act with statistical confidence. Our model makes no prior assumptions about the nature of alleles under selection, such that any synonymous or non-synonymous variant may be inferred to evolve under selection. However, the majority of variants inferred with confidence to be under selection were non-synonymous in nature, and in most cases were have previously been associated with either CTL escape in p24 or neutralising antibody escape in gp41. We also identified a putative new CTL escape site (residue 286 in gag), and a region of gp41 (including residues 644, 648, 655 in env) likely to be associated with immune escape. Sites inferred to be under selection in multiple hosts have high within-host and between-host diversity although not all sites with high between-host diversity were inferred to be under selection at the within-host level. Our identification of selection at sites associated with resistance to broadly neutralising antibodies (bNAbs) highlights the need to fully understand the role of selection in untreated individuals when designing bNAb based therapies
Pathogen genomics and One Health: a scoping review of current practices in zoonotic disease research
Objectives:
Whole-genome sequencing has revolutionised the field of infectious disease surveillance, enabling near real-time detection of pathogens and tracking how infections may spread. Our study aimed to characterise genomic applications to cross-domain zoonotic pathogen transmission at the human-animal and/or human-environment interfaces.
Methods:
We performed a scoping review of studies that have applied genomic epidemiology to zoonotic disease transmission across One Health domains (human, animal, and environment). We identified 114 records published between 2005 and 2022 which reported multi-domain genomic data of zoonotic pathogens integrated into phylogenetic models.
Results:
Most studies investigated bacterial pathogens, highlighting key knowledge gaps for other zoonotic agents, particularly arboviruses. Sampling and sequencing efforts varied greatly across domains: the median number and range of pathogen genomes analysed were highest for humans (23; 1-29,586) and lowest for the environment domain (13; 1-956). Genomics was used to track zoonotic disease outbreaks and cross-domain transmission, to improve pathogen surveillance, and to disentangle evolutionary dynamics driving lineage diversification and virulence.
Conclusions:
Our study highlights current practices and knowledge gaps to guide future study designs and genomic applications to multi-domain and cross-species transmission of zoonoses, with the potential to identify key infection sources and inform interventions for local and global health security
Molecular evolution, diversity and adaptation of H7N9 viruses in China during 2013-2017
The substantial increase in prevalence and emergence of antigenically divergent or highly pathogenic influenza A(H7N9) viruses during 2016–17 raises concerns about the epizootic potential of these viruses. We investigated the evolution and adaptation of H7N9 viruses by analyzing available data and newly generated virus sequences isolated in Guangdong Province, China, during 2015–2017. Phylogenetic analyses showed that circulating H7N9 viruses belong to distinct lineages with differing spatial distributions. Hemagglutination inhibition assays performed on serum samples from patients infected with these viruses identified 3 antigenic clusters for 16 strains of different virus lineages. We used ancestral sequence reconstruction to identify parallel amino acid changes on multiple separate lineages. We inferred that mutations in hemagglutinin occur primarily at sites involved in receptor recognition or antigenicity. Our results indicate that highly pathogenic strains likely emerged from viruses circulating in eastern Guangdong Province during March 2016 and are associated with a high rate of adaptive molecular evolution
A Molecular-Level Account of the Antigenic Hantaviral Surface
Hantaviruses, a geographically diverse group of zoonotic pathogens, initiate cell infection through the concerted action of Gn and Gc viral surface glycoproteins. Here, we describe the high-resolution crystal structure of the antigenic ectodomain of Gn from Puumala hantavirus (PUUV), a causative agent of hemorrhagic fever with renal syndrome. Fitting of PUUV Gn into an electron cryomicroscopy reconstruction of intact Gn-Gc spike complexes from the closely related but non-pathogenic Tula hantavirus localized Gn tetramers to the membrane-distal surface of the virion. The accuracy of the fitting was corroborated by epitope mapping and genetic analysis of available PUUV sequences. Interestingly, Gn exhibits greater non-synonymous sequence diversity than the less accessible Gc, supporting a role of the host humoral immune response in exerting selective pressure on the virus surface. The fold of PUUV Gn is likely to be widely conserved across hantaviruses.Peer reviewe
A Molecular-Level Account of the Antigenic Hantaviral Surface
Hantaviruses, a geographically diverse group of zoonotic pathogens, initiate cell infection through the concerted action of Gn and Gc viral surface glycoproteins. Here, we describe the high-resolution crystal structure of the antigenic ectodomain of Gn from Puumala hantavirus (PUUV), a causative agent of hemorrhagic fever with renal syndrome. Fitting of PUUV Gn into an electron cryomicroscopy reconstruction of intact Gn-Gc spike complexes from the closely related but non-pathogenic Tula hantavirus localized Gn tetramers to the membrane-distal surface of the virion. The accuracy of the fitting was corroborated by epitope mapping and genetic analysis of available PUUV sequences. Interestingly, Gn exhibits greater non-synonymous sequence diversity than the less accessible Gc, supporting a role of the host humoral immune response in exerting selective pressure on the virus surface. The fold of PUUV Gn is likely to be widely conserved across hantaviruses.Peer reviewe
No evidence for local adaptation of dengue viruses to mosquito vector populations in Thailand
International audienceDespite their epidemiological importance, the evolutionary forces that shape the spatial structure of dengue virus genetic diversity are not fully understood. Fine-scale genetic structure of mosquito vector populations and evidence for genotype 9 genotype interactions between dengue viruses and their mosquito vectors are consistent with the hypothesis that the geographical distribution of dengue virus genetic diversity may reflect viral adaptation to local mosquito populations. To test this hypothesis, we measured vector competence in all sympatric and allo-patric combinations of 14 low-passage dengue virus isolates and two wild-type populations of Aedes aegypti mosquitoes sampled in Bangkok and Kamphaeng Phet, two sites located about 300 km apart in Thailand. Despite significant genotype 9 genotype interactions, we found no evidence for superior vector competence in sympatric versus allopatric vector–virus combinations. Viral phylogenetic analysis revealed no geographical clustering of the 14 isolates, suggesting that high levels of viral migration (gene flow) in Thailand may counteract spatially heterogeneous natural selection. We conclude that it is unlikely that vector mediated selection is a major driver of dengue virus adaptive evolution at the regional scale that we examined. Dengue virus local adaptation to mosquito vector populations could happen, however, in places or times that we did not test, or at a different geographical scale
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