Requirement Engineering Activities in Smart Environments for Large Facilities

Developing a large, but smart environment is a complex task that requires the collaboration of experts of different disciplines. How to successfully attain such collaboration is not a trivial matter. The paper illustrates the problem with a case study where the manager of the facility intends to influence pedestrians so that they choose a task that requires certain effort, e.g. using staircases, instead of the current one that requires less effort, e.g. using the elevator. Defining requirements for such scenarios requires a strong multidisciplinary collaboration which is not currently well supported. This paper contributes with an approach to provide non-technician experts with tools so that they can provide feedback on the requirements and verify them in a systematic way

Genetic diversity and differentiation in narrow versus widespread taxa of Helianthemum (Cistaceae) in a hotspot: The role of geographic range, habitat, and reproductive traits

We thank the authorities of the Sierra Nevada National Park and the Junta de Andalucía (Andalusian Regional Government) for granting permission to collect samples even of species under protection. We also thank the Centro de Investigación Tecnología e Innovación de la Universidad de Sevilla (“Celestino Mutis” CITIUS-2 center) for facilities to use the Coulter Multisizer 3. This research was supported by grants from the Ministerio de Agricultura, Alimentación y Medio Ambiente (National Parks Authority ref. 296/2011) and by the Ministerio de Economía y Competitividad (CGL2014-52459-P and partially CGL2013-45037-P and CGL2017-82465-P). S. MartínHernanz was supported by a fellowship from the Spanish Secretaría de Estado de Investigación, Desarrollo e Innovación (FPI 2015, BES-2015-073314). All samples in this study were collected under permission by the Sierra Nevada National Park and the Andalusian Regional GovernmentUnraveling the relationships between ecological, functional traits and genetic diversity of narrow endemic plants provide opportunities for understanding how evolutionary processes operate over local spatial scales and ultimately how diversity is created and maintained. To explore these aspects in Sierra Nevada, the core of the Mediterranean Betic‐Rifean hotspot, we have analyzed nuclear DNA microsatellite diversity and a set of biological and environmental factors (physicochemical soil parameters, floral traits, and community composition) in two strictly endemic taxa from dolomite outcrops of Sierra Nevada (Helianthemum pannosum and H. apenninum subsp. estevei ) and two congeneric widespread taxa (H. cinereum subsp. rotundifolium and H. apenninum subsp. apenninum ) that further belong to two different lineages (subgenera) of Helianthemum . We obtained rather unexpected results contrasting with the theory: (a) The narrow endemic taxa showed higher values of genetic diversity as well as higher average values of pollen production per flower and pollen‐to‐ovule ratio than their widespread relatives; and (b) the two taxa of subg. Helianthemum , with larger corollas, approach herkogamy and higher pollen production than the two taxa of subg. Plectolobum , displayed lower genetic diversity and higher values of inbreeding. Altogether, these results disclose how genetic diversity may be affected simultaneously by a large number of intrinsic and extrinsic factors, especially in Pleistocene glacial refugia in mountains where the spatial context harbors a great ecological heterogeneity. On the other hand, differences in mating system and the significant effect of the substrate profile, both being highly diverse in the genus Helianthemum , in the genetic variability illustrate about the importance of these two factors in the diversification and species differentiation of this paradigmatic genus in the Mediterranean and open the field to formulate and test new hypotheses of local adaptation, trait evolution, and habitat diversification.Secretaría de Estado de Investigación, Desarrollo e Innovación, Grant/Award Number: BES-2015-073314; Ministerio de Economía y Competitividad, Grant/Award Number: CGL2013-45037-P, CGL2014- 52459-P and CGL2017-82465-P; Ministerio de Agricultura, Alimentación y Medio Ambiente, Grant/Award Number: 296/201

JADOPPT: java based AutoDock preparing and processing tool

[EN]Motivation: AutoDock is a very popular software package for docking and virtual screening. However, currently it is hard work to visualize more than one result from the virtual screening at a time. To overcome this limitation we have designed JADOPPT, a tool for automatically preparing and processing multiple ligand-protein docked poses obtained from AutoDock. It allows the simultaneous visual assessment and comparison of multiple poses through clustering methods. Moreover, it permits the representation of reference ligands with known binding modes, binding site residues, highly scoring regions for the ligand, and the calculated binding energy of the best ranked results. Availability and Implementation: JADOPPT, supplementary material (Case Studies 1 and 2) and video tutorials are available at http://visualanalytics.land/cgarcia/JADOPPT.html Contacts: [email protected] or [email protected] Supplementary information: Supplementary data are available at Bioinformatics online

Procjena genotoksičnosti insekticida Lannate-90® i njegovih biljnih i životinjskih metabolita u kulturi ljudskih limfocita

This study evaluated direct and metabolic genotoxic effects caused by Lannate-90®, a methomyl-based formulation (90 % active ingredient), in human lymphocyte cultures using sister chromatid exchange assay (SCE). Two processes were used for the plant promutagens evaluation: in vivo activation, applying the insecticide systemically in plants for 4 h and subsequently adding plant metabolites containing extracts to lymphocyte cultures; and in vitro activation, where the insecticide was incubated with Vicia faba S10 mix plus human lymphocyte culture. Direct treatment with the insecticide significantly increased SCE frequency in human lymphocytes (250-750 mg L-1), with cellular death observed at 1000 mg L-1 concentration. Using the extracts of Vicia faba treated with Lannate-90® to treat human lymphocytes, a dose-response relationship was observed. In lymphocyte cultures treated directly with the insecticide for 2 h, a negative response was obtained. When S10 mix was added, SCE frequency did not change significantly. Meanwhile, a mixture of S9 mammalian metabolic mix and Lannate-90® increased the SCE frequency, with an observed concentration-dependent response. Although Lannate-90® induced cellular death at the highest concentrations, it did not cause a delay in cell proliferation in any of the treatments, confirming its genotoxic action. This study is one of the first to evaluate and compare the direct effect of Lannate-90® in two bioassays, animal and vegetal, and the effect of plant and animal metabolism on its genotoxic potential.Korištenjem testa izmjena sestrinskih kromatida (eng. Sister Chromatide Exchange Assay – SCE) u kulturama ljudskih limfocita ispitivani su izravni i metabolički genotoksični učinci insekticida Lannate-90®, formulacije koja se temelji na metomilu (90 % aktivni sastojak). Za procjenu biljnih promutagena provedena su dva postupka: in vivo aktivacija, kod koje se insekticid četiri sata sustavno primjenjivao na biljci, a potom su kulturama limfocita dodani biljni metaboliti s ekstraktom, i aktivacija in vitro, kod koje je insekticid inkubiran mješavinom S10 biljke Vicia faba i kulturom ljudskih limfocita. Izravno tretiranje insekticidom značajno je povećalo učestalost SCE-a u ljudskim limfocitima (250-750 mg L-1), a stanična smrt uočena je pri koncentraciji od 1000 mg L-1. Nakon tretiranja ljudskih limfocita ekstraktima biljke Vicia faba koji su tretirani insekticidom Lannate-90®, primijećen je odnos između doze i učinka. Kod kultura limfocita koje su dva sata bile izravno tretirane insekticidom primijećen je negativan odgovor. Kada je dodana S10 mješavina za metaboličku aktivaciju, učestalost SCE-a nije se značajnije promijenila. Naspram tomu, metabolička mješavina S9 za kultivirane stanice sisavaca i Lannate-90® povećali su učestalost SCE-a, uz zamijećen koncentracijski ovisan odgovor. Premda je Lannate-90® inducirao staničnu smrt pri najvišim koncentracijama, nije uzrokovao zastoj stanične proliferacije ni u jednom postupku, čime se potvrđuje njegovo genotoksično djelovanje. Ovo je ispitivanje među prvima kojim se procjenjivao i uspoređivao izravan učinak insekticida Lannate-90® u dvama biološkim testovima, životinjskom i biljnom, te učinak biljnog i životinjskog metabolizma na njegov genotoksični potencijal

Thermal behaviour of medium-voltage underground cables under high-load operating conditions

The dynamic management of electric power distribution lines has become a topic of great interest at present. Knowledge of the ampacity of cables is fundamental to carrying out dynamic management. In this study, the ampacity of buried cables in different soil resistivities and depths was calculated. A small-scale model was built in the laboratory to simulate the operating conditions of a buried cable. With the experimental results, a numerical model based on the finite element method was validated to evaluate the ampacities calculated by two standards. A comparison was made between the ampacities calculated from the IEC 60287-1 and UNE 211435 standards and those obtained from the simulated model. In addition, a comparison was made regarding the steady-state temperatures obtained at each calculated ampacity. The results obtained from the simulated model design show that the ampacity calculation method of the IEC 60287-1 standard where drying-out of the soil occurs is the most accurate, and has the least risk of exceeding the maximum permissible cable temperature.This work was financed by the EU Regional Development Fund (FEDER) and the Spanish Government under ENE-2013-42720-R, RETOS-COLABORACION RTC-2015-3795-3 and SODERCAN/FEDER Proyectos Puente 2017 and by the University of Cantabria Industrial Doctorate 19.DI12.649. The authors also acknowledge support received from Viesgo

Dynamic rating management of overhead transmission lines operating under multiple weather conditions

Integration of a large number of renewable systems produces line congestions, resulting in a problem for distribution companies, since the lines are not capable of transporting all the energy that is generated. Both environmental and economic constraints do not allow the building new lines to manage the energy from renewable sources, so the efforts have to focus on the existing facilities. Dynamic Rating Management (DRM) of power lines is one of the best options to achieve an increase in the capacity of the lines. The practical application of DRM, based on standards IEEE (Std.738, 2012) and CIGRE TB601 (Technical Brochure 601, 2014) , allows to find several deficiencies related to errors in estimations. These errors encourage the design of a procedure to obtain high accuracy ampacity values. In the case of this paper, two methodologies have been tested to reduce estimation errors. Both methodologies use the variation of the weather inputs. It is demonstrated that a reduction of the conductor temperature calculation error has been achieved and, consequently, a reduction of ampacity error.This research was funded by the Spanish Government AND FEDER funds under the R+D initiative RETOS COLABORACIÓN 2015” with reference RETOS COLABORACIÓN RTC-2015-3795-3 and Spanish R+D initiative with reference ENE2013-42720-R

Políticas de almacenamiento y reutilización de datos de investigación en revistas de cinco áreas científicas.

This study analyze the preservation and reuse policies in row research data from different journals selected from every thematic area from the Journal Citation Reports (Web of Science-JCR)

Desentrañando la historia y evolución en el principal «hotspot» de biodiversidad vegetal ibérica. Una aproximación multiescalar en el Parque Nacional de Sierra Nevada

Dadas las amenazas actuales sobre la biodiversidad del planeta, es crítico desentrañar los mecanismos evolutivos responsables de la formación de los puntos calientes de biodiversidad («hotspots»). Sierra Nevada es un «hotspot» en la cuenca mediterránea, donde una compleja historia geológica y climática ha creado diversos hábitats a los que las plantas se han adaptado, dando lugar a distintas comunidades locales y una flora regional peculiar. En este contexto, las relaciones evolutivas entre las especies son un factor clave para entender la composición final de estas comunidades. En este proyecto se ha desarrollado una aproximación filogenética al estudio de comunidades y la flora leñosa de Sierra Nevada dentro del «hotspot» de rango superior en el que se incluye (el arco Bético-Rifeño, entre el sur de España y el norte de África). Se usan datos florísticos de todo el «hotspot» Bético-Rifeño, de las sierras Béticas y de comunidades leñosas de Sierra Nevada en diferentes altitudes y sustratos, así como megafilogenias regionales de todo el «hotspot» o específicamente elaboradas para Sierra Nevada. En particular, se proporciona información para contestar las siguientes cuestiones: (i) ¿Cómo se distribuye la diversidad filogenética alfa y beta en la región Bético-Rifeña y cuál es la posición de Sierra Nevada en este contexto? (ii) ¿Cómo es la estructura filogenética y funcional de las comunidades de plantas leñosas y cómo varia dicha estructura en los gradientes altitudinales bajo diferentes sustratos? (iii) ¿Cuáles son los correlatos biológicos de estos procesos de adaptación y diferenciación? Además, para comprender mejor los mecanismos del cambio adaptativo que originan diversidad, se estudian (iv) las relaciones filogenéticas y genéticas de un género muy diverso en Sierra Nevada: Helianthemum (Cistaceae). De esta forma se ensamblan los mecanismos macro y microevolutivos que han determinado la alta biodiversidad vegetal de Sierra Nevada dentro de la región donde se encuadra.Given current threats of global change on biodiversity, it is of outstanding importance to unravel the mechanisms that allowed the evolutionary construction of biodiversity hotspots. Sierra Nevada is a hotspot within the Mediterranean basin, where a complex geological and climatic history have promoted a high diversity of habitats, moulding different plant communities and assemblages. In this context, the evolutionary history of species is critical to understand the current composition of communities. In this project, we have developed a phylogenetic approach using the woody flora of Sierra Nevada, which is nested within the Baetic-Rifan hotspot. We use floristic data for the whole Baetic-Rifan hotspot, the Baetic sierras and woody plant communities at different elevations and substrates in Sierra Nevada, as well as regional megaphylogenies for the whole hotspot and phylogenies specifically developed for Sierra Nevada. We specifically address the following key questions: (i) How is alpha and beta phylogenetic diversity distributed in the Baetic-Rifan hotspot and what is the position of Sierra Nevada within this context? (ii) What is the phylogenetic and functional structure of woody plant communities, and how does community structure vary along elevation gradients and different substrates? (iii) What are the biological correlates of these adaptive and differentiation processes? Besides, in order to get a better understanding of the mechanisms driving adaptive change and originating diversity and endemism, we study (iv) the phylogenetic and genetic relationships of a key genus of high diversity in Sierra Nevada, Helianthemum (Cistaceae). Thus, we jointly address the macro- and microevolutionary mechanisms determining the high plant biodiversity of Sierra Nevada and surrounding regions.Ministerio de Agricultura, Alimentación y Medio Ambiente de España (MAGRAMA)Ministerio para la Transición Ecológica y el Reto Demográfico de España (MITECO)Ministerio de Medio Ambiente y Medio Rural de España 296/201

Aneurisma de la orejuela izquierda secundaria a insuficiencia mitral excéntrica severa

Left atrial appendage aneurysm is an infrequent condition, which can be congenital or acquired, the latter being mainly associated with rheumatic mitral heart disease and other conditions that increase left atrial pressure. In this case, the echocardiographic study is the ideal method to reach the diagnosis and offer adequate treatment. We report the case of a 78 year old patient with a diagnosis of inactive rheumatic heart disease in whom a left atrial appendage aneurysm secondary to a severe eccentric jet of mitral insufficiency was found, in addition to a patent foramen ovale.El aneurisma de la orejuela izquierda es una condición infrecuente, la cual puede ser congénita o adquirida, siendo la segunda asociada principalmente a cardiopatía mitral reumática y otras condiciones que incrementen la presión auricular izquierda. En este caso el estudio ecocardiográfico es el método ideal para llegar al diagnóstico y ofrecer un tratamiento adecuado. Reportamos el caso de una paciente de 78 años con diagnóstico de cardiopatía reumática inactiva en la que se evidenció un aneurisma de orejuela izquierda secundaria a un jet excéntrico severo de insuficiencia mitral, además de foramen oval permeable