In-season internal and external training load quantification of an elite European soccer team

Elite soccer teams that participate in European competitions need to have players in the best physical and psychological status possible to play matches. As a consequence of congestive schedule, controlling the training load (TL) and thus the level of effort and fatigue of players to reach higher performances during the matches is therefore critical. Therefore, the aim of the current study was to provide the first report of seasonal internal and external training load that included Hooper Index (HI) scores in elite soccer players during an in-season period. Nineteen elite soccer players were sampled, using global position system to collect total distance, high-speed distance (HSD) and average speed (AvS). It was also collected session rating of perceived exertion (s-RPE) and HI scores during the daily training sessions throughout the 2015-2016 in-season period. Data were analysed across ten mesocycles (M: 1 to 10) and collected according to the number of days prior to a one-match week. Total daily distance covered was higher at the start (M1 and M3) compared to the final mesocycle (M10) of the season. M1 (5589m) reached a greater distance than M5 (4473m) (ES = 9.33 [12.70, 5.95]) and M10 (4545m) (ES = 9.84 [13.39, 6.29]). M3 (5691m) reached a greater distance than M5 (ES = 9.07 [12.36, 5.78]), M7 (ES = 6.13 [8.48, 3.79]) and M10 (ES = 9.37 [12.76, 5.98]). High-speed running distance was greater in M1 (227m), than M5 (92m) (ES = 27.95 [37.68, 18.22]) and M10 (138m) (ES = 8.46 [11.55, 5.37]). Interestingly, the s-RPE response was higher in M1 (331au) in comparison to the last mesocycle (M10, 239au). HI showed minor variations across mesocycles and in days prior to the match. Every day prior to a match, all internal and external TL variables expressed significant lower values to other days prior to a match (p<0.01). In general, there were no differences between player positions. Conclusions: Our results reveal that despite the existence of some significant differences between mesocycles, there were minor changes across the in-season period for the internal and external TL variables used. Furthermore, it was observed that MD-1 presented a reduction of external TL (regardless of mesocycle) while internal TL variables did not have the same record during in-season match-day-minus.: The authors state that there were no salaries’ fund from a tobacco company. Also, the authors are not aware of any competing interests. This project was supported by the National Funds through FCT—Portuguese Foundation for Science and Technology (UID/DTP/04045/2013)—and the European Fund for Regional Development (FEDER) allocated by European Union through the COMPETE 2020 Programme (POCI-01-0145- FEDER-006969)—competitiveness and internationalization (POCI). All funding received for this work from any of the following organizations: National Institutes of Health (NIH); Welcome Trust; Howard Hughes Medical Institute (HHMI). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.info:eu-repo/semantics/publishedVersio

In-season training load quantification of one-, two- and three-game week schedules in a top European professional soccer team

Top European soccer teams that play in UEFA competitions often participate in one, two- or three-games per week. Therefore, it is necessary to ensure optimal match-day performance and full recovery. The aim of this study was to quantify internal and external training load (TL) within five microcycles: M1 and M2 - one-game weeks; M3 and M4 - two-game weeks; M5 - three-game week). Thirteen elite soccer players participated in this study. A global positioning system (GPS) was used to measure the total distance covered and distances of different exercise training zones (1-5), the session ratings of perceived exertion (s-RPE) scores and the amount of creatine kinase (CK) created during daily training sessions for the 2015-2016 in-season period. The data were analysed with respect to the number of days prior to a given match. The main results indicate that there was a significant difference in training intensity for zone 1 between M2 and M4 (4010.2 ± 103.5 and 4507.6 ± 133.0 m, respectively); a significant difference in training intensity for zone 3 between M1 and M5 (686.1 ± 42.8 and 801.2 ± 61.2 m, respectively); a significant difference in the duration of the training sessions and matches between M2 and M5 (69.2 ± 2.1 and 79.6 ± 2.3) and M3 and M5 (69.7 ± 1.0 and 79.6 ± 2.3); and finally, there was a significant difference in CK between M3 and M2 (325.5 ± 155.0 and 194.4 ± 48.9). Moreover, there was a significant decrease in TL in the last day prior to a match, for all microcycles and all variables. There was no significant difference with respect to s-RPE. This study provides the first report of daily external and internal TLs and weekly accumulated load (training sessions and match demands) during one, two, and three-game week schedules in a group of elite soccer players. Expected significant differences are found in daily and accumulated loads for within- and between-game schedules. A similar pattern is exhibited for one- and two-game week microcycles regarding the day before the match, which exhibits a decrease in all variables. Despite the different number of games played per week, TL remain similar between microcycles for zone 2 and 5, plus s-RPE.This project was supported by the National Funds through FCT—Portuguese Foundation for Science and Technology (UID/DTP/04045/2013) and the European Fund for Regional Development (FEDER) allocated by European Union through the COMPETE 2020 Programme (POCI-01-0145-FEDER-006969)—competitiveness and internationalization (POCI). The authors disclose funding received for this work from any of the following organizations: National Institutes of Health (NIH); Welcome Trust; Howard Hughes Medical Institute (HHMI); and other(s).info:eu-repo/semantics/publishedVersio

Doped carrier formulation of the t-J model: the projection constraint and the effective Kondo-Heisenberg lattice representation

We show that the recently proposed doped carrier Hamiltonian formulation of the t-J model should be complemented with the constraint that projects out the unphysical states. With this new important ingredient, the previously used and seemingly different spin-fermion representations of the t-J model are shown to be gauge related to each other. This new constraint can be treated in a controlled way close to half-filling suggesting that the doped carrier representation provides an appropriate theoretical framework to address the t-J model in this region. This constraint also suggests that the t-J model can be mapped onto a Kondo-Heisenberg lattice model. Such a mapping highlights important physical similarities between the quasi two-dimensional heavy fermions and the high-T$_c$ superconductors. Finally we discuss the physical implications of our model representation relating in particular the small versus large Fermi surface crossover to the closure of the lattice spin gap.Comment: corrected and enlarged versio

Leaf morpho-anatomical characterization of Eremanthus erythropappus (DC.) MacLeisch, Asteraceae

Eremanthus erythropappus (DC.) MacLeisch (Asteraceae), commonly known as 'candeia-da-serra', is a plant used in folk medicine as wound healing and antimicrobial. The aim of this study was to compare the number of glandular trichomes between the young and the mature leaves, as well as to perform the morpho-anatomical characterization of E. erythropappus leaves. Transverse and paradermal sections of the leaves were prepared according to light and scanning microtechniques for the morpho-anatomical characterization. The quantification of glandular trichomes on the adaxial surface of the epidermis was evaluated in young and mature leaves with camera lucida. The leaves are alternate or fasciculate and the blade has uniseriate epidermis coated with thin and smooth cuticle and dorsiventral mesophyll. There are predominantly anomocytic stomata on both surfaces, as well as glandular trichomes located in epidermal depressions. Various non-glandular trichomes are encountered on the abaxial surface. The palisade parenchyma consists of a single layer of cells and the parenchyma which is faced to the abaxial surface comprehends three to five layers of cells in compact arrangement. The young and mature leaves showed, respectively, 21.78±5.83 e 17.80±6.69 glandular trichomes on the adaxial side. The morpho-anatomical analysis of E. erythropappus leaves has proved to be a practical and rapid method for the identification and quality control of the vegetal species used for medical purposes.Eremanthus erythropappus (DC.) MacLeisch (Asteraceae), popularmente conhecida como "candeia-da-serra", é uma espécie utilizada na medicina tradicional como cicatrizante e antimicrobiano. O objetivo do presente trabalho foi comparar o número de tricomas glandulares nas folhas jovens e adultas de E. erythropappus, assim como realizar a caracterização morfoanatômica destas folhas. Secções transversais e paradérmicas das folhas foram submetidas às microtécnicas fotônicas e à microscopia eletrônica de varredura. A quantificação dos tricomas glandulares foi realizada em folhas jovens e adultas com auxílio de câmara clara. As folhas são alternas ou fasciculadas e a lâmina foliar possui epiderme uniestratificada, revestida por cutícula delgada e lisa e mesofilo dorsiventral. Em ambas as faces da epiderme, ocorrem estômatos predominantemente anomocíticos e tricomas glandulares inseridos em depressões. Na face abaxial observam-se numerosos tricomas tectores. O parênquima paliçádico é uniestratificado e o parênquima voltado para a face abaxial é formado por três a cinco camadas de células com disposição compacta. As folhas jovens e adultas apresentam respectivamente 21,78±5,83 e 17,80±6,69 tricomas glandulares na face adaxial. A análise morfoanatômica das folhas de E. erythropappus mostra-se um método rápido e prático para a identificação e controle de qualidade de espécies vegetais utilizadas na terapêutica

Impact is a Gcn2 inhibitor that limits lifespan in caenorhabditis elegans

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)The General Control Nonderepressible 2 (GCN2) kinase is a conserved member of the integrated stress response (ISR) pathway that represses protein translation and helps cells to adapt to conditions of nutrient shortage. As such, GCN2 is required for longevity and stress resistance induced by dietary restriction (DR). IMPACT is an ancient protein that inhibits GCN2. Results: Here, we tested whether IMPACT down-regulation mimics the effects of DR in C. elegans. Knockdown of the C. elegans IMPACT homolog impt-1 activated the ISR pathway and increased lifespan and stress resistance of worms in a gcn-2-dependent manner. Impt-1 knockdown exacerbated DR-induced longevity and required several DR-activated transcription factors to extend lifespan, among them SKN-1 and DAF-16, which were induced during larval development and adulthood, respectively, in response to impt-1 RNAi. Conclusions: IMPACT inhibits the ISR pathway, thus limiting the activation of stress response factors that are beneficial during aging and required under DR.The General Control Nonderepressible 2 (GCN2) kinase is a conserved member of the integrated stress response (ISR) pathway that represses protein translation and helps cells to adapt to conditions of nutrient shortage. As such, GCN2 is required for longev141115CNQP - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)444424/2014-8474397/2011-42010/52557-02015/01316-72015/04264-82012/24490-42009/52047-52014/17145-42012/04064-02014/25068-02014/25270-32014/10814-8We thank Dr. T. Keith Blackwell and Dr. David Ron for providing the Patf-

Investigation of analgesic and anti-inflammatory activities of Echinodorus grandiflorus rhizomes methanol extract

The present work investigated the analgesic and anti-inflammatory activities of the methanol extract of the rhizomes of Echinodorus grandiflorus. For this purpose, acetic acid writhing, paw licking induced by formalin, carrageenan-induced rat paw edema and pleurisy tests were performed. The doses of 100, 200 and 400 mg/kg inhibited the contortions 34.30%, 35.84% and 36.07%, respectively. The lick of the paw 1st phase was reduced at the doses of 200 and 400 mg/kg, while the 2nd phase had reduction at 400 mg/kg. All doses inhibited the paw edema, while the volume of the exsudate was reduced 24.61% in the dose of 400 mg/kg. The doses of 200 and 400 mg/kg decreased the leukocytes migration. The results indicate that the methanol extract of E. grandiflorus can constitute target potential for use in therapies of the pain and inflammation.O presente trabalho investigou as atividades analgésica e antiinflamatória do extrato metanólico de rizomas de Echinodorus grandiflorus. Para isto, foram realizados os testes de contorções abdominais induzidas por ácido acético; tempo da lambida da pata induzida por formalina; edema de pata e pleurisia induzidas por carragenina. As doses de 100, 200 e 400 mg/kg inibiram as contorções em 34,30%, 35,84% e 36,07%, respectivamente. O tempo de lambida da primeira fase foi reduzido nas doses de 200 e 400 mg/kg, enquanto a segunda fase teve redução em 400 mg/kg. As doses testadas diminuíram o edema, enquanto o volume do exsudato foi reduzido em 24,61% na dose de 400 mg/kg. As doses de 200 e 400 mg/kg diminuíram a migração leucocitária. Os resultados indicam que o extrato de E. grandiflorus testado pode constituir alvo potencial para uso em terapias da dor e da inflamação

Shipping emissions in the Iberian Peninsula and the impacts on air quality

Marine traffic has been identified as a relevant source of pollutants, which cause known negative effects on air quality. The Iberian Peninsula is a central point in the connection of shipping traffic between the Americas, Africa, and the rest of Europe. To estimate the effects of shipping emissions inland and around the Iberian Peninsula, the EMEP/MSC-W model was run considering and not considering shipping emissions (obtained with STEAM3). Total estimated emissions of CO, CO2, SOx, NOx, and particulate matter (subdivided into elementary carbon - EC, organic carbon - OC, sulfate, and ash) for the study domain in 2015 were respectively 49, 30000, 360, 710, 4.5, 11, 32, and 3.3 kt yr(-1). Shipping emissions increased SO2 and NO2 concentrations, especially near port areas, and also increased the O-3, sulfate, and particulate matter (PM2.5 and PM10) concentrations over the entire Iberian Peninsula coastline (especially in the south coastal region). Shipping emissions were responsible for exceedances of WHO air quality guidelines for PM2.5 in areas far from the coastline, which confirms that shipping emissions can contribute negatively to air quality, both in coastal and inland areas

Estimating the health and economic burden of shipping related air pollution in the Iberian Peninsula

Air pollution is the leading cause of the global burden of disease from the environment, entailing substantial economic consequences. International shipping is a significant source of NOx, SO2, CO and PM, which can cause known negative health impacts. Thus, this study aimed to estimate the health impacts and the associated external costs of ship-related air pollution in the Iberian Peninsula for 2015. Moreover, the impact of CAP2020 regulations on 2015 emissions was studied. Log-linear functions based on WHO-HRAPIE relative risks for PM2.5 and NO2 all-cause mortality and morbidity health end-points, and integrated exposure-response functions for PM2.5 cause-specific mortality, were used to calculate the excess burden of disease. The number of deaths and years of life lost (YLL) due to NO2 ship-related emissions was similar to those of PM2.5 ship-related emissions. Estimated all-cause premature deaths attributable to PM2.5 ship-related emissions represented an average increase of 7.7% for the Iberian Peninsula when compared to the scenario without shipping contribution. Costs of around 9 100 million euro yr-1 (for value of statistical life approach - VSL) and 1 825 million euro yr(-1) (for value of life year approach - VOLY) were estimated for PM and NO2 all-cause burden of disease. For PM2.5 cause-specific mortality, a cost of around 3 475 million euro yr(-1) (for VSL approach) and 851 million euro yr(-1) (for VOLY approach) were estimated. Costs due to PM and NO2 all-cause burden represented around 0.72% and 0.15% of the Iberian Peninsula gross domestic product in 2015, respectively for VSL and VOLY approaches. For PM2.5 cause-specific mortality, costs represented around 0.28% and 0.06%, respectively, for VSL and VOLY approaches. If CAP2020 regulations had been applied in 2015, around 50% and 30% respectively of PM2.5 and NO2 ship-related mortality would been avoided. These results show that air pollution from ships has a considerable impact on health and associated costs affecting the Iberian Peninsula.This work was financially supported by: project UIDB/00511/2020 of the Laboratory for Process Engineering, Environment, Biotechnology and Energy - LEPABE-funded by national funds through the FCT/MCTES (PIDDAC) and project EMISSHIP PTDC/CTAAMB/32201/2017, funded by FEDER funds through COMPETE2020 - Programa Oper-acional Competitividade e Internacionalizacao (POCI) and by national funds (PIDDAC) through FCT/MCTES. Rafael A.O. Nunes thanks the individual research grant SFRH/BD/146159/2019, funded by the Por-tuguese Foundation for Science and Technology (FCT) . Sofia I.V. Sousathanks the Portuguese Foundation for Science and Technology (FCT) for the financial support of her work contract through the Scientific Employment Stimulus-Individual Call CEECIND/02477/2017. Dr Jalkanen would like to acknowledge the financial support from the European Union's Horizon2020 research and innovation programme under grant agreement #874990 (EMERGE project) . This work reflects only the authors' view and INEA is not responsible for any use that may be made of the information it contains