3,253 research outputs found

    Susceptibility of Chironomus plumosus larvae (Diptera: Chironomidae) to entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae): potential for control

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    Chironomidae, or non-biting midges, are found worldwide in a wide variety of aquatic habitats. During periods of mass adult eclosion they can become a nuisance and health hazard. Current control methods target the aquatic larval stage and include the use of insect growth regulators or insecticides, which may be prohibited in certain environments or affect non-target organisms. The aim of this study was to investigate whether entomopathogenic nematodes (EPNs) of the families Steinernematidae and Heterorhabditidae, currently employed for control of terrestrial crop pests, could be used as a viable biocontrol for the aquatic larval stages of the Chrionomidae, offering an alternative to current chemical methods. We demonstrate that Steinernema feltiae (Filipjev, 1934), Steinernema carpocapsae (Weiser, 1955), Steinernema kraussei (Steiner, 1923) and Heterorhabditis bacteriophora (Poinar, 1975) are able to survive in water up to 96 h and are able to parasitize and kill Chironomus plumosus (Linnaeus, 1758) larvae, with mortality observed after just 24 h exposure and with < 20% survival after 4 days. We also show that following application to the water column, EPNs sink to the bottom of the lentic water body and can remain alive for more than 96 h. Taken together, we believe that several EPN species could be developed as a valid form of biocontrol for Chironomidae

    Metabolomic profiles are gender, disease and time specific in the interleukin-10 gene-deficient mouse model of inflammatory bowel disease.

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    Metabolomic profiling can be used to study disease-induced changes in inflammatory bowel diseases (IBD). The aim of this study was to investigate the difference in the metabolomic profile of males and females as they developed IBD. Using the IL-10 gene-deficient mouse model of IBD and wild-type mice, urine at age 4, 6, 8, 12, 16, and 20 weeks was collected and analyzed by nuclear magnetic resonance (NMR) spectroscopy. Multivariate data analysis was employed to assess differences in metabolomic profiles that occurred as a consequence of IBD development and severity (at week 20). These changes were contrasted to those that occurred as a consequence of gender. Our results demonstrate that both IL-10 gene-deficient and wild-type mice exhibit gender-related changes in urinary metabolomic profile over time. Some male-female separating metabolites are common to both IL-10 gene-deficient and control wild-type mice and, therefore, appear to be related predominantly to gender maturation. In addition, we were able to identify gender-separating metabolites that are unique for IL-10 gene-deficient and wild-type mice and, therefore, may be indicative of a gender-specific involvement in the development and severity of the intestinal inflammation. The comparison of the gender-separating metabolomic profile from IL-10 gene-deficient mice and wild-type mice during the development of IBD allowed us to identify changes in profile patterns that appear to be imperative in the development of intestinal inflammation, but yet central to gender-related differences in IBD development. The knowledge of metabolomic profile differences by gender and by disease severity has potential clinical implications in the design of both biomarkers of disease as well as the development of optimal therapies

    UNUSUAL BREEDING BY SEABIRDS AT MARION ISLAND DURING 1997/98

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    In 1997/98, breeding at subantarctic Marion Island was exceptionally good for five species of seabirds capable of foraging over wide areas and for a tern. The number of king penguin Aptenodytes patagonicus chicks surviving to the start of spring in 1997 was considerably more than previously recorded. Greater numbers of wandering Diomedea exulans and grey-headed Thalassarche chrysostoma albatrosses, northern giant petrels Macronectes halli and Kerguelen terns Sterna virgata bred than previously recorded, and more southern giant petrels M. giganteus did so than in any other year since 1994. For southern giant petrels, reproductive success was higher than in any other year, as was survival of chicks of northern giant petrels. Conversely, for two seabirds that feed close to the island, gentoo penguin Pygoscelis papua and Crozet shag Phalacrocorax [atriceps] melanogenis, 1997/98 was a particularly poor breeding season. Gentoo penguins initiated breeding later than usual and fledged few chicks. The number of Crozet shags that bred decreased; probably about 25% of the adult population did not breed. For two species with an intermediate foraging range that eat mainly crustaceans, macaroni Eudyptes chrysolophus and eastern rockhopper E. chrysocome filholi penguins, breeding was not noticeably different from normal except that chicks of rockhopper penguins fledged with a slightly heavier mass than in other years. However, for both these penguins, the mass of adults on arrival at colonies decreased substantially in the following (1998/99) breeding season. The unusual breeding by most of the seabirds coincided with the El Niño Southern Oscillation (ENSO) event of 1997/98. This synchrony contrasts with lagged responses to ENSO events of seabirds that breed farther south in the Southern Ocean. Continued monitoring of seabirds over well-separated sites in the Southern Ocean may elucidate how climatic perturbations operating at a global scale impact seabirds in the region.Afr. J. mar. Sci. 25: 453–46

    A model for dislocation creep in polycrystalline Ni-base superalloys at intermediate temperatures

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    A model for creep at intermediate temperatures in polycrystalline Ni-based superalloys is presented. The model is based on describing stacking fault nucleation, propagation and subsequent shear within the matrix and precipitates. The critical energy for stacking fault nucleation is obtained by minimising the energy to form a stacking fault from dislocation partials, which is promoted by local stress concentrations. The extent of stacking fault shear at a precipitate is estimated using a force balance at the interface to determine the critical shear distance The model results are validated against creep experimental data in several polycrystalline superalloys showing good agreement. Individual contributions to creep from key microstructural features, including grain size and distribution, are studied to identify which ones are more significant. Similarly, it is shown that one of the main factors controlling the creep rate is the stacking fault energy in the as it dictates the stacking fault nucleation and shear rates. Parameter analysis on alloying additions typically used in commercial superalloys demonstrates which elements have the strongest effect on creep, highlighting how the present model can be used as tool for alloy and microstructure design against dislocation creep

    Interaction of Agulhas filaments with mesoscale turbulence: a case study

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    The inter-ocean leakage of heat and salt from the South Indian Ocean to the South Atlantic has important consequences for the global thermohaline circulation and in particular for the strength of overturning of the Atlantic Ocean as a whole. This leakage between these two subtropical gyres takes place south of Africa. The main mechanisms are the intermittent shedding of Agulhas rings from the retroflection of the Agulhas Current and the advection of Agulhas filaments from the border of the Agulhas Current, both of which move northwestward into the South Atlantic. The subsequent behaviour and mixing of Agulhas rings has been much studied over the past years, that of Agulhas filaments not at all. We report here on fortuitous hydrographic observations of the behaviour of an Agulhas filament that interacted with a number of mesoscale features shortly after formation. This suggests that Agulhas filaments may be involved in many other circulation elements and not only the Benguela upwelling front, as was surmised previously, and may mix out in a very site-specific region

    Bore seal technology topical report

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    Thermophysical, compatibility, and mechanical properties of ceramic-to-metal bore seal material

    Pathogenicity and environmental tolerance of commercial and UK native entomopathogenic nematodes (Steinernema and Heterorhabditis spp.) to the larvae of mosquitoes (Aedes aegypti and Ochlerotatus detritus)

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    Many vector mosquito species have evolved resistance to chemical insecticides and the search for novel biological control strategies warrants further attention. Entomopathogenic nematodes (EPNs) (Heterorhabditis and Steinernema spp.) have been developed as biological control agents for use against agricultural pests but whether they could be used to control aquatic mosquito larvae warrants further research. We exposed Aedes aegypti and Ochlerotatus detritus larvae to commercially available (Steinernema feltiae, S. carpocapsae, S. kraussei and Heterorhabditis bacteriophora) and wild isolated (S. affine and S. glaseri) EPNs and monitored survival over 7 days. We also exposed EPNs to water with a range of salinities and pHs found in the marshland habitats of British mosquitoes. Ae. aegypti and O. detritus were killed by commercial EPNs, but wild strains were unable to kill Ae. aegypti yet did kill O. detritus. All EPNs were capable of tolerating a wide range of pHs but showed variable tolerance to different salinities. EPNs could be used as an alternative to chemical insecticides but target species and habitat may influence choice of EPN strain in control operations

    Mollusks in the Northeastern Chukchi Sea

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    Infaunal and epifaunal mollusks of the northeastern Chukchi Sea were sampled and 139 molluscan taxa were identified. The pattern of spatial distribution of molluscan species was determined by cluster analysis, which resulted in six infaunal and five epifaunal station groups. Species characterizing various faunal groups are defined. Stepwise multiple discriminant analysis was applied to correlate benthic biological associations with environmental variables. Delineation of infaunal groups was mainly due to percentage of sand and bottom salinity, while epifaunal groups were separated by percent gravel and bottom temperature. An increase in abundance and biomass of infaunal mollusks occurred adjacent to and north and northwest of an identified bottom front between the Bering Shelf and Resident Chukchi Water and Alaska Coastal Water. Epifaunal molluscan abundance and biomass were highest near the coast. Mollusks, especially smaller species and the juvenile stages of larger species, represent a food resource for bottom-feeding predators in the study area.Key words: Chukchi Sea, mollusk, benthic, infauna, epifauna, bottom front, bottom-feeding predators, cluster analysis, discriminant analysisOn a fait un &eacute;chantillonnage des mollusques de l'endofaune et de l'&eacute;pifaune du nord-est de la mer des Tchouktches et on a identifi&eacute; 139 taxons de mollusques. On a d&eacute;termin&eacute; le sch&eacute;ma de r&eacute;partition g&eacute;ographique des esp&egrave;ces de mollusques au moyen d'une analyse typologique, qui a donn&eacute; six groupes de stations dans l'endofaune et cinq dans l'&eacute;pifaune. On d&eacute;finit des esp&egrave;ces caract&eacute;ristiques des divers groupes fauniques. On a appliqu&eacute; une analyse discriminante multiple s&eacute;quentielle pour corr&eacute;ler les associations biologiques du benthos aux variables de l'environnement. La d&eacute;limitation des groupes de l'endofaune &eacute;tait due en grande partie au taux de sable et de salinit&eacute; au fond, tandis que les groupes de l'&eacute;pifaune &eacute;taient r&eacute;partis en fonction du taux de gravier et de temp&eacute;rature au fond. Une augmentation dans la quantit&eacute; et la biomasse des mollusques de l'endofaune apparaissait pr&egrave;s du nord et du nord-ouest d'un front de fond compris entre le plateau continental, les eaux non brass&eacute;es de la mer des Tchouktches et les eaux c&ocirc;ti&egrave;res de l'Alaska. C'est pr&egrave;s de la c&ocirc;te qu'on retrouvait l'abondance et la biomasse maximales des mollusques de l'&eacute;pifaune. Les mollusques, surtout ceux des petites esp&egrave;ces et ceux des grandes esp&egrave;ces qui &eacute;taient au stade juv&eacute;nile, repr&eacute;sentaient une source alimentaire pour les pr&eacute;dateurs benthiques vivant dans la zone d'&eacute;tude.Mots cl&eacute;s : mer des Tchouktches, mollusque, benthique, enfofaune, &eacute;pifaune, front au fond, pr&eacute;dateurs benthiques, analyse typologique, analyse discriminant

    The Cyborg Astrobiologist: Testing a Novelty-Detection Algorithm on Two Mobile Exploration Systems at Rivas Vaciamadrid in Spain and at the Mars Desert Research Station in Utah

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    (ABRIDGED) In previous work, two platforms have been developed for testing computer-vision algorithms for robotic planetary exploration (McGuire et al. 2004b,2005; Bartolo et al. 2007). The wearable-computer platform has been tested at geological and astrobiological field sites in Spain (Rivas Vaciamadrid and Riba de Santiuste), and the phone-camera has been tested at a geological field site in Malta. In this work, we (i) apply a Hopfield neural-network algorithm for novelty detection based upon color, (ii) integrate a field-capable digital microscope on the wearable computer platform, (iii) test this novelty detection with the digital microscope at Rivas Vaciamadrid, (iv) develop a Bluetooth communication mode for the phone-camera platform, in order to allow access to a mobile processing computer at the field sites, and (v) test the novelty detection on the Bluetooth-enabled phone-camera connected to a netbook computer at the Mars Desert Research Station in Utah. This systems engineering and field testing have together allowed us to develop a real-time computer-vision system that is capable, for example, of identifying lichens as novel within a series of images acquired in semi-arid desert environments. We acquired sequences of images of geologic outcrops in Utah and Spain consisting of various rock types and colors to test this algorithm. The algorithm robustly recognized previously-observed units by their color, while requiring only a single image or a few images to learn colors as familiar, demonstrating its fast learning capability.Comment: 28 pages, 12 figures, accepted for publication in the International Journal of Astrobiolog
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