Molecular genetic and crystal structural analysis of 1-(4-hydroxyphenyl)-ethanol dehydrogenase from 'Aromatoleum aromaticum' EbN1.

he dehydrogenation of 1-(4-hydroxyphenyl)-ethanol to 4-hydroxyacetophenone represents the second reaction step during anaerobic degradation of p-ethylphenol in the denitrifying bacterium ‘Aromatoleum aromaticum' EbN1. Previous proteogenomic studies identified two different proteins (ChnA and EbA309) as possible candidates for catalyzing this reaction [Wöhlbrand et al: J Bacteriol 2008;190:5699-5709]. Physiological-molecular characterization of newly generated unmarked in-frame deletion and complementation mutants allowed defining ChnA (renamed here as Hped) as the enzyme responsible for 1-(4-hydroxyphenyl)-ethanol oxidation. Hped [1-(4-hydroxyphenyl)-ethanol dehydrogenase] belongs to the ‘classical' family within the short-chain alcohol dehydrogenase/reductase (SDR) superfamily. Hped was overproduced in Escherichia coli, purified and crystallized. The X-ray structures of the apo- and NAD+-soaked form were resolved at 1.5 and 1.1 Å, respectively, and revealed Hped as a typical homotetrameric SDR. Modeling of the substrate 4-hydroxyacetophenone (reductive direction of Hped) into the active site revealed the structural determinants of the strict (R)-specificity of Hped (Phe187), contrasting the (S)-specificity of previously reported 1-phenylethanol dehydrogenase (Ped; Tyr93) from strain EbN1 [Höffken et al: Biochemistry 2006;45:82-93]

Ground-based near-IR observations of the secondary eclipse of CoRoT-2b

We present the results of a ground-based search for the secondary eclipse of the 3.3 Mjup transiting planet CoRoT-2b. We performed near infrared photometry using the LIRIS instrument on the 4.2 m William Herschel Telescope, in the H and K_s filters. We monitored the star around two expected secondary eclipses in two nights under very good observing conditions. For the depth of the secondary eclipse we find in H-band a 3 sigma upper limit of 0.17%, whereas we detected a tentative eclipse with a depth of 0.16+-0.09% in the K_s-band. These depths can be translated into brightness temperatures of T_H<2250 K and T_{K_s} = 1890(+260-350) K, which indicate an inefficient re-distribution of the incident stellar flux from the planet's dayside to its nightside. Our results are in agreement with the CoRoT optical measurement (Alonso et al. 09) and with Spitzer 4.5 and 8 micron results (Gillon et al. 09c).Comment: Astronomical Journal, accepte

The response of Black Rapids Glacier, Alaska, to the Denali earthquake rock avalanches

We describe the impact of three simultaneous earthquake-triggered rock avalanches on the dynamics of Black Rapids Glacier, Alaska, by using spaceborne radar imagery and numerical modeling. We determined the velocities of the glacier before and after landslide deposition in 2002 by using a combination of ERS-1/ERS-2 tandem, RADARSAT-1, and ALOS PALSAR synthetic aperture radar data. Ice velocity above the debris-covered area of the glacier increased up to 14% after the earthquake but then decreased 20% by 2005. Within the area of the debris sheets, mean glacier surface velocity increased 44% within 2 years of the landslides. At the downglacier end of the lowest landslide, where strong differential ablation produced a steep ice cliff, velocities increased by 109% over the same period. By 2007, ice velocity throughout the debris area had become more uniform, consistent with a constant ice flux resulting from drastically reduced ablation at the base of the debris. Without further analysis, we cannot prove that these changes resulted from the landslides, because Black Rapids Glacier displays large seasonal and interannual variations in velocity. However, a full Stokes numerical ice flow model of a simplified glacier geometry produced a reversal of the velocity gradient from compressional to extensional flow after 5 years, which supports our interpretation that the recent changes in the velocity field of the glacier are related to landslide-induced mass balance changes

Planetary eclipse mapping of CoRoT-2a. Evolution, differential rotation, and spot migration

The lightcurve of CoRoT-2 shows substantial rotational modulation and deformations of the planet's transit profiles caused by starspots. We consistently model the entire lightcurve, including both rotational modulation and transits, stretching over approximately 30 stellar rotations and 79 transits. The spot distribution and its evolution on the noneclipsed and eclipsed surface sections are presented and analyzed, making use of the high resolution achievable under the transit path. We measure the average surface brightness on the eclipsed section to be (5\pm1) % lower than on the noneclipsed section. Adopting a solar spot contrast, the spot coverage on the entire surface reaches up to 19 % and a maximum of almost 40 % on the eclipsed section. Features under the transit path, i.e. close to the equator, rotate with a period close to 4.55 days. Significantly higher rotation periods are found for features on the noneclipsed section indicating a differential rotation of $\Delta \Omega > 0.1$. Spotted and unspotted regions in both surface sections concentrate on preferred longitudes separated by roughly 180 deg.Comment: Paper accepted by A&A 17/02/2010. For a better resolution paper please visit my homepage: http://www.hs.uni-hamburg.de/EN/Ins/Per/Huber/index.htm

Limits to the planet candidate GJ 436c

We report on H-band, ground-based observations of a transit of the hot Neptune GJ 436b. Once combined to achieve sampling equivalent to archived observations taken with Spitzer, our measurements reach comparable precision levels. We analyze both sets of observations in a consistent way, and measure the rate of orbital inclination change to be of 0.02+/-0.04 degrees in the time span between the two observations (253.8 d, corresponding to 0.03+/-0.05 degrees/yr if extrapolated). This rate allows us to put limits on the relative inclination between the two planets by performing simulations of planetary systems, including a second planet, GJ 436c, whose presence has been recently suggested (Ribas et al. 2008). The allowed inclinations for a 5 M_E super-Earth GJ 436c in a 5.2 d orbit are within ~7 degrees of the one of GJ 436b; for larger differences the observed inclination change can be reproduced only during short sections (<50%) of the orbital evolution of the system. The measured times of three transit centers of the system do not show any departure from linear ephemeris, a result that is only reproduced in <1% of the simulated orbits. Put together, these results argue against the proposed planet candidate GJ 436c.Comment: Replaced with accepted version. Minor language corrections. 4 pages, 4 figures, to appear in A&A Letter

A cool starspot or a second transiting planet in the TrES-1 system?

We investigate the origin of a flux increase found during a transit of TrES-1, observed with the HST. This feature in the HST light curve cannot be attributed to noise and is supposedly a dark area on the stellar surface of the host star eclipsed by TrES-1 during its transit. We investigate the likeliness of two possible hypothesis for its origin: A starspot or a second transiting planet. We made use of several transit observations of TrES-1 from space with the HST and from ground with the IAC-80 telescope. On the basis of these observations we did a statistical study of flux variations in each of the observed events, to investigate if similar flux increases are present in other parts of the data set. The HST observation presents a single clear flux rise during a transit whereas the ground observations led to the detection of two such events but with low significance. In the case of having observed a starspot in the HST data, assuming a central impact between the spot and TrES-1, we would obtain a lower limit for the spot radius of 42000 km. For this radius the spot temperature would be 4690 K, 560 K lower then the stellar surface of 5250 K. For a putative second transiting planet we can set a lower limit for its radius at 0.37 R$_J$ and for periods of less than 10.5 days, we can set an upper limit at 0.72 R$_J$. Assuming a conventional interpretation, then this HST observation constitutes the detection of a starspot. Alternatively, this flux rise might also be caused by an additional transiting planet. The true nature of the origin can be revealed if a wavelength dependency of the flux rise can be shown or discarded with a higher certainty. Additionally, the presence of a second planet can also be detected by radial velocity measurements.Comment: 8 pages, 6 figures, accepted for publication in A&

The HARPS search for southern extra-solar planets XXXII. Only 4 planets in the Gl~581 system

The Gl 581 planetary system has generated wide interest, because its 4 planets include both the lowest mass planet known around a main sequence star other than the Sun and the first super-Earth planet in the habitable zone of its star. A recent paper announced the possible discovery of two additional super-Earth planets in that system, one of which would be in the middle of the habitable zone of Gl 581. The statistical significance of those two discoveries has, however, been questioned. We have obtained 121 new radial velocity measurements of Gl 581 with the HARPS spectrograph on the ESO 3.6 m telescope, and analyse those together with our previous 119 measurements of that star to examine these potential additional planets. We find that neither is likely to exist with their proposed parameters. We also obtained photometric observations with the 2.5 m Isaac Newton Telescope during a potential transit of the inner planet, Gl 581e, which had a 5% geometric transit probability. Those observations exclude transits for planet densities under 4 times the Earth density within -0.2 sigma to +2.7 sigma of the predicted transit center.Comment: Submitted to Astronomy & Astrophysic

Ground-based photometry of space-based transit detections: Photometric follow-up of the CoRoT mission

The motivation, techniques and performance of the ground-based photometric follow-up of transit detections by the CoRoT space mission are presented. Its principal raison d'\^{e}tre arises from the much higher spatial resolution of common ground-based telescopes in comparison to CoRoT's cameras. This allows the identification of many transit candidates as arising from eclipsing binaries that are contaminating CoRoT's lightcurves, even in low-amplitude transit events that cannot be detected with ground-based obervations. For the ground observations, 'on'-'off' photometry is now largely employed, in which only a short timeseries during a transit and a section outside a transit is observed and compared photometrically. CoRoT planet candidates' transits are being observed by a dedicated team with access to telescopes with sizes ranging from 0.2 to 2 m. As an example, the process that led to the rejection of contaminating eclipsing binaries near the host star of the Super-Earth planet CoRoT-7b is shown. Experiences and techniques from this work may also be useful for other transit-detection experiments, when the discovery instrument obtains data with a relatively low angular resolution.Comment: Accepted for the A&A special issue on CoRo