4,904 research outputs found
Structure and Metal Binding Properties of ZnuA, a Periplasmic Zinc Transporter from \u3cem\u3eEscherichia coli\u3c/em\u3e
ZnuA is the periplasmic Zn2+-binding protein associated with the high-affinity ATP-binding cassette ZnuABC transporter from Escherichia coli. Although several structures of ZnuA and its homologs have been determined, details regarding metal ion stoichiometry, affinity, and specificity as well as the mechanism of metal uptake and transfer remain unclear. The crystal structures of E. coli ZnuA (Eco-ZnuA) in the apo, Zn2+-bound, and Co2+-bound forms have been determined. ZnZnuA binds at least two metal ions. The first, observed previously in other structures, is coordinated tetrahedrally by Glu59, His60, His143, and His207. Replacement of Zn2+ with Co2+ results in almost identical coordination geometry at this site. The second metal binding site involves His224 and several yet to be identified residues from the His-rich loop that is unique to Zn2+ periplasmic metal binding receptors. Electron paramagnetic resonance and X-ray absorption spectroscopic data on CoZnuA provide additional insight into possible residues involved in this second site. The second site is also detected by metal analysis and circular dichroism (CD) titrations. Eco-ZnuA binds Zn2+ (estimated K d \u3c 20 nM), Co2+, Ni2+, Cu2+, Cu+, and Cd2+, but not Mn2+. Finally, conformational changes upon metal binding observed in the crystal structures together with fluorescence and CD data indicate that only Zn2+ substantially stabilizes ZnuA and might facilitate recognition of ZnuB and subsequent metal transfer
Tunable Electron Multibunch Production in Plasma Wakefield Accelerators
Synchronized, independently tunable and focused J-class laser pulses are
used to release multiple electron populations via photo-ionization inside an
electron-beam driven plasma wave. By varying the laser foci in the laboratory
frame and the position of the underdense photocathodes in the co-moving frame,
the delays between the produced bunches and their energies are adjusted. The
resulting multibunches have ultra-high quality and brightness, allowing for
hitherto impossible bunch configurations such as spatially overlapping bunch
populations with strictly separated energies, which opens up a new regime for
light sources such as free-electron-lasers
Energy Loss of a High Charge Bunched Electron Beam in Plasma
There has been much interest in the blowout regime of plasma wakefield
acceleration (PWFA), which features ultra-high fields and nonlinear plasma
motion. Using an exact analysis, we examine here a fundamental limit of
nonlinear PWFA excitation, by an infinitesimally short, relativistic electron
beam. The beam energy loss in this case is shown to be linear in charge even
for nonlinear plasma response, where a normalized, unitless charge exceeds
unity. The physical basis for this effect is discussed, as are deviations from
linear behavior observed in simulations with finite length beams.Comment: Submitted to Physical Review Letter
Radiative Losses in Plasma Accelerators
We investigate the dynamics of a relativistic electron in a strongly
nonlinear plasma wave in terms of classical mechanics by taking into account
the action of the radiative reaction force. The two limiting cases are
considered. In the first case where the energy of the accelerated electrons is
low, the electron makes many betatron oscillations during the acceleration. In
the second case where the energy of the accelerated electrons is high, the
betatron oscillation period is longer than the electron residence time in the
accelerating phase. We show that the force of radiative friction can severely
limit the rate of electron acceleration in a plasma accelerator.Comment: 17 pages, 5 figure
Oxidation of Methane by a Biological Dicopper Centre
Vast world reserves of methane gas are underutilized as a feedstock for the production of liquid fuels and chemicals owing to the lack of economical and sustainable strategies for the selective oxidation of methane to methanol1. Current processes to activate the strong C–H bond (104 kcal mol−1) in methane require high temperatures, are costly and inefficient, and produce waste2. In nature, methanotrophic bacteria perform this reaction under ambient conditions using metalloenzymes called methane monooxygenases (MMOs). MMOs thus provide the optimal model for an efficient, environmentally sound catalyst3. There are two types of MMO. Soluble MMO (sMMO),expressed by several strains of methanotrophs under copper-limited conditions, oxidizes methane with a well-characterized catalytic di-iron centre4. Particulate MMO (pMMO) is an integral membrane metalloenzyme produced by all methanotrophs and is composed of three subunits, pmoA, pmoB and pmoC, arranged in a trimeric α3β3γ3 complex5. Despite 20 years of research and the availability of two crystal structures, the metal composition and location of the pMMO metal active site are not known. Here we show that pMMO activity is dependent on copper, not iron, and that the copper active site is located in the soluble domains of the pmoB subunit rather than within the membrane. Recombinant soluble fragments of pmoB (spmoB) bind copper and have propylene and methane oxidation activities. Disruption of each copper centre in spmoB by mutagenesis indicates that the active site is a dicopper centre. These findings help resolve the pMMO controversy and provide a promising new approach to developing environmentally friendly C–H oxidation catalysts
Comment on ``Critical Behavior in Disordered Quantum Systems Modified by Broken Time--Reversal Symmetry''
In a recent Letter [Phys. Rev. Lett. 80, 1003 (1998)] Hussein and Pato
employed the maximum entropy principle (MEP) in order to derive interpolating
ensembles between any pair of universality classes in random matrix theory.
They apply their formalism also to the transition from random matrix to Poisson
statistics of spectra that is observed for the case of the Anderson-type
metal-insulator transition. We point out the problems with the latter
procedure.Comment: 1 page in PS, to appear in PRL Sept. 2
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