Dried brewers grains in growing rabbits: nutritional value and effects on performance

[EN] Two assays were carried out to determine i) the nutritional value of dried brewers’ grains and ii) the effects of inclusion of this ingredient in growing rabbit diets on animal performance and economic performance of the breeding unit. In the digestibility assay, a total of 28 male rabbits were distributed in 2 groups differing in the diet offered to animals: a reference diet (35.51% neutral detergent fibre and 16.50% crude protein [CP]) and a test diet (60% of reference diet and 40% of dried brewers grains). The dried brewers’ grain contained 37.9% of CP and 3371 kcal digestible energy/kg dry matter. In the performance study, 80 weaned rabbits (40 males and 40 females) were allotted at 40 d of age to 5 groups differing in the inclusion levels of dried brewers’ grains (0, 7, 14, 21 and 28%) from 40 d to 90 d of age. Inclusion of dried brewers’ grains did not affect the live weight at 90 d, the feed intake between 40 d and 90 d or the dressing percentage of rabbits (on average 223 g, 96 g/d and 51.3%, respectively). There was no effect of diet on the meat quality parameters (69.5% water holding capacity, 25.6% cooking loss, 3.4 kg/cm2 Warner-Bratzler shear force and pH 5.70) and inclusion levels above 14% reduced the feed cost (–18%; P<0.001), while inclusion above 21% improved net income (+32%; P<0.001). In conclusion, these results suggest that the use of dried brewers’ grains in diets for growing rabbits could improve the economic performance of the production system without impairing the animals’ performance.Lima, P.; Watanabe, P.; Cândido, R.; Ferreira, A.; Vieira, A.; Rodrigues, B.; Nascimento, G.... (2017). Dried brewers grains in growing rabbits: nutritional value and effects on performance. World Rabbit Science. 25(3):251-260. doi:10.4995/wrs.2017.6813.SWORD251260253Albuquerque, D. M. N., Lopes, J. B., Klein Junior, M. H., Merval, R. R., Silva, F. E. S., & Teixeira, M. P. F. (2011). Resíduo desidratado de cervejaria para suínos em terminação. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 63(2), 465-472. doi:10.1590/s0102-09352011000200026AOAC International. 2005. Official methods of analysis of Association Official Analytical Chemists International. AOAC, Arlington VA. USA.Briganó, M. V., Pacheco, G. D., Bridi, A. M., Oba, A., Fonseca, N. A. N., & Silva, C. A. da. (2008). Desempenho e características de carcaça de suínos submetidos a diferentes programas de restrição alimentar na fase dos 30 aos 118 kg. Revista Brasileira de Zootecnia, 37(8), 1398-1404. doi:10.1590/s1516-35982008000800009De Blas, J. C., Rodriguez, C. A., Bacha, F., Fernandez, R., & Abad-Guamán, R. (2015). Nutritive value of co-products derived from olivecake in rabbit feeding. World Rabbit Science, 23(4), 255. doi:10.4995/wrs.2015.4036Etchu K.A., Humbu M.E., Ndamukong K.J.N., Agbor E.B. 2012. Effect of varying levels of brewers' dried grain on the growth performance of weaner rabbits (Oryctolagus Cuniculus). Greener J. Agric. Sci., 2: 237-245.Fazano A.R.T., Zinsly C.F., Mattos W.R.S., Packer I.H. 1986. Digestibilidade e valor biológico da proteína da levedura seca (Saccharomyces spp.) e do farelo de soja para coelhos. Boletim de Indústria Animal, 46: 185-191.Lounaouci-Ouyaed G., Lakabi-ioualitene D., Berchiche M., Lebas F. 2008. Field beans and brewers grain as protein source for growing rabbits in Algeria: first results on growth and carcass quality. Nutrition and Digestive Physiology. In: 9th World Rabbit Congress, June 10-13, Verona, Italy. 723-728.Matterson L.D., Potter L.M., Stutz M.W., Singsen E.P. 1965. The metabolizable energy of feed ingredients for chickens. Storrs: University of Connecticut; Agricultural Experiment Station Research Report, 11: 11.National Research Council. 2007. Nutrient requirements of small ruminants: sheep, goats, cervids, and New World camelids. Washington, D.C.: National Academic Press, 292.Partridge G., Wyatt C. 1995. More flexibility with new generation of enzymes. World Poultry,11: 17-21.SAS 2000. SAS/STAT User's Guide (Release 6.12). SAS Inst. Inc., Cary NC, EUA.Villamide, M. J. (1996). Methods of energy evaluation of feed ingredients for rabbits and their accuracy. Animal Feed Science and Technology, 57(3), 211-223. doi:10.1016/0377-8401(95)00855-

Distribution of small proteoglycans and glycosaminoglycans in humerus-related articular cartilage of chickens

The expression of components present in the cartilaginous extracellular matrix is related to development, gender, and genotype, as well as to the biomechanical properties of each type of cartilage. In the present study, we analyzed small proteoglycans and glycosaminoglycans present in different cartilages of the chicken wing after extraction with guanidine hydrochloride or papain. Quantitative analysis of glycosaminoglycans showed a larger amount in humeral cartilage (around 200 mg/g tissue) than in articular cartilage of the radius and ulna, with 138 and 80 mg/g tissue, respectively. Non-collagenous proteins isolated were predominantly from cartilage in the proximal regions of the humerus and radius. D4 fractions obtained by ultracentrifugation were separated by DEAE-Sephacel and Octyl-Sepharose chromatography and analyzed by SDS-PAGE. Two bands of 57 and 70-90 kDa were observed for all samples treated with ß-mercaptoethanol. Immunoblotting of these proteins was positive for the small proteoglycans fibromodulin and decorin, respectively. Apparently, the 57-kDa protein is present in macromolecular complexes of 160 and 200 kDa. Chondroitin sulfate was detected in all regions. HPLC analysis of the products formed by chondroitinase AC and ABC digestion mainly revealed ß-D-glucuronic acid and N-acetyl ß-D-galactosamine residues. The 4-sulfation/6-sulfation ratio was close to 3, except for the proximal cartilage of the radius (2.5). These results suggest functional differences between the scapula-humerus, humerus-ulna, and humerus-radius joints of the chicken wing. This study contributes to the understanding of the physiology of cartilage and joints of birds under different types of mechanical stress.38139

A dune with a view: the eyes of a neotropical fossorial lizard

BackgroundLizards are excellent models to study the adaptations of the visual system to different scenarios, and surface-dwelling representatives have been relatively well studied. In contrast, very little is known about the functional anatomy of the eyes of fossorial lineages, and properties such as the light transmission by the ocular media have never been characterised in any fossorial species. Some lizards in the family Gymnophthalmidae endemic to the sand dunes of North Eastern Brazil have evolved sand-burrowing habits and nocturnal activity. Lizards in the sister group to Gymnophthalmidae, the family Teiidae, have decidedly diurnal and epigeal lifestyles, yet they are equally poorly known in terms of visual systems. We focussed on the eye anatomy, photoreceptor morphology and light transmittance properties of the ocular media and oil droplets in the gymnophthalmid Calyptommatus nicterus and the teiid Ameivula ocellifera.ResultsThe general organisation of the eyes of the fossorial nocturnal C. nicterus and the epigeal diurnal A. ocellifera is remarkably similar. The lenses are highly transmissive to light well into the ultraviolet part of the spectrum. The photoreceptors have the typical cone morphology, with narrow short outer segments and oil droplets. The main difference between the two species is that C. nicterus has only colourless oil droplets, whereas A. ocellifera has colourless as well as green-yellow and pale-orange droplets.ConclusionsOur results challenge the assumption that fossorial lizards undergo loss of visual function, a claim that is usually guided by the reduced size and external morphology of their eyes. In the case of C. nicterus, the visual system is well suited for vision in bright light and shows specialisations that improve sensitivity in dim light, suggesting that they might perform some visually-guided behaviour above the surface at the beginning or the end of their daily activity period, when light levels are relatively high in their open dunes habitat. This work highlights how studies on the functional anatomy of sensory systems can provide insights into the habits of secretive species

Aplicación de Proteómica a la búsqueda de nuevos mecanismos tumorales en cáncer de tiroides

Comunicaciones a congreso

Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured

Os efeitos do α-tocoferol e da ternatina sobre morfologia, ativação e crescimento de folículos pré-antrais caprinos cultivados in vitro, por um ou cinco dias, foram avaliados. Os fragmentos ovarianos foram imediatamente fixados (controle não-cultivado) ou cultivados in vitro, por um ou cinco dias, em Meio Essencial Mínimo (MEM) com ou sem suplementação com α-tocoferol ou ternatina nas concentrações de 5, 10 ou 15M, formando os tratamentos MEM, TOC5, TOC10, TOC 15, TER5, TER10, TER15. O percentual de folículos pré-antrais normais no controle não-cultivado foi de 73,2%, depois de cinco dias de cultivo, houve redução desse percentual em todos os tratamentos, quando comparados com o controle não-cultivado (P<0,05). O cultivo por cinco dias aumentou a ativação folicular em todos os tratamentos (P<0,05). A análise ultra-estrutural não mostrou folículos pré-antrais íntegros após cinco dias de cultivo em meio contendo antioxidantes. Concluiu-se que o α -tocoferol e a ternatina podem promover a ativação folicular, no entanto a adição desses antioxidantes nas concentrações testadas reduziu a viabilidade folicular após o cultivo in vitro. ______________________________________________________________________________________________________________ ABSTRACTThe effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without α-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15M, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P<0.05) when compared with non-cultured control. Culture of ovarian cortex for five days increased the percentages of follicular activation in all treatments (P<0.05). Ultrastructural analysis did not confirm the integrity of caprine preantral follicles cultured for five days in medium containing antioxidants. This study demonstrated that α-tocopherol and ternatin can promote follicular activation; however, addition of these antioxidants in the tested concentrations reduced the follicular viability after in vitro culture