DNA extraction from formalin-fixed Franciscana tissues

The present paper reports the extraction of DNA from formalin-fixed Pontoporia blainvillei tissues. Following the Vachot and Monerot (1996) protocol, fragmented DNA (300-700bp) was extracted from more than 95% of liver and muscle samples. DNA yield in liver samples was significantly higher than in muscle samples (4.574 ± 1.169mg DNA/mg versus 0.808 ± 0.297mg DNA/mg). Similar results were obtained from nine other species of cetaceans and five species of pinnipeds. It is of special interest to have a method that allows the utilisation of museum specimens not originally preserved for genetic studies, which may include rarely available, declining or extinct species. SPANISH: El presente trabajo reporta la extracción de ADN a partir de tejidos formolizados de Pontoporia blainvillei. Siguiendo el protocolo de Vachot y Monerot (1996) se pudo extraer ADN degradado (300-700pb) en más del 95% de las muestras de hígado y músculo analizadas. El rendimiento en ADN fue significativamente mayor en muestras de hígado que en muestras de músculo (4.574 ± 1.169mg DNA/mg tejido húmedo versus 0.808 ± 0.297mg DNA/mg tejido húmedo). Resultados similares se obtuvieron en otras nueve especies de Cetáceos y cinco de Pinnípedos. Resulta de gran interés contar con un método que permita la utilización de especímenes depositados en museos y que no hayan sido originalmente colectados para estudios genéticos, incluyendo especies de difícil obtención, en franca declinación o extintas

Triaxial Angular Momentum Projection and Configuration Mixing calculations with the Gogny force

We present the first implementation in the $(\beta,\gamma)$ plane of the generator coordinate method with full triaxial angular momentum and particle number projected wave functions using the Gogny force. Technical details about the performance of the method and the convergence of the results both in the symmetry restoration and the configuration mixing parts are discussed in detail. We apply the method to the study of $^{24}$Mg, the calculated energies of excited states as well as the transition probabilities are compared to the available experimental data showing a good overall agreement. In addition, we present the RVAMPIR approach which provides a good description of the ground and gamma bands in the absence of strong mixing.Comment: 40 pages,14 figure

Mean field study of structural changes in Pt isotopes with the Gogny interaction

The evolution of the nuclear shapes along the triaxial landscape is studied in the Pt isotopic chain using the selfconsistent Hartree-Fock-Bogoliubov approximation based on the Gogny interaction. In addition to the parametrization D1S, the new incarnations D1N and D1M of this force are also included in our analysis to asses to which extent the predictions are independent of details of the effective interaction. The considered range of neutron numbers 88<N<26 includes prolate, triaxial, oblate and spherical ground state shapes and serves for a detailed comparison of the predictions obtained with the new sets D1N and D1M against the ones provided by the standard parametrization Gogny-D1S in a region of the nuclear landscape for which experimental and theoretical fingerprints of shape transitions have been found. Structural evolution along the Pt chain is discussed in terms of the deformation dependence of single particle energies.Comment: 18 pages, 10 figures. Accepted for publication in Phys. Rev.

Role of triaxiality in the ground state shape of neutron rich Yb, Hf, W, Os, and Pt isotopes

The evolution of the ground-state shape along the triaxial landscape of several isotopes of Yb, Hf, W, Os, and Pt is analyzed using the self-consistent Hartree-Fock-Bogoliubov approximation. Two well reputed interactions (Gogny D1S and Skyrme SLy4) have been used in the study in order to asses to which extent the results are independent of the details of the effective interaction. A large number of even-even nuclei, with neutron numbers from N=110 up to N=122 has been considered, covering in this way a vast extension of the nuclear landscape where signatures of oblate-prolate shape transitions have already manifested both theoretically and experimentally.Comment: 21 pages, 8 figure

Effective shell model Hamiltonians from density functional theory: quadrupolar and pairing correlations

We describe a procedure for mapping a self-consistent mean-field theory (also known as density functional theory) into a shell model Hamiltonian that includes quadrupole-quadrupole and monopole pairing interactions in a truncated space. We test our method in the deformed N=Z sd-shell nuclei Ne-20, Mg-24 and Ar-36, starting from the Hartree-Fock plus BCS approximation of the USD shell model interaction. A similar procedure is then followed using the SLy4 Skyrme energy density functional in the particle-hole channel plus a zero-range density-dependent force in the pairing channel. Using the ground-state solution of this density functional theory at the Hartree-Fock plus BCS level, an effective shell model Hamiltonian is constructed. We use this mapped Hamiltonian to extract quadrupolar and pairing correlation energies beyond the mean field approximation. The rescaling of the mass quadrupole operator in the truncated shell model space is found to be almost independent of the coupling strength used in the pairing channel of the underlying mean-field theory.Comment: 15 pages, 5 figure

Effect of deformation on two-neutrino double beta decay matrix elements

We study the effect of deformation on the two-neutrino double beta decay for ground state to ground state transitions in all the nuclei whose half-lives have been measured. Our theoretical framework is a deformed QRPA based in Woods-Saxon or Hartree-Fock mean fields. We are able to reproduce at the same time the main characteristics of the two single beta branches, as well as the double beta matrix elements. We find a suppression of the double beta matrix element with respect to the spherical case when the parent and daughter nuclei have different deformations

Multi-component symmetry-projected approach for molecular ground state correlations

The symmetry-projected Hartree--Fock ansatz for the electronic structure problem can efficiently account for static correlation in molecules, yet it is often unable to describe dynamic correlation in a balanced manner. Here, we consider a multi-component, systematically-improvable approach, that accounts for all ground state correlations. Our approach is based on linear combinations of symmetry-projected configurations built out of a set of non-orthogonal, variationally optimized determinants. The resulting wavefunction preserves the symmetries of the original Hamiltonian even though it is written as a superposition of deformed (broken-symmetry) determinants. We show how short expansions of this kind can provide a very accurate description of the electronic structure of simple chemical systems such as the nitrogen and the water molecules, along the entire dissociation profile. In addition, we apply this multi-component symmetry-projected approach to provide an accurate interconversion profile among the peroxo and bis($\mu$-oxo) forms of [Cu$_2$O$_2$]$^{2+}$, comparable to other state-of-the-art quantum chemical methods

Seasonal and depth-driven changes in rhodolith bed structure and associated macroalgae off Arvoredo island (southeastern Brazil)

Rhodoliths are formed by coralline red algae and can form heterogeneous substrata with high biodiversity. Here we describe a rhodolith bed at the southern limit of the known distribution of this habitat in the western Atlantic. We characterized rhodolith and macroalgal assemblages at 5, 10 and 15. m depth during summer and winter. Lithothamnion crispatum was dominant amongst the six rhodolith-forming species present. Most rhodoliths were spheroidal in shape indicating high mobility due to water movement. Rhodolith density decreased with increasing depth and during winter. Turf-forming seaweeds accounted for 60% of the biomass growing on rhodoliths. Macroalgae increased abundance and richness in the summer, but was similar between 5 and 15. m depth. They were less abundant and diverse than that recorded in rhodolith beds further north in Brazil. Both, season and depth, affected the structure of the macroalgae assemblages. We conclude that Lithothamniom is the most representative genus of Brazilian rhodolith beds. Summer is responsible for increasing the diversity and richness of macroalgae, as well as increasing rhodolith density. © 2013 Elsevier B.V