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Construction of "self" and "out-there": the narratives and organisational development of Business Angels in Portugal and the United Kingdom
This is the first research conducted into Portuguese business angels – a term referring to informal investors – who are private wealthy individuals willing to invest a part of their own wealth in new business ventures for a profit. The business angel market is partially opaque in that many informal investors are not part of any organisation, club, or network and, therefore, they are difficult to identify (Mason and Harrison 2010; Mason and Harrison 2011). Many, however, are formally organised in clubs, associations, or networks (Amis and Stevenson 2001; Van Osnabrugge and Robinson 2000). Starting from the visible part of the market—the one linked by networks—and subsequent to the literature review, two relevant topics were identified: business angel profiles and their investment profiles. These two topics were crucial to the design of the questionnaires. Analysis of the questionnaire responses – which included the recipients of two of the most representative informal investor clubs in Portugal – helped to identify the profile of Portuguese business angels and their investments. Although the results of this research (detailed in Document 5: 3.2) could not be extrapolated to the entire Portuguese business angel community, the current research will offer future researchers another source in addition to the limited knowledge base available in this area
Interplay of LFV and slepton mass splittings at the LHC as a probe of the SUSY seesaw
We study the impact of a type-I SUSY seesaw concerning lepton flavour
violation (LFV) both at low-energies and at the LHC. The study of the di-lepton
invariant mass distribution at the LHC allows to reconstruct some of the masses
of the different sparticles involved in a decay chain. In particular, the
combination with other observables renders feasible the reconstruction of the
masses of the intermediate sleptons involved in decays. Slepton mass splittings can be either
interpreted as a signal of non-universality in the SUSY soft breaking-terms
(signalling a deviation from constrained scenarios as the cMSSM) or as being
due to the violation of lepton flavour. In the latter case, in addition to
these high-energy processes, one expects further low-energy manifestations of
LFV such as radiative and three-body lepton decays. Under the assumption of a
type-I seesaw as the source of neutrino masses and mixings, all these LFV
observables are related. Working in the framework of the cMSSM extended by
three right-handed neutrino superfields, we conduct a systematic analysis
addressing the simultaneous implications of the SUSY seesaw for both high- and
low-energy lepton flavour violation. We discuss how the confrontation of
slepton mass splittings as observed at the LHC and low-energy LFV observables
may provide important information about the underlying mechanism of LFV.Comment: 50 pages, 42 eps Figures, typos correcte
KSAC, a Defined Leishmania Antigen, plus Adjuvant Protects against the Virulence of L. major Transmitted by Its Natural Vector Phlebotomus duboscqi
Leishmaniasis is a neglected disease caused by the Leishmania parasite and transmitted by the bite of an infective sand fly. Despite the importance of this disease there is no vaccine available for humans. Studies have shown that vector-transmitted infections are more virulent, promoting parasite establishment and abrogating protection observed against needle-injected parasites in vaccinated mice. KSAC and L110f, derived from Leishmania-based polyproteins, protected mice against the needle-injected parasites. Here, we tested the two molecules for their capacity to protect mice against cutaneous leishmaniasis transmitted by an infective sand fly. Our results show that KSAC, but not L110f, confers protection against Leishmania transmitted by sand fly bites where protection was correlated to a strong immune response to Leishmania antigens by memory T cells before and after sand fly transmission of the parasite. This is the first report of a Leishmania-based vaccine that confers protection against a virulent sand fly challenge. Our results support the importance of screening Leishmania vaccine candidates using infective sand flies before moving forward with the costly steps of vaccine development
In vitro activity of daptomycin, linezolid and rifampicin on Staphylococcus epidermidis biofilms
Owing to their massive use, Staphylococcus
epidermidis has recently developed significant resistance to
several antibiotics, and became one of the leading causes of
hospital-acquired infections. Current antibiotics are typically
ineffective in the eradication of bacteria in biofilmassociated
persistent infections. Accordingly, the paucity
of effective treatment against cells in this mode of growth
is a key factor that potentiates the need for new agents
active in the prevention or eradication of biofilms. Daptomycin
and linezolid belong to the novel antibiotic therapies
that are active against gram-positive cocci. On the other
hand, rifampicin has been shown to be one of the most
potent, prevalent antibiotics against S. epidermidis biofilms.
Therefore, the main aim of this study was to study
the susceptibility of S. epidermidis biofilm cells to the two
newer antimicrobial agents previously mentioned, and
compare the results obtained with the antimicrobial effect
of rifampicin, widely used in the prevention/treatment of
indwelling medical device infections. To this end the in
vitro activities of daptomycin, linezolid, and rifampicin on
S. epidermidis biofilms were accessed, using these antibiotics
at MIC and peak serum concentrations. The results
demonstrated that at MIC concentration, rifampicin was the
most effective antibiotic tested. At peak serum concentration,
both strains demonstrated similar susceptibility to
rifampicin and daptomycin, with colony-forming units
(CFUs) reductions of approximately 3–4 log10, with a
slightly lower response to linezolid, which was also more
strain dependent. However, considering all the parameters
studied, daptomycin was considered the most effective
antibiotic tested, demonstrating an excellent in vitro
activity against S. epidermidis biofilm cells. In conclusion,
this antibiotic can be strongly considered as an acceptable
therapeutic option for S. epidermidis biofilm-associated
infections and can represent a potential alternative to rifampicin
in serious infections where rifampicin resistance
becomes prevalent.Bruna Leite acknowledges the financial support from ISAC/Program Erasmus Munds External Cooperation and the IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus of Gualtar. Fernanda Gomes and Pilar Teixeira fully acknowledge the financial support from Fundacao para a Ciencia e Tecnologia (FCT) through the grants SFRH/BD/32126/2006 and SFRH/BPD/26803/2006, respectively
The effect of the electric field on lag phase, β-galactosidase production and plasmid stability of a recombinant Saccharomyces cerevisiae strain growing on lactose
Ethanol and β-galactosidase production from cheese whey may significantly contribute to minimise environmental problems while producing value from lowcost raw materials. In this work, the recombinant Saccharomyces cerevisiae NCYC869-A3/pVK1.1 flocculent strain expressing the lacA gene (coding for β-galactosidase) of Aspergillus niger under ADHI promoter and terminator was used. This strain shows high ethanol and β-galactosidase productivities when grown on lactose. Batch cultures were performed using SSlactose medium with 50 gL−1 lactose in a 2-L bioreactor under aerobic and microaerophilic conditions. Temperature was maintained at 30 °C and pH 4.0. In order to determine the effect of an electric field in the fermentation profile, titanium electrodes were placed inside the bioreactor and different electric field values (from 0.5 to 2 Vcm−1) were applied. For all experiments, β-galactosidase activity, biomass, protein, lactose, glucose, galactose and ethanol concentrations were measured. Finally, lag phase duration and specific growth rate were calculated. Significant changes in lag phase duration and biomass yield were found when using 2 Vcm−1. Results show that the electric field enhances the early stages of fermentation kinetics, thus indicating that its application may improve industrial fermentations’ productivity. The increase in electric field intensity led to plasmid instability thus decreasing β-galactosidase production.The authors gratefully acknowledge Fundacao para a Ciencia e a Tecnologia (Portugal) for the scholarships SFRH/BD/11230/2002 and SFRH/BDP/63831/2009 granted to authors I. Castro and C. Oliveira, respectively
Global variation in the cost of increasing ecosystem carbon
Slowing the reduction, or increasing the accumulation, of organic carbon stored in biomass and soils has been suggested as a potentially rapid and cost-effective method to reduce the rate of atmospheric carbon increase(1). The costs of mitigating climate change by increasing ecosystem carbon relative to the baseline or business-as-usual scenario has been quantified in numerous studies, but results have been contradictory, as both methodological issues and substance differences cause variability(2). Here we show, based on 77 standardized face-to-face interviews of local experts with the best possible knowledge of local land-use economics and sociopolitical context in ten landscapes around the globe, that the estimated cost of increasing ecosystem carbon varied vastly and was perceived to be 16-27 times cheaper in two Indonesian landscapes dominated by peatlands compared with the average of the eight other landscapes. Hence, if reducing emissions from deforestation and forest degradation (REDD+) and other land-use mitigation efforts are to be distributed evenly across forested countries, for example, for the sake of international equity, their overall effectiveness would be dramatically lower than for a cost-minimizing distribution.Peer reviewe
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