Microbial Communities in a High Arctic Polar Desert Landscape

The High Arctic is dominated by polar desert habitats whose microbial communities are poorly understood. In this study, we used next generation sequencing to describe the α- and β-diversity of microbial communities in polar desert soils from the Kongsfjorden region of Svalbard. Ten phyla dominated the soils and accounted for 95% of all sequences, with the Proteobacteria, Actinobacteria, and Chloroflexi being the major lineages. In contrast to previous investigations of Arctic soils, relative Acidobacterial abundances were found to be very low as were the Archaea throughout the Kongsfjorden polar desert landscape. Lower Acidobacterial abundances were attributed to characteristic circumneutral soil pHs in this region, which has resulted from the weathering of underlying carbonate bedrock. In addition, we compared previously measured geochemical conditions as possible controls on soil microbial communities. Phosphorus, pH, nitrogen, and calcium levels all significantly correlated with β-diversity, indicating landscape-scale lithological control of available nutrients, which in turn, significantly influenced soil community composition. In addition, soil phosphorus and pH significantly correlated with α-diversity, particularly with the Shannon diversity and Chao 1 richness indices

Effect of different grazing pressure by lambs grazing Lolium perenne and Dactylis glomerata pastures during spring on: 1. Diet quality

This study was undertaken to determine the influence of three grazing pressures [high (HGP), medium (MGP) and low (LGP), corresponding to 30, 50 and 75 g available DM/kg BW/day, respectively] on the quality of herbage consumed by lambs grazing Lolium perenne and Dactylis glomerata pastures in spring. Feed samples collected via oesophageal fistulae at the start and end of a six-week grazing period were analyzed for nitrogen (N), ash, neutral detergent fibre (NDF), acid detergent fibre (ADF), acid detergent lignin (ADL) concentrations, and in vitro digestibility of organic matter (IVDOM). In general, the quality of diets selected by lambs grazing both L. perenne and D. glomerata pastures was lower at the end than at the start of the grazing period. For both pastures the N concentration of herbage consumed by lambs in the HGP treatment was lower than for that in the LGP treatment. For L. perenne HGP resulted in the selection of a diet with higher fibre (NDF and ADF) concentrations and consequently lower IVDOM than in the LGP treatment. In the D. glomerata pasture, higher ADF and lower IVDOM were recorded in the MGP treatment compared to the other treatments. On the D. glomerata pasture the ADL concentration of the selected forage was lower in the HGP than in the MGP or LGP treatments. Forage selected on the L. perenne pasture had generally lower N, ash, ADF and ADL concentrations than those selected from the D. glomerata pasture. However, the IVDOM of the selected L. perenne was higher than that of selected D. glomerata. Keywords: Cocksfoot; chemical composition; diet selection; pasture availability; perennial rye grass South African Journal of Animal Science Vol. 36 (5) 2006: pp.46-4

Effect of different grazing pressures by lambs grazing Lolium perenne and Dactylis glomerata pastures during spring on: 2. Intake and growth

This study was undertaken to determine the influence of three grazing pressures [high (HGP), medium (MGP) and low (LGP), corresponding to 30, 50 and 75 g available DM/kg BW/day, respectively] on the performance of lambs grazing Lolium perenne and Dactylis glomerata pastures in spring. Feed intakes and average daily live weight gains (ADG in g/day) were determined for each group. In general intakes of both L. perenne and D. glomerata pastures were higher at the end of the study than at the onset. Grazing pressure had no effect on the intake of L. perenne, but on the D. glomerata pasture, HGP resulted in a decreased intake by the lambs compared to the LGP treatment. For both pasture species the ADG of the lambs were lower on the HGP compared to the MGP treatment, though intake and ADG of the lambs were higher when grazing L. perenne compared to D. glomerata. Keywords:Cocksfoot; diet selection; pasture availability; perennial rye grass; performance South African Journal of Animal Science Vol. 36 (5) 2006: pp.50-5

Growth performance and carcass characteristics of three Ethiopian goat breeds fed grainless diets varying in concentrate to roughage ratios

Growth and carcass characteristics of three Ethiopian goat breeds, the Afar, Central Highland (CHG) and Long-eared Somali (LES) were evaluated using three grainless diets varying in concentrate:roughage ratios (diet 1 was 50:50, diet 2, 65:35 and diet 3, 80:20) under feedlot conditions. The roughage was native grass hay and the concentrate consisted of wheat bran and noug cake (Guizotia abyssinica). Seventy-two eight-month old intact male goats (24 per breed) were randomly allotted to the dietary treatments, fed for 126 days and slaughtered at an age of approximately 12 months. The LES had higher average daily gain (ADG), heavier slaughter, empty body (EBW) and carcass weights than Afar and CHG goats. Diet significantly affected ADG, but was similar on carcass traits except for dressing percentage (DP) on an EBW basis and some non-carcass components. The DP on an EBW basis was the highest on diet 1. Breed affected the DP, which ranged from 42.5 - 44.6% and 54.3 - 55.8% on slaughter weight and EBW basis, respectively. The LES had a greater buttock circumference and carcass compactness. The pH24 varied between 5.61 - 5.67 and chilling losses were between 2.5 and 3.1%. The physical carcass composition (8-10th rib-cut) ranged from 72 - 73, 6.9 - 10.9 and 17.1 - 20.2% for lean, fat and bone, respectively, and the fat content of the meat ranged from 10.3 - 14.0%. Breed affected the weights of internal fat depots. The findings indicate that breed affected the carcass characteristics of the three Ethiopian goat breeds. Keywords: Indigenous goats; carcass yield; carcass composition; primal cuts; non-carcass components South African Journal of Animal Science Vol. 37 (4) 2007: pp.221-23

The Interstellar Medium In Galaxies Seen A Billion Years After The Big Bang

Evolution in the measured rest frame ultraviolet spectral slope and ultraviolet to optical flux ratios indicate a rapid evolution in the dust obscuration of galaxies during the first 3 billion years of cosmic time (z>4). This evolution implies a change in the average interstellar medium properties, but the measurements are systematically uncertain due to untested assumptions, and the inability to measure heavily obscured regions of the galaxies. Previous attempts to directly measure the interstellar medium in normal galaxies at these redshifts have failed for a number of reasons with one notable exception. Here we report measurements of the [CII] gas and dust emission in 9 typical (~1-4L*) star-forming galaxies ~1 billon years after the big bang (z~5-6). We find these galaxies have >12x less thermal emission compared with similar systems ~2 billion years later, and enhanced [CII] emission relative to the far-infrared continuum, confirming a strong evolution in the interstellar medium properties in the early universe. The gas is distributed over scales of 1-8 kpc, and shows diverse dynamics within the sample. These results are consistent with early galaxies having significantly less dust than typical galaxies seen at z<3 and being comparable to local low-metallicity systems.Comment: Submitted to Nature, under review after referee report. 22 pages, 4 figures, 4 Extended Data Figures, 5 Extended Data table

Core Depressive Symptoms In Depressed Cancer OutpatientsB

Objective: This study aimed to estimate the prevalence of core depressive symptoms among cancer outpatients diagnosed with depressive or adjustment disorders with depressed mood. We also aimed to detect potential differences between patient self-assessment and psychiatrist evaluation in classifying the severity of depression. Methods: Fifty-two outpatients diagnosed with solid tumor malignancy and depressive or adjustment disorder with depressed mood were assessed using the Hamilton Depression Rating Scale (HAMD-17) (and its shortened version the HAMD-7) and the Zung Self-Rating Depression Scale (ZSDS) (and its shortened version BZSDS). Results: Based on HAMD-7 results, the prevalence of moderate depression was low (7.7%); using the BZSDS moderate depression was absent. Mild depression was identified in 82.3% and 73% of our subjects using the HAMD-7 and the BZSDS, respectively. The strength of agreement between psychiatrist and patients' self-evaluation for mild depression was "slight", employing the original and the abbreviated versions of both scales. Conclusion: Our findings suggest that the prevalence of core depressive symptoms is very low in cancer patients diagnosed with depressive disorder. The lack of a strong agreement between psychiatrist and patient in classifying the severity of depression highlights the importance of factors such as well-being and functional status among depressed cancer patients in their self assessment of depression. © Massimo et al

"First pain" in humans: convergent and specific forebrain responses

<p>Abstract</p> <p>Background</p> <p>Brief heat stimuli that excite nociceptors innervated by finely myelinated (Aδ) fibers evoke an initial, sharp, well-localized pain ("first pain") that is distinguishable from the delayed, less intense, more prolonged dull pain attributed to nociceptors innervated by unmyelinated (C) fibers ("second pain"). In the present study, we address the question of whether a brief, noxious heat stimulus that excites cutaneous Aδ fibers activates a distinct set of forebrain structures preferentially in addition to those with similar responses to converging input from C fibers. Heat stimuli at two temperatures were applied to the dorsum of the left hand of healthy volunteers in a functional brain imaging (fMRI) paradigm and responses analyzed in a set of volumes of interest (VOI).</p> <p>Results</p> <p>Brief 41°C stimuli were painless and evoked only C fiber responses, but 51°C stimuli were at pain threshold and preferentially evoked Aδ fiber responses. Most VOI responded to both intensities of stimulation. However, within volumes of interest, a contrast analysis and comparison of BOLD response latencies showed that the bilateral anterior insulae, the contralateral hippocampus, and the ipsilateral posterior insula were preferentially activated by painful heat stimulation that excited Aδ fibers.</p> <p>Conclusions</p> <p>These findings show that two sets of forebrain structures mediate the initial sharp pain evoked by brief cutaneous heat stimulation: those responding preferentially to the brief stimulation of Aδ heat nociceptors and those with similar responses to converging inputs from the painless stimulation of C fibers. Our results suggest a unique and specific physiological basis, at the forebrain level, for the "first pain" sensation that has long been attributed to Aδ fiber stimulation and support the concept that both specific and convergent mechanisms act concurrently to mediate pain.</p

Mitochondrial phylogeography and demographic history of the Vicuña: implications for conservation

The vicuña (Vicugna vicugna; Miller, 1924) is a conservation success story, having recovered from near extinction in the 1960s to current population levels estimated at 275 000. However, lack of information about its demographic history and genetic diversity has limited both our understanding of its recovery and the development of science-based conservation measures. To examine the evolution and recent demographic history of the vicuña across its current range and to assess its genetic variation and population structure, we sequenced mitochondrial DNA from the control region (CR) for 261 individuals from 29 populations across Peru, Chile and Argentina. Our results suggest that populations currently designated as Vicugna vicugna vicugna and Vicugna vicugna mensalis comprise separate mitochondrial lineages. The current population distribution appears to be the result of a recent demographic expansion associated with the last major glacial event of the Pleistocene in the northern (18 to 22°S) dry Andes 14–12 000 years ago and the establishment of an extremely arid belt known as the 'Dry Diagonal' to 29°S. Within the Dry Diagonal, small populations of V. v. vicugna appear to have survived showing the genetic signature of demographic isolation, whereas to the north V. v. mensalis populations underwent a rapid demographic expansion before recent anthropogenic impacts

Long-Term Weekly Iron-Folic Acid and De-Worming Is Associated with Stabilised Haemoglobin and Increasing Iron Stores in Non-Pregnant Women in Vietnam

BACKGROUND: The prevalence of anaemia and iron deficiency in women remains high worldwide. WHO recommends weekly iron-folic acid supplementation where anaemia rates in non-pregnant women of reproductive age are higher than 20%. In 2006, a demonstration project consisting of weekly iron-folic acid supplementation and regular de-worming was set up in two districts in a northern province in Vietnam where anaemia and hookworm rates were 38% and 76% respectively. In 2008 the project was expanded to all districts in the province, targeting some 250,000 women. The objectives of this study were to: 1) examine changes in haemoglobin, iron stores and soil transmitted helminth infection prevalence over three years and 2) assess women's access to and compliance with the intervention. METHODS AND FINDINGS: The study was a semi-cross-sectional, semi-longitudinal panel design with a baseline survey, three impact surveys at three-, twelve- and thirty months after commencement of the intervention, and three compliance surveys after ten weeks, eighteen and thirty six months. RESULTS: After thirty months, mean haemoglobin stabilised at 130.3 g/L, an increase of 8.2 g/L from baseline, and mean serum ferritin rose from 23.9 µg/L to 52 µg/L. Hookworm prevalence fell from 76% to 22% over the same period. After thirty six months, 81% of the target population were receiving supplements and 87% were taking 75% or more of the supplements they received. CONCLUSIONS: Weekly iron-folic acid supplementation and regular de-worming was effective in significantly and sustainably reducing the prevalence of anaemia and soil transmitted helminth infections and high compliance rates were maintained over three years