Enhanced Partial Tracking Using Linear Prediction

International audienceIn this paper, we introduce a new partial tracking method suitable for the sinusoidal modeling of mixtures of instrumental sounds with pseudo stationary frequencies. This method, based on the linear prediction of the frequency evolutions of the partials, enables us to track these partials more accurately at the analysis stage, even in complex sound mixtures. This allows our spectral model to better handle polyphonic sound

Proposal of a quantitative PCR-based protocol for an optimal Pseudomonas aeruginosa detection in patients with cystic fibrosis

BACKGROUND: The lung of patients with cystic fibrosis (CF) is particularly sensitive to Pseudomonas aeruginosa. This bacterium plays an important role in the poor outcome of CF patients. During the disease progress, first acquisition of P. aeruginosa is the key-step in the management of CF patients. Quantitative PCR (qPCR) offers an opportunity to detect earlier the first acquisition of P. aeruginosa by CF patients. Given the lack of a validated protocol, our goal was to find an optimal molecular protocol for detection of P. aeruginosa in CF patients. METHODS: We compared two formerly described qPCR formats in early detection of P. aeruginosa in CF sputum samples: a qPCR targeting oprL gene, and a multiplex PCR targeting gyrB and ecfX genes. RESULTS: Tested in vitro on a large panel of P. aeruginosa isolates and others gram-negative bacilli, oprL qPCR exhibited a better sensitivity (threshold of 10 CFU/mL versus 730 CFU/mL), whereas the gyrB/ecfX qPCR exhibited a better specificity (90% versus 73%). These results were validated ex vivo on 46 CF sputum samples positive for P. aeruginosa in culture. Ex vivo assays revealed that qPCR detected 100 times more bacterial cells than culture-based method did. CONCLUSION: Based on these results, we proposed a reference molecular protocol combining the two qPCRs, which offers a sensitivity of 100% with a threshold of 10 CFU/mL and a specificity of 100%. This combined qPCR-based protocol can be adapted and used for other future prospective studies

An inter­molecular dative B←N bond in 5-(4,4,5,5-tetra­methyl-1,3,2-dioxa­borolan-2-yl)-1,3-thia­zole

The title compound, C9H14BNO2S, is in an unusual bend conformation and the B atom of one mol­ecule within the crystal forms an inter­molecular dative bond with the N atom of a neighbouring mol­ecule, an infrequent phenomenon in boronic derivative crystals

tert-Butyl 6-bromo-1,4-dimethyl-9H-carbazole-9-carboxyl­ate

The title compound, C19H20BrNO2, consists of a carbazole skeleton with methyl groups at positions 1 and 4, a protecting group located at the N atom and a Br atom at position 6. The pyrrole ring is oriented at dihedral angles of 1.27 (7) and 4.86 (7)° with respect to the adjacent benzene rings. The dihedral angle between the benzene rings is 5.11 (7). The crystal structure is determined mainly by intra­molecular C—H⋯O and inter­molecular π–π inter­actions. π-stacking between adjacent molecules forms columns with a parallel arrangement of the carbazole ring systems. The presence of the tert-but­oxy­carbonyl group on the carbazole N atom and the intra­molecular hydrogen bond induce a particular conformation of the exocyclic N—C bond within the mol­ecule

Etude comparative de l'inactivation des amibes libres du genre Naegleria par voie chimique et physique

CAEN-BU Médecine pharmacie (141182102) / SudocLYON1-BU Santé (693882101) / SudocSudocFranceF

Objets en verre à usage pharmaceutique (inventaire centré sur le XIXe siècle)

CAEN-BU Médecine pharmacie (141182102) / SudocLYON1-BU Santé (693882101) / SudocSudocFranceF

Synthèse de nouvelles thiénoimidazolones à visée sérotoninergique

CAEN-BU Médecine pharmacie (141182102) / SudocLYON1-BU Santé (693882101) / SudocSudocFranceF