1,664 research outputs found
RNomics: a computational search for box C/D snoRNA genes in the D. melanogaster genome.
Motivation: In eukaryotes, the family of non-coding RNA
genes includes a number of genes encoding small nucleolar
RNAs (mainly C/D and H/ACA snoRNAs), which act as
guides in the maturation or post-transcriptional modifications
of target RNA molecules. Since in Drosophila melanogaster
(Dm) only few examples of snoRNAs have been identified so
far by cDNA libraries screening, integration of the molecular
data with in silico identification of these types of genes could
throw light on their organization in the Dm genome.
Results: We have performed a computational screening of
the Dm genome for C/D snoRNA genes, followed by experimental validation of the putative candidates. Few of the
26 confirmed snoRNAs had been recognized by cDNA library
analysis. Organization of the Dm genome was also
found to be more variegated than previously suspected, with
snoRNA genes nested in both the introns and exons of
protein-coding genes. This finding suggests that the presence
of additional mechanisms of snoRNA biogenesis based
on the alternative production of overlapping mRNA/snoRNA
molecules.
Availability: Additional information is available at http://www.
bioinformatica.unito.it/bioinformatics/snoRNA
Wilson-loop formalism for Reggeon exchange in soft high-energy scattering
We derive a nonperturbative expression for the non-vacuum,
qqbar-Reggeon-exchange contribution to the meson-meson elastic scattering
amplitude at high energy and low momentum transfer, in the framework of QCD.
Describing the mesons in terms of colourless qqbar dipoles, the problem is
reduced to the two-fermion-exchange contribution to the dipole-dipole
scattering amplitudes, which is expressed as a path integral, over the
trajectories of the exchanged fermions, of the expectation value of a certain
Wilson loop. We also show how the resulting expression can be reconstructed
from a corresponding quantity in the Euclidean theory, by means of analytic
continuation. Finally, we make contact with previous work on Reggeon exchange
in the gauge/gravity duality approach.Comment: A few misprints in the expressions for the relevant Wilson loops have
been corrected. 55 pages, 7 figure
Reggeon exchange from gauge/gravity duality
We perform the analysis of quark-antiquark Reggeon exchange in meson-meson
scattering, in the framework of the gauge/gravity correspondence in a confining
background. On the gauge theory side, Reggeon exchange is described as
quark-antiquark exchange in the t channel between fast projectiles. The
corresponding amplitude is represented in terms of Wilson loops running along
the trajectories of the constituent quarks and antiquarks. The paths of the
exchanged fermions are integrated over, while the "spectator" fermions are
dealt with in an eikonal approximation. On the gravity side, we follow a
previously proposed approach, and we evaluate the Wilson-loop expectation value
by making use of gauge/gravity duality for a generic confining gauge theory.
The amplitude is obtained in a saddle-point approximation through the
determination near the confining horizon of a Euclidean "minimal surface with
floating boundaries", i.e., by fixing the trajectories of the exchanged quark
and antiquark by means of a minimisation procedure, which involves both area
and length terms. After discussing, as a warm-up exercise, a simpler problem on
a plane involving a soap film with floating boundaries, we solve the
variational problem relevant to Reggeon exchange, in which the basic geometry
is that of a helicoid. A compact expression for the Reggeon-exchange amplitude,
including the effects of a small fermion mass, is then obtained through
analytic continuation from Euclidean to Minkowski space-time. We find in
particular a linear Regge trajectory, corresponding to a Regge-pole singularity
supplemented by a logarithmic cut induced by the non-zero quark mass. The
analytic continuation leads also to companion contributions, corresponding to
the convolution of the same Reggeon-exchange amplitude with multiple elastic
rescattering interactions between the colliding mesons.Comment: 60+1 pages, 14 figure
High Energy Bounds on Soft N=4 SYM Amplitudes from AdS/CFT
Using the AdS/CFT correspondence, we study the high-energy behavior of
colorless dipole elastic scattering amplitudes in N=4 SYM gauge theory through
the Wilson loop correlator formalism and Euclidean to Minkowskian analytic
continuation. The purely elastic behavior obtained at large impact-parameter L,
through duality from disconnected AdS_5 minimal surfaces beyond the
Gross-Ooguri transition point, is combined with unitarity and analyticity
constraints in the central region. In this way we obtain an absolute bound on
the high-energy behavior of the forward scattering amplitude due to the
graviton interaction between minimal surfaces in the bulk. The dominant
"Pomeron" intercept is bounded by alpha less than or equal to 11/7 using the
AdS/CFT constraint of a weak gravitational field in the bulk. Assuming the
elastic eikonal approximation in a larger impact-parameter range gives alpha
between 4/3 and 11/7. The actual intercept becomes 4/3 if one assumes the
elastic eikonal approximation within its maximally allowed range L larger than
exp{Y/3}, where Y is the total rapidity. Subleading AdS/CFT contributions at
large impact-parameter due to the other d=10 supergravity fields are obtained.
A divergence in the real part of the tachyonic KK scalar is cured by
analyticity but signals the need for a theoretical completion of the AdS/CFT
scheme.Comment: 25 pages, 3 eps figure
Expression of Protease-Activated Receptor 1 and 2 and Anti-Tubulogenic Activity of Protease-Activated Receptor 1 in Human Endothelial Colony-Forming Cells
Endothelial colony-forming cells (ECFCs) are obtained from the culture of human peripheral blood mononuclear cell (hPBMNC) fractions and are characterised by high proliferative and pro-vasculogenic potential, which makes them of great interest for cell therapy. Here, we describe the detection of protease-activated receptor (PAR) 1 and 2 amongst the surface proteins expressed in ECFCs. Both receptors are functionally coupled to extracellular signal-regulated kinase (ERK) 1 and 2, which become activated and phosphorylated in response to selective PAR1- or PAR2-activating peptides. Specific stimulation of PAR1, but not PAR2, significantly inhibits capillary-like tube formation by ECFCs in vitro, suggesting that tubulogenesis is negatively regulated by proteases able to stimulate PAR1 (e.g. thrombin). The activation of ERKs is not involved in the regulation of tubulogenesis in vitro, as suggested by use of the MEK inhibitor PD98059 and by the fact that PAR2 stimulation activates ERKs without affecting capillary tube formation. Both qPCR and immunoblotting showed a significant downregulation of vascular endothelial growth factor 2 (VEGFR2) in response to PAR1 stimulation. Moreover, the addition of VEGF (50–100 ng/ml) but not basic Fibroblast Growth Factor (FGF) (25–100 ng/ml) rescued tube formation by ECFCs treated with PAR1-activating peptide. Therefore, we propose that reduction of VEGF responsiveness resulting from down-regulation of VEGFR2 is underlying the anti-tubulogenic effect of PAR1 activation. Although the role of PAR2 remains elusive, this study sheds new light on the regulation of the vasculogenic activity of ECFCs and suggests a potential link between adult vasculogenesis and the coagulation cascade
Adherence to self-administered tuberculosis treatment in a high HIV-prevalence setting: a cross-sectional survey in Homa Bay, Kenya.
Good adherence to treatment is crucial to control tuberculosis (TB). Efficiency and feasibility of directly observed therapy (DOT) under routine program conditions have been questioned. As an alternative, Médecins sans Frontières introduced self-administered therapy (SAT) in several TB programs. We aimed to measure adherence to TB treatment among patients receiving TB chemotherapy with fixed dose combination (FDC) under SAT at the Homa Bay district hospital (Kenya). A second objective was to compare the adherence agreement between different assessment tools
Non-standard management of breast cancer increases with age in the UK: a population based cohort of women ⩾65 years
Evidence suggests that compared to younger women, older women are less likely to receive standard management for breast cancer. Whether this disparity persists once differences in tumour characteristics have been adjusted for has not been investigated in the UK. A retrospective cohort study involving case note review was undertaken, based on the North Western Cancer Registry database of women aged ⩾65 years, resident in Greater Manchester with invasive breast cancer registered over a 1-year period (n=480). Adjusting for tumour characteristics associated with age by logistic regression analyses, older women were less likely to receive standard management than younger women for all indicators investigated. Compared to women aged 65–69 years, women aged ⩾80 years with operable (stage 1–3a) breast cancer have increased odds of not receiving triple assessment (OR=5.5, 95% confidence interval (CI): 2.1–14.5), not receiving primary surgery (OR=43.0, 95% CI: 9.7–191.3), not undergoing axillary node surgery (OR=27.6, 95% CI: 5.6–135.9) and not undergoing tests for steroid receptors (OR=3.0, 95% CI: 1.7–5.5). Women aged 75–79 years have increased odds of not receiving radiotherapy following breast-conserving surgery compared to women aged 65–69 years (OR=11.0, 95% CI: 2.0–61.6). These results demonstrate that older women in the UK are less likely to receive standard management for breast cancer, compared to younger women and this disparity cannot be explained by differences in tumour characteristics
Radio emission from Supernova Remnants
The explosion of a supernova releases almost instantaneously about 10^51 ergs
of mechanic energy, changing irreversibly the physical and chemical properties
of large regions in the galaxies. The stellar ejecta, the nebula resulting from
the powerful shock waves, and sometimes a compact stellar remnant, constitute a
supernova remnant (SNR). They can radiate their energy across the whole
electromagnetic spectrum, but the great majority are radio sources. Almost 70
years after the first detection of radio emission coming from a SNR, great
progress has been achieved in the comprehension of their physical
characteristics and evolution. We review the present knowledge of different
aspects of radio remnants, focusing on sources of the Milky Way and the
Magellanic Clouds, where the SNRs can be spatially resolved. We present a brief
overview of theoretical background, analyze morphology and polarization
properties, and review and critical discuss different methods applied to
determine the radio spectrum and distances. The consequences of the interaction
between the SNR shocks and the surrounding medium are examined, including the
question of whether SNRs can trigger the formation of new stars. Cases of
multispectral comparison are presented. A section is devoted to reviewing
recent results of radio SNRs in the Magellanic Clouds, with particular emphasis
on the radio properties of SN 1987A, an ideal laboratory to investigate
dynamical evolution of an SNR in near real time. The review concludes with a
summary of issues on radio SNRs that deserve further study, and analyzing the
prospects for future research with the latest generation radio telescopes.Comment: Revised version. 48 pages, 15 figure
CLU blocks HDACI-mediated killing of neuroblastoma
Clusterin is a ubiquitously expressed glycoprotein with multiple binding partners including IL-6, Ku70, and Bax. Clusterin blocks apoptosis by binding to activated Bax and sequestering it in the cytoplasm, thereby preventing Bax from entering mitochondria, releasing cytochrome c, and triggering apoptosis. Because increased clusterin expression correlates with aggressive behavior in tumors, clusterin inhibition might be beneficial in cancer treatment. Our recent findings indicated that, in neuroblastoma cells, cytoplasmic Bax also binds to Ku70; when Ku70 is acetylated, Bax is released and can initiate cell death. Therefore, increasing Ku70 acetylation, such as by using histone deacetylase inhibitors, may be therapeutically useful in promoting cell death in neuroblastoma tumors. Since clusterin, Bax, and Ku70 form a complex, it seemed likely that clusterin would mediate its anti-apoptotic effects by inhibiting Ku70 acetylation and blocking Bax release. Our results, however, demonstrate that while clusterin level does indeed determine the sensitivity of neuroblastoma cells to histone deacetylase inhibitor-induced cell death, it does so without affecting histone deacetylase-inhibitor-induced Ku70 acetylation. Our results suggest that in neuroblastoma, clusterin exerts its anti-apoptotic effects downstream of Ku70 acetylation, likely by directly blocking Bax activation
Perspectives on the Trypanosoma cruzi-host cell receptor interaction
Chagas disease is caused by the parasite Trypanosoma cruzi. The critical initial event is the interaction of the trypomastigote form of the parasite with host receptors. This review highlights recent observations concerning these interactions. Some of the key receptors considered are those for thromboxane, bradykinin, and for the nerve growth factor TrKA. Other important receptors such as galectin-3, thrombospondin, and laminin are also discussed. Investigation into the molecular biology and cell biology of host receptors for T. cruzi may provide novel therapeutic targets
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