Quantitative profiling of feruloylated arabinoxylan side-chains from graminaceous cell walls

Graminaceous arabinoxylans are distinguished by decoration with feruloylated monosaccharidic and oligosaccharidic side-chains. Although it is hypothesized that structural complexity and abundance of these feruloylated arabinoxylan side-chains may contribute, among other factors, to resistance of plant cell walls to enzymatic degradation, quantitative profiling approaches for these structural units in plant cell wall materials have not been described yet. Here we report the development and application of a rapid and robust method enabling the quantitative comparison of feruloylated side-chain profiles in cell wall materials following mildly acidic hydrolysis, C18-solid phase extraction (SPE), reduction under aprotic conditions, and liquid chromatography with diode array detection/mass spectrometry (LC-DAD/MS) separation and detection. The method was applied to the insoluble fiber/cell wall materials isolated from 12 whole grains: wild rice (Zizania aquatica L.), long-grain brown rice (Oryza sativa L.), rye (Secale cereale L.), kamut (Triticum turanicum Jakubz.), wheat (Triticum aestivum L.), spelt (Triticum spelta L.), intermediate wheatgrass (Thinopyrum intermedium), maize (Zea mays L.), popcorn (Zea mays L. var. everta), oat (Avena sativa L.) (dehulled), barley (Hordeum vulgare L.) (dehulled), and proso millet (Panicum miliaceum L.). Between 51 and 96% of the total esterified monomeric ferulates were represented in the quantified compounds captured in the feruloylated side-chain profiles, which confirms the significance of these structures to the global arabinoxylan structure in terms of quantity. The method provided new structural insights into cereal grain arabinoxylans, in particular, that the structural moiety α-L-galactopyranosyl (1→2)-β-D-xylopyranosyl-(1→2)-5-O-trans-feruloyl-L-arabinofuranose (FAXG), which had previously only been described in maize, is ubiquitous to cereal grains. © 2016 Schendel, Meyer and Bunzel

Inner ear tissue preservation by rapid freezing: improving fixation by high-pressure freezing and hybrid methods

In the preservation of tissues in as ‘close to life’ state as possible, rapid freeze fixation has many benefits over conventional chemical fixation. One technique by which rapid freeze-fixation can be achieved, high pressure freezing (HPF), has been shown to enable ice crystal artefact-free freezing and tissue preservation to greater depths (more than 40μm) than other quick-freezing methods. Despite increasingly becoming routine in electron microscopy, the use of HPF for the fixation of inner ear tissue has been limited. Assessment of the quality of preservation showed routine HPF techniques were suitable for preparation of inner ear tissues in a variety of species. Good preservation throughout the depth of sensory epithelia was achievable. Comparison to chemically fixed tissue indicated that fresh frozen preparations exhibited overall superior structural preservation of cells. However, HPF fixation caused characteristic artefacts in stereocilia that suggested poor quality freezing of the actin bundles. The hybrid technique of pre-fixation and high pressure freezing was shown to produce cellular preservation throughout the tissue, similar to that seen in HPF alone. Pre-fixation HPF produced consistent high quality preservation of stereociliary actin bundles. Optimising the preparation of samples with minimal artefact formation allows analysis of the links between ultrastructure and function in inner ear tissues

Six-dimensional Abelian vortices with quadratic curvature self-interactions

Six-dimensional Nielsen-Olesen vortices are analyzed in the context of a quadratic gravity theory containing Euler-Gauss-Bonnet self-interactions. The relations among the string tensions can be tuned in such a way that the obtained solutions lead to warped compactification on the vortex. New regular solutions are possible in comparison with the case where the gravity action only consists of the Einstein-Hilbert term. The parameter space of the model is discussedComment: 28 pages in Latex style with 11 figure

Red cell ABO incompatibility and production of tumour necrosis factor-alpha

Tumour necrosis factor-alpha (TNF) is a major mediator of diverse pathophysiological events similar to those of haemolytic transfusion reactions (HTR), such as fever, intravascular coagulation and organ failure. However, the possible role of TNF in HTR has not been investigated. We have constructed an in vitro whole blood model of HTR to examine whether TNF may be produced in red cell ABO incompatibility. TNF was observed in plasma, in a dose dependent manner, when ABO incompatible red cells were added, but not with compatible (group O) cells. Plasma TNF levels were maximal at 2 h, and declined to control levels by 24 h. Haemolysis of incompatible red cells was accompanied by TNF production. Immune haemolysis induced TNF gene expression by buffy coat leucocytes, as determined by Northern blot analysis. Heat inactivation of plasma abolished TNF production, whereas prior treatment with interferongamma augmented the response. These results demonstrate that a major cytokine is produced in response to red cell incompatibility, and suggest that TNF may play a role in the pathogenesis of haemolytic transfusion reactions.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75627/1/j.1365-2141.1991.tb04485.x.pd

Statistical Survey of Type III Radio Bursts at Long Wavelengths Observed by the Solar TErrestrial RElations Observatory (STEREO)/Waves Instruments: Radio Flux Density Variations with Frequency

We have performed a statistical study of $152$ Type III radio bursts observed by Solar TErrestrial RElations Observatory (STEREO)/Waves between May 2007 and February 2013. We have investigated the flux density between $125$kHz and $16$MHz. Both high- and low-frequency cutoffs have been observed in $60\,$ of events suggesting an important role of propagation. As already reported by previous authors, we observed that the maximum flux density occurs at $1$MHz on both spacecraft. We have developed a simplified analytical model of the flux density as a function of radial distance and compared it to the STEREO/Waves data.Comment: published in Solar Physic

Influence of a classical homogeneous gravitational field on dissipative dynamics of the Jaynes-Cummings model with phase damping

In this paper, we study the dissipative dynamics of the Jaynes-Cummings model with phase damping in the presence of a classical homogeneous gravitational field. The model consists of a moving two-level atom simultaneously exposed to the gravitational field and a single-mode traveling radiation field in the presence of the phase damping. We present a quantum treatment of the internal and external dynamics of the atom based on an alternative su(2) dynamical algebraic structure. By making use of the super-operator technique, we obtain the solution of the master equation for the density operator of the quantum system, under the Markovian approximation. Assuming that initially the radiation field is prepared in a Glauber coherent state and the two-level atom is in the excited state, we investigate the influence of gravity on the temporal evolution of collapses and revivals of the atomic population inversion, atomic dipole squeezing, atomic momentum diffusion, photon counting statistics and quadrature squeezing of the radiation field in the presence of phase damping.Comment: 25 pages, 15 figure

Dynamics near the critical point: the hot renormalization group in quantum field theory

The perturbative approach to the description of long wavelength excitations at high temperature breaks down near the critical point of a second order phase transition. We study the \emph{dynamics} of these excitations in a relativistic scalar field theory at and near the critical point via a renormalization group approach at high temperature and an $\epsilon$ expansion in $d=5-\epsilon$ space-time dimensions. The long wavelength physics is determined by a non-trivial fixed point of the renormalization group. At the critical point we find that the dispersion relation and width of quasiparticles of momentum $p$ is $\omega_p \sim p^{z}$ and $\Gamma_p \sim (z-1) \omega_p$ respectively, the group velocity of quasiparticles $v_g \sim p^{z-1}$ vanishes in the long wavelength limit at the critical point. Away from the critical point for $T\gtrsim T_c$ we find $\omega_p \sim \xi^{-z}[1+(p \xi)^{2z}]^{{1/2}}$ and $\Gamma_p \sim (z-1) \omega_p \frac{(p \xi)^{2z}}{1+(p \xi)^{2z}}$ with $\xi$ the finite temperature correlation length $\xi \propto |T-T_c|^{-\nu}$. The new \emph{dynamical} exponent $z$ results from anisotropic renormalization in the spatial and time directions. For a theory with O(N) symmetry we find $z=1+ \epsilon \frac{N+2}{(N+8)^2}+\mathcal{O}(\epsilon^2)$. Critical slowing down, i.e, a vanishing width in the long-wavelength limit, and the validity of the quasiparticle picture emerge naturally from this analysis.Comment: Discussion on new dynamical universality class. To appear in Phys. Rev.

Partially Annealed Disorder and Collapse of Like-Charged Macroions

Charged systems with partially annealed charge disorder are investigated using field-theoretic and replica methods. Charge disorder is assumed to be confined to macroion surfaces surrounded by a cloud of mobile neutralizing counterions in an aqueous solvent. A general formalism is developed by assuming that the disorder is partially annealed (with purely annealed and purely quenched disorder included as special cases), i.e., we assume in general that the disorder undergoes a slow dynamics relative to fast-relaxing counterions making it possible thus to study the stationary-state properties of the system using methods similar to those available in equilibrium statistical mechanics. By focusing on the specific case of two planar surfaces of equal mean surface charge and disorder variance, it is shown that partial annealing of the quenched disorder leads to renormalization of the mean surface charge density and thus a reduction of the inter-plate repulsion on the mean-field or weak-coupling level. In the strong-coupling limit, charge disorder induces a long-range attraction resulting in a continuous disorder-driven collapse transition for the two surfaces as the disorder variance exceeds a threshold value. Disorder annealing further enhances the attraction and, in the limit of low screening, leads to a global attractive instability in the system.Comment: 21 pages, 2 figure

Superdeformed rotational bands in the Mercury region; A Cranked Skyrme-Hartree-Fock-Bogoliubov study

A study of rotational properties of the ground superdeformed bands in \Hg{0}, \Hg{2}, \Hg{4}, and \Pb{4} is presented. We use the cranked Hartree-Fock-Bogoliubov method with the {\skm} parametrization of the Skyrme force in the particle-hole channel and a seniority interaction in the pairing channel. An approximate particle number projection is performed by means of the Lipkin-Nogami prescription. We analyze the proton and neutron quasiparticle routhians in connection with the present information on about thirty presently observed superdeformed bands in nuclei close neighbours of \Hg{2}.Comment: 26 LaTeX pages, 14 uuencoded postscript figures included, Preprint IPN-TH 93-6

Nonlinear Integer Programming

Research efforts of the past fifty years have led to a development of linear integer programming as a mature discipline of mathematical optimization. Such a level of maturity has not been reached when one considers nonlinear systems subject to integrality requirements for the variables. This chapter is dedicated to this topic. The primary goal is a study of a simple version of general nonlinear integer problems, where all constraints are still linear. Our focus is on the computational complexity of the problem, which varies significantly with the type of nonlinear objective function in combination with the underlying combinatorial structure. Numerous boundary cases of complexity emerge, which sometimes surprisingly lead even to polynomial time algorithms. We also cover recent successful approaches for more general classes of problems. Though no positive theoretical efficiency results are available, nor are they likely to ever be available, these seem to be the currently most successful and interesting approaches for solving practical problems. It is our belief that the study of algorithms motivated by theoretical considerations and those motivated by our desire to solve practical instances should and do inform one another. So it is with this viewpoint that we present the subject, and it is in this direction that we hope to spark further research.Comment: 57 pages. To appear in: M. J\"unger, T. Liebling, D. Naddef, G. Nemhauser, W. Pulleyblank, G. Reinelt, G. Rinaldi, and L. Wolsey (eds.), 50 Years of Integer Programming 1958--2008: The Early Years and State-of-the-Art Surveys, Springer-Verlag, 2009, ISBN 354068274