Container seal for dusty environment

Method maintains cleanliness of joint-sealing surfaces under dust-laden conditions. This is accomplished by keeping seal and sealing surface covered with sliding plastic rings, which slide out of the way when a joint is seated

Optical network physical layer parameter optimization for digital backpropagation using Gaussian processes

We present a novel methodology for optimizing fiber optic network performance by determining the ideal values for attenuation, nonlinearity, and dispersion parameters in terms of achieved signal-to-noise ratio (SNR) gain from digital backpropagation (DBP). Our approach uses Gaussian process regression, a probabilistic machine learning technique, to create a computationally efficient model for mapping these parameters to the resulting SNR after applying DBP. We then use simplicial homology global optimization to find the parameter values that yield maximum SNR for the Gaussian process model within a set of a priori bounds. This approach optimizes the parameters in terms of the DBP gain at the receiver. We demonstrate the effectiveness of our method through simulation and experimental testing, achieving optimal estimates of the dispersion, nonlinearity, and attenuation parameters. Our approach also highlights the limitations of traditional one-at-a-time grid search methods and emphasizes the interpretability of the technique. This methodology has broad applications in engineering and can be used to optimize performance in various systems beyond optical networks

Efficacy of an 8-Week Concurrent Strength and Endurance Training Programme on Hand Cycling Performance

The aim of the present study was to investigate the effects of an 8-week concurrent strength and endurance training programme in comparison to endurance training only on several key determinants of hand cycling performance. Five H4 and five H3 classified hand cyclists with at least one year’s hand cycling training history consented to participate in the study. Subjects underwent a battery of tests to establish body mass, body composition, VO2peak, maximum aerobic power, gross mechanical efficiency, maximal upper body strength, and 30 km time trial performance. Subjects were matched into pairs based upon 30 km time trial performance and randomly allocated to either a concurrent strength and endurance or endurance training only, intervention group. Following an 8-week training programme based upon a conjugated block periodisation model, subjects completed a second battery of tests. A mixed model, 2-way analysis of variance (ANOVA) revealed no significant changes between groups. However, the calculation of effect sizes (ES) revealed that both groups demonstrated a positive improvement in most physiological and performance measures with subjects in the concurrent group demonstrating a greater magnitude of improvement in body composition (ES -0.80 vs. -0.22) maximal aerobic power (ES 0.97 vs. 0.28), gross mechanical efficiency (ES 0.87 vs. 0.63), bench press 1 repetition maximum (ES 0.53 vs. 0.33), seated row 1 repetition maximum (ES 1.42 vs. 0.43), and 30 km time trial performance (ES -0.66 vs. -0.30). In comparison to endurance training only, an 8-week concurrent training intervention based upon a conjugated block periodisation model appears to be a more effective training regime for improving the performance capabilities of hand cyclists

Stabilization of a-conotoxin AuIB: influences of disulfide connectivity and backbone cyclization

a-Conotoxins are peptides isolated from the venom ducts of cone snails that target nicotinic acetylcholine receptors (nAChRs). They are valuable pharmacological tools and have potential applications for treating a range of conditions in humans, including pain. However, like all peptides, conotoxins are susceptible to degradation, and to enhance their therapeutic potential it is important to elucidate the factors contributing to instability and to develop approaches for improving stability. AuIB is a unique member of the a-conotoxin family because the nonnative "ribbon" disulfide isomer exhibits enhanced activity at the nAChR in rat parasympathetic neurons compared with the native "globular" isomer. Here we show that the ribbon isomer of AuIB is also more resistant to disulfide scrambling, despite having a nonnative connectivity and flexible structure. This resistance to disulfide scrambling does not correlate with overall stability in serum because the ribbon isomer is degraded in human serum more rapidly than the globular isomer. Cyclization via the joining of the N- and C-termini with peptide linkers of four to seven amino acids prevented degradation of the ribbon isomer in serum and stabilized the globular isomers to disulfide scrambling. The linker length used for cyclization strongly affected the relative proportions of the disulfide isomers produced by oxidative folding. Overall, the results of this study provide important insights into factors influencing the stability and oxidative folding of a-conotoxin AuIB and might be valuable in the design of more stable antagonists of nAChRs

A declining major merger fraction with redshift in the local Universe from the largest-yet catalog of major and minor mergers in SDSS

It is difficult to accurately identify galaxy mergers and it is an even larger challenge to classify them by their mass ratio or merger stage. In previous work we used a suite of simulated mergers to create a classification technique that uses linear discriminant analysis (LDA) to identify major and minor mergers. Here, we apply this technique to 1.3 million galaxies from the SDSS DR16 photometric catalog and present the probability that each galaxy is a major or minor merger, splitting the classifications by merger stages (early, late, post-coalescence). We present publicly-available imaging predictor values and all of the above classifications for one of the largest-yet samples of galaxies. We measure the major and minor merger fraction ($f_{\mathrm{merg}}$) and build a mass-complete sample of galaxies, which we bin as a function of stellar mass and redshift. For the major mergers, we find a positive slope of $f_{\mathrm{merg}}$ with stellar mass and negative slope of $f_{\mathrm{merg}}$ with redshift between stellar masses of $10.5 < M_* (log\ M_{\odot}) < 11.6$ and redshifts of $0.03 < z < 0.19$. We are able to reproduce an artificial positive slope of the major merger fraction with redshift when we do not bin for mass or craft a complete sample, demonstrating the importance of mass completeness and mass binning. We determine that the positive trend of the major merger fraction with stellar mass is consistent with a hierarchical assembly scenario. The negative trend with redshift requires that an additional assembly mechanism, such as baryonic feedback, dominates in the local Universe.Comment: 29 pages, 22 figures, see figures 16 and 17 for a summary of the findings, accepted to MNRA

Expressing the Geobacter metallireducens PilA in Geobacter sulfurreducens Yields Pili with Exceptional Conductivity

ABSTRACT The electrically conductive pili (e-pili) of Geobacter sulfurreducens serve as a model for a novel strategy for long-range extracellular electron transfer. e-pili are also a new class of bioelectronic materials. However, the only other Geobacter pili previously studied, which were from G. uraniireducens , were poorly conductive. In order to obtain more information on the range of pili conductivities in Geobacter species, the pili of G. metallireducens were investigated. Heterologously expressing the PilA gene of G. metallireducens in G. sulfurreducens yielded a G. sulfurreducens strain, designated strain MP, that produced abundant pili. Strain MP exhibited phenotypes consistent with the presence of e-pili, such as high rates of Fe(III) oxide reduction and high current densities on graphite anodes. Individual pili prepared at physiologically relevant pH 7 had conductivities of 277 ± 18.9 S/cm (mean ± standard deviation), which is 5,000-fold higher than the conductivity of G. sulfurreducens pili at pH 7 and nearly 1 million-fold higher than the conductivity of G. uraniireducens pili at the same pH. A potential explanation for the higher conductivity of the G. metallireducens pili is their greater density of aromatic amino acids, which are known to be important components in electron transport along the length of the pilus. The G. metallireducens pili represent the most highly conductive pili found to date and suggest strategies for designing synthetic pili with even higher conductivities. IMPORTANCE e-pili are a remarkable electrically conductive material that can be sustainably produced without harsh chemical processes from renewable feedstocks and that contain no toxic components in the final product. Thus, e-pili offer an unprecedented potential for developing novel materials, electronic devices, and sensors for diverse applications with a new “green” technology. Increasing e-pili conductivity will even further expand their potential applications. A proven strategy is to design synthetic e-pili that contain tryptophan, an aromatic amino acid not found in previously studied e-pili. The studies reported here demonstrate that a productive alternative approach is to search more broadly in the microbial world. Surprisingly, even though G. metallireducens and G. sulfurreducens are closely related, the conductivities of their e-pili differ by more than 3 orders of magnitude. The ability to produce e-pili with high conductivity without generating a genetically modified product enhances the attractiveness of this novel electronic material

Prostate involvement during sexually transmitted infections as measured by prostate-specific antigen concentration

Background:We investigated prostate involvement during sexually transmitted infections by measuring serum prostate-specific antigen (PSA) as a marker of prostate infection, inflammation, and/or cell damage in young, male US military members.Methods:We measured PSA before and during infection for 299 chlamydia, 112 gonorrhoea, and 59 non-chlamydial, non-gonococcal urethritis (NCNGU) cases, and 256 controls.Results:Chlamydia and gonorrhoea, but not NCNGU, cases were more likely to have a large rise (⩾40%) in PSA than controls (33.6%, 19.1%, and 8.2% vs 8.8%, P<0.0001, 0.021, and 0.92, respectively).Conclusion:Chlamydia and gonorrhoea may infect the prostate of some infected men

The Close AGN Reference Survey (CARS): Locating the [O III] wing component in luminous local Type 1 AGN

Context. The strong asymmetry in the optical [O III] λ5007 emission line is one of the best signatures of active galactic nuclei (AGN) driven warm (∼104 K) ionized gas outflows on host galaxy scales. While large spectroscopic surveys such as the sloan digital sky survey (SDSS) have characterized the kinematics of [O III] for large samples of AGN, estimating the associated energetics requires spatially resolving these outflows with, for example, integral field unit (IFU) studies. Aims. As part of the Close AGN Reference Survey, we obtained spatially resolved IFU spectroscopy for a representative sample of 39 luminous type 1 AGN at 0.01 < z < 0.06 with the multi unit spectroscopic explorer and the visible multi object spectrograph IFUs at the very large telescope to infer the spatial location of the ionized gas outflows. Methods. We compared the 2D light distributions of the [O III] wing to that of the Hβ broad emission line region, a classical point source (PSF). We then used the PSF to distinguish between the unresolved and resolved [O III] wing emission. We further determined its location using spectro-astrometry for the point-like sources. Results. The [O III] wing is spatially unresolved in 23 out of the 36 AGN with > 80% of the flux associated with a point-like source. We measured < 100 pc offsets in the spatial location of the outflow from the AGN nucleus using the spectro-astrometry technique for these sources. For the other 13 AGN, the [O III] wing emission is resolved and possibly extended on several kiloparsec scales. Conclusions. We conclude that [O III] wing emission can be compact or extended in an unbiased luminous AGN sample, where both cases are likely to appear. Electron density in the compact [O III] wing regions (median ne ∼ 1900 cm−3) is nearly a magnitude higher than in the extended ones (median ne ∼ 500 cm−3). The presence of spatially extended and compact [O III] wing emission is unrelated to the AGN bolometric luminosity and to inclination effects, which means other features such as time delays, or mechanical feedback (radio jets) may shape the ionized gas outflow properties