4,514 research outputs found
Stem cells and fluid flow drive cyst formation in an invertebrate excretory organ.
Cystic kidney diseases (CKDs) affect millions of people worldwide. The defining pathological features are fluid-filled cysts developing from nephric tubules due to defective flow sensing, cell proliferation and differentiation. The underlying molecular mechanisms, however, remain poorly understood, and the derived excretory systems of established invertebrate models (Caenorhabditis elegans and Drosophila melanogaster) are unsuitable to model CKDs. Systematic structure/function comparisons revealed that the combination of ultrafiltration and flow-associated filtrate modification that is central to CKD etiology is remarkably conserved between the planarian excretory system and the vertebrate nephron. Consistently, both RNA-mediated genetic interference (RNAi) of planarian orthologues of human CKD genes and inhibition of tubule flow led to tubular cystogenesis that share many features with vertebrate CKDs, suggesting deep mechanistic conservation. Our results demonstrate a common evolutionary origin of animal excretory systems and establish planarians as a novel and experimentally accessible invertebrate model for the study of human kidney pathologies
Avionics architecture studies for the entry research vehicle
This report is the culmination of a year-long investigation of the avionics architecture for NASA's Entry Research Vehicle (ERV). The Entry Research Vehicle is conceived to be an unmanned, autonomous spacecraft to be deployed from the Shuttle. It will perform various aerodynamic and propulsive maneuvers in orbit and land at Edwards AFB after a 5 to 10 hour mission. The design and analysis of the vehicle's avionics architecture are detailed here. The architecture consists of a central triply redundant ultra-reliable fault tolerant processor attached to three replicated and distributed MIL-STD-1553 buses for input and output. The reliability analysis is detailed here. The architecture was found to be sufficiently reliable for the ERV mission plan
A feasibility study of signed consent for the collection of patient identifiable information for a national paediatric clinical audit database
Objectives: To investigate the feasibility of obtaining signed consent
for submission of patient identifiable data to a national clinical
audit database and to identify factors influencing the consent process
and its success.
Design: Feasibility study.
Setting: Seven paediatric intensive care units in England.
Participants: Parents/guardians of patients, or patients aged 12-16
years old, approached consecutively over three months for signed
consent for submission of patient identifiable data to the national
clinical audit database the Paediatric Intensive Care Audit Network
(PICANet).
Main outcome measures: The numbers and proportions of admissions for
which signed consent was given, refused, or not obtained (form not
returned or form partially completed but not signed), by age, sex,
level of deprivation, ethnicity (South Asian or not), paediatric index
of mortality score, length of hospital stay (days in paediatric
intensive care).
Results: One unit did not start and one did not fully implement the
protocol, so analysis excluded these two units. Consent was obtained
for 182 of 422 admissions (43%) (range by unit 9% to 84%). Most
(101/182; 55%) consents were taken by staff nurses. One refusal (0.2%)
was received. Consent rates were significantly better for children who
were more severely ill on admission and for hospital stays of six days
or more, and significantly poorer for children aged 10-14 years. Long
hospital stays and children aged 10-14 years remained significant in a
stepwise regression model of the factors that were significant in the
univariate model.
Conclusion: Systematically obtaining individual signed consent for
sharing patient identifiable information with an externally located
clinical audit database is difficult. Obtaining such consent is
unlikely to be successful unless additional resources are specifically
allocated to training, staff time, and administrative support
Robustness of the Blandford-Znajek mechanism
The Blandford-Znajek mechanism has long been regarded as a key ingredient in
models attempting to explain powerful jets in AGNs, quasars, blazzars etc. In
such mechanism, energy is extracted from a rotating black hole and dissipated
at a load at far distances. In the current work we examine the behaviour of the
BZ mechanism with respect to different boundary conditions, revealing the
mechanism robustness upon variation of these conditions. Consequently, this
work closes a gap in our understanding of this important scenario.Comment: 7 pages, accepted in CQ
Magnetic Reconnection with Radiative Cooling. I. Optically-Thin Regime
Magnetic reconnection, a fundamental plasma process associated with a rapid
dissipation of magnetic energy, is believed to power many disruptive phenomena
in laboratory plasma devices, the Earth magnetosphere, and the solar corona.
Traditional reconnection research, geared towards these rather tenuous
environments, has justifiably ignored the effects of radiation on the
reconnection process. However, in many reconnecting systems in high-energy
astrophysics (e.g., accretion-disk coronae, relativistic jets, magnetar flares)
and, potentially, in powerful laser plasma and z-pinch experiments, the energy
density is so high that radiation, in particular radiative cooling, may start
to play an important role. This observation motivates the development of a
theory of high-energy-density radiative magnetic reconnection. As a first step
towards this goal, we present in this paper a simple Sweet--Parker-like theory
of non-relativistic resistive-MHD reconnection with strong radiative cooling.
First, we show how, in the absence of a guide magnetic field, intense cooling
leads to a strong compression of the plasma in the reconnection layer,
resulting in a higher reconnection rate. The compression ratio and the layer
temperature are determined by the balance between ohmic heating and radiative
cooling. The lower temperature in the radiatively-cooled layer leads to a
higher Spitzer resistivity and hence to an extra enhancement of the
reconnection rate. We then apply our general theory to several specific
astrophysically important radiative processes (bremsstrahlung, cyclotron, and
inverse-Compton) in the optically thin regime, for both the zero- and
strong-guide-field cases. We derive specific expressions for key reconnection
parameters, including the reconnection rate. We also discuss the limitations
and conditions for applicability of our theory.Comment: 31 pages, 1 figur
Using Mussel Isotope Ratios to Assess Anthropogenic Nitrogen Inputs to Freshwater Ecosystems
Stable nitrogen isotope ratios (δ15N) of freshwater mussels from a series of lakes and ponds were related to watershed land use characteristics to assess their utility in determining the source of nitrogen inputs to inland water bodies. Nitrogen isotope ratios measured in freshwater mussels from 19 lakes and ponds in Rhode Island, U.S.A., ranged from 4.9–12.6% and were found to significantly correlate with the fraction of residential development in 100 and 200 m buffer zones around the ponds. Mussel δ15N values in 12 of the 19 ponds also showed significant correlation with average dissolved nitrate concentrations, which ranged from 23–327 μg L-1. These observations, in light of previous studies which link elevated δ15N values of nitrogen derived from septic wastewater with those seen in biota, suggest that mussel isotope ratios may reflect nitrogen source in freshwater ecosystems. We followed an iterative approach using multiple regression analysis to assess the relationship between mussel δ15N and the land use categories fraction residential development, fraction feedlot agriculture, fraction row-crop agriculture, and fraction natural vegetation in 100 and 200 m buffer zones and pond watersheds. From this we developed a simple regression model to predict mussel δ15N from the fraction of residential development in the 200 m buffer zone around the pond. Subsequent testing with data from 16 additional sites in the same ecoregion led us to refine the model by incorporating the fraction of natural vegetation. The overall average absolute difference between measured and predicted δ15N values using the two-parameter model was 1.6%. Potential sources of error in the model include differences in the scale and categorization of land-use data used to generate and test the model, differences in physical characteristics, such as retention time and range of residential development, and exclusion of sources of enriched nitrogen such as runoff from feedlot operations or increased nitrogen loading from inefficient or failed septic systems
A PC parallel port button box provides millisecond response time accuracy under Linux
For psychologists, it is sometimes necessary to measure people's reaction times to the nearest millisecond. This article describes how to use the PC parallel port to receive signals from a button box to achieve millisecond response time accuracy. The workings of the parallel port, the corresponding port addresses, and a simple Linux program for controlling the port are described. A test of the speed and reliability of button box signal detection is reported. If the reader is moderately familiar with Linux, this article should provide sufficient instruction for him or her to build and test his or her own parallel port button box. This article also describes how the parallel port could be used to control an external apparatus
Accuracy of magnetic resonance imaging for measuring maturing cartilage: A phantom study
OBJECTIVES: To evaluate the accuracy of magnetic resonance imaging measurements of cartilage tissue-mimicking phantoms and to determine a combination of magnetic resonance imaging parameters to optimize accuracy while minimizing scan time. METHOD: Edge dimensions from 4 rectangular agar phantoms ranging from 10.5 to 14.5 mm in length and 1.25 to 5.5 mm in width were independently measured by two readers using a steel ruler. Coronal T1 spin echo (T1 SE), fast spoiled gradient-recalled echo (FSPGR) and multiplanar gradient-recalled echo (GRE MPGR) sequences were used to obtain phantom images on a 1.5-T scanner. RESULTS: Inter- and intra-reader reliability were high for both direct measurements and for magnetic resonance imaging measurements of phantoms. Statistically significant differences were noted between the mean direct measurements and the mean magnetic resonance imaging measurements for phantom 1 when using a GRE MPGR sequence (512x512 pixels, 1.5-mm slice thickness, 5:49 min scan time), while borderline differences were noted for T1 SE sequences with the following parameters: 320x320 pixels, 1.5-mm slice thickness, 6:11 min scan time; 320x320 pixels, 4-mm slice thickness, 6:11 min scan time; and 512x512 pixels, 1.5-mm slice thickness, 9:48 min scan time. Borderline differences were also noted when using a FSPGR sequence with 512x512 pixels, a 1.5-mm slice thickness and a 3:36 min scan time. CONCLUSIONS: FSPGR sequences, regardless of the magnetic resonance imaging parameter combination used, provided accurate measurements. The GRE MPGR sequence using 512x512 pixels, a 1.5-mm slice thickness and a 5:49 min scan time and, to a lesser degree, all tested T1 SE sequences produced suboptimal accuracy when measuring the widest phantom
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