Continental-shelf-scale model of the Leeuwin Current

A simple model is presented for the poleward eastern boundary current (the Leeuwin Current) off Western Australia. For continental-shelf length-scales and seasonal time-scales, the advective and time-derivative terms are small, and water flows onto the shelf until a sufficient cross-shelf pressure gradient is set up to push the same flux back. In a rotating system, the return flux takes place in a frictional (Ekman) layer at the bottom, and is synonymous with a near-bottom longshore current νB, from−CD|νB|νB = ∫O−H 1/ρ ∂p/∂y dz + u2∗,which is equatorward close to shore, but poleward past the 40 m isobath. If the mixed layer is deep enough, there is no upwelling, despite the upwelling-favorable winds. The light surface water is pushed down, causing a baroclinic shear enhancing the poleward current. Advection causes an intense sloping density, salinity, and tracers front. Observed u2∗ and Py from Western Australia predict νB to be poleward in early winter at about 0.2 m s−l, and near zero in summer. The sea-level-slope gηy correlates highly (r = 0.9) with the wind-stress u2∗ with a regression of (100 m)−1, both along Western Australia and western North America

Primary progressive multiple sclerosis presenting under the age of 18 years: fact or fiction?

Previous cohort studies on pediatric multiple sclerosis (MS) have reported very low frequencies for a primary progressive MS (PPMS) course ranging from 0 to 7%. We identified six patients presenting prior to the age of 18 years and fulfilling the 2017 McDonald Criteria for primary progressive multiple sclerosis (PPMS). Presentation with progressive neurological symptoms and signs in young people should prompt evaluation for genetic causes such as leukodystrophies, hereditary spastic paraparesis and mitochondrial diseases given the rarity of primary progressive course in pediatric MS. In the absence of an alternative diagnosis, with new therapeutic options becoming available for PPMS, this diagnosis should then be considered

Dissipation in Poynting-flux Dominated Flows: the Sigma-Problem of the Crab Pulsar Wind

Flows in which energy is transported predominantly as Poynting flux are thought to occur in pulsars, gamma-ray bursts and relativistic jets from compact objects. The fluctuating component of the magnetic field in such a flow can in principle be dissipated by magnetic reconnection, and used to accelerate the flow. We investigate how rapidly this transition can take place, by implementing into a global MHD model, that uses a thermodynamic description of the plasma, explicit, physically motivated prescriptions for the dissipation rate: a lower limit on this rate is given by limiting the maximum drift speed of the current carriers to that of light, an upper limit follows from demanding that the dissipation zone expand only subsonically in the comoving frame and a further prescription is obtained by assuming that the expansion speed is limited by the growth rate of the relativistic tearing mode. In each case, solutions are presented which give the Lorentz factor of a spherical wind containing a transverse, oscillating magnetic field component as a function of radius. In the case of the Crab pulsar, we find that the Poynting flux can be dissipated before the wind reaches the inner edge of the Nebula if the pulsar emits electron positron pairs at a rate >1.E40 per second, thus providing a possible solution to the sigma-problem.Comment: Accepted for publication in Ap

Ribosomal protein synthesis is not regulated at the translational level in Saccharomyces cerevisiae: balanced accumulation of ribosomal proteins L16 and rp59 is mediated by turnover of excess protein.

We have investigated the mechanisms whereby equimolar quantities of ribosomal proteins accumulate and assemble into ribosomes of the yeast Saccharomyces cerevisiae. Extra copies of the cry1 or RPL16 genes encoding ribosomal proteins rp59 or L16 were introduced into yeast by transformation. Excess cry1 or RPL16 mRNA accumulated in polyribosomes in these cells and was translated at wild-type rates into rp59 or L16 proteins. These excess proteins were degraded until their levels reached those of other ribosomal proteins. Identical results were obtained when the transcription of RPL16A was rapidly induced using GAL1-RPL16A promoter fusions, including a construct in which the entire RPL16A 5\u27-noncoding region was replaced with the GAL1 leader sequence. Our results indicate that posttranscriptional expression of the cry1 and RPL16 genes is regulated by turnover of excess proteins rather than autogenous regulation of mRNA splicing or translation. The turnover of excess rp59 or L16 is not affected directly by mutations that inactivate vacuolar hydrolases

Low Energy Electron Point Projection Microscopy of Suspended Graphene, the Ultimate "Microscope Slide"

Point Projection Microscopy (PPM) is used to image suspended graphene using low-energy electrons (100-200eV). Because of the low energies used, the graphene is neither damaged or contaminated by the electron beam. The transparency of graphene is measured to be 74%, equivalent to electron transmission through a sheet as thick as twice the covalent radius of sp^2-bonded carbon. Also observed is rippling in the structure of the suspended graphene, with a wavelength of approximately 26 nm. The interference of the electron beam due to the diffraction off the edge of a graphene knife edge is observed and used to calculate a virtual source size of 4.7 +/- 0.6 Angstroms for the electron emitter. It is demonstrated that graphene can be used as both anode and substrate in PPM in order to avoid distortions due to strong field gradients around nano-scale objects. Graphene can be used to image objects suspended on the sheet using PPM, and in the future, electron holography

Using tasks to explore teacher knowledge in situation-specific contexts

This article was published in the journal, Journal of Mathematics Teacher Education [© Springer] and the original publication is available at www.springerlink.comResearch often reports an overt discrepancy between theoretically/out-of context expressed teacher beliefs about mathematics and pedagogy and actual practice. In order to explore teacher knowledge in situation-specific contexts we have engaged mathematics teachers with classroom scenarios (Tasks) which: are hypothetical but grounded on learning and teaching issues that previous research and experience have highlighted as seminal; are likely to occur in actual practice; have purpose and utility; and, can be used both in (pre- and in-service) teacher education and research through generating access to teachers’ views and intended practices. The Tasks have the following structure: reflecting upon the learning objectives within a mathematical problem (and solving it); examining a flawed (fictional) student solution; and, describing, in writing, feedback to the student. Here we draw on the written responses to one Task (which involved reflecting on solutions of x+x−1=0 of 53 Greek in-service mathematics teachers in order to demonstrate the range of teacher knowledge (mathematical, didactical and pedagogical) that engagement with these tasks allows us to explore

Microscopic analysis of shot-noise suppression in nondegenerate diffusive conductors

We present a theoretical investigation of shot-noise suppression due to long-range Coulomb interaction in nondegenerate diffusive conductors. Calculations make use of an ensemble Monte Carlo simulator self-consistently coupled with a one-dimensional Poisson solver. We analyze the noise in a lightly doped active region surrounded by two contacts acting as thermal reservoirs. By taking the doping of the injecting contacts and the applied voltage as variable parameters, the influence of elastic and inelastic scattering in the active region is investigated. The transition from ballistic to diffusive transport regimes under different contact injecting statistics is analyzed and discussed. Provided significant space-charge effects take place inside the active region, long-range Coulomb interaction is found to play an essential role in suppressing the shot noise at $qU \gg k_BT$. In the elastic diffusive regime, momentum space dimensionality is found to modify the suppression factor $\gamma$, which within numerical uncertainty takes values respectively of about 1/3, 1/2 and 0.7 in the 3D, 2D and 1D cases. In the inelastic diffusive regime, shot noise is suppressed to the thermal value.Comment: 11 pages, 13 figure

Kinetic Characterisation of a Single Chain Antibody against the Hormone Abscisic Acid: Comparison with Its Parental Monoclonal

A single-chain Fv fragment antibody (scFv) specific for the plant hormone abscisic acid (ABA) has been expressed in the bacterium Escherichia coli as a fusion protein. The kinetics of ABA binding have been measured using surface plasmon resonance spectrometry (BIAcore 2000) using surface and solution assays. Care was taken to calculate the concentration of active protein in each sample using initial rate measurements under conditions of partial mass transport limitation. The fusion product, parental monoclonal antibody and the free scFv all have low nanomolar affinity constants, but there is a lower dissociation rate constant for the parental monoclonal resulting in a three-fold greater affinity. Analogue specificity was tested and structure-activity binding preferences measured. The biologically-active (+)-ABA enantiomer is recognised with an affinity three orders of magnitude higher than the inactive (-)-ABA. Metabolites of ABA including phaseic acid, dihydrophaseic acid and deoxy-ABA have affinities over 100-fold lower than that for (+)-ABA. These properties of the scFv make it suitable as a sensor domain in bioreporters specific for the naturally occurring form of ABA