Dichotomy In Response To Indomethacin In Uv-C And Uv-B Induced Ultraviolet Light Inflammation

In subjects irradiated with both UV-C and UV-B ultraviolet light (UVL), 10 μg of intradermal indomethacin decreased the redness in all 13 of the UV-B irradiated areas but in only 2 of 13 of the UV-C irradiated areas. Higher doses of intradermal indomethacin (50 μg and 100 μg) decreased the redness produced by UV-C irradiation in 6 subjects. It is suggested that the failure of 10 μg of indomethacin to decrease the redness of the UV-C induced inflammation, while decreasing the redness in the UV-R induced inflammation, is consistent with the possibility that prostaglandins participate in UV-R but not UV-C induced inflammation

the influence of pregnancy and the beginning of lactation on pelage traits in cashmere goats

The aim of this study was to investigate the effect of pregnancy followed by the beginning of lactation on fibre traits incashmere goats. Two groups of cashmere-bearing goats aged between 2-3 years were used. The control group (A) included12 non-lactating, non-pregnant subjects. The experimental group (B) included 12 goats mated during the first weekof June, in order to have pregnancy and the beginning of lactation coincide with the period when cashmere normallygrows. As expected, Liveweight significantly varied in Group B during the last two months of pregnancy, when foetalgrowth reaches its maximum, and following delivery. Hair patch weight, because of the continuous growth of primary andsecondary fibres, increased significantly during the trial (P<0.001). The physiological status considered negatively affected(P<0.05) the total mean growth rate of cashmere fibres and, as a result, their length. Cashmere daily growth ratevalues varied significantly (P<0.05) throughout the experiment, while the effect of the physiological status was notedonly in November – December. Furthermore, this parameter also seems to be influenced by climatic factors and, in particular,environmental temperature, as shown by the negative correlation (r = - 0.28; P<0.05) between cashmere dailygrowth rate and environmental temperature. Guard hair length and growth rate did not differ between the two groups,however, they were influenced by time. Cashmere yield and cashmere production were lower in group B (P<0.05). Nodifferences between groups were observed for cashmere diameter. Overall, pregnancy and the consequent period of lactationnegatively influenced cashmere rather than guard hair fibres. These negative effects were noted in quantitativeterms as yield and production dropped by 37% and 43%, respectively. We hypothesise that the complete overlap of pregnancyand lactation with the period of cashmere growth reduced the number of secondary active follicles and their degreeof activity and caused an increase in competition for the partitioning of nutrients between hair follicles and the graviduterus, first, and then the mammary gland, later

Epithelium-Off Corneal Collagen Cross-linking Versus Transepithelial Cross-linking for Pediatric Keratoconus.

Abstract PURPOSE:: To compare efficiency and safety of epithelium-off corneal cross-linking (CXL) and transepithelial cross-linking (TE-CXL) in pediatric patients with progressive keratoconus. METHODS:: Uncorrected and corrected visual acuity, corneal topography and pachymetry (Pentacam; Oculus Pentacam), and in vivo confocal microscopy (HRT II, Rostock Cornea Module, Heidelberg Engineering, Heidelberg, Germany) were evaluated at baseline and at 3, 6, and 12 months. RESULTS:: In the epithelium-off CXL group (19 patients, 23 eyes; mean age, 14.75 ± 2.1 years), a significant improvement at month 12 was present for Kmax [-1.11 diopters (D), P = 0.01], Kmin (-3.2 D, P = 0.001), mean K (-1.47 D, P = 0.01), surface asymmetry index (-0.64 D, P = 0.001), inferior-superior symmetry index (-0.54 D, P = 0.01), index of height asymmetry (-2.97, P = 0.03), and anterior elevation at the thinnest location (-2.82 D, P = 0.01) and at the apex (-2.27 D, P = 0.01). Postoperative corneal edema lasted 3 months in 16 eyes (69.5%) and more than 6 months in 2 eyes (8.7%). In the TE-CXL group (10 patients, 14 eyes; mean age, 15 ± 4.2 years), a significant improvement at month 12 was present for Kmax (-1.14 D, P = 0.02), Kmin (-2.04 D, P = 0.01), mean K (-1.63 D, P = 0.01), surface asymmetry index (-0.86 D, P = 0.001), inferior-superior symmetry index (-0.55 D, P = 0.001), index of height asymmetry (-2.95, P = 0.01), and anterior elevation at the thinnest location (-2.96 D, P = 0.01) and at the apex (-2.19 D, P = 0.01). No postoperative corneal edema after TE-CXL was observed. Changes at month 12 from baseline were not significantly different between the 2 groups (P > 0.05). TE-CXE was significantly less painful than epithelium-off CXL. CONCLUSIONS:: In pediatric patients with progressive keratoconus, TE-CXL was less painful, provided similar effectiveness and fewer complications than epithelium-off CXL at 12-month follow-up

Un nuevo anestésico intravenoso: propofol : evaluación clínica y experimental en el perro

El propofol es un anestésico intravenoso no relacionado químicamente con ningún otro anestésico conocido. La inducción anestésica con propofol es rápida y el mantenimiento puede realizarse, bien mediante infusión continua, bien con dosis de mantenimiento en «bolus», y no presenta efecto acumulativo como ocurre con el tiopental sódico, anestésico muy similar en cuanto a acción anestésica y que sirve de referencia. Puede complementarse con analgésicos opiáceos u óxido nitroso para proporcionar analgesia. Al ser la recuperación anestésica más rápida en comparación con otros agentes anestésicos intravenosos o intramusculares, se reduce la aparición de complicaciones.Propofol is a new intravenous anaesthetic agent chemically unrelated to any other anaesthetic. When propofol is used, anaesthetic induction is fast and maintenance can be achieved by continuous infusion or by incremental doses in «bolus», without the cumulative effect seen with thiopentone, a well known barbiturate with similar anaesthetic activity and used as a reference drug. Complementation with opioids or nitrous oxide can provide adequate analgesia. Compared to other intravenous or intramuscular anaesthetic agents, anaesthetic recovery is fast, reducing the complications associated with more prolonged recoveries

A worm gel-based 3D model to elucidate the paracrine interaction between multiple myeloma and mesenchymal stem cells

Multiple myeloma (MM) is a malignancy of terminally-differentiated plasma cells that develops mainly inside the bone marrow (BM) microenvironment. It is well known that autocrine and paracrine signals are responsible for the progression of this disease but the precise mechanism and contributions from single cell remain largely unknown. Mesenchymal stem cells (MSC) are an important cellular component of the BM: they support MM growth by increasing its survival and chemo-resistance, but little is known about the paracrine signaling pathways. Three-dimensional (3D) models of MM-MSC paracrine interactions are much more biologically-relevant than simple 2D models and are considered essential for detailed studies of MM pathogenesis. Herein we present a novel 3D co-culture model designed to mimic the paracrine interaction between MSC and MM cells. MSC were embedded within a previously characterized thermoresponsive block copolymer worm gel that can induce stasis in human pluripotent stem cells (hPSC) and then co-cultured with MM cells. Transcriptional phenotyping of co-cultured cells indicated the dysregulation of genes that code for known disease-relevant factors, and also revealed IL-6 and IL-10 as upstream regulators. Importantly, we have identified a synergistic paracrine signaling pathway between IL-6 and IL-10 that plays a critical role in sustaining MM cell proliferation. Our findings indicate that this 3D co-culture system is a useful model to investigate the paracrine interaction between MM cells and the BM microenvironment in vitro. This approach has revealed a new mechanism that promotes the proliferation of MM cells and suggested a new therapeutic target

Collections Education: The Extended Specimen and Data Acumen

Biodiversity scientists must be fluent across disciplines; they must possess the quantitative, computational, and data skills necessary for working with large, complex data sets, and they must have foundational skills and content knowledge from ecology, evolution, taxonomy, and systematics. To effectively train the emerging workforce, we must teach science as we conduct science and embrace emerging concepts of data acumen alongside the knowledge, tools, and techniques foundational to organismal biology. We present an open education resource that updates the traditional plant collection exercise to incorporate best practices in twenty-first century collecting and to contextualize the activities that build data acumen. Students exposed to this resource gained skills and content knowledge in plant taxonomy and systematics, as well as a nuanced understanding of collections-based data resources. We discuss the importance of the extended specimen in fostering scientific discovery and reinforcing foundational concepts in biodiversity science, taxonomy, and systematics

Discrete-row growth of xenon adsorbed on the vicinal Pt(997) surface: Comparison between theory and experiment

Xe exhibits a discrete-row growth mode on the vicinal Pt(997) surface by sequential attachment to the substrate steps. In order to interpret experimental results obtained by grazing incidence helium scattering, potential calculations are performed. A mean-field Hamiltonian within the two-dimensional Ising model is shown to explain the sequential-row growth observed in helium-atom diffraction studies. More specifically, the calculated temperatures for the occurrence of each row depend mainly on the shape of the potential increment due to the steps and countersteps. They are in good agreement with the experimental values associated with maxima in the scattered He intensity versus coverage curves

Omalizumab decreases exacerbation frequency, oral intake of corticosteroids and peripheral blood eosinophils in atopic patients with uncontrolled asthma.

Omalizumab is a humanized monoclonal anti-IgE antibody approved in 2005 by the European Medicine Agency (EMA) for the treatment of severe persistent allergic asthma, which remains inadequately controlled despite optimal therapy with high doses of inhaled corticosteroids and long-acting β2-adrenergic agonists. Within this context, the present observational study refers to 16 patients currently treated with omalizumab at the Respiratory Unit of "Magna Græcia" University Hospital located in Catanzaro, Italy, whose anti- IgE therapy was started in the period included between March 2007 and February 2010, thus lasting at least 10 months. After 40 weeks of add-on treatment with omalizumab, very relevant decreases were detected, in comparison with pre-treatment mean (± standard deviation) values, in monthly exacerbation numbers (from 1.1 ± 0.6 to 0.2 ± 0.4; p < 0.01) and oral corticosteroid consumption (from 22.6 ± 5.0 to 1.2 ± 2.9 mg/day of prednisone; p < 0.01). These changes were associated with stable improvements in lung function, expressed as increases of both FEV1 (from 53.6 ± 14.6% to 77.0 ± 14.9% of predicted values; p < 0.01) and FEV1/FVC ratio (from 56.3 ± 9.5% to 65.8 ± 9.2%; p < 0.01). Moreover, in 5 patients who persistently had increased numbers of eosinophils (mean ± SD: 15.9 ± 8.0% of total WBC count; absolute number: 1,588.0 ± 956.9/μl) despite a long-lasting therapy with inhaled and systemic corticosteroids, the peripheral counts of these cells decreased down to near normal levels (mean ± SD: 6.3 ± 2.3% of total WBC count; absolute number: 462.0 ± 262.3/μl) after 16 weeks of treatment with omalizumab. Therefore, this descriptive evaluation confirms the efficacy of add-on omalizumab therapy in selected patients with exacerbation-prone, chronic allergic uncontrolled asthma, requiring a continuous intake of oral corticosteroids

Color stabilization of apulian red wines through the sequential inoculation of starmerella bacillaris and saccharomyces cerevisiae

Mixed fermentation using Starmerella bacillaris and Saccharomyces cerevisiae has gained attention in recent years due to their ability to modulate the qualitative parameters of enological interest, such as the color intensity and stability of wine. In this study, three of the most important red Apulian varieties were fermented through two pure inoculations of Saccharomyces cerevisiae strains or the sequential inoculation of Saccharomyces cerevisiae after 48 h from Starmerella bacillaris. The evolution of anthocyanin profiles and chromatic characteristics were determined in the produced wines at draining off and after 18 months of bottle aging in order to assess the impact of the different fermentation protocols on the potential color stabilization and shelf-life. The chemical composition analysis showed titratable acidity and ethanol content exhibiting marked differences among wines after fermentation and aging. The 48 h inoculation delay produced wines with higher values of color intensity and color stability. This was ascribed to the increased presence of compounds, such as stable A-type vitisins and reddish/violet ethylidene-bridge flavonol-anthocyanin adducts, in the mixed fermentation. Our results proved that the sequential fermentation of Starmerella bacillaris and Saccharomyces cerevisiae could enhance the chromatic profile as well as the stability of the red wines, thus improving their organoleptic quality