The optimization of force inputs for active structural acoustic control using a neural network

This paper investigates the use of a neural network to determine which force actuators, of a multi-actuator array, are best activated in order to achieve structural-acoustic control. The concept is demonstrated using a cylinder/cavity model on which the control forces, produced by piezoelectric actuators, are applied with the objective of reducing the interior noise. A two-layer neural network is employed and the back propagation solution is compared with the results calculated by a conventional, least-squares optimization analysis. The ability of the neural network to accurately and efficiently control actuator activation for interior noise reduction is demonstrated

A stability study and moral analysis of a space-station - Centrifuge configuration

Dynamic stability of space station-centrifuge configuratio

Mobile radio alternative systems study, executive summary

Present day mobile communication technologies, systems and equipment are described from background in evaluating the concepts generated in the study. Average propagation ranges are calculated for terrestrial installations in each of seven physiographic areas of the contiguous states to determine the number of installations that would be required for nationwide coverage. Four system concepts are defined and analyzed to determine how well terrestrial systems can fulfill the requirements at acceptable costs

An evaluation of active noise control in a cylindrical shell

The physical mechanisms governing the use of active noise control in an extended volume of a cylindrical shell are discussed. Measured data was compared with computer results from a previously derived analytical model based on an infinite shell theory. For both the analytical model and experiment, the radiation of the external monopoles is coupled to the internal acoustic field through the radial displacement of the thin, elastic cylindrical shell. An active noise control system was implemented in the cylinder using a fixed array of discrete monopole sources, all of which lie in the plane of the exterior noise sources. Good agreement between measurement and prediction was obtained for both internal pressure response and overall noise reduction. Attenuations in the source plane greater than 15 dB were recorded along with a uniformly quieted noise environment over the entire length of the experimental model. Results indicate that for extended axial forcing distributions or very low shell damping, axial arrays of control sources may be required. Finally, the Nyquist criteria for the number of azimuthal control sources is shown to provide for effective control over the full cylinder cross section

Limb darkening in spherical stellar atmospheres

(Abridged) Context. Stellar limb darkening, I({\mu} = cos{\theta}), is an important constraint for microlensing, eclipsing binary, planetary transit, and interferometric observations, but is generally treated as a parameterized curve, such as a linear-plus-square-root law. Many analyses assume limb-darkening coefficients computed from model stellar atmospheres. However, previous studies, using I({\mu}) from plane- parallel models, have found that fits to the flux-normalized curves pass through a fixed point, a common {\mu} location on the stellar disk, for all values of T eff, log g and wavelength. Aims. We study this fixed {\mu}-point to determine if it is a property of the model stellar atmospheres or a property of the limb-darkening laws. Furthermore, we use this limb-darkening law as a tool to probe properties of stellar atmospheres for comparison to limb- darkening observations. Methods. Intensities computed with plane-parallel and spherically-symmetric Atlas models (characterized by the three fundamental parameters L\star, M\star and R\star) are used to reexamine the existence of the fixed {\mu}-point for the parametrized curves. Results. We find that the intensities from our spherical models do not have a fixed point, although the curves do have a minimum spread at a {\mu}-value similar to the parametrized curves. We also find that the parametrized curves have two fixed points, {\mu}1 and {\mu}2, although {\mu}2 is so close to the edge of the disk that it is missed using plane-parallel atmospheres. We also find that the spherically- symmetric models appear to agree better with published microlensing observations relative to plane-parallel models.Comment: 8 pages, 8 figures, figures 4 and 6 have lower resolution. A&A in pres

Using limb darkening to measure fundamental parameters of stars

Context. Limb darkening is an important tool for understanding stellar atmospheres, but most observations measuring limb darkening assume various parameterizations that yield no significant information about the structure of stellar atmospheres. Aims. We use a specific limb-darkening relation to study how the best-fit coefficients relate to fundamental stellar parameters from spherically symmetric model stellar atmospheres. Methods. Using a grid of spherically symmetric Atlas model atmospheres, we compute limb-darkening coefficients, and develop a novel method to predict fundamental stellar parameters. Results. We find our proposed method predicts the mass of stellar atmosphere models given only the radius and limb-darkening coefficients, suggesting that microlensing, interferometric, transit and eclipse observations can constrain stellar masses. Conclusions. This novel method demonstrates that limb-darkening parameterizations contain important information about the structure of stellar atmospheres, with the potential to be a valuable tool for measuring stellar masses.Comment: 8 pages, 6 figures, 2 tables, A&A accepte

Voltage-dependent Block of the Cystic Fibrosis Transmembrane Conductance Regulator Cl- Channel by Two Closely Related Arylaminobenzoates

The gene defective in cystic fibrosis encodes a Cl- channel, the cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is blocked by diphenylamine-2-carboxylate (DPC) when applied extracellularly at millimolar concentrations. We studied the block of CFTR expressed in Xenopus oocytes by DPC or by a closely related molecule, flufenamic acid (FFA). Block of whole-cell CFTR currents by bath-applied DPC or by FFA, both at 200 µM, requires several minutes to reach full effect. Blockade is voltage dependent, suggesting open-channel block: currents at positive potentials are not affected but currents at negative potentials are reduced. The binding site for both drugs senses ~40% of the electric field across the membrane, measured from the inside. In single-channel recordings from excised patches without blockers, the conductance was 8.0 ± 0.4 pS in symmetric 150 mM Cl^-. A subconductance state, measuring ~60% of the main conductance, was often observed. Bursts to the full open state lasting up to tens of seconds were uninterrupted at depolarizing membrane voltages. At hyperpolarizing voltages, bursts were interrupted by brief closures. Either DPC or FFA (50 µM) applied to the cytoplasmic or extracellular face of the channel led to an increase in flicker at V_m =-100 mV and not at V_m = +100 mV, in agreement with whole-cell experiments. DPC induced a higher frequency of flickers from the cytoplasmic side than the extracellular side. FFA produced longer closures than DPC; the FFA closed time was roughly equal (~ 1.2 ms) at -100 mV with application from either side. In cell-attached patch recordings with DPC or FFA applied to the bath, there was flickery block at V_m = -100 mV, confirming that the drugs permeate through the membrane to reach the binding site. The data are consistent with the presence of a single binding site for both drugs, reached from either end of the channel. Open-channel block by DPC or FFA may offer tools for use with site-directed mutagenesis to describe the permeation pathway

The alternate GNB3 splice variant, Gβ3s, exhibits an altered signalling response to EGF stimulation, which leads to enhanced cell migration

It has recently been reported that the duplication of the GNB3 gene has been shown to be directly linked to an obesity phenotype, both in humans and also in a humanised mouse model. Moreover, the common human GNB3 c.825C>T polymorphism (rs5443) causes this ubiquitously expressed gene to be aberrantly spliced approximately 50% of the time leading to the production of both a normal Gβ3 protein and a truncated, possibly less stable subunit, known as Gβ3s. The presence of the GNB3 825T allele has previously been shown to be associated with predisposition to hypertension, obesity, various cancers, Alzheimers, age related cognitive function, erectile dysfunction as well as a marker for pharmacogenetic drug action. Great controversy, however, currently exists as to whether these phenotypes associated with the 825T allele are a) mainly due to the presence of the smaller, possibly more active, Gβ3s subunit or b) merely down to the haploinsufficiency of the normal GNB3 transcript, due to its frequent aberrant splicing. In order to try and address these two conflicting hypothesis, we report on the identification and characterisation of signalling alterations unique to the presence of Gβ3s protein subunit. Moreover we also show the physiological consequences associated with altered signalling, directly induced by the Gβ3s subunit. For this, we used both an EBV transformed lymphoblast cell line homozygote for GNB3 825T/825T (TT) and a stable Gβ3s expressing recombinant COS-7 clone. In both of these cell lines that express the Gβ3s subunit, we found enhanced cytosolic calcium influx upon stimulation with EGF, TGFα and VEGF ligands, as compared to “normal” GNB3 controls with the 825C/825C (CC) genotype. This aberrant calcium influx also led to an increase in ERK, but not AKT1, phosphorylation. Despite the lack of AKT1 activation, we paradoxically observed a significant increase in phosphorylation of its downstream substrates, namely mTOR and p70S6k (KS6B2). Moreover we observed a decrease in phospho FoxO3a only in Gβ3s expressing cells, but not in the “normal” GNB3 (CC) control cell line. The presence of the Gβ3s subunit also appeared to alter the distinct localisation patterns of both Foxo3a and AKT1, while also increasing the colocalisation of mTOR and p70S6K. Subsequent growth factor stimulation studies revealed that EGF treatment, of Gβ3s expressing cells, appeared to cause a significant decrease in cAMP levels, which, in turn resulted in both enhanced caveolin-1a phosphorylation, and an increase in actin stress fibre formation. The identification of these distinct Gβ3s specific signalling alterations were indicative of a more aggressive migratory phenotype. This led us to further investigate and confirm that the presence of the Gβ3s subunit also appears to cause significantly enhanced migration and robust scratch wound healing kinetics, as compared to cells harbouring only the normal copy of the gene. These data therefore present convincing evidence that the Gβ3s subunit is stable, functional and its presence can significantly alter signalling pathways, in different cell types

A far-infrared study of N/O abundance ratio in galactic H 2 regions

Far-infrared lines of N++ and O++ in several galactic H II regions were measured in an effort to probe the abundance ratio N/O. New measurements are presented for W32 (630.8-0.0), Orion A, and G75.84+0.4. The combination of (N III) 57.3 millimicrons and (O III) 88.4 and 51.8 millimicrons yields measurements of N++/O++ that are largely insensitive to electron temperature, density uncertainties, and to clumping of the ionized gas, due to the similarity of the critical densities for these transitions. In the observed nebulae, N++/O++ should be indicative of N/O, a ratio that is of special importance in nucleosynthesis theory. Measurements are compared with previous measurements of M17 and W51. For nebulae in the solar circle, N++/O++ is greater than the N/O values derived from optical studies of N+/O+ in low ionization zones of the same nebulae. We find that N++/O++ in W43 is significantly higher than for the other H II regions in the sample. Since W43 is located at R = 5 kpc, which is the smallest galactocentric distance in our sample, our data appear consistent with the presence of a negative abundance gradient d(N/O)dR

Rodent Aβ Modulates the Solubility and Distribution of Amyloid Deposits in Transgenic Mice

The amino acid sequence of amyloid precursor protein (APP) is highly conserved, and age-related Abeta aggregates have been described in a variety of vertebrate animals, with the notable exception of mice and rats. Three amino acid substitutions distinguish mouse and human Abeta that might contribute to their differing properties in vivo. To examine the amyloidogenic potential of mouse Abeta, we studied several lines of transgenic mice overexpressing wild-type mouse amyloid precursor protein (moAPP) either alone or in conjunction with mutant PS1 (PS1dE9). Neither overexpression of moAPP alone nor co-expression with PS1dE9 caused mice to develop Alzheimer-type amyloid pathology by 24 months of age. We further tested whether mouse Abeta could accelerate the deposition of human Abeta by crossing the moAPP transgenic mice to a bigenic line expressing human APPswe with PS1dE9. The triple transgenic animals (moAPP x APPswe/PS1dE9) produced 20% more Abeta but formed amyloid deposits no faster and to no greater extent than APPswe/PS1dE9 siblings. Instead, the additional mouse Abeta increased the detergent solubility of accumulated amyloid and exacerbated amyloid deposition in the vasculature. These findings suggest that, although mouse Abeta does not influence the rate of amyloid formation, the incorporation of Abeta peptides with differing sequences alters the solubility and localization of the resulting aggregates