A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm

The SR proteins constitute a large family of nuclear phosphoproteins required for constitutive pre-mRNA splicing. These factors also have global, concentration-dependent effects on alternative splicing regulation and this activity is antagonized by members of the hnRNP A/B family of proteins. We show here that whereas some human SR proteins are confined to the nucleus, three of them-SF2/ASF, SRp20, and 9G8-shuttle rapidly and continuously between the nucleus and the cytoplasm. By swapping the corresponding domains between shuttling and nonshuttling SR proteins, we show that the carboxy-terminal arginine/serine-rich (RS) domain is required for shuttling. This domain, however, is not sufficient to promote shuttling of an unrelated protein reporter, suggesting that stable RNA binding mediated by the RNA-recognition motifs may be required for shuttling. Consistent with such a requirement, a double point-mutation in RRM1 of SF2/ASF that impairs RNA binding prevents the protein from shuttling. In addition, we show that phosphorylation of the RS domain affects the shuttling properties of SR proteins. These findings show that different SR proteins have unique intracellular transport properties and suggest that the family members that shuttle may have roles not only in nuclear pre-mRNA splicing but also in mRNA transport, cytoplasmic events, and/or processes that involve communication between the nucleus and the cytoplasm

Urban Planning and Natural Hazard Governance

The establishment of effective linkages between institutional urban planning and disaster risk strategies remains a challenge for formal governance structures. For governments at all administrative scales, disaster resilience planning has required systemic capacities that rely on structures of governance, humanitarian frameworks, and budgetary capacities. However, with growing urbanization trends, humanitarian responses and Disaster Risk Management (DRM) frameworks have had to adapt their operations in contexts with high population density, complex infrastructure systems, informal dynamics, and a broader range of actors. Urban areas concentrate an array of different groups with the capability of contributing to urban responses and strategies to cope with disaster effects, including community groups, government agencies, international organizations and humanitarian practitioners. In addition, cities have running planning structures that support their administration and spatial organization, with instruments that supply constant information about population characteristics, infrastructure capacity and potential weaknesses. Processes and data ascribed to urban planning can provide vital knowledge to natural hazard governance frameworks, from technical resources to conceptual approaches towards spatial analysis. Authorities managing risk could improve their strategic objectives if they could access and integrate urban planning information. Furthermore, a collaborative hazard governance can provide equity to multiple urban actors that are usually left out of institutional DRM, including nongovernmental organizations, academia, and community groups. Traditional top-down models can operate in parallel with horizontal arrangements, giving voice to groups with limited access to political platforms but who are knowledgeable on urban space and social codes. Their still limited recognition is evidence that there is still a disconnect between the intentions of global frameworks for inclusive governance, and the co-production of an urban planning designed for inclusive resilience

Anelastic Phenomena in Mg-Al Alloys

Cyclic loading-unloading in tension and compression has been used to quantify the anelastic behaviour, in the form of hysteresis loops, of pure Mg and three Mg-Al alloys (0.5, 2, and 9 at.% Al). The effect reached a maximum at a plastic strain of approximate to 0.02 for all of the materials, and decreased at higher strains. The amount of anelasticity at any given strain was smaller for the dilute alloys in comparison with the pure Mg whereas it increased above that of pure Mg for the most concentrated alloy. Possible reasons for this behaviour are discussed in terms of reversible twinning, solid solution softening, and hardening and short range order

Ecogeographical Variation in Skull Shape of South-American Canids: Abiotic or Biotic Processes?

Species morphological changes can be mutually influenced by environmental or biotic factors, such as competition. South American canids represent a quite recent radiation of taxa that evolved forms very disparate in phenotype, ecology and behaviour. Today, in the central part of South America there is one dominant large species (the maned wolf, Chrysocyon brachyurus) that directly influence sympatric smaller taxa via interspecific killing. Further south, three species of similar sized foxes (Lycalopex spp.) share the same habitats. Such unique combination of taxa and geographic distribution makes South American dogs an ideal group to test for the simultaneous impact of climate and competition on phenotypic variation. Using geometric morphometrics, we quantified skull size and shape of 431 specimens belonging to the eight extant South American canid species: Atelocynus microtis, Cerdocyon thous, Ch. brachyurus, Lycalopex culpaeus, L. griseus, L. gymnocercus, L. vetulus and Speothos venaticus. South American canids are significantly different in both skull size and shape. The hypercarnivorous bush dog is mostly distinct in shape from all the other taxa while a degree of overlap in shape—but not size—occurs between species of the genus Lycalopex. Both climate and competition impacts interspecific morphological variation. We identified climatic adaptations as the main driving force of diversification for the South American canids. Competition has a lower degree of impact on their skull morphology although it might have played a role in the past, when canid community was richer in morphotypes

New Supergravity Backgrounds Dual to N=1 SQCD-like Theories with N_f=2N_c

We present new supergravity backgrounds generated by N_c D5-branes, wrapping the S^2 of the resolved conifold, in the presence of N_f = 2 N_c smeared flavor D5-branes. The smearing allows us to take their backreaction on the geometry into account. We discuss the consistency, stability, and supersymmetry of these types of setups. We find near horizon geometries that we expect to be supergravity duals of SQCD-like theories with N_f= 2N_c. From these backgrounds we numerically extract rectangular Wilson loops and beta functions of the dual field theory for the regime where our approximations are valid.Comment: 22+24 pages, 17 figures, Figure 12 replace

Vortexing Off A Common Suction Header

This project is the first iteration of a testing rig to determine the critical submergence of a vortex off a main suction header. The rig was designed with supplies purchased and provided by PG&E, including 4-6” schedule 40 PVC pipe and fittings, a large water tank, a pump, and a flowmeter. PG&E at Diablo Canyon Power Plant presented their problem to the Cal Poly Mechanical Engineering Senior Project class, and three mechanical engineers took up the project. The following report details the ideation, design, build, and test processes used during the 2016-17 academic year to create the vortex testing rig. We determined through testing that the location of hydraulic jump can be influenced by how open or closed the branch line valve was, which in turn influenced when gas ingestion to the pump occurred

An upper limit on the mass of the circumplanetary disk for DH Tau b

DH Tau is a young ($\sim$1 Myr) classical T Tauri star. It is one of the few young PMS stars known to be associated with a planetary mass companion, DH Tau b, orbiting at large separation and detected by direct imaging. DH Tau b is thought to be accreting based on copious H${\alpha}$ emission and exhibits variable Paschen Beta emission. NOEMA observations at 230 GHz allow us to place constraints on the disk dust mass for both DH Tau b and the primary in a regime where the disks will appear optically thin. We estimate a disk dust mass for the primary, DH Tau A of $17.2\pm1.7\,M_{\oplus}$, which gives a disk-to-star mass ratio of 0.014 (assuming the usual Gas-to-Dust mass ratio of 100 in the disk). We find a conservative disk dust mass upper limit of 0.42$M_{\oplus}$ for DH Tau b, assuming that the disk temperature is dominated by irradiation from DH Tau b itself. Given the environment of the circumplanetary disk, variable illumination from the primary or the equilibrium temperature of the surrounding cloud would lead to even lower disk mass estimates. A MCFOST radiative transfer model including heating of the circumplanetary disk by DH Tau b and DH Tau A suggests that a mass averaged disk temperature of 22 K is more realistic, resulting in a dust disk mass upper limit of 0.09$M_{\oplus}$ for DH Tau b. We place DH Tau b in context with similar objects and discuss the consequences for planet formation models.Comment: accepted for publication in A

Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors

The SR proteins are essential metazoan pre-mRNA splicing factors that can also influence the selection of alternative 5' splice sites in a concentration-dependent manner. Their activity in alternative splicing in vitro is antagonized by members of the hnRNP A/B family of proteins. The opposite effects of members of these two families of antagonistic splicing factors in vitro and upon overexpression in vivo suggest that changes in their relative levels may be a natural mechanism for the regulation of alternative splicing in vivo. One prediction of this model is that the ratios of these antagonists should vary in different cell types and in other situations in which cellular or viral transcripts are differentially spliced. We raised monoclonal antibodies specific for SFS/ASF and used them to measure the abundance of SFS/ASF protein and its isoforms, its phosphorylation state in vivo and during splicing in vitro, and its association with the spliceosome. SF2/ASF exists predominantly or exclusively in a highly phosphorylated state in vivo in all cell types examined, and unphosphorylated protein was not detectable. Unphosphorylated recombinant SFS/ASF becomes rapidly phosphorylated under splicing conditions in HeLa cell extracts and associates stably with one or more exons of beta-globin pre-mRNA. This interaction appears to persist through the splicing reaction and SF2/ASF remains bound to spliced mRNA. We compared the distribution of SFS/ASF to that of its antagonist, hnRNP Al, in different rat tissues and in immortal and transformed cell lines. We found that the protein levels of these antagonistic splicing factors vary naturally over a very wide range, supporting the notion that changes in the ratio of these proteins can affect alternative splicing of a variety of pre-mRNAs in vivo

The inner environment of Z~CMa: High-Contrast Imaging Polarimetry with NaCo

Context. Z\,CMa is a binary composed of an embedded Herbig Be and an FU Ori class star separated by $\sim100$ au. Observational evidence indicate a complex environment in which each star has a circumstellar disk and drives a jet, and the whole system is embedded in a large dusty envelope. Aims. We aim to probe the circumbinary environment of Z\,CMa in the inner 400 au in scattered light. Methods. We use high contrast imaging polarimetry with VLT/NaCo at $H$ and $K_s$ bands. Results. The central binary is resolved in both bands. The polarized images show three bright and complex structures: a common dust envelope, a sharp extended feature previously reported in direct light, and an intriguing bright clump located 0\farcs3 south of the binary, which appears spatially connected to the sharp extended feature. Conclusions.We detect orbital motion when compared to previous observations, and report a new outburst driven by the Herbig star. Our observations reveal the complex inner environment of Z\,CMa with unprecedented detail and contrast.Comment: Accepted for publication in A&A Letter

Solvent and stabilizer free growth of Ag and Pd nanoparticles using metallic salts/cyclotriphosphazenes mixtures

Cyclotriphosphazene is used as a sacrificial solid-state template to synthesize a range of Ag and Pd nanoparticles with diverse geometries by thermal treatment using MLn/N3P3(O2C12H8)3 mixtures. The Pd and Ag nanoparticles are synthesized by solid-state pyrolysis of AgPPh3[CF3SO3]/N3P3(O2C12H8)3 and PdCl2/N3P3(O2C12H8)3 mixtures with molar relationships of 1:1, 1:5 and 1:10 respectively, in air and at 800 °C. The morphology of the as-prepared nanoparticles is found to depend on the molar ratio of the precursor mixture, the preparation method and of the nature of the metal. Ag and Pd, microcrystals were thermally grown on Si from the respective 1:1 precursors while that metal foams were grown from 1:5 ratios precursors on SiO2 wafers. High resolution transmission electron microscopy investigations reveal in most cases small crystals of Pd. HRSTEM measurements indicate that the formation of the Pd and Ag nanoparticles occurs through a phase demixing and dewetting mechanism. This approach has potential to be a useful and facile method to prepare metallic nanoparticles without requiring solutions or surfactants for application in electronic, catalytic and sensor materials and devices