Transcriptional responses of winter barley to cold indicate nucleosome remodelling as a specific feature of crown tissues

We report a series of microarray-based comparisons of gene expression in the leaf and crown of the winter barley cultivar Luxor, following the exposure of young plants to various periods of low (above and below zero) temperatures. A transcriptomic analysis identified genes which were either expressed in both the leaf and crown, or specifically in one or the other. Among the former were genes responsible for calcium and abscisic acid signalling, polyamine synthesis, late embryogenesis abundant proteins and dehydrins. In the crown, the key organ for cereal overwintering, cold treatment induced transient changes in the transcription of nucleosome assembly genes, and especially H2A and HTA11, which have been implicated in cold sensing in Arabidopsis thaliana. In the leaf, various heat-shock proteins were induced. Differences in expression pattern between the crown and leaf were frequent for genes involved in certain pathways responsible for osmolyte production (sucrose and starch, raffinose, γ-aminobutyric acid metabolism), sugar signalling (trehalose metabolism) and secondary metabolism (lignin synthesis). The action of proteins with antifreeze activity, which were markedly induced during hardening, was demonstrated by a depression in the ice nucleation temperature

Green HRM promotes higher education sustainability:a mediated-moderated analysis

Abstract Purpose: In the contemporary world, where sustainability at higher education is at the forefront, the ever-changing business ecosystem nurtures a new drift towards economic, environmental and social performance. This study aims to measure the impact of green human resource management (GHRM) practices on sustainability in the higher education of a developing country context through a theoretical aspect of Resource-Based View (RBV). Design/methodology/approach: This study adopted a quantitative approach to propose and test a model based on predictors of sustainability. The survey approach received 190 responses from employees (faculty and non-faculty members) working as a full-time in the 40 higher education institutions (HEIs) of Pakistan. The structural and measurement model was calculated using SmartPLS. Findings: The results show that “green training and development” (GTD) and “top management commitment towards greening workforce (TMCGW)” have a positive relationship with sustainability while green recruitment and selection (GRS) was not supported by sustainability. The mediating effect of TMCGW plays a crucial role between GTD and sustainability. Also, this study contributes through the moderating interaction effect of Gender between GTD and sustainability. Overall the GHRM practices promote employee green behavior and sustainability. Originality/value: The proposed research model in the current study is a substantial gap in the literature and exploring this connection requires new theoretical frameworks. To bridge this literature gap, this study examined the role of GHRM on sustainability at micro-level (employee perspective) through a theoretical aspect of RBV in the developing country higher education context of Pakistan. Importantly, this study enhances the understanding of the emerging global wave of green mobility and highlights the impact of GHRM practices on sustainability through perception of academic professionals

Allium genetic resources

An overview of the developments in Allium genetic resources during the past 25 years is presented in this chapter. A first important development has been the introduction and further development of web-based genebanking information systems (e.g. GENESYS, PLANTSEARCH), which facilitated the exchange of data to a large extent between Allium collection holders worldwide. These information systems made it possible to obtain an overview of the Allium genetic resources managed worldwide and identify the gaps in collections which still need to be filled, especially in the face of the ongoing genetic erosion. A second important area of progress has been the development of new methods for the maintenance of Allium germplasm, especially cryopreservation. This method has made it possible to maintain Allium accessions in a cheap and effective way. The method is especially important for the conservation of vegetatively maintained germplasm. Other developments in Allium genebanking are the improvement of the health status of the germplasm kept in the collections and the continuing characterization and evaluation of germplasm, which stimulates the utilization of the Allium genetic resources held in genebanks. Significant changes could also be observed with respect to acquisition and exchange of plant genetic resources due to many and complex new regulations on the legal and organizational levels due to the adoption of the CBD and IT-PGRFA by many countries. It makes the handling of the plant accessions safer and more consistent but also more circumstantial. Finally, we need to underline that in an increasingly changing world with all the threats of genetic erosion and extinction due to disappearance of traditional cultivation methods, devastation of our environment and climatic change, the conservation of genetic resources is of prime importance for agriculture. Especially for breeders, a highly diverse genepool of a crop plant is an invaluable treasure. The importance to keep this treasure will no doubt become even more important in the future

The up-regulation of elongation factors in the barley leaf and the down-regulation of nucleosome assembly genes in the crown are both associated with the expression of frost tolerance

We report a series of microarray-based leaf and crown transcriptome comparisons involving three barley cultivars (cvs. Luxor, Igri and Atlas 68) which express differing degrees of frost tolerance. The transcripts were obtained following the exposure of seedlings to low (above and below zero) temperatures, aiming to identify those genes and signalling/metabolic pathways which are associated with frost tolerance. Both the leaves and the crowns responded to low temperature by the up-regulation of a suite of abscisic acid (ABA)-responsive genes, most of which have already been recognized as components of the plant low temperature response. The inter-cultivar comparison indicated that genes involved in maintaining the leaf's capacity to synthesize protein and to retain chloroplast activity were important for the expression of frost tolerance. In the crown, the repression of genes associated with nucleosome assembly and transposon regulation were the most relevant transcriptional changes associated with frost tolerance, highlighting the role of gene repression in the cold acclimation response