Efeito de indutores de resistência na incidência do mal do Panamá em banana maçã.

O mal do Panamá ou murcha de Fusarium, causado por Fusarium oxysporum Schlechtend.: Fr. f.sp. cubense (E.F. Smith) W.C. Snyder & Hansen, é uma das doenças mais importantes da bananeira (Musa spp.). Primeiramente relatado na Austrália, esta doença está presente em todas as regiões produtoras do mundo. Nas décadas de 1950 e 1960, o mal do Panamá dizimou a bananicultura da América Central e Caribe, então constituída unicamente da variedade Gros Michel (Daly & Walduck, 2006). No Brasil, onde foi detectado em 1930 sobre banana Maçã (Cordeiro et al., 2003), essa doença causa perdas acentuadas na produção de frutos em todo o País (Cordeiro et al., 1993), notadamente na variedade Maçã e naquelas do subgrupo Prata.pdf 202

Potencial de uso de rizobactérias para o controle da fusariose do abacaxizeiro.

Atualmente, o Brasil é o maior produtor mundial de abacaxi com uma produção de 2,52 milhões de toneladas. Dentre as regiões produtoras, o Nordeste brasileiro é responsável por aproximadamente 46% desta produção. Na Bahia, quarto produtor nacional, a abacaxicultura é praticada em diversas microrregiões, a exemplo do município de Itaberaba localizado no semi-árido baiano. A abacaxicultura exerce importante papel socioeconômico onde é praticada, uma vez que contribui para geração de emprego e renda, além da fixação do homem no campo. Entretanto, a fusariose, doença causada por Fusarium guttiforme (sin: F. subglutinans), provoca perdas significativas constituindo o principal fator limitante da cultura. O controle da doença baseiase na integração de práticas culturais, incluindo a aplicação de fungicidas. Consciente dos problemas ambientais promovidos pelo uso de agroquímicos, a sociedade tem exigido métodos alternativos ao uso destes produtos. Dentre as alternativas, o controle biológico vem sendo intensivamente estudado como ferramenta no controle integrado de diversas doenças de plantas. Todavia, estudos neste sentido referentes ao patossistema abacaxi - F. guttiforme são escassos ou nulos. Neste estudo objetivou-se verificar o potencial de uso de rizobactérias para o controle da fusariose do abacaxizeiro.PDF. 057

GFP and RFP transformation of Fusarium guttiforme (sin. Fusarium subglutinans f. sp. annanas).

Edição dos Resumos do 42° Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009

Induction of autophagy is a key component of all-trans-retinoic acid-induced differentiation in leukemia cells and a potential target for pharmacological modulation

Acute myeloid leukemia (AML) is characterized by the accumulation of immature blood cell precursors in the bone marrow. Pharmacologically overcoming the differentiation block in this condition is an attractive therapeutic avenue, which has achieved success only in a subtype of AML, acute promyelocytic leukemia (APL). Attempts to emulate this success in other AML subtypes have thus far been unsuccessful. Autophagy is a conserved protein degradation pathway with important roles in mammalian cell differentiation, particularly within the hematopoietic system. In the study described here, we investigated the functional importance of autophagy in APL cell differentiation. We found that autophagy is increased during all-trans-retinoic acid (ATRA)-induced granulocytic differentiation of the APL cell line NB4 and that this is associated with increased expression of LC3II and GATE-16 proteins involved in autophagosome formation. Autophagy inhibition, using either drugs (chloroquine/3-methyladenine) or short-hairpin RNA targeting the essential autophagy gene ATG7, attenuates myeloid differentiation. Importantly, we found that enhancing autophagy promotes ATRA-induced granulocytic differentiation of an ATRA-resistant derivative of the non-APL AML HL60 cell line (HL60-Diff-R). These data support the development of strategies to stimulate autophagy as a novel approach to promote differentiation in AML

Sialic Acid Glycobiology Unveils Trypanosoma cruzi Trypomastigote Membrane Physiology.

Trypanosoma cruzi, the flagellate protozoan agent of Chagas disease or American trypanosomiasis, is unable to synthesize sialic acids de novo. Mucins and trans-sialidase (TS) are substrate and enzyme, respectively, of the glycobiological system that scavenges sialic acid from the host in a crucial interplay for T. cruzi life cycle. The acquisition of the sialyl residue allows the parasite to avoid lysis by serum factors and to interact with the host cell. A major drawback to studying the sialylation kinetics and turnover of the trypomastigote glycoconjugates is the difficulty to identify and follow the recently acquired sialyl residues. To tackle this issue, we followed an unnatural sugar approach as bioorthogonal chemical reporters, where the use of azidosialyl residues allowed identifying the acquired sugar. Advanced microscopy techniques, together with biochemical methods, were used to study the trypomastigote membrane from its glycobiological perspective. Main sialyl acceptors were identified as mucins by biochemical procedures and protein markers. Together with determining their shedding and turnover rates, we also report that several membrane proteins, including TS and its substrates, both glycosylphosphatidylinositol-anchored proteins, are separately distributed on parasite surface and contained in different and highly stable membrane microdomains. Notably, labeling for α(1,3)Galactosyl residues only partially colocalize with sialylated mucins, indicating that two species of glycosylated mucins do exist, which are segregated at the parasite surface. Moreover, sialylated mucins were included in lipid-raft-domains, whereas TS molecules are not. The location of the surface-anchored TS resulted too far off as to be capable to sialylate mucins, a role played by the shed TS instead. Phosphatidylinositol-phospholipase-C activity is actually not present in trypomastigotes. Therefore, shedding of TS occurs via microvesicles instead of as a fully soluble form

Integration of ground measurements to model- derived data : A report of IEA SHC Task 46 Solar Resource Assessment and Forecasting

Bankable data for solar energy projects needs to ensure as much as possible the accuracy and general quality of solar radiation data to be used in the solar resource assessment studies for any site of interest in a project development. The term “site adaptation” is being used in the framework of solar energy projects to refer to the improvement that can be achieved in satellite-derived (or more generally model-derived) solar irradiance when short-term local ground measurements are used to correct systematic errors and bias of the original dataset. This document presents a review of different techniques for correcting long-term satellite- derived solar radiation data by using short-term ground measurements. The collaborative work has been done within the framework of Task 46 “Solar Resource Assessment and Forecasting” of the International Energy Agency's Solar Heating and Cooling Programme. Different approaches whose use depends on the origin and characteristics of the uncertainties of the modelled data are presented. Recommendations to the use of ground measurements and the results of several approaches to improve satellite-derived data are shown through this report highlighting the importance of using site adaptation and the different degree of improvement that can be achieved depending on the climatological characteristics of the site

Multidisciplinary nutritional consensus on assessment and nutritional dietary treatment in patients with chronic kidney disease and SARS-CoV-2 infection

The presence of malnutrition in patients with Chronic Kidney Disease (CKD) is high, it can be made worse by SARS-CoV2 infection. The nutritional assessment should be adapted to minimize the infection, recommending monitoring: weight loss percentage, body mass index (BMI), loss of appetite, analytical parameters and functional capacity using the dynamometer. As well as the sarcopenia assessment using the SCARF scale, and the possibility of using the GLIM criteria in those patients who have been tested positive by MUST. It is important to adapt the nutritional recommendations in the caloric and protein intake, to the CKD stage and to the SARS-CoV2 infection stage. In patients with hypercatabolism, to prioritize preserving the nutritional status (35 kcal/kg weight/day, proteins up to 1.5 g/kg/day). The rest of the nutrients will be adapted to CKD stage and the analytical values. In the post-infection stage, a complete nutritional assessment is recommended, including sarcopenia. The energy and protein requirements in this phase will be adapted to the nutritional status, with special attention to the loss of muscle mass. Dietary recommendations need to be tailored to side effects of SARS-CoV-2 infection: anorexia, dysphagia, dysgeusia, and diarrhea. Anorexia and hypercatabolism makes it difficult to meet the requirements through diet, therefore the use of oral nutritional supplements is recommended as well as the enteral or parenteral nutrition in severe phases. La presencia de malnutrición en pacientes con Enfermedad Renal Crónica (ERC) es elevada, puede agravarse por la infección por SARS-CoV2. La valoración nutricional se debe adaptar para minimizar contagios, recomendando monitorizar: porcentaje de pérdida de peso, índice de masa corporal (IMC), pérdida de apetito, parámetros analíticos y capacidad funcional mediante dinamometría. Así como valorar la sarcopenia mediante la escala SCARF, y la posibilidad de utilizar los criterios GLIM en aquellos pacientes que el cribado MUST ha dado positivo. Es importante adaptar las recomendaciones nutricionales en ingesta calórica y proteica, al estadio de la ERC y a la fase de infección por SARS-CoV2. En pacientes hipercatabólicos priorizar preservar estado nutricional (35 kcal/kg peso/día, proteínas hasta 1,5 g/kg/día). El resto de nutrientes se adaptarán a estadío de ERC y valores analíticos. En la etapa post-infección, se recomienda realizar valoración nutricional completa, incluyendo sarcopenia. Los requerimientos energéticos y proteicos en esta fase se adaptarán a la afectación del estado nutricional, con especial atención a la pérdida de masa muscular. Es necesario adaptar las recomendaciones dietéticas a efectos secundarios de la infección por SARS-CoV-2: anorexia, disfagia, disgeusia, y diarrea. La anorexia y el hipercatabolismo dificulta el cumplimiento de los requerimiento a través de la alimentación, por lo que se recomienda la utilización de soporte nutricional oral y en las fases severas la nutrición enteral o la parenteral