1,710 research outputs found
High spatial resolution and high contrast optical speckle imaging with FASTCAM at the ORM
In this paper, we present an original observational approach, which combines,
for the first time, traditional speckle imaging with image post-processing to
obtain in the optical domain diffraction-limited images with high contrast
(1e-5) within 0.5 to 2 arcseconds around a bright star. The post-processing
step is based on wavelet filtering an has analogy with edge enhancement and
high-pass filtering. Our I-band on-sky results with the 2.5-m Nordic Telescope
(NOT) and the lucky imaging instrument FASTCAM show that we are able to detect
L-type brown dwarf companions around a solar-type star with a contrast DI~12 at
2" and with no use of any coronographic capability, which greatly simplifies
the instrumental and hardware approach. This object has been detected from the
ground in J and H bands so far only with AO-assisted 8-10 m class telescopes
(Gemini, Keck), although more recently detected with small-class telescopes in
the K band. Discussing the advantage and disadvantage of the optical regime for
the detection of faint intrinsic fluxes close to bright stars, we develop some
perspectives for other fields, including the study of dense cores in globular
clusters. To the best of our knowledge this is the first time that high
contrast considerations are included in optical speckle imaging approach.Comment: Proceedings of SPIE conference - Ground-based and Airborne
Instrumentation for Astronomy III (Conference 7735), San Diego 201
CHK1 expression in gastric cancer is modulated by p53 and RB1/E2F1: Implications in chemo/radiotherapy response
Radiation has a limited but relevant role in the adjuvant therapy of gastric cancer (GC) patients. Since Chk1 plays a critical function in cellular response to genotoxic agents, we aimed to analyze the role of Chk1 in GC as a biomarker for radiotherapy resistance. We analyzed Chk1 expression in AGS and MKN45 human GC cell lines by RT-QPCR and WB and in a small cohort of human patient’s samples. We demonstrated that Chk1 overexpression specifically increases resistance to radiation in GC cells. Accordingly, abrogation of Chk1 activity with UCN-01 and its expression with shChk1 increased sensitivity to bleomycin and radiation. Furthermore, when we assessed Chk1 expression in human samples, we found a correlation between nuclear Chk1 accumulation and a decrease in progression free survival. Moreover, using a luciferase assay we found that Chk1’s expression is controlled by p53 and RB/E2F1 at the transcriptional level. Additionally, we present preliminary data suggesting a posttranscriptional regulation mechanism, involving miR-195 and miR-503, which are inversely correlated with expression of Chk1 in radioresistant cells. In conclusion, Chk1/microRNA axis is involved in resistance to radiation in GC, and suggests Chk1 as a potential tool for optimal stratification of patients susceptible to receive adjuvant radiotherapy after surgeryThis work was supported by Instituto de Salud Carlos III–Fondo de Investigación Sanitaria (PS09/1988 to ISP; PI11-00949, pI014-1495 and Feder Funds to RP); Comunidad Autónoma de Madrid-Universidad Autónoma de Madrid (CCG10-UAM/BIO-5871 to ISP); Fundación Leticia Castillejo Castillo and Ministerio de Ciencia e Innovación (SAF2012-30862 to RSP), Spain
Potentiation of radiation therapy by the oncolytic adenovirus dl1520 (ONYX-015) in human malignant glioma xenografts
In spite of aggressive surgery, irradiation and/or chemotherapy, treatment of malignant gliomas remains a major challenge in adults and children due to high treatment failure. We have demonstrated significant cell lysis and antitumour activity of the E1B-55 kDa-gene-deleted adenovirus ONYX-015 (dl1520, CI-1042; ONYX Pharmaceuticals) in subcutaneous human malignant glioma xenografts deriving from primary tumours. Here, we show the combined efficacy of this oncolytic therapy with radiation therapy. Total body irradiation (5 Gy) of athymic nude mice prior to intratumoral injections of ONYX-015 1 x 10(8) PFU daily for 5 consecutive days yielded additive tumour growth delays in the p53 mutant xenograft IGRG88. Radiation therapy was potentiated in the p53 functional tumour IGRG121 with a 'subtherapeutic' dose of 1 x 10(7) PFU daily for 5 consecutive days, inducing significant tumour growth delay, 90% tumour regression and 50% tumour-free survivors 4 months after treatment. These potentiating effects were not due to increased adenoviral infectivity or replication. Furthermore, cell lysis and induction of apoptosis, the major mechanisms for adenoviral antitumour activity, did not play a major role in the combined treatment strategy. Interestingly, the oncolytic adenovirus seemed to accelerate radiation-induced tumour fibrosis. Potentiating antitumour activity suggests the development of this combined treatment for these highly malignant tumours
Commutative -algebras of Toeplitz operators on complex projective spaces
We prove the existence of commutative -algebras of Toeplitz operators on
every weighted Bergman space over the complex projective space
. The symbols that define our algebras are those that
depend only on the radial part of the homogeneous coordinates. The algebras
presented have an associated pair of Lagrangian foliations with distinguished
geometric properties and are closely related to the geometry of
High contrast optical imaging of companions: the case of the brown dwarf binary HD-130948BC
High contrast imaging at optical wavelengths is limited by the modest
correction of conventional near-IR optimized AO systems.We take advantage of
new fast and low-readout-noise detectors to explore the potential of fast
imaging coupled to post-processing techniques to detect faint companions to
stars at small separations. We have focused on I-band direct imaging of the
previously detected brown dwarf binary HD130948BC,attempting to spatially
resolve the L2+L2 benchmark system. We used the Lucky-Imaging instrument
FastCam at the 2.5-m Nordic Telescope to obtain quasi diffraction-limited
images of HD130948 with ~0.1" resolution.In order to improve the detectability
of the faint binary in the vicinity of a bright (I=5.19 \pm 0.03) solar-type
star,we implemented a post-processing technique based on wavelet transform
filtering of the image which allows us to strongly enhance the presence of
point-like sources in regions where the primary halo dominates. We detect for
the first time the BD binary HD130948BC in the optical band I with a SNR~9 at
2.561"\pm 0.007" (46.5 AU) from HD130948A and confirm in two independent
dataset that the object is real,as opposed to time-varying residual speckles.We
do not resolve the binary, which can be explained by astrometric results
posterior to our observations that predict a separation below the NOT
resolution.We reach at this distance a contrast of dI = 11.30 \pm 0.11, and
estimate a combined magnitude for this binary to I = 16.49 \pm 0.11 and a I-J
colour 3.29 \pm 0.13. At 1", we reach a detectability 10.5 mag fainter than the
primary after image post-processing. We obtain on-sky validation of a technique
based on speckle imaging and wavelet-transform processing,which improves the
high contrast capabilities of speckle imaging.The I-J colour measured for the
BD companion is slightly bluer, but still consistent with what typically found
for L2 dwarfs(~3.4-3.6).Comment: accepted in A\&
A Cautionary Tale: MARVELS Brown Dwarf Candidate Reveals Itself To Be A Very Long Period, Highly Eccentric Spectroscopic Stellar Binary
We report the discovery of a highly eccentric, double-lined spectroscopic
binary star system (TYC 3010-1494-1), comprising two solar-type stars that we
had initially identified as a single star with a brown dwarf companion. At the
moderate resolving power of the MARVELS spectrograph and the spectrographs used
for subsequent radial-velocity (RV) measurements (R ~ <30,000), this particular
stellar binary mimics a single-lined binary with an RV signal that would be
induced by a brown dwarf companion (Msin(i)~50 M_Jup) to a solar-type primary.
At least three properties of this system allow it to masquerade as a single
star with a very low-mass companion: its large eccentricity (e~0.8), its
relatively long period (P~238 days), and the approximately perpendicular
orientation of the semi-major axis with respect to the line of sight (omega~189
degrees). As a result of these properties, for ~95% of the orbit the two sets
of stellar spectral lines are completely blended, and the RV measurements based
on centroiding on the apparently single-lined spectrum is very well fit by an
orbit solution indicative of a brown dwarf companion on a more circular orbit
(e~0.3). Only during the ~5% of the orbit near periastron passage does the
true, double-lined nature and large RV amplitude of ~15 km/s reveal itself. The
discovery of this binary system is an important lesson for RV surveys searching
for substellar companions; at a given resolution and observing cadence, a
survey will be susceptible to these kinds of astrophysical false positives for
a range of orbital parameters. Finally, for surveys like MARVELS that lack the
resolution for a useful line bisector analysis, it is imperative to monitor the
peak of the cross-correlation function for suspicious changes in width or
shape, so that such false positives can be flagged during the candidate vetting
process.Comment: 16 pages, 11 figures, 6 table
Physical fitness reference standards for preschool children: The PREFIT project
Objectives
Reference values are necessary for classifying children, for health screening, and for early prevention as many non-communicable diseases aggravate during growth and development. While physical fitness reference standards are available in children aged 6 and older, such information is lacking in preschool children. Therefore, the purposes of this study were (1) to provide sex-and age-specific physical fitness reference standards for Spanish preschool children; and (2) to study sex differences across this age period and to characterise fitness performance throughout the preschool period.
Design
Cross-sectional.
Methods
A total of 3179 preschool children (1678 boys) aged 2.8–6.4 years old from Spain were included in the present study. Physical fitness was measured using the PREFIT battery.
Results
Age- and sex-specific percentiles for the physical fitness components are provided. Boys performed better than girls in the cardiorespiratory fitness, muscular strength, and speed-agility tests over the whole preschool period studied and for the different percentiles. In contrast, girls performed slightly better than boys in the balance test. Older children had better performance in all fitness tests than their younger counterparts.
Conclusions
Our study provides age- and sex-specific physical fitness reference standards in preschool children allowing interpretation of fitness assessment. Sexual dimorphism in fitness tests exists already at preschool age, and these differences become larger with age. These findings will help health, sport, and school professionals to identify preschool children with a high/very low fitness level, to examine changes in fitness over time, and to analyse those changes obtained due to intervention effects
A larger brown fat volume and lower radiodensity are related to a greater cardiometabolic risk, especially in young men
Objectives: Brown adipose tissue (BAT) is important in the maintenance of cardiometabolic health in rodents. Recent reports appear to suggest the same in humans, although if this is true remains elusive partly because of the methodological bias that affected previous research. This cross-sectional work reports the relationships of cold-induced BAT volume, activity (peak standardized uptake, SUVpeak), and mean radiodensity (an inverse proxy of the triacylglycerols content) with the cardiometabolic and inflammatory profile of 131 young adults, and how these relationships are influenced by sex and body weight.Design: This is a cross-sectional study.Methods: Subjects underwent personalized cold exposure for 2 h to activate BAT, followed by static F-18-fluorodeoxyglucose PET-CT scanning to determine BAT variables. Information on cardiometabolic risk (CMR) and inflammatory markers was gathered, and a CMR score and fatty liver index (FLI) were calculated.Results: In men, BAT volume was positively related to homocysteine and liver damage markers concentrations (independently of BMI and seasonality) and the FLI (all P 0.05).Conclusions: A larger BAT volume and a lower BAT mean radiodensity are related to a higher CMR, especially in young men, which may support that BAT acts as a compensatory organ in states of metabolic disruption.</p
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