Video playback demonstrates episodic personality in the gloomy octopus

Coleoid cephalopods, including octopuses, cuttlefish and squid, rely mainly on visual signals when interacting with conspecifics, predators and prey. Presenting visual stimuli, such as models, photographs, mirrors and live conspecifics, can thus provide insight into cephalopod behaviour. These methods, however, have limitations – mirrors and live animals lack experimental control, whereas models and photographs sacrifice motion-based information. Video playback addresses these issues by presenting controlled, moving and realistic stimuli but, to date, video playback has not been used successfully with any cephalopod. Here, we developed a video playback technique for the gloomy octopus (Octopus tetricus) that incorporated recent advances in video technology. We then used this technique to test for personality, which we defined as behavioural differences between individuals that are consistent over time and across ecologically important contexts. We captured wild octopuses and tested them on 3 separate days over a 10 day period. On each test day, subjects were presented with videos of a food item, a novel object and a conspecific. These represented a foraging, novel and threatening context, respectively. A fourth video without a moving stimulus controlled for the playback monitor itself and potential artifacts associated with video playback. Experimental stimuli evoked unambiguous and biologically appropriate responses from the subjects. Furthermore, individuals' responses to the three experimental contexts were highly correlated within a given test day. However, within a given context, individuals behaved inconsistently across the 3 test days. The reordering of ranks suggests that rather than fulfilling the criteria for personality, gloomy octopus show temporal discontinuities, and hence display episodic personality

Melting of Polydisperse Hard Disks

The melting of a polydisperse hard disk system is investigated by Monte Carlo simulations in the semigrand canonical ensemble. This is done in the context of possible continuous melting by a dislocation unbinding mechanism, as an extension of the 2D hard disk melting problem. We find that while there is pronounced fractionation in polydispersity, the apparent density-polydispersity gap does not increase in width, contrary to 3D polydisperse hard spheres. The point where the Young's modulus is low enough for the dislocation unbinding to occur moves with the apparent melting point, but stays within the density gap, just like for the monodisperse hard disk system. Additionally, we find that throughout the accessible polydispersity range, the bound dislocation-pair concentration is high enough to affect the dislocation unbinding melting as predicted by Kosterlitz, Thouless, Halperin, Nelson and Young.Comment: 6 pages, 6 figure

Under pressure: evolutionary engineering of yeast strains for improved performance in fuels and chemicals production.

Evolutionary engineering, which uses laboratory evolution to select for industrially relevant traits, is a popular strategy in the development of high-performing yeast strains for industrial production of fuels and chemicals. By integrating whole-genome sequencing, bioinformatics, classical genetics and genome-editing techniques, evolutionary engineering has also become a powerful approach for identification and reverse engineering of molecular mechanisms that underlie industrially relevant traits. New techniques enable acceleration of in vivo mutation rates, both across yeast genomes and at specific loci. Recent studies indicate that phenotypic trade-offs, which are often observed after evolution under constant conditions, can be mitigated by using dynamic cultivation regimes. Advances in research on synthetic regulatory circuits offer exciting possibilities to extend the applicability of evolutionary engineering to products of yeasts whose synthesis requires a net input of cellular energy

Effectiveness of two web-based cognitive bias modification interventions targeting approach and attentional bias in gambling problems:Study protocol for a pilot randomised controlled trial

BACKGROUND: Disordered gamblers have phenotypical and pathological similarities to those with substance use disorders (SUD), including exaggerated automatic cognitive processing of motivationally salient gambling cues in the environment (i.e., attentional and approach bias). Cognitive bias modification (CBM) is a family of computerised interventions that have proved effective in successfully re-training these automatic cognitive biases in SUD. CBM interventions can, in principle, be administered online, thus showing potential of being a low-cost, low-threshold addition to conventional treatments. This paper presents the design of a pilot randomised controlled trial exploring the effectiveness of two web-based CBM interventions targeting attentional and approach bias towards gambling cues in a sample of Dutch and Belgian problematic and pathological gamblers. METHODS/DESIGN: Participants (N = 182) are community-recruited adults experiencing gambling problems, who have gambled at least twice in the past 6 months and are motivated to change their gambling behaviour. After a baseline assessment session, participants are randomly assigned to one of four experimental conditions (attentional or approach bias training, or the placebo version of the two trainings) and complete six sessions of training. At baseline and before each training session, participants receive automated personalised feedback on their gambling motives and reasons to quit or reduce gambling. The post-intervention, 1-month, and 3-month follow-up assessments will examine changes in gambling behaviour, with frequency and expenditure as primary outcomes, and depressive symptoms and gambling-related attentional and approach biases as secondary outcomes. Secondary analyses will explore possible moderators (interference control capacity and trait impulsivity) and mediators (change in cognitive bias) of training effects on the primary outcomes. DISCUSSION: This study is the first to explore the effectiveness of an online CBM intervention for gambling problems. The results of this study can be extremely valuable for developing e-health interventions for gambling problems and further understanding the role of motivational implicit cognitive processes underlying problematic gambling behaviour. TRIAL REGISTRATION: Netherlands Trial Register, NTR5096 . Registered on 11 March 2015

The Canadian Federation of Earth Sciences Scientific Statement on Climate Change – Its Impacts in Canada, and the Critical Role of Earth Scientists in Mitigation and Adaptation

The Canadian Federation of Earth Sciences (CFES) has issued this statement to summarize the science, effects, and implications of climate change. We highlight the role of Earth scientists in documenting and mitigating climate change, and in managing and adapting to its consequences in Canada. CFES is the coordinated voice of Canada’s Earth Sciences community with 14 member organizations representing some 15,000 geoscientists. Our members are drawn from academia, industry, education, and government. The mission of CFES is to ensure decision makers and the public understand the contributions of Earth Science to Canadian society and the economy.  Climate change has become a national and global priority for all levels of government. The geological record shows us that the global climate has changed throughout Earth’s history, but the current rates of change are almost unprecedented. Over the last 70 years, levels of common greenhouse gases (GHGs) in the atmosphere have steadily increased. Carbon dioxide (CO2) concentration is now 418 parts per million — its highest of the last three million years. The chemical (isotopic) composition of carbon in the atmosphere indicates the increase in GHGs is due to burning fossil fuels. GHGs absorb energy emitted from Earth’s surface and re-radiate it back, warming the lower levels of the atmosphere. Climatic adjustments that have recently occurred are, in practical terms, irreversible, but further change can be mitigated by lowering emissions of GHGs.  Climate change is amplified by three important Earth system processes and effects. First, as the climate warms evaporation increases, raising atmospheric concentrations of water vapour, itself a GHG — and adding to warming. Second, loss of ice cover from the polar ice sheets and glaciers exposes larger areas of land and open water — leading to greater absorption of heat from the sun. Third, thawing of near-surface permafrost releases additional GHGs (primarily CO2 and methane) during decay of organic matter previously preserved frozen in the ground. Some impacts of climate change are incremental and steadily occurring, such as melting of glaciers and ice sheets, with consequent sea level rise. Others are intermittent, such as extreme weather events, like hurricanes — but are becoming more frequent. Summer water shortages are increasingly common in western Canada as mountain snowpacks melt earlier and summer river flows decline. In northern Canada, warming and thawing of near-surface permafrost has led to deterioration of infrastructure and increased costs for buildings that now require chilled foundations. Other consequences of unchecked climate change include increased coastal erosion, increases in the number and size of wildfires, and reduction in winter road access to isolated northern communities. Reductions in net GHG emissions are urgently required to mitigate the many effects of further climate change. Industrial and public works development projects must now assess the effects of climate change in their planning, design, and management. Cities, municipalities, and rural communities need to plan new residential development carefully to avoid enhanced risk of flooding, coastal erosion, or wildfire.  Earth Science knowledge and expertise is integral to exploration and development of new metals and Earth materials required for a carbon-neutral future, and in the capture and storage of CO2 within the Earth. Earth Science is also central to society’s adaptation to new climatic regimes and reduction of risks. This includes anticipation, assessment, and management of extreme events, development of new standards and guidelines for geotechnical and engineering practice, and revision to regulations that consider climate change. Geoscientists also have an important role in the education of students and the public on the reasons for necessary action. Canada is uniquely positioned with its strong global geoscientific leadership, its vast landmass, and its northern terrain to effectively leverage research activities around climate change. Geoscience tools and geoscientists’ skills will be integral to Canada’s preparation for climate change.La Fédération canadienne des sciences de la Terre (FCST) a publié ce communiqué pour résumer la science, les effets et les implications des changements climatiques. Nous soulignons le rôle des scientifiques en science de la Terre dans la documentation et l'atténuation des changements climatiques, ainsi que dans la gestion de leurs conséquences et la création de mesures d'adaptation au Canada. La FCST est la voix coordonnée de la communauté canadienne des sciences de la Terre avec 14 organisations membres représentant environ 15 000 géoscientifiques. Nos membres sont issus du milieu universitaire, de l'industrie, de l'éducation et du gouvernement. La mission de la FCST est de s'assurer que les décideurs et le public comprennent les contributions des sciences de la Terre à la société canadienne et à l'économie.  Les changements climatiques sont devenus une priorité nationale et mondiale à tous les niveaux de gouvernement. Les archives géologiques nous montrent que le climat mondial a changé tout au long de l'histoire de la Terre, mais les taux de changement actuels sont presque sans précédent. Au cours des 70 dernières années, les niveaux de gaz à effet de serre (GES) communs dans l'atmosphère n'ont cessé d'augmenter. La concentration de dioxyde de carbone (CO2) est maintenant de 418 parties par million - son plus haut niveau des trois derniers millions d'années. La composition chimique (isotopique) du carbone dans l'atmosphère indique que l'augmentation des GES est due à la combustion de combustibles fossiles. Les GES absorbent l'énergie émise par la surface de la Terre et la réfléchissent, réchauffant les niveaux inférieurs de l'atmosphère. Les modifications climatiques qui se sont produits récemment sont, concrètement, irréversibles, mais les changements additionnels peuvent être atténués en réduisant les émissions de GES.  Les changements climatiques sont amplifiés par trois processus et effets importants du système terrestre. Premièrement, à mesure que le climat se réchauffe, l'évaporation augmente, ce qui augmente les concentrations atmosphériques de vapeur d'eau, elle-même un GES, et contribue au réchauffement. Deuxièmement, la perte de la couverture de glace des calottes glaciaires polaires et des glaciers expose de plus grandes superficies de terre et d'eau libre, ce qui entraîne une plus grande absorption de la chaleur du soleil. Troisièmement, le dégel du pergélisol proche de la surface libère des GES supplémentaires (principalement du CO2 et du méthane) lors de la décomposition de la matière organique jusqu’alors préservée gelée dans le sol. Certains impacts des changements climatiques sont progressifs et se produisent régulièrement, comme la fonte des glaciers et des calottes glaciaires, avec pour conséquence une élévation du niveau de la mer. D'autres sont intermittents, comme les événements météorologiques extrêmes, tels que les ouragans, mais deviennent de plus en plus fréquents. Les pénuries d'eau en été sont de plus en plus courantes dans l'ouest du Canada, car le manteau neigeux des montagnes fond plus tôt et le débit des rivières en été diminue. Dans le nord du Canada, le réchauffement et le dégel du pergélisol proche de la surface ont entraîné une détérioration des infrastructures et une augmentation des coûts des bâtiments qui nécessitent maintenant des fondations réfrigérées. Les autres conséquences des changements climatiques incontrôlés comprennent l'augmentation de l'érosion côtière, l'augmentation du nombre et de la taille des incendies de forêt et la réduction de l'accès aux routes d’hiver aux collectivités isolées du Nord. Des réductions des émissions nettes de GES sont nécessaires de toute urgence pour atténuer les nombreux effets de nouveaux changements climatiques. Les projets de développement industriel et de travaux publics doivent désormais évaluer les effets des changements climatiques dans leur planification, leur conception et leur gestion. Les villes, les municipalités et les communautés rurales doivent planifier soigneusement les nouveaux développements résidentiels pour éviter les risques accrus d'inondation, d'érosion côtière ou d'incendie de forêt.  Les connaissances et l'expertise en sciences de la Terre font partie intégrante de l'exploration et du développement de nouveaux métaux et matériaux terrestres requis pour un avenir neutre en carbone, ainsi que dans la capture et la séquestration du CO2 dans la Terre. Les sciences de la Terre sont également au cœur de l'adaptation de la société aux nouveaux régimes climatiques et de la réduction des risques. Cela comprend l'anticipation, l'évaluation et la gestion des événements extrêmes, l'élaboration de nouvelles normes et directives pour les pratiques géotechniques et d'ingénierie, et la révision des réglementations qui tient compte des changements climatiques. Les géoscientifiques ont également un rôle important dans l'éducation des étudiants et du public sur le fondement des mesures nécessaires. Le Canada occupe une position unique grâce à son solide leadership géoscientifique mondial, sa vaste étendue et son territoire nordique pour tirer efficacement parti des activités de recherche sur les changements climatiques. Les outils géoscientifiques et les compétences des géoscientifiques feront partie intégrante de la préparation du Canada aux changements climatiques

Characteristics of Adults Who Use Prayer as an Alternative Therapy

Purpose: To describe the demographics, health-related and preventive-health behaviors, health status, and health care charges of adults who do and do not pray for health. Design: Cross-sectional survey with 1-year follow-up. Setting: A Minnesota health plan. Subjects: A stratified random sample of 5107 members age 40 and over with analysis based on 4404 survey respondents (86%). Measures: Survey data included health risks, health practices, use of preventive health services, satisfaction with care, and use of alternative therapies. Health care charges were obtained from administrative data. Results: Overall, 47.2% of study subjects reported that they pray for health, and 90.3% of these believed prayer improved their health. After adjustment for demographics, those who pray had significantly less smoking and alcohol use and more preventive care visits, influenza immunizations, vegetable intake, satisfaction with care, and social support and were more likely to have a regular primary care provider. Rates of functional impairment, depressive symptoms, chronic diseases, and total health care charges were not related to prayer. Conclusions: Those who pray had more favorable health-related behaviors, preventive service use, and satisfaction with care. Discussion of prayer could help guide customization of clinical care. Research that examines the effect of prayer on health status should adjust for variables related both to use of prayer and to health status

Multiple verification in computational modeling of bone pathologies

We introduce a model checking approach to diagnose the emerging of bone pathologies. The implementation of a new model of bone remodeling in PRISM has led to an interesting characterization of osteoporosis as a defective bone remodeling dynamics with respect to other bone pathologies. Our approach allows to derive three types of model checking-based diagnostic estimators. The first diagnostic measure focuses on the level of bone mineral density, which is currently used in medical practice. In addition, we have introduced a novel diagnostic estimator which uses the full patient clinical record, here simulated using the modeling framework. This estimator detects rapid (months) negative changes in bone mineral density. Independently of the actual bone mineral density, when the decrease occurs rapidly it is important to alarm the patient and monitor him/her more closely to detect insurgence of other bone co-morbidities. A third estimator takes into account the variance of the bone density, which could address the investigation of metabolic syndromes, diabetes and cancer. Our implementation could make use of different logical combinations of these statistical estimators and could incorporate other biomarkers for other systemic co-morbidities (for example diabetes and thalassemia). We are delighted to report that the combination of stochastic modeling with formal methods motivate new diagnostic framework for complex pathologies. In particular our approach takes into consideration important properties of biosystems such as multiscale and self-adaptiveness. The multi-diagnosis could be further expanded, inching towards the complexity of human diseases. Finally, we briefly introduce self-adaptiveness in formal methods which is a key property in the regulative mechanisms of biological systems and well known in other mathematical and engineering areas.Comment: In Proceedings CompMod 2011, arXiv:1109.104