431 research outputs found
Stellungnahme des Max-Planck-Instituts fĂźr Geistiges Eigentum, Wettbewerbs- und Steuerrecht zum Vorschlag fĂźr eine Richtlinie des Europäischen Parlaments und des Rates Ăźber strafrechtliche MaĂnahmen zur Durchsetzung des Rechts des geistigen Eigentums, KOM(2006) 168 endgĂźltig
Carboxylate ion pairing with alkali-metal ions for β-Lactoglobulin and its role on aggregation and interfacial adsorption
We
report a combined experimental and computational study of the
whey protein β-lactoglobulin (BLG) in different electrolyte
solutions. Vibrational sum-frequency generation (SFG) and ellipsometry
were used to investigate the molecular structure of BLG modified airâwater
interfaces as a function of LiCl, NaCl, and KCl concentrations. Molecular
dynamics (MD) simulations and thermodynamic integration provided details
of the ion pairing of protein surface residues with alkali-metal cations.
Our results at pH 6.2 indicate that BLG at the airâwater interface
forms mono- and bilayers preferably at low and high ionic strength,
respectively. Results from SFG spectroscopy and ellipsometry are consistent
with intimate ion pairing of alkali-metal cations with aspartate and
glutamate carboxylates, which is shown to be more effective for smaller
cations (Li<sup>+</sup> and Na<sup>+</sup>). MD simulations show not
only carboxylateâalkali-metal ion pairs but also ion multiplets
with the alkali-metal ion in a bridging position between two or more
carboxylates. Consequently, alkali-metal cations can bridge carboxylates
not only within a monomer but also between monomers, thus providing
an important dimerization mechanism between hydrophilic surface patches
Measurement of length distribution of beta-lactoglobulin fibrils by multiwavelength analytical ultracentrifugation
The whey protein beta-lactoglobulin is the building block of amyloid fibrils which exhibit a great potential in various applications. These include stabilization of gels or emulsions. During biotechnological processing, high shear forces lead to fragmentation of fibrils and therefore to smaller fibril lengths. To provide insight into such processes, pure straight amyloid fibril dispersions (prepared at pH 2) were produced and sheared using the rotor stator setup of an Ultra Turrax. In the first part of this work, the sedimentation properties of fragmented amyloid fibrils sheared at different stress levels were analyzed with mulitwavelength analytical ultracentrifugation (AUC). Sedimentation data analysis was carried out with the boundary condition that fragmented fibrils were of cylindrical shape, for which frictional properties are known. These results were compared with complementary atomic force microscopy (AFM) measurements. We demonstrate how the sedimentation coefficient distribution from AUC experiments is influenced by the underlying length and diameter distribution of amyloid fibrils. In the second part of this work, we show how to correlate the fibril size reduction kinetics with the applied rotor revolution and the resulting energy density, respectively, using modal values of the sedimentation coefficients obtained from AUC. Remarkably, the determined scaling laws for the size reduction are in agreement with the results for other material systems, such as emulsification processes or the size reduction of graphene oxide sheets.</p
Synthesis and characterization of manganese containing mesoporous bioactive glass nanoparticles for biomedical applications
Mesoporous bioactive glass (BG) nanoparticles based in the system: SiO2âP2O5âCaOâMnO were synthesized via a modified
StĂśber process at various concentrations of Mn (0â7 mol %). The synthesized manganese-doped BG nanoparticles were
characterized in terms of morphology, composition, in vitro bioactivity and antibacterial activity. Scanning electron
microscopy (SEM), transmission electron microscopy (TEM) and BrunauerâEmmettâTeller (BET) analysis confirmed that
the particles had spherical morphology (mean particle size: 110 nm) with disordered mesoporous structure. Energy
dispersive X-ray spectroscopy (EDX) confirmed the presence of Mn, Ca, Si and P in the synthesized Mn-doped BG particles.
Moreover, X-ray diffraction (XRD) analysis showed that Mn has been incorporated in the amorphous silica network
(bioactive glass). Moreover, it was found that manganese-doped BG particles form apatite crystals upon immersion in
simulated body fluid (SBF). Inductively coupled plasma atomic emission spectroscopy (ICP-OES) measurements confirmed
that Mn is released in a sustained manner, which provided antibacterial effect against Bacillus subtilis, Pseudomonas
aeruginosa and Staphylococcus aureus. The results indicate that the incorporation of Mn in the bioactive glass network is an
effective strategy to develop novel multifunctional BG nanoparticles for bone tissue engineering.University of Seville (Spain) Grant P2017/83
Grain Structure Evolution of AlâCu Alloys in Powder Bed Fusion with Laser Beam for Excellent Mechanical Properties
Powder Bed Fusion with Laser Beam of Metals (PBF-LB/M) is one of the fastest growing technology branches. More and more metallic alloys are being qualified, but processing of aluminum wrought alloys without cracks and defects is still challenging. It has already been shown that small parts with low residual porosity can be produced. However, suffering from microscopic hot cracks, the fracture behavior has been rather brittle. In this paper different combinations of temperature gradients and solidification rates are used to achieve specific solidification conditions in order to influence the resulting microstructure, as well as internal stresses. By this approach it could be shown that EN AW-2024, an aluminum-copper wrought alloy, is processable via PBF-LB/M fully dense and crack-free with outstanding material properties, exceeding those reported for commonly manufactured EN AW-2024 after T4 heat treatment
Spatial variation in soil properties and diffuse losses between and within grassland fields with similar short-term management
One of the major challenges for agriculture is to understand the effects of agricultural practices on soil properties and diffuse pollution, to support practical farm-scale land management. Three conventionally managed grassland fields with similar short-term management, but different ploughing histories, were studied on a long-term research platform: the North Wyke Farm Platform. The aims were to (i) quantify the between-field and within-field spatial variation in soil properties by geostatistical analysis, (ii) understand the effects of soil condition (in terms of nitrogen, phosphorus and carbon contents) on the quality of discharge water and (iii) establish robust baseline data before the implementation of various grassland management scenarios. Although the fields sampled had experienced the same land use and similar management for at least 6 years, there were differences in their mean soil properties. They showed different patterns of soil spatial variation and different rates of diffuse nutrient losses to water. The oldest permanent pasture field had the largest soil macronutrient concentrations and the greatest diffuse nutrient losses. We show that management histories affect soil properties and diffuse losses. Potential gains in herbage yield or benefits in water quality might be achieved by characterizing every field or by area-specific management within fields (a form of precision agriculture for grasslands). Permanent pasture per se cannot be considered a mitigation measure for diffuse pollution. The between-and within-field soil spatial variation emphasizes the importance of baseline characterization and will enable the reliable identification of any effects of future management change on the Farm Platform
Biophysical Investigation of the Mode of Inhibition of Tetramic Acids, the Allosteric Inhibitors of Undecaprenyl Pyrophosphate Synthase
Undecaprenyl pyrophosphate synthase (UPPS) catalyzes the consecutive condensation of eight molecules of isopentenyl pyrophosphate (IPP) with farnesyl pyrophosphate (FPP) to generate the C(55) undecaprenyl pyrophosphate (UPP). It has been demonstrated that tetramic acids (TAs) are selective and potent inhibitors of UPPS, but the mode of inhibition was unclear. In this work, we used a fluorescent FPP probe to study possible TA binding at the FPP binding site. A photosensitive TA analogue was designed and synthesized for the study of the site of interaction of TA with UPPS using photo-cross-linking and mass spectrometry. The interaction of substrates with UPPS and with the UPPS.TA complex was investigated by protein fluorescence spectroscopy. Our results suggested that tetramic acid binds to UPPS at an allosteric site adjacent to the FPP binding site. TA binds to free UPPS enzyme but not to substrate-bound UPPS. Unlike Escherichia coli UPPS which follows an ordered substrate binding mechanism, Streptococcus pneumoniae UPPS appears to follow a random-sequential substrate binding mechanism. Only one substrate, FPP or IPP, is able to bind to the UPPS.TA complex, but the quaternary complex, UPPS.TA.FPP.IPP, cannot be formed. We propose that binding of TA to UPPS significantly alters the conformation of UPPS needed for proper substrate binding. As the result, substrate turnover is prevented, leading to the inhibition of UPPS catalytic activity. These probe compounds and biophysical assays also allowed us to quickly study the mode of inhibition of other UPPS inhibitors identified from a high-throughput screening and inhibitors produced from a medicinal chemistry program
Comment of the European copyright society. Addressing selected aspects of the implementation of articles 18 to 22 of the directive (EU) 2019/790 on copyright in the digital single market
Phosphate stable oxygen isotope variability within a temperate agricultural soil
In this study, we conduct a spatial analysis of soil total phosphorus (TP), acid extractable phosphate (PO4) and the stable oxygen (O) isotope ratio within the PO4 molecule (δ18OPO4) from an intensively managed agricultural grassland site. Total P in the soil was found to range from 736 to 1952 mg P kgâ 1, of which between 12 and 48% was extractable using a 1 M HCl (HClPO4) solution with the two variables exhibiting a strong positive correlation. The δ18OPO4 of the extracted PO4 ranged from 17.0 to 21.6â° with a mean of 18.8â° (Âą 0.8). While the spatial variability of Total P has been researched at various scales, this is the first study to assess the variability of soil δ18OPO4 at a field-scale resolution. We investigate whether or not δ18OPO4 variability has any significant relationship with: (i) itself with respect to spatial autocorrelation effects; and (ii) HClPO4, elevation and slope - both globally and locally. Results indicate that δ18OPO4 was not spatially autocorrelated; and that δ18OPO4 was only weakly related to HClPO4, elevation and slope, when considering the study field as a whole. Interestingly, the latter relationships appear to vary in strength locally. In particular, the δ18OPO4 to HClPO4 relationship may depend on the underlying soil class and/or on different field managements that had operated across an historical north-south field division of the study field, a division that had been removed four years prior to this study
Towards recombinantly produced milk proteins: Physicochemical and emulsifying properties of engineered whey protein beta-lactoglobulin variants
DFG, 273937032, SPP 1934: Dispersitäts-, Struktur- und Phasenänderungen von Proteinen und biologischen Agglomeraten in biotechnologischen ProzessenBMBF, 031B0222, Basistechnologie Nachwuchsgruppe "Multiskalige Modellierung und Modifikation von Multienzymkomplexen als Basistechnologie fßr zellfreie Reaktionskaskaden" (II
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