Datasets for transcriptomics, q-proteomics and phenotype microarrays of polyphosphate metabolism mutants from Escherichia coli

Indexación: Scopus.Author acknowledges Fondecyt Grants 1120209, 1121170 and Anillo ACT-1107Here, we provide the dataset associated with our research article on the polyphosphate metabolism entitled, “Multi-level evaluation of Escherichia coli polyphosphate related mutants using global transcriptomic, proteomic and phenomic analyses”. By integrating different omics levels (transcriptome, proteome and phenome), we were able to study Escherichia coli polyphosphate mutant strains (Δppk1, Δppx, and Δppk1-ppx). We have compiled here all datasets from DNA microarrys, q-proteomic (Isotope-Coded Protein Labeling, ICPL) and phenomic (Phenotype microarray) raw data we have obtained in all polyP metabolism mutants.http://www.sciencedirect.com/science/article/pii/S2352340917300860?via%3Dihu

Evaluación actual y propuestas para el desarrollo urbano en México

La revista Problemas del Desarrollo recoge en su colección de ediciones especiales las disertaciones y ponencias que se presentan en los seminarios, simposia y conferencias a que convoca y realiza anualmente el Instituto de Investigaciones Económicas de la Universidad Nacional Autónoma de México. El propósito es contribuir a la mayor difusión de textos de contenido analítico, descriptivo y propositivo que se presentan en tales actos y que tienen gran valor para el mejor conocimiento y comprensión de la estructura y problemática económica y social de nuestro país. Pero también la revista quiere convertirse en foro abierto para la expresión de comentarios y ampliaciones que deseen hacer los lectores de estas ediciones especiales sobre temas particulares de los comprendidos en cada uno de sus libros. Estos aportes, por lo tanto, serán muy bien recibidos. Para ello, solo se requiere que los lectores los remitan, por escrito, al Instituto de Investigaciones Económicas, Torre II de Humanidades, Ciudad Universitaria

Effect of plant growth-promoting bacteria and inoculation media in the yield on cooksfoot yield

Objetives: To determine the effect of three PGPB bacteria (Ewingella americana, Bacillus simplex y Microbacterium ginsengiterrae) and three inoculant methods (digestate, compost and cornstarch) on yield, plant height, leaf temperature, tiller density and morphological composition of cooksfoot. Desing/methodology/approach: A completely randomized design was used, with a factorial arrangement 4*3, the experimental units being a pot with five stems. Results: The dry matter did not register difference between treatments with bacteria (P>0.05), but with respect to the control the values increased up to 50%. Compost and digestate media outperformed cornstarch by 22%. The inoculation media had an effect (P<0.05) on the yield and leaf, while the bacteria promoted the formation of new tillers, the yield and reduced the leaf temperature. Findings/conclusions: Compost and digestate as inoculation media can notably favor the beneficial effect of M. ginsengiterrae, which was the one that registered the best yieldsObjective: To determine the effect of three PGPB bacteria (Ewingella americana, Bacillus simplex and Microbacterium ginsengiterrae) and three inoculation media (digestate, compost and cornstarch) on plant height, leaf temperature, stalkdensity, morphological composition, and dry matter yield of orchard grass.Design/Methodology/Approach: A completely randomized design was used, with a factorial arrangement 4X3, the experimental units being a pot with five initial stalks.Results: The dry matter did not show difference between treatments with bacteria (P>0.05), but the control the values increased up to 50%. Compost and digestate media outperformed cornstarch by 22%. The inoculation media had an effect(P?0.05) on the yield and leaf variables, while the bacteria promoted the formation of new stalks and the production of  biomass, and reduced the leaf temperature.Findings/Conclusions: Compost and digestate as inoculation media can notably favor the beneficial effect of M. ginsengiterrae, which was the one that registered the best yield

Statistical Analysis of Factors Influencing Corrosion in Concrete Structures

The use of mathematical and statistical models has allowed the description of the behavior of many natural phenomena. However, their application in civil engineering particularly, for the analysis of the corrosion behavior, has been overlooked in recent studies. In the present work, a factorial analysis with subdivided parcels design were conducted to evaluate the corrosion rate behavior of arrangements of protected and not protected steel bars found in reinforced concrete specimens subject to two different aggressive media. The results showed that, in comparison to sulphate ions, the effect of chloride ions on the corrosion rate was not statistically significant. In the same way, protected and not protected segments on the steel bars (i.e., steel bar condition) did not have a significant effect on the corrosion rate. The only factor found to significantly affect the corrosion rate was the variability of the beam fabrication process (i.e., beam factor)

Peripheral T-lymphocytes express WNT7A and its restoration in leukemia-derived lymphoblasts inhibits cell proliferation

<p>Abstract</p> <p>Background</p> <p>WNT7a, a member of the Wnt ligand family implicated in several developmental processes, has also been reported to be dysregulated in some types of tumors; however, its function and implication in oncogenesis is poorly understood. Moreover, the expression of this gene and the role that it plays in the biology of blood cells remains unclear. In addition to determining the expression of the <it>WNT7A </it>gene in blood cells, in leukemia-derived cell lines, and in samples of patients with leukemia, the aim of this study was to seek the effect of this gene in proliferation.</p> <p>Methods</p> <p>We analyzed peripheral blood mononuclear cells, sorted CD3 and CD19 cells, four leukemia-derived cell lines, and blood samples from 14 patients with Acute lymphoblastic leukemia (ALL), and 19 clinically healthy subjects. Reverse transcription followed by quantitative Real-time Polymerase chain reaction (qRT-PCR) analysis were performed to determine relative <it>WNT7A </it>expression. Restoration of WNT7a was done employing a lentiviral system and by using a recombinant human protein. Cell proliferation was measured by addition of WST-1 to cell cultures.</p> <p>Results</p> <p>WNT7a is mainly produced by CD3 T-lymphocytes, its expression decreases upon activation, and it is severely reduced in leukemia-derived cell lines, as well as in the blood samples of patients with ALL when compared with healthy controls (<it>p </it>≤0.001). By restoring <it>WNT7A </it>expression in leukemia-derived cells, we were able to demonstrate that WNT7a inhibits cell growth. A similar effect was observed when a recombinant human WNT7a protein was used. Interestingly, restoration of <it>WNT7A </it>expression in Jurkat cells did not activate the canonical Wnt/β-catenin pathway.</p> <p>Conclusions</p> <p>To our knowledge, this is the first report evidencing quantitatively decreased <it>WNT7A </it>levels in leukemia-derived cells and that <it>WNT7A </it>restoration in T-lymphocytes inhibits cell proliferation. In addition, our results also support the possible function of <it>WNT7A </it>as a tumor suppressor gene as well as a therapeutic tool.</p

Local and Landscape Factors Determining Occurrence of Phyllostomid Bats in Tropical Secondary Forests

Neotropical forests are being increasingly replaced by a mosaic of patches of different successional stages, agricultural fields and pasture lands. Consequently, the identification of factors shaping the performance of taxa in anthropogenic landscapes is gaining importance, especially for taxa playing critical roles in ecosystem functioning. As phyllostomid bats provide important ecological services through seed dispersal, pollination and control of animal populations, in this study we assessed the relationships between phyllostomid occurrence and the variation in local and landscape level habitat attributes caused by disturbance. We mist-netted phyllostomids in 12 sites representing 4 successional stages of a tropical dry forest (initial, early, intermediate and late). We also quantitatively characterized the habitat attributes at the local (vegetation structure complexity) and the landscape level (forest cover, area and diversity of patches). Two focal scales were considered for landscape characterization: 500 and 1000 m. During 142 sampling nights, we captured 606 individuals representing 15 species and 4 broad guilds. Variation in phyllostomid assemblages, ensembles and populations was associated with variation in local and landscape habitat attributes, and this association was scale-dependent. Specifically, we found a marked guild-specific response, where the abundance of nectarivores tended to be negatively associated with the mean area of dry forest patches, while the abundance of frugivores was positively associated with the percentage of riparian forest. These results are explained by the prevalence of chiropterophilic species in the dry forest and of chiropterochorous species in the riparian forest. Our results indicate that different vegetation classes, as well as a multi-spatial scale approach must be considered for evaluating bat response to variation in landscape attributes. Moreover, for the long-term conservation of phyllostomids in anthropogenic landscapes, we must realize that the management of the habitat at the landscape level is as important as the conservation of particular forest fragments

Comunidades, territorios y turismo en América Latina

Este libro es una suerte de regreso al futuro en la medida que intenta explicar la historia reciente del desarrollo turístico como parte de los males del capitalismo neoliberal globalizado, y al mismo tiempo tornar palpable las ventajas y oportunidades de ese otro turismo, enraizado en los paisajes naturales y culturales de las comunidades locales