Hierarchical factor structure of the Intolerance of Uncertainty Scale short form (IUS-12) in the Italian version

Despite widespread use, few translations are available for the Intolerance of Uncertainty Scale short form (IUS-12) as well as limited research on its psychometric properties in Italy. Moreover, recent evidence has suggested a multifaceted hierarchical structure for this scale. We compared the two-factor model to second-order and bi-factor models, in which a General IU factor was posited with two more narrow factors: Prospective IU and Inhibitory IU. Models were tested on a pooled dataset of students (N = 609) taking the IUS-12 alone or with other IUS-27 items. The bi-factor model fitted the sample data better than alternative models. The general factor accounted for 80% of the item variance. Presentation mode did not impact scalar invariance. Convergent validity with neuroticism, need for closure, and the uncertainty response scale was high for the total score. As such, scoring the IUS-12 total score is recommended in clinical research and assessmen

Involvement of caspace-3 in the cleavage of terminal transferase.

To investigate the in vivo role of caspase-3 in Terminal Transferase metabolism DMSO-treated RPMI-8402, a human pre-T cell line was used. In DMSO treated samples3H-dGTP incorporation and TdT phosphorylation occurs after 4 hours of treatment. After 8 hours cells undergo TdT proteolysis in addition to its inactivation. The cleavage of TdT into 32- and 58-KDa proteolytic fragments occurred simultaneously with the activation of Caspase-3, but preceded changes associated with the apoptotic process described after 48 hours of treatment. The Caspase-3 peptide inhibitor V, used as a specific inhibitor, prevented TdT proteolysis prolonging its activity and rescued cells from apoptosis. Our experiments suggest that TdT is a nuclear substrate for Caspase-3, the main apoptotic effector protease in many cell types, and that the cleavage of TdT represents a primary step in a signal cascade leading to pre-T cell apoptosis

A correlational analysis of the relationships among intolerance of uncertainty, anxiety sensitivity, subjective sleep quality, and insomnia symptoms

In this study, we used structural equation modeling to investigate the interplay among Intolerance of Uncertainty (IU), Anxiety Sensitivity (AS), and sleep problems. Three hundred undergraduate students completed the Intolerance of Uncertainty Scale, the Intolerance of Uncertainty Inventory, the Anxiety Sensitivity Index, the Beck Depression Inventory, the State-Trait Anxiety Inventory, the Pittsburgh Sleep Quality Index and the Insomnia Severity Index. 68% and 40% of the students reported poor sleep quality or sub-threshold insomnia problems, respectively. Depression and anxiety levels were above the cut-off for about one-fourth of the participants. Structural equation modeling revealed that IU was strongly associated with AS, in turn influencing both insomnia severity and sleep quality via depression and anxiety. Significant indirect effects revealed that an anxious pathway was more strongly associated with insomnia severity, while a depression pathway was more relevant for worsening the quality of sleep. We discussed the results in the frameworks of cognitive models of insomnia. Viewing AS and IU as antecedents of sleep problems and assigning to AS a pivotal role, our study suggested indications for clinical interventions on a population at risk for sleep disorders

Diversity assessment of seedlings from self-pollinated Sangiovese grapevines by ampelography and microsatellite DNA analysis

A population of Vitis vinifera L. seedlings deriving from a single self-pollinated Sangiovese vine were assayed for diversity by ampelographic and genetic techniques. After field-transplantation in 1987, the seedlings were initially screened in 1995. Twenty-four seedlings were of standard Vigour and grape production. Woody cuttings from the 24 seedlings and the mother plant were self-rooted in 1995, and each Vine was morphologically analysed and compared in 1997 using 31 descriptors of the ampelographic data sheet (OIV 1983) which are also recommended by UPOV for varietal identification - three for young shoots, 7 for shoots at bloom, 17 for adult leaves, one for flowers and 3 for berries. - In 1996 DNA was extracted from young apical leaves of the mother plant and the 24 seedlings. Ten molecular microsatellites, VVS1, VVS2, VVS5, VVS16, VVS29, VVMD5, VVMD6, VVMD7, VVMD17 and VVMD28, were used for progeny and mother plant comparison. The descriptor-based analysis showed that 12 of the 24 seedlings were morphologically similar amongst themselves and not different fr-om the mother; the remaining 12 differing from each other and from the parent. The microsatellite analysis differentiated all 24 seedlings from the mother plant. Only two seedlings showed the same allele patterns at the 10 tested loci, although they differed morphologically. The results of both analyses indicate that self-pollination can generate phenotypically similar individuals that are difficult to distinguish morphologically, while their genetic polymorphism can easily be detected by microsatellite analysis. Thus it is possible, as suggested by RIVES (1961), that certain ancient cultivars comprise a number of clones that derive via vegetative propagation from closely related mother plants. Corroboration of the polyclonal origin in such cases can be performed by techniques combining morphological and molecular approaches

千葉医学雑誌 88巻3号 表紙 : 目次.千葉医学雑誌 88巻 3号

<p>Mediation analysis.</p

Peningkatan Kemampuan Pengelolaan USAha Pelaku USAha Mikro di Sekitar UNPAR dengan Pemberian Pelatihan dan Membangun Jejaring

Pengabdian pada Masyarakat Jurusan Manajemen UNPAR yang telah dilakukan selama ini terhadap warga yang merupakan ibu-ibu PKK maupun pelaku USAha di RW 11 dirasa telah berjalan dengan baik. Para peserta selalu antusias dalam mengikuti pelatihan yang diberikan, dan mereka menjadi lebih mengetahui apa yang seharusnya dilakukan ataupun tidak dilakukan berhubungan dengan USAha mereka ataupun terhadap pengelolaan rumah tangganya.Pelatihan yang telah dilakukan sehubungan dengan kegiatan Pengabdian pada Masyarakat Jurusan manajemen adalah pelatihan pembukuan, pelatihan perhitungan harga pokok, diskusi tentang menabung dan investasi, pelatihan pemasaran, pemberian inspirasi tentang menjadi pelaku bisnis yang work smart, serta beberapa pelatihan yang berhubungan dengan peningkatan keahlian.Hasil yang didapat saat ini adalah para peserta merasa ditambah pengetahuannya, digugah kesadarannya, dan ditambah keahliannya. Walaupun pasti ada kendala, tetapi lebih banyak benefit yang didapat baik dari sisi peserta maupun dari sisi Jurusan Manajemen yang memberikan pelatihan dan melakukan pengembangan.Diharapkan kedepannya kegiatan ini terus berkesinambungan dan terus memberikan arti bagi kedua belah pihak, yaitu UNPAR dan masyarakat di RW 11. Selain itu diharapkan akan banyak pihak-pihak lain yang mau terlibat dalam kegiatan pengabdian ini, terutama pihak-pihak dari eksternal UNPAR, sehingga kegiatan ini dapat memunculkan banyak jejaring bagi semua pihak

Expression of CD38 in human neuroblastoma SH-SY5Y cells.

Human CD38 antigen is a 42–45 kDa type II transmembrane glycoprotein with a short N-terminal cytoplasmic domain and a long C-terminal extracellular region. It is widely expressed in different cell types including thymocytes, activated T cells, and terminally differentiated B cells (plasma cells) and it is involved in cellular proliferation and adhesion. CD38 acts as an ectocyclase that converts NAD+ to the Ca2+-releasing second messenger cyclic ADP-ribose (cADPR). It has been also demonstrated that increased extracellular levels of NAD+ and cADPR are involved in inflammatory diseases and in cellular damage, such as ischemia. In the present study, we have characterized the expression of CD38 in human neuroblastoma SH-SY5Y cell line. All-trans-retinoic acid (ATRA) treatment was used to induce cell differentiation. Our results indicate that: a) even if SH-SY5Y cells have a negative phenotype express CD38 at nuclear level, ATRA treatment does not influence this pattern; b) CD38 localizing to the nucleus may co-localize with p80-coilin positive nuclear-coiled bodies; c) purified nuclei, by Western blot determinations using anti-CD38 antibodies, display a band with a molecular mass of −42 kDa; d) SH-SY5Y cells show nuclear ADP-ribosyl cyclase due to CD38 activity; e) the basal level of CD38 mRNA shows a time-dependent increase after treatment with ATRA. These results suggest that the presence of constitutive fully functional CD38 in the SH-SY5Y nucleus has some important implications for intracellular generation of cADP-ribose and subsequent nucleoplasmic calcium release

Design of an Instrument to Evaluate Organizational Design Characteristics Based on the Organizational Configuration Model

The purpose of this article was to design and validate an instrument to determine relevant variables of the business environment, demeanor, and organizational behaviors linked to the Organizational Configuration Model and its relevance in these tumultuous times driven by the pandemic. From the literature, fifty-nine organizational attributes were extracted and administered to a random sample of 160 managers. Using a combined approach, i.e., exploratory factor analysis and construct validity, a structure of eight factors were extracted that explained 66.3 percent of the total variance with an exhibited satisfactory sample adequacy for the factor analysis (KMO = 0.63). An instrument was designed with these eight factors that provided a focused and valid perspective capable of assessing the impact and continued viability of a business’s organizational configuration under unsettled economic times. © Common Ground Research Networks, Ronald R. Rojas, Oriana Susana Martinez Palomino, Jorge Luis Villalba Acevedo, Alberto Emilio Gómez Torres, All Rights Reserved