Container seal for dusty environment

Method maintains cleanliness of joint-sealing surfaces under dust-laden conditions. This is accomplished by keeping seal and sealing surface covered with sliding plastic rings, which slide out of the way when a joint is seated

Calibrating and Stabilizing Spectropolarimeters with Charge Shuffling and Daytime Sky Measurements

Well-calibrated spectropolarimetry studies at resolutions of $R>$10,000 with signal-to-noise ratios (SNRs) better than 0.01\% across individual line profiles, are becoming common with larger aperture telescopes. Spectropolarimetric studies require high SNR observations and are often limited by instrument systematic errors. As an example, fiber-fed spectropolarimeters combined with advanced line-combination algorithms can reach statistical error limits of 0.001\% in measurements of spectral line profiles referenced to the continuum. Calibration of such observations is often required both for cross-talk and for continuum polarization. This is not straightforward since telescope cross-talk errors are rarely less than $\sim$1\%. In solar instruments like the Daniel K. Inouye Solar Telescope (DKIST), much more stringent calibration is required and the telescope optical design contains substantial intrinsic polarization artifacts. This paper describes some generally useful techniques we have applied to the HiVIS spectropolarimeter at the 3.7m AEOS telescope on Haleakala. HiVIS now yields accurate polarized spectral line profiles that are shot-noise limited to 0.01\% SNR levels at our full spectral resolution of 10,000 at spectral sampling of $\sim$100,000. We show line profiles with absolute spectropolarimetric calibration for cross-talk and continuum polarization in a system with polarization cross-talk levels of essentially 100\%. In these data the continuum polarization can be recovered to one percent accuracy because of synchronized charge-shuffling model now working with our CCD detector. These techniques can be applied to other spectropolarimeters on other telescopes for both night and day-time applications such as DKIST, TMT and ELT which have folded non-axially symmetric foci.Comment: Accepted to A&

The Origin of Double-Peaked Narrow Lines in Active Galactic Nuclei III: Feedback from Biconical AGN Outflows

We apply an analytic Markov Chain Monte Carlo model to a sample of 18 AGN-driven biconical outflows that we identified from a sample of active galaxies with double-peaked narrow emission lines at z < 0.1 in the Sloan Digital Sky Survey. We find that 8/18 are best described as asymmetric bicones, 8/18 are nested bicones, and 2/18 are symmetric bicones. From the geometry and kinematics of the models, we find that these moderate-luminosity AGN outflows are large and energetic. The biconical outflows axes are randomly oriented with respect to the photometric major axis of the galaxy, implying a randomly oriented and clumpier torus to collimate the outflow, but the torus also allows some radiation to escape equatorially. We find that 16/18 (89%) outflows are energetic enough to drive a two-staged feedback process in their host galaxies. All of these outflows geometrically intersect the photometric major axis of the galaxy, and 23% of outflow host galaxies are significantly redder or have significantly lower specific star formation rates when compared to a matched sample of active galaxies.Comment: 32 pages, 11 figures, accepted for publication in MNRAS; See Figure 7 for a summary of the finding

Observational Constraints on General Relativistic Energy Conditions, Cosmic Matter Density and Dark Energy from X-Ray Clusters of Galaxies and Type-Ia Supernovae

New observational constraints on the cosmic matter density $\Omega_m$ and an effectively redshift-independent equation of state parameter $w_x$ of the dark energy are obtained while simultaneously testing the strong and null energy conditions of general relativity on macroscopic scales. The combination of REFLEX X-ray cluster and type-Ia supernova data shows that for a flat Universe the strong energy condition might presently be violated whereas the null energy condition seems to be fulfilled. This provides another observational argument for the present accelerated cosmic expansion and the absence of exotic physical phenomena related to a broken null energy condition. The marginalization of the likelihood distributions is performed in a manner to include a large fraction of the recently discussed possible systematic errors involved in the application of X-ray clusters as cosmological probes. This yields for a flat Universe, $\Omega_m=0.29^{+0.08}_{-0.12}$ and $w_x=-0.95^{+0.30}_{-0.35}$ ($1\sigma$ errors without cosmic variance). The scatter in the different analyses indicates a quite robust result around $w_x=-1$, leaving little room for the introduction of new energy components described by quintessence-like models or phantom energy. The most natural interpretation of the data is a positive cosmological constant with $w_x=-1 or something like it.Comment: 11 pages, 5 figures, Astron. Astrophys. (in press

The Origin of Double-peaked Narrow Lines in Active Galactic Nuclei. IV. Association with Galaxy Mergers

Double-peaked narrow emission lines in active galactic nucleus (AGN) spectra can be produced by AGN outflows, rotation, or dual AGNs, which are AGN pairs in ongoing galaxy mergers. Consequently, double-peaked narrow AGN emission lines are useful tracers of the coevolution of galaxies and their supermassive black holes, as driven by AGN feedback and AGN fueling. We investigate this concept further with follow-up optical longslit observations of a sample of 95 Sloan Digital Sky Survey (SDSS) galaxies that have double-peaked narrow AGN emission lines. Based on a kinematic analysis of the longslit spectra, we confirm previous work that finds that the majority of double-peaked narrow AGN emission lines are associated with outflows. We also find that eight of the galaxies have companion galaxies with line-of-sight velocity separations < 500 km/s and physical separations <30 kpc. Since we find evidence of AGNs in both galaxies, all eight of these systems are compelling dual AGN candidates. Galaxies with double-peaked narrow AGN emission lines occur in such galaxy mergers at least twice as often as typical active galaxies. Finally, we conclude that at least 3% of SDSS galaxies with double-peaked narrow AGN emission lines are found in galaxy mergers where both galaxies are resolved in SDSS imaging.Comment: 14 pages, 2 figures, ApJ in pres

Efficacy of an 8-Week Concurrent Strength and Endurance Training Programme on Hand Cycling Performance

The aim of the present study was to investigate the effects of an 8-week concurrent strength and endurance training programme in comparison to endurance training only on several key determinants of hand cycling performance. Five H4 and five H3 classified hand cyclists with at least one year’s hand cycling training history consented to participate in the study. Subjects underwent a battery of tests to establish body mass, body composition, VO2peak, maximum aerobic power, gross mechanical efficiency, maximal upper body strength, and 30 km time trial performance. Subjects were matched into pairs based upon 30 km time trial performance and randomly allocated to either a concurrent strength and endurance or endurance training only, intervention group. Following an 8-week training programme based upon a conjugated block periodisation model, subjects completed a second battery of tests. A mixed model, 2-way analysis of variance (ANOVA) revealed no significant changes between groups. However, the calculation of effect sizes (ES) revealed that both groups demonstrated a positive improvement in most physiological and performance measures with subjects in the concurrent group demonstrating a greater magnitude of improvement in body composition (ES -0.80 vs. -0.22) maximal aerobic power (ES 0.97 vs. 0.28), gross mechanical efficiency (ES 0.87 vs. 0.63), bench press 1 repetition maximum (ES 0.53 vs. 0.33), seated row 1 repetition maximum (ES 1.42 vs. 0.43), and 30 km time trial performance (ES -0.66 vs. -0.30). In comparison to endurance training only, an 8-week concurrent training intervention based upon a conjugated block periodisation model appears to be a more effective training regime for improving the performance capabilities of hand cyclists

Stabilization of a-conotoxin AuIB: influences of disulfide connectivity and backbone cyclization

a-Conotoxins are peptides isolated from the venom ducts of cone snails that target nicotinic acetylcholine receptors (nAChRs). They are valuable pharmacological tools and have potential applications for treating a range of conditions in humans, including pain. However, like all peptides, conotoxins are susceptible to degradation, and to enhance their therapeutic potential it is important to elucidate the factors contributing to instability and to develop approaches for improving stability. AuIB is a unique member of the a-conotoxin family because the nonnative &quot;ribbon&quot; disulfide isomer exhibits enhanced activity at the nAChR in rat parasympathetic neurons compared with the native &quot;globular&quot; isomer. Here we show that the ribbon isomer of AuIB is also more resistant to disulfide scrambling, despite having a nonnative connectivity and flexible structure. This resistance to disulfide scrambling does not correlate with overall stability in serum because the ribbon isomer is degraded in human serum more rapidly than the globular isomer. Cyclization via the joining of the N- and C-termini with peptide linkers of four to seven amino acids prevented degradation of the ribbon isomer in serum and stabilized the globular isomers to disulfide scrambling. The linker length used for cyclization strongly affected the relative proportions of the disulfide isomers produced by oxidative folding. Overall, the results of this study provide important insights into factors influencing the stability and oxidative folding of a-conotoxin AuIB and might be valuable in the design of more stable antagonists of nAChRs

Polymerase manager protein UmuD directly regulates Escherichia coli DNA polymerase III α binding to ssDNA

Replication by Escherichia coli DNA polymerase III is disrupted on encountering DNA damage. Consequently, specialized Y-family DNA polymerases are used to bypass DNA damage. The protein UmuD is extensively involved in modulating cellular responses to DNA damage and may play a role in DNA polymerase exchange for damage tolerance. In the absence of DNA, UmuD interacts with the α subunit of DNA polymerase III at two distinct binding sites, one of which is adjacent to the single-stranded DNA-binding site of α. Here, we use single molecule DNA stretching experiments to demonstrate that UmuD specifically inhibits binding of α to ssDNA. We predict using molecular modeling that UmuD residues D91 and G92 are involved in this interaction and demonstrate that mutation of these residues disrupts the interaction. Our results suggest that competition between UmuD and ssDNA for α binding is a new mechanism for polymerase exchange