1,082 research outputs found

    "Fiscal Equalization in Japan: Assessment and Recommendations"

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    Intergovernmental fiscal relations in Japan have been strained in recent years. This paper seeks to assess the Japanese equalization transfer in the light of the theory of fiscal federalism. This paper argues that the case for equalization lies in offsetting net fiscal benefit (NFB) differentials across jurisdictions. It has been shown that the case for equalization and its design depend on the type of public good being provided as well as the mode of finance. Moreover, where equalization is called for, its form and level can be very different depending on whether the relevant policy goal is that of fiscal equity or fiscal efficiency. Studying the institutional context, we arrive at the conclusion that the system of equalization transfers in Japan is consistent with the application of those principles.

    Experimental characterization of the fracture properties of pultruded GFRP structural elements

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    Pultruded Fiber Reinforced Polymers (FRPs) are a class of novel composite materials with remarkable strength (comparable or even greater to that of steel) and resistance to environmental effects. However, the strongly orthotropic behavior of these materials and the relatively high deformability and spatial variability in mechanical properties bring challenges to the widespread adoption of these elements in structural applications. To this end, the orientation and distribution of the fibers are the most influential parameters that affect both the ultimate strength and stiffness of the specimens. This work presents an experimental campaign conducted on GFRP specimens in uniaxial tension and 3-point bending; coupon specimens with three different fibers orientations (namely 0, 45, and 90 degrees) were tested to characterize the ultimate strength and failure modes Results of such experimental campaign are first presented, and detailed statistical measures of the so-obtained strength values are presented with the ultimate goal of characterizing the variability in mechanical properties in commercially available profiles

    Comprehensive Functional Analyses of Expressed Sequence Tags in Common Wheat (Triticum aestivum)

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    About 1 million expressed sequence tag (EST) sequences comprising 125.3 Mb nucleotides were accreted from 51 cDNA libraries constructed from a variety of tissues and organs under a range of conditions, including abiotic stresses and pathogen challenges in common wheat (Triticum aestivum). Expressed sequence tags were assembled with stringent parameters after processing with inbuild scripts, resulting in 37 138 contigs and 215 199 singlets. In the assembled sequences, 10.6% presented no matches with existing sequences in public databases. Functional characterization of wheat unigenes by gene ontology annotation, mining transcription factors, full-length cDNA, and miRNA targeting sites were carried out. A bioinformatics strategy was developed to discover single-nucleotide polymorphisms (SNPs) within our large EST resource and reported the SNPs between and within (homoeologous) cultivars. Digital gene expression was performed to find the tissue-specific gene expression, and correspondence analysis was executed to identify common and specific gene expression by selecting four biotic stress-related libraries. The assembly and associated information cater a framework for future investigation in functional genomics

    Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II

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    In mitosis, the Greatwall kinase (called microtubule-associated serine/threonine kinase like [Mastl] in mammals) is essential for prometaphase entry or progression by suppressing protein phosphatase 2A (PP2A) activity. PP2A suppression in turn leads to high levels of Cdk1 substrate phosphorylation. We have used a mouse model with an oocyte-specific deletion of Mastl to show that Mastl-null oocytes resume meiosis I and reach metaphase I normally but that the onset and completion of anaphase I are delayed. Moreover, after the completion of meiosis I, Mastl-null oocytes failed to enter meiosis II (MII) because they reassembled a nuclear structure containing decondensed chromatin. Our results show that Mastl is required for the timely activation of anaphase-promoting complex/cyclosome to allow meiosis I exit and for the rapid rise of Cdk1 activity that is needed for the entry into MII in mouse oocytes
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