The complete mitogenome of Leptestheria brevirostris Barnard, 1924, a rock pool clam shrimp (Branchiopoda: Spinicaudata) from Central District, Botswana

Spinicaudatan clam shrimp are a widespread and diverse group of branchiopod crustaceans, yet few mitochondrial genomes have been published for this taxonomic group. Here, we present the mitogenome of Leptestheria brevirostris from a rock pool ecosystem in Botswana. Massively parallel sequencing of a single specimen facilitated the reconstruction of the species’ 15,579 bp circularized mitogenome. The reconstructed phylogenetic tree confirms that L. brevirostris forms a monophyletic group with other diplostracan branchiopods, and that these are the sister taxon to Notostraca. The mitogenome reconstructed here is the first to be reported from a leptestherid clam shrimp

Characterization of 14 polymorphic microsatellite loci developed for an Afrotherian species endemic to southern Africa, Elephantulus myurus (Macroscelidea : Macroscelididae)

Fourteen microsatellite loci were developed for the eastern rock sengi, Elephantulus myurus Thomas & Schwann, 1906 by incorporating genetic diversity from across its range in South Africa. Sengis are small mammals belonging to the order Macroscelidea, which comprises 19 species, all of which are endemic to Africa. The loci were amplified in 66 individuals from six localities. An average of 10.5 alleles per locus were identified, with observed and expected heterozygosity values ranging from 0.081 – 0.909 and 0.484 – 0.885, respectively. We also investigated cross-species amplification within the family and found variation in amplification success for five different species. The preliminary results from these amplification efforts could aid further studies into aspects of species diversity and biology. The markers described here represent the first set of variable nuclear markers for the genus Elephantulus, and, together with a set of 8 recently developed markers for Rhynchocyon petersi, Bocage 1880, the first markers for the Order Macroscelidea.National Research Foundation of South Africa (BvV), the University of Johannesburg and the DST-NRF SARChi Chair of Behavioural Ecology and Physiology (NCB). Electrophoresis of microsatellite markers was done at the Analytical Facility based at Stellenbosch University.http://link.springer.com/journal/133552018-02-27hb2016Zoology and Entomolog

Missense Pathogenic variants in KIF4A Affect Dental Morphogenesis Resulting in X-linked Taurodontism, Microdontia and Dens-Invaginatus

The etiology of dental anomalies is multifactorial; and genetic and environmental factors that affect the dental lamina have been implicated. We investigated two families of European ancestry in which males were affected by taurodontism, microdontia and dens invaginatus. In both families, males were related to each other via unaffected females. A linkage analysis was conducted in a New Zealand family, followed by exome sequencing and focused analysis of the X-chromosome. In a US family, exome sequencing of the X-chromosome was followed by Sanger sequencing to conduct segregation analyses. We identified two independent missense variants in KIF4A that segregate in affected males and female carriers. The variant in a New Zealand family (p.Asp371His) predicts the substitution of a residue in the motor domain of the protein while the one in a US family (p.Arg771Lys) predicts the substitution of a residue in the domain that interacts with Protein Regulator of Cytokinesis 1 (PRC1). We demonstrated that the gene is expressed in the developing tooth bud during development, and that the p.Arg771Lys variant influences cell migration in an in vitro assay. These data implicate missense variations in KIF4A in a pathogenic mechanism that causes taurodontism, microdontia and dens invaginatus phenotypes

Preparation, analysis, and application of coated glass targets for the Wendelstein 7-X laser blow-off system

Coated glass targets are a key component of the Wendelstein 7-X laser blow-off system that is used for impurity transport studies. The preparation and analysis of these glass targets as well as their performance is examined in this paper. The glass targets have a high laser damage threshold and are coated via physical vapor deposition with μm thick films. In addition, nm-thin layers of Ti are used as an interface layer for improved ablation efficiency and reduced coating stress. Hence, the metallic or ceramic coating has a lateral homogeneity within 2% and contaminants less than 5%, being optimal for laser ablation processing. With this method, a short (few ms) and well defined pulse of impurities with about 1017 particles can be injected close to the last closed flux surface of Wendelstein 7-X. In particular, a significant amount of atoms with a velocity of about 1 km/s enters the plasma within 1 ms. The atoms are followed by a negligible concentration of slower clusters and macro-particles. This qualifies the use of the targets and applied laser settings for impurity transport studies with the laser blow-off system in Wendelstein 7-X. © 2020 Author(s)

Repeated-root cyclic and negacyclic codes over a finite chain ring

AbstractWe show that repeated-root cyclic codes over a finite chain ring are in general not principally generated. Repeated-root negacyclic codes are principally generated if the ring is a Galois ring with characteristic a power of 2. For any other finite chain ring they are in general not principally generated. We also prove results on the structure, cardinality and Hamming distance of repeated-root cyclic and negacyclic codes over a finite chain ring

In-out charge exchange measurements and 3D modelling of diagnostic thermal neutrals to study edge poloidal impurity asymmetries

A new method was developed to model the neutral population produced by the gas puff based charge exchange recombination spectroscopy systems at ASDEX Upgrade (AUG). With this method, the edge impurity density on the high field side (HFS) and low field side (LFS) can be obtained without the need to apply a neutral beam injection system. The neutral penetration needed for the calculation of the impurity density is obtained with a new gas puff module implemented in the FIDASIM code. The LFS impurity density profile evaluated with the new gas puff module matches the impurity density calculated with standard beam-based charge exchange diagnostics. Impurity temperature, rotation and density profiles at the HFS and LFS of an AUG H-mode discharge are presented. Edge impurity toroidal and poloidal flows show asymmetric structures. The impurity density asymmetries obtained with the new gas puff module are consistent with the observed flow structure