167 research outputs found
Photoionization Broadening of the 1S-2S Transition in a Beam of Atomic Hydrogen
We consider the excitation dynamics of the two-photon \sts transition in a
beam of atomic hydrogen by 243 nm laser radiation. Specifically, we study the
impact of ionization damping on the transition line shape, caused by the
possibility of ionization of the 2S level by the same laser field. Using a
Monte-Carlo simulation, we calculate the line shape of the \sts transition for
the experimental geometry used in the two latest absolute frequency
measurements (M. Niering {\it et al.}, PRL 84, 5496 (2000) and M. Fischer {\it
et al.}, PRL 92, 230802 (2004)). The calculated line shift and line width are
in excellent agreement with the experimentally observed values. From this
comparison we can verify the values of the dynamic Stark shift coefficient for
the \sts transition for the first time on a level of 15%. We show that the
ionization modifies the velocity distribution of the metastable atoms, the line
shape of the \sts transition, and has an influence on the derivation of its
absolute frequency.Comment: 10 pages, 5 figure
Prospects for precision measurements of atomic helium using direct frequency comb spectroscopy
We analyze several possibilities for precisely measuring electronic
transitions in atomic helium by the direct use of phase-stabilized femtosecond
frequency combs. Because the comb is self-calibrating and can be shifted into
the ultraviolet spectral region via harmonic generation, it offers the prospect
of greatly improved accuracy for UV and far-UV transitions. To take advantage
of this accuracy an ultracold helium sample is needed. For measurements of the
triplet spectrum a magneto-optical trap (MOT) can be used to cool and trap
metastable 2^3S state atoms. We analyze schemes for measuring the two-photon
interval, and for resonant two-photon excitation to high
Rydberg states, . We also analyze experiments on the
singlet-state spectrum. To accomplish this we propose schemes for producing and
trapping ultracold helium in the 1^1S or 2^1S state via intercombination
transitions. A particularly intriguing scenario is the possibility of measuring
the transition with extremely high accuracy by use of
two-photon excitation in a magic wavelength trap that operates identically for
both states. We predict a ``triple magic wavelength'' at 412 nm that could
facilitate numerous experiments on trapped helium atoms, because here the
polarizabilities of the 1^1S, 2^1S and 2^3S states are all similar, small, and
positive.Comment: Shortened slightly and reformatted for Eur. Phys. J.
Hemolysis Is Associated with Low Reticulocyte Production Index and Predicts Blood Transfusion in Severe Malarial Anemia
Background: Falciparum Malaria, an infectious disease caused by the apicomplexan parasite Plasmodium falciparum, is among the leading causes of death and morbidity attributable to infectious diseases worldwide. In Gabon, Central Africa, one out of four inpatients have severe malarial anemia (SMA), a life-threatening complication if left untreated. Emerging drug resistant parasites might aggravate the situation. This case control study investigates biomarkers of enhanced hemolysis in hospitalized children with either SMA or mild malaria (MM). Methods and Findings: Ninety-one children were included, thereof 39 SMA patients. Strict inclusion criteria were chosen to exclude other causes of anemia. At diagnosis, erythrophagocytosis (a direct marker for extravascular hemolysis, EVH) was enhanced in SMA compared to MM patients (5.0 arbitrary units (AU) (interquartile range (IR): 2.2–9.6) vs. 2.1 AU (IR: 1.3–3.9), p<0.01). Furthermore, indirect markers for EVH, (i.e. serum neopterin levels, spleen size enlargement and monocyte pigment) were significantly increased in SMA patients. Markers for erythrocyte ageing, such as CD35 (complement receptor 1), CD55 (decay acceleration factor) and phosphatidylserine exposure (annexin-V-binding) were investigated by flow cytometry. In SMA patients, levels of CD35 and CD55 on the red blood cell surface were decreased and erythrocyte removal markers were increased when compared to MM or reconvalescent patients. Additionally, intravascular hemolysis (IVH) was quantified using several indirect markers (LDH, alpha-HBDH, haptoglobin and hemopexin), which all showed elevated IVH in SMA. The presence of both IVH and EVH predicted the need for blood transfusion during antimalarial treatment (odds ratio 61.5, 95% confidence interval (CI): 8.9–427). Interestingly, this subpopulation is characterized by a significantly lowered reticulocyte production index (RPI, p<0.05). Conclusions: Our results show the multifactorial pathophysiology of SMA, whereby EVH and IVH play a particularly important role. We propose a model where removal of infected and non-infected erythrocytes of all ages (including reticulocytes) by EVH and IVH is a main mechanism of SMA. Further studies are underway to investigate the mechanism and extent of reticulocyte removal to identify possible interventions to reduce the risk of SMA development
Two-photon excitation dynamics in bound two-body Coulomb systems including ac Stark shift and ionization
One of the dominant systematic effects that shift resonance lines in high-precision measurements of twophoton transitions is the dynamic (ac) Stark shift. For suitable laser frequencies, the ac Stark shift acquires an imaginary part which corresponds to the rate of resonant one-photon ionization of electrons into a continuum state. At the current level of spectroscopic accuracy, the underlying time-dependent quantum dynamics governing the atomic two-photon excitation process must be well understood, and related considerations are the subject of the present paper. In order to illustrate the basic mechanisms in the transient regime, we investigate an analytically solvable model scenario for the population dynamics in the density matrix formalism and describe in detail how to generalize the corresponding equations of motion for individual experimental use. We also calculate the dynamic Stark shift for two-photon S-S and S-D transitions in bound two-body Coulomb systems and the corresponding two-photon transition matrix elements. In particular, we investigate transitions for which the 1S ground state or alternatively the metastable 2S state acts as the lower-energy state, and for which states with n </= 20 represent the upper states. Relativistic and radiative corrections to the excitation dynamics, and the corresponding limitations to the accuracy of the measurements, are briefly discussed. Our considerations suggest the general feasibility of a detection mechanism, offering high quantum efficiency, based on two-step three-photon resonant ionization spectroscopy, for large classes of experimentally relevant two-photon transitions in two-body Coulomb systems.Peer reviewedPhysic
Systems analysis and controlled malaria infection in Europeans and Africans elucidate naturally acquired immunity
Controlled human infections provide opportunities to study the interaction between the immune system and malaria parasites, which is essential for vaccine development. Here, we compared immune signatures of malaria-naive Europeans and of Africans with lifelong malaria exposure using mass cytometry, RNA sequencing and data integration, before and 5 and 11 days after venous inoculation with Plasmodium falciparum sporozoites. We observed differences in immune cell populations, antigen-specific responses and gene expression profiles between Europeans and Africans and among Africans with differing degrees of immunity. Before inoculation, an activated/differentiated state of both innate and adaptive cells, including elevated CD161(+)CD4(+) T cells and interferon-gamma production, predicted Africans capable of controlling parasitemia. After inoculation, the rapidity of the transcriptional response and clusters of CD4(+) T cells, plasmacytoid dendritic cells and innate T cells were among the features distinguishing Africans capable of controlling parasitemia from susceptible individuals. These findings can guide the development of a vaccine effective in malaria-endemic regions.Malaria immunity can be acquired through natural infection, but the correlates of protection are still being determined. Yazdanbakhsh and colleagues combine experimental infection of volunteers with Plasmodium falciparum with systems analysis to throw light on the nature of protective immune responses.Radiolog
Are Consumer Expectations Theory-Consistent? The Role of Macroeconomic Determinants and Central Bank Communication
Savings and Investment Correlations in Response to Monetary Policy Shocks: New Insights into the Feldstein-Horioka Puzzle?
Isolation, production and characterization of fully human monoclonal antibodies directed to Plasmodium falciparum MSP10
The reaction of NADPH with bovine mitochondrial NADH:ubiquinone oxidoreductase revisited
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