68 research outputs found
Determination Of Flavanones In Orange Juices Obtained From Different Sources By Hplc/dad.
Flavanones (hesperidin, naringenin, naringin, and poncirin) in industrial, hand-squeezed orange juices and from fresh-in-squeeze machines orange juices were determined by HPLC/DAD analysis using a previously described liquid-liquid extraction method. Method validation including the accuracy was performed by using recovery tests. Samples (36) collected from different Brazilian locations and brands were analyzed. Concentrations were determined using an external standard curve. The limits of detection (LOD) and the limits of quantification (LOQ) calculated were 0.0037, 1.87, 0.0147, and 0.0066 mg 100 g(-1) and 0.0089, 7.84, 0.0302, and 0.0200 mg 100 g(-1) for naringin, hesperidin, poncirin, and naringenin, respectively. The results demonstrated that hesperidin was present at the highest concentration levels, especially in the industrial orange juices. Its average content and concentration range were 69.85 and 18.80-139.00 mg 100 g(-1). The other flavanones showed the lowest concentration levels. The average contents and concentration ranges found were 0.019, 0.01-0.30, and 0.12 and 0.1-0.17, 0.13, and 0.01-0.36 mg 100 g(-1), respectively. The results were also evaluated using the principal component analysis (PCA) multivariate analysis technique which showed that poncirin, naringenin, and naringin were the principal elements that contributed to the variability in the sample concentrations.201429683
Physical characterisation of an alginate/lysozyme nano-laminate coating and its evaluation on ‘coalho’ cheese shelf life
This work aimed at the characterisation of a nanolaminate
coating produced by the layer-by-layer methodology
and its evaluation on the preservation of ‘Coalho’ cheese.
Initially, five alternate layers of alginate and lysozyme were
assembled in an aminolysed/charged polyethylene terephthalate
(A/C PET) and physically characterised by UV/VIS
spectroscopy, contact angle, water vapour (WVTR) and oxygen
(OTR) transmission rates and scanning electron microscopy.
Afterwards, the same methodology was used to
apply the nano-laminate coating in ‘Coalho’ cheese and its
shelf life was evaluated during 20 days in terms of mass
loss, pH, lipid peroxidation, titratable acidity and microbial
count. UV/VIS spectroscopy and contact angle analyses
confirmed the layers’ deposition and the successful assembly
of nano-laminate coating on A/C PET surface. The coating
presented WVTR and OTR values of 1.03×10−3 and 1.28×
10−4 g m−2 s−1, respectively. After 20 days, coated cheese
showed lower values of mass loss, pH, lipidic peroxidation,
microorganisms’ proliferation and higher titratable acidity in
comparison with uncoated cheese. These results suggest that
gas barrier and antibacterial properties of alginate/lysozyme
nanocoating can be used to extend the shelf life of ‘Coalho’
cheese.The author Bartolomeu G. de S. Medeiros is recipient of a scholarship from Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES-Brazil). The author Marthyna P. Souza is recipient of a scholarship from Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE, Brazil) and was recipient of a scholarship from Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES/PDEE-Brazil). The authors Ana C. Pinheiro, Ana I. Bourbon and Miguel A. Cerqueira are recipients of a fellowship (SFRH/BD/48120/2008, SFRH/BD/73178/2010 and SFRH/BPD/72753/2010, respectively), supported by Fundacao para a Ciencia e Tecnologia, POPH-QREN and FSE (FCT, Portugal). Maria G. Carneiro-da-Cunha express is gratitude to the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) for research grant. The present work was supported by CAPES/PROCAD/NF/1415/2007. The support of EU Cost Action FA0904 is gratefully acknowledged
Seasonality of reproduction of epiphytic bryophytes in flooded forests from the Caxiuanã National Forest, Eastern Amazon
Unraveling the myriad contributors to persistent diminished exercise capacity after critical illness
Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in rats
Pawedness Trait Test (PaTRaT)-A New Paradigm to Evaluate Paw Preference and Dexterity in RatsIn rodents, dexterity is commonly analyzed in preference paradigms in which animals are given the chance to use either the left or the right front paws to manipulate food. However, paw preference and dexterity at population and individual levels are controversial as results are incongruent across paradigms. We have therefore developed a semi-quantitative method-the pawdeness trait test (PaTRaT)-to evaluate paw preference degree in rats. The PaTRaT consists in a classification system, ranging from +4 to 4 where increasingly positive and negative values reflect the bias for left or right paw use, respectively. Sprague-Dawley male rats were confined into a metal rectangular mesh cylinder, from which they can see, smell and reach sugared rewards with their paws. Due to its size, the reward could only cross the mesh if aligned with its diagonal, imposing additional coordination. Animals were allowed to retrieve 10 rewards per session in a total of four sessions while their behavior was recorded. PaTRaT was repeated 4 and 8 weeks after the first evaluation. To exclude potential bias, rats were also tested for paw fine movement and general locomotion in other behavioral paradigms as well as impulsivity (variable delay-to-signal, VDS), memory and cognitive flexibility (water maze). At the population level 54% of the animals presented a rightward bias. Individually, all animals presented marked side-preferences, >2 and <-2 for left-and right-sided bias, respectively, and this preference was stable across the three evaluations. Inter-rater consistency was very high between two experienced raters and substantial when two additional inexperienced raters were included. Left-and right-biased animals presented no differences in the ability to perform fine movements with any of the forelimbs (staircase) and general locomotor performance. Additionally, these groups performed similarly in executive function and memory tasks. In conclusion, PaTRaT is able to reliably classify rats' pawedness direction and degree.This work has been funded by the European Regional Development Fund (FEDER), through the Competitiveness Factors Operational Programme (COMPETE) and the Northern Portugal Regional Operational Programme (NORTE 2020) under the Portugal 2020 Partnership Agreement (project NORTE-01-0145-FEDER-000023). It was also funded by National funds, through the Foundation for Science and Technology (Fundacao para a Ciencia e a Tecnologia, FCT), under the scope of the projects POCI-01-0145-FEDER-007038 and PTDC/NEU-SCC/5301/2014. Researchers were supported by FCT grant numbers SFRH/BD/109111/2015 (AMC), SFRH/BD/52291/2013 (ME via Inter-University Doctoral Programme in Ageing and Chronic Disease, PhDOC), PD/BD/114120/2015 (SPN via PhDOC), SFRH/BD/89936/2012 (SB), PD/BD/114117/2015 (MRG via PhDOC) and SFRH/BPD/80118/2011 (HL-A).info:eu-repo/semantics/publishedVersio
EM TEMPOS GLOBAIS, UM “NOVO” LOCAL: a Ford na Bahia
O artigo analisa a dinâmica da Região Metropolitana de Salvador (RMS) a partir da implantação da Ford, discutindo a perspectiva do ‘lugar’ (a periferia metropolitana), dentro de uma relação assimétrica com os negócios globais na era da flexibilidade. O texto caracteriza o complexo Ford de Camaçari a partir da reestruturação produtiva e das mudanças na organização e funcionamento dos territórios e, na segunda parte, seus impactos sobre a periferia metropolitana de Salvador. Na conclusão demonstra que as mesmas circunstâncias que permitiram a vinda da montadora para Camaçari constrangem as ambições originais de melhor equacionamento entre crescimento econômico e progresso social: a flexibilidade dos novos arranjos, que tornam os espaços periféricos estratégicos, compromete o “enraizamento” do investimento; a “produção enxuta”, exígua de emprego e diligente na sua precarização, inibe os benefícios sociais. PALAVRAS CHAVE: reestruturação produtiva, mercado de trabalho, indústria automobilística, periferia metropolitana, segregação socioespacial. IN GLOBAL TIMES, A “NEW” PLACE: Ford in Bahia Ângela Franco This paper makes an analysis of the dynamics of the Metropolitan Area of Salvador (in Portuguese, RMS) starting from the implantation of Ford, discussing the perspective of the ‘local’ (the metropolitan periphery), inside of an asymmetrical relationship with global businesses in the age of flexibility. The Ford Automotive Compound is caracterized in the first part of the paper from its productive reestructuring and changes in the organization and work of territories, and, in the second part, from its impact on the the metropolitan periphery from Salvador. In its conclusion it demonstrates that the same circumstances that allowed the arrival of the automotive maker in Camaçari constrain the original ambitions of better ratio between economical growth and social progress: the flexibility of the new automotive production methods, making peripheric spaces strategic, compromises on the permanence of the investments; and the “streamlined production”, easy on job production and hard on job flexibilization inhibit social benefits. KEYWORDS: productive restructuring, job market, automobile industry, metropolitan periphery, socioespatial segregation. EN PERIODE DE MONDIALISATION, UN “NOUVEAU” LOCAL: Ford à Bahia Ângela Franco Cet article traite de l’analyse de la dynamique de la Région Métropolitaine de Salvador (RMS), à partir de l’implantation de l’usine Ford. On y discute de la perspective du “lieu” (la périphérie métropolitaine), dans une relation asymétrique avec les affaires globales à une époque de flexibilité. On y caractérise le complexe Ford de Camaçari à partir de la restructuration productive et des changements dans l’organisation et le fonctionnement des territoires. Ses impacts sur la périphérie métropolitaine de Salvador sont présentés dans la deuxième partie. En conclusion, on y démontre que ce sont les mêmes circonstances qui ont permis l’arrivée de l’usine de montage à Camaçari qui représentent une contrainte pour les ambitions qui, à l’origine, voulaient atteindre une meilleure équation entre la croissance économique et le progrès social. La flexibilité de ces nouveaux arrangements, qui rendent les espaces périphériques stratégiques, compromet “l’enracinement” des investissements, la “production exiguë”, l’exiguïté des emplois et la diligence dans leur précarisation, elle inhibe les avantages sociaux. MOTS-CLÉS: restructuration productive, marché du travail, industrie automobile, périphérie métropolitaine, ségrégation sociale et spatiale. Publicação Online do Caderno CRH: http://www.cadernocrh.ufba.b
Tau-dependent suppression of adult neurogenesis in the stressed hippocampus
uncorrected proofStress, a well-known sculptor of brain plasticity, is shown to suppress hippocampal neurogenesis in the adult brain; yet, the underlying cellular mechanisms are poorly investigated. Previous studies have shown that chronic stress triggers hyperphosphorylation and accumulation of the cytoskeletal protein Tau, a process that may impair the cytoskeleton-regulating role (s) of this protein with impact on neuronal function. Here, we analyzed the role of Tau on stress-driven suppression of neurogenesis in the adult dentate gyrus (DG) using animals lacking Tau (Tau-knockout; Tau-KO) and wild-type (WT) littermates. Unlike WTs, Tau-KO animals exposed to chronic stress did not exhibit reduction in DG proliferating cells, neuroblasts and newborn neurons; however, newborn astrocytes were similarly decreased in both Tau-KO and WT mice. In addition, chronic stress reduced phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR)/glycogen synthase kinase-3 beta (GSK3 beta)/beta-catenin signaling, known to regulate cell survival and proliferation, in the DG of WT, but not Tau-KO, animals. These data establish Tau as a critical regulator of the cellular cascades underlying stress deficits on hippocampal neurogenesis in the adult brain.Portuguese Foundation for Science and Technology (FCT) Investigator grants (IF/01799/2013, IF/00883/2013, IF/01079/2014, respectively). This work was funded by FCT research grants 'PTDC/SAU-NMC/113934/2009' (IS), the Portuguese North Regional Operational Program (ON.2) under the National Strategic Reference Framework (QREN), through the European Regional Development Fund (FEDER), the Project Estratégico co-funded by FCT (PEst-C/SAU/LA0026/2013) and the European Regional Development Fund COMPETE (FCOMP-01-0124-FEDER-037298) as well as the project NORTE-01-0145-FEDER-000013, supported by the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER)info:eu-repo/semantics/publishedVersio
Uncharted waters: rare and unclassified cardiomyopathies characterized on cardiac magnetic resonance imaging
Cardiac magnetic resonance imaging (CMR) has undergone considerable technology advances in recent years, so that it is now entering into mainstream cardiac imaging practice. In particular, CMR is proving to be a valuable imaging tool in the detection, morphological assessment and functional assessment of cardiomyopathies. Although our understanding of this broad group of heart disorders continues to expand, it is an evolving group of entities, with the rarer cardiomyopathies remaining poorly understood or even unclassified. In this review, we describe the clinical and pathophysiological aspects of several of the rare/unclassified cardiomyopathies and their appearance on CMR
Optically controlled reconfigurable antenna for 5G future broadband cellular communication networks
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