1,209 research outputs found
Jet engine powers large, high-temperature wind tunnel
Wind tunnel for large component testing uses a jet engine with afterburner to provide high temperatures /1200 degrees to 2000 degrees F/ and controlled high velocity gas. This economical wind tunnel can accommodate parts ten feet by ten feet or larger, and is a useful technique for qualitative information
Pulsation period variations in the RRc Lyrae star KIC 5520878
Learned et. al. proposed that a sufficiently advanced extra-terrestrial
civilization may tickle Cepheid and RR Lyrae variable stars with a neutrino
beam at the right time, thus causing them to trigger early and jogging the
otherwise very regular phase of their expansion and contraction. This would
turn these stars into beacons to transmit information throughout the galaxy and
beyond. The idea is to search for signs of phase modulation (in the regime of
short pulse duration) and patterns, which could be indicative of intentional,
omnidirectional signaling.
We have performed such a search among variable stars using photometric data
from the Kepler space telescope. In the RRc Lyrae star KIC 5520878, we have
found two such regimes of long and short pulse durations. The sequence of
period lengths, expressed as time series data, is strongly auto correlated,
with correlation coefficients of prime numbers being significantly higher
(\%). Our analysis of this candidate star shows that the prime number
oddity originates from two simultaneous pulsation periods and is likely of
natural origin.
Simple physical models elucidate the frequency content and asymmetries of the
KIC 5520878 light curve.
Despite this SETI null result, we encourage testing other archival and future
time-series photometry for signs of modulated stars. This can be done as a
by-product to the standard analysis, and even partly automated.Comment: Accepted for publication in ApJ. 49 pages, 16 figure
Nonlinear Mechanical Response of DNA due to Anisotropic Bending Elasticity
The response of a short DNA segment to bending is studied, taking into
account the anisotropy in the bending rigidities caused by the double-helical
structure. It is shown that the anisotropy introduces an effective nonlinear
twist-bend coupling that can lead to the formation of kinks and modulations in
the curvature and/or in the twist, depending on the values of the elastic
constants and the imposed deflection angle. The typical wavelength for the
modulations, or the distance between the neighboring kinks is found to be set
by half of the DNA pitch.Comment: 4 pages, 3 encapsulated EPS figure
A stitch in time: Efficient computation of genomic DNA melting bubbles
Background: It is of biological interest to make genome-wide predictions of
the locations of DNA melting bubbles using statistical mechanics models.
Computationally, this poses the challenge that a generic search through all
combinations of bubble starts and ends is quadratic.
Results: An efficient algorithm is described, which shows that the time
complexity of the task is O(NlogN) rather than quadratic. The algorithm
exploits that bubble lengths may be limited, but without a prior assumption of
a maximal bubble length. No approximations, such as windowing, have been
introduced to reduce the time complexity. More than just finding the bubbles,
the algorithm produces a stitch profile, which is a probabilistic graphical
model of bubbles and helical regions. The algorithm applies a probability peak
finding method based on a hierarchical analysis of the energy barriers in the
Poland-Scheraga model.
Conclusions: Exact and fast computation of genomic stitch profiles is thus
feasible. Sequences of several megabases have been computed, only limited by
computer memory. Possible applications are the genome-wide comparisons of
bubbles with promotors, TSS, viral integration sites, and other melting-related
regions.Comment: 16 pages, 10 figure
Improving Behaviour in Schools: Evidence Review
This is the final version. Available from the Education Endowment Foundation via the link in this record"Improving Behaviour in Schools: Guidance Report" is available in ORE at http://hdl.handle.net/10871/37789Education Endowment Foundatio
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Interplay between DNA sequence and negative superhelicity drives R-loop structures.
R-loops are abundant three-stranded nucleic-acid structures that form in cis during transcription. Experimental evidence suggests that R-loop formation is affected by DNA sequence and topology. However, the exact manner by which these factors interact to determine R-loop susceptibility is unclear. To investigate this, we developed a statistical mechanical equilibrium model of R-loop formation in superhelical DNA. In this model, the energy involved in forming an R-loop includes four terms-junctional and base-pairing energies and energies associated with superhelicity and with the torsional winding of the displaced DNA single strand around the RNA:DNA hybrid. This model shows that the significant energy barrier imposed by the formation of junctions can be overcome in two ways. First, base-pairing energy can favor RNA:DNA over DNA:DNA duplexes in favorable sequences. Second, R-loops, by absorbing negative superhelicity, partially or fully relax the rest of the DNA domain, thereby returning it to a lower energy state. In vitro transcription assays confirmed that R-loops cause plasmid relaxation and that negative superhelicity is required for R-loops to form, even in a favorable region. Single-molecule R-loop footprinting following in vitro transcription showed a strong agreement between theoretical predictions and experimental mapping of stable R-loop positions and further revealed the impact of DNA topology on the R-loop distribution landscape. Our results clarify the interplay between base sequence and DNA superhelicity in controlling R-loop stability. They also reveal R-loops as powerful and reversible topology sinks that cells may use to nonenzymatically relieve superhelical stress during transcription
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Scott Polar Research Institute Arctic Airborne Radio-Echo Sounding Datasets: Canadian Arctic 2000
This data set contains radar sounder profiles from the SPRI 100 MHz ice-penetrating radar instrument over Devon and Ellesmere islands in the Canadian Arctic (CA). Along with the radar profile data, the archive includes derived and ancillary data, plus software code produced for acquisition and working up of data. The data were collected under funding from a UK Natural Environment Research Council (NERC) grant (GR3/12469) to Prof. J. A. Dowdeswell. Please consult the 'MetadataCA2000.pdf' file for a detailed description of the dataset and its contents.Funding: The collection of these data was funded by NERC grant GR3/12469 to J.A. Dowdeswell, as well as by grants from the Meteorological Service of Canada (CRYSYS program) to M.J. Sharp. Further funding (to J.A. Dowdeswell) for development of the dataset was provided by the NERC Centre for Polar Observation and Modelling, UK, and the EU SPICE Project
Conformations of closed DNA
We examine the conformations of a model for a short segment of closed DNA.
The molecule is represented as a cylindrically symmetric elastic rod with a
constraint corresponding to a specification of the linking number. We obtain
analytic expressions leading to the spatial configuration of a family of
solutions representing distortions that interpolate between the circular form
of DNA and a figure-eight form that represents the onset of interwinding. We
are also able to generate knotted loops. We suggest ways to use our approach to
produce other configurations relevant to studies of DNA structure. The
stability of the distorted configurations is assessed, along with the effects
of fluctuations on the free energy of the various configurations.Comment: 39 pages in REVTEX with 14 eps figures. Submitted to Phys. Rev. E.
This manuscript updates, expands and revises, to a considerable extent, a
previously posted manuscript, entitled "Conformations of Circular DNA," which
appeared as cond-mat/970104
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